Click here to switch to the map view.

The map label for this gene is argF

Identifier: 121997523

GI number: 121997523

Start: 811770

End: 812672

Strand: Reverse

Name: argF

Synonym: Hhal_0726

Alternate gene names: 121997523

Gene position: 812672-811770 (Counterclockwise)

Preceding gene: 121997524

Following gene: 121997522

Centisome position: 30.34

GC content: 70.43

Gene sequence:

>903_bases
ATGCGCCACTTCCTCACCCTGCGTGACTGCTCCGTCGACGAGATCCACCAGCTGCTGCGCCGGGCCACGGAGCTGCGCGA
CATGCACCGTGCCGGGCATCTGCACCAGCCCCTGCAGGGCCGGGTGCTGGGGATGGTCTTCGAGAAATCCTCCACCCGCA
CCCGGGTCTCCTTCGAGGCCGGCATGGCGCAGCTCGGGGGCCACGCCCTGTTCCTCTCGCCGCGGGATACCCAGCTTGGG
CGCGGCGAGCCGGTGGAGGACACCGCCCGGGTGGTCTCGCGCATGGTCGATGCGGTGATGATCCGCACCTTCGGCCACGA
TATGCTGGAGCGCTTCGCCGGCCACTCGGCGGCGCCGGTGATCAACGGCCTCTCGGACCTGTGCCACCCGTGCCAGCTGC
TGGCCGATCTGCAGACCTTCACCGAGCACCGCGGGCCGATCACCGACCGGCGCGTGGCGTGGATCGGCGACGGCAACAAC
ATGTGCCATAGCTGGATCGAGGCCGCTGGCCTGCTCGGCTTCGACCTGCACATCGCCGCGCCGCCGGGGTACCGCCCGGA
GGCGGACATCCTGGCGCTGGGCGGTGACCGCGTCCAGGTCTTCGACGACGCCTACGCCGCCGCCGAGGGCGCCGACCTGG
TCACCACCGACGTCTGGGCGAGCATGGGCCAGGAGAGCGAGCAGGCGGCCCGCGAGGAGGCCTTCCGCGGCTTCTGCGTG
ACCGAGTCGCTGATGGAGTGTGCCGGCGAGCAGGCGCTGTTCATGCACTGCCTGCCGGCCCACCGGGGCGAGGAGGTGGA
GGCCGCGGTGCTCGAAGGACCGCAGAGCGTCGTCTGGGACGAGGCGGAGAATCGCCTGCACGCCCAGAAGGCGCTGCTTG
AATTCCTGATGCTGGGATACTGA

Upstream 100 bases:

>100_bases
ACGATGATGAGATCCGCCAGCTCGCCGACGGCGTCGTCGACCTAGTGCGCCGCCACCTGGCCGGCGCCTGACCTGAACCG
AACCAAGGAGGGCGTCCTGG

Downstream 100 bases:

>100_bases
CCCCTGAACCTGAATACCGAGGAGCTCCCCATGTCGGACGTCAAGAAGGTCGTACTCGCCTACTCCGGCGGTCTCGACAC
CTCCGTGATCCTCAAGTGGC

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MRHFLTLRDCSVDEIHQLLRRATELRDMHRAGHLHQPLQGRVLGMVFEKSSTRTRVSFEAGMAQLGGHALFLSPRDTQLG
RGEPVEDTARVVSRMVDAVMIRTFGHDMLERFAGHSAAPVINGLSDLCHPCQLLADLQTFTEHRGPITDRRVAWIGDGNN
MCHSWIEAAGLLGFDLHIAAPPGYRPEADILALGGDRVQVFDDAYAAAEGADLVTTDVWASMGQESEQAAREEAFRGFCV
TESLMECAGEQALFMHCLPAHRGEEVEAAVLEGPQSVVWDEAENRLHAQKALLEFLMLGY

Sequences:

>Translated_300_residues
MRHFLTLRDCSVDEIHQLLRRATELRDMHRAGHLHQPLQGRVLGMVFEKSSTRTRVSFEAGMAQLGGHALFLSPRDTQLG
RGEPVEDTARVVSRMVDAVMIRTFGHDMLERFAGHSAAPVINGLSDLCHPCQLLADLQTFTEHRGPITDRRVAWIGDGNN
MCHSWIEAAGLLGFDLHIAAPPGYRPEADILALGGDRVQVFDDAYAAAEGADLVTTDVWASMGQESEQAAREEAFRGFCV
TESLMECAGEQALFMHCLPAHRGEEVEAAVLEGPQSVVWDEAENRLHAQKALLEFLMLGY
>Mature_300_residues
MRHFLTLRDCSVDEIHQLLRRATELRDMHRAGHLHQPLQGRVLGMVFEKSSTRTRVSFEAGMAQLGGHALFLSPRDTQLG
RGEPVEDTARVVSRMVDAVMIRTFGHDMLERFAGHSAAPVINGLSDLCHPCQLLADLQTFTEHRGPITDRRVAWIGDGNN
MCHSWIEAAGLLGFDLHIAAPPGYRPEADILALGGDRVQVFDDAYAAAEGADLVTTDVWASMGQESEQAAREEAFRGFCV
TESLMECAGEQALFMHCLPAHRGEEVEAAVLEGPQSVVWDEAENRLHAQKALLEFLMLGY

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI38788445, Length=307, Percent_Identity=41.3680781758958, Blast_Score=240, Evalue=1e-63,
Organism=Homo sapiens, GI18105007, Length=305, Percent_Identity=28.1967213114754, Blast_Score=99, Evalue=5e-21,
Organism=Escherichia coli, GI1790703, Length=322, Percent_Identity=36.6459627329193, Blast_Score=207, Evalue=6e-55,
Organism=Escherichia coli, GI1786469, Length=322, Percent_Identity=36.6459627329193, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI2367364, Length=316, Percent_Identity=28.7974683544304, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI48994908, Length=362, Percent_Identity=23.7569060773481, Blast_Score=72, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI193204318, Length=172, Percent_Identity=32.5581395348837, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6322373, Length=319, Percent_Identity=33.2288401253918, Blast_Score=198, Evalue=9e-52,
Organism=Saccharomyces cerevisiae, GI6322331, Length=310, Percent_Identity=27.741935483871, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24642586, Length=271, Percent_Identity=26.9372693726937, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): OTC_HALHL (A1WUZ6)

Other databases:

- EMBL:   CP000544
- RefSeq:   YP_001002310.1
- ProteinModelPortal:   A1WUZ6
- SMR:   A1WUZ6
- STRING:   A1WUZ6
- GeneID:   4711349
- GenomeReviews:   CP000544_GR
- KEGG:   hha:Hhal_0726
- eggNOG:   COG0078
- HOGENOM:   HBG579429
- OMA:   CVVTDTW
- PhylomeDB:   A1WUZ6
- ProtClustDB:   PRK00779
- BioCyc:   HHAL349124:HHAL_0726-MONOMER
- HAMAP:   MF_01109
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292
- PRINTS:   PR00100
- PRINTS:   PR00102
- TIGRFAMs:   TIGR00658

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.3

Molecular weight: Translated: 33169; Mature: 33169

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: BINDING 102-102 BINDING 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHFLTLRDCSVDEIHQLLRRATELRDMHRAGHLHQPLQGRVLGMVFEKSSTRTRVSFEA
CCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCEEHEEEECCCCCEEEEHHH
GMAQLGGHALFLSPRDTQLGRGEPVEDTARVVSRMVDAVMIRTFGHDMLERFAGHSAAPV
HHHHHCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
INGLSDLCHPCQLLADLQTFTEHRGPITDRRVAWIGDGNNMCHSWIEAAGLLGFDLHIAA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCEEEEEEC
PPGYRPEADILALGGDRVQVFDDAYAAAEGADLVTTDVWASMGQESEQAAREEAFRGFCV
CCCCCCCCCEEEECCCEEEEEHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCHHH
TESLMECAGEQALFMHCLPAHRGEEVEAAVLEGPQSVVWDEAENRLHAQKALLEFLMLGY
HHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRHFLTLRDCSVDEIHQLLRRATELRDMHRAGHLHQPLQGRVLGMVFEKSSTRTRVSFEA
CCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCEEHEEEECCCCCEEEEHHH
GMAQLGGHALFLSPRDTQLGRGEPVEDTARVVSRMVDAVMIRTFGHDMLERFAGHSAAPV
HHHHHCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
INGLSDLCHPCQLLADLQTFTEHRGPITDRRVAWIGDGNNMCHSWIEAAGLLGFDLHIAA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCEEEEEEC
PPGYRPEADILALGGDRVQVFDDAYAAAEGADLVTTDVWASMGQESEQAAREEAFRGFCV
CCCCCCCCCEEEECCCEEEEEHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCHHH
TESLMECAGEQALFMHCLPAHRGEEVEAAVLEGPQSVVWDEAENRLHAQKALLEFLMLGY
HHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA