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The map label for this gene is dadA2 [H]

Identifier: 121611644

GI number: 121611644

Start: 5228904

End: 5230130

Strand: Direct

Name: dadA2 [H]

Synonym: Veis_4738

Alternate gene names: 121611644

Gene position: 5228904-5230130 (Clockwise)

Preceding gene: 121611641

Following gene: 121611651

Centisome position: 93.93

GC content: 71.64

Gene sequence:

>1227_bases
ATGCATGTGTGTGTGCTCGGTGCCGGCATCGTGGGCCTGGCCACCGCCTGGCAGCTCGAACGCCGGGGCCACCAGGTCAC
GGTGATCGACCGCGCGGCGCCCGGCGCCGGCGCGAGCGGCGGCAACGGCGCGCAACTGAGCTACTCGTATGTGCAGCCGC
TGGCCGATCCGTCGATCTGGAAGCAGTTGCCCAAGCTGCTGCTCTCGCCCACCTCCCCGCTCAAGCTGCGCCCGCAGCTC
GATCCGCTGCAGTGGCGCTGGGGCATGGCGTTTCTCGCGGCCTGCAATGCGCAGACTTCGCGCGACACCACGGCGAAGCT
GCTGGCGCTGGCCGCGTCGAGCCGCACCGAGTTCGAGGCGATGCAGGCCGACATCGCGCCCGACTGCGACTTCTCGGCCA
CCGGCAAGCTGGTGCTGTACGCGAGCGCGGCTGCGCTCGCGGGCGCGCGTGCGCAGCTCGAACTGCAGCGCACCATGGGC
AGCCAGCAGCGCATCGTCGCGCCCGACGAGTGCATCGCCATCGAACCCGCGCTCGCGAGCTACCGCAGCCAGATGGCCGG
TGCGGTCTACACACCGAGCGAATGCGCGGCCGACTGCCTGAAGGTGTGCGCCGAGCTGATGCGTTCGCTCGAAGCACGCG
GCGTGCGTTTCCTGCCCGGTACCGACGTGCACGGCTTCGCGCGCAATGGCCATCGCATCGCGGCGGTGCACACGAGCCAG
GGCGACATCGAGGCCGATGCCTTCGTGATGGCGCTGGGTTCGGCCTCGCACAAGCTCGGCCGCGCGCTGGGCGCGTATCT
GCCGGTGTACCCGCTCAAGGGCTACAGCATCACGGTGGCGGTCGATCCCTCGCCCGGCGCGGCGCCGACCGTGAACGTGA
CCGACAGCGCCCGCAAGGTGGTGTTCGCGCGCATCGGCTCACGCCTGCGCGTGGCGGGCATGGCCGAACTGGTGGGGCAC
GACGCGGGCATTCCGGCGACACGCATCGCGACGCTGGCAGCCGCCACGCGCGCCCTGTTCCCGCATGCGGGCCGGCTCGA
AGAACTGCACCCGTGGACCGGCATGCGGCCCGCCACGCCCAGGGGCCTGCCGATCATCGGGCGGCTGGCGCGCGCACCGT
CGAACATGCTGTTCAACACCGGCCACGGCGCGCTGGGTTTCACGCTGGCCTTCGGCTCTGCGCTGCGGGTCGCCGAGGCG
CTGGCGTCTTGCGCGCCGACGCCCTGA

Upstream 100 bases:

>100_bases
GGGCGGGGGTTCTTTTGGCATTTTTGGGTGGTGCGGGGCGACACTAGCCTACAGTCGCAGGCAATCCCTTGTTTGTCTCC
ACTCCCGGAAAGTTGCCTTC

Downstream 100 bases:

>100_bases
GCTTGCCGCCCAGGCGCCGCCAGCCGATCACCACGCCGGTCAGGCTCAGCAGCGCGCCGCCGAGACTCAGCGTCACGAGC
AGCATGTCCCACACCGGGCG

Product: D-amino-acid dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MHVCVLGAGIVGLATAWQLERRGHQVTVIDRAAPGAGASGGNGAQLSYSYVQPLADPSIWKQLPKLLLSPTSPLKLRPQL
DPLQWRWGMAFLAACNAQTSRDTTAKLLALAASSRTEFEAMQADIAPDCDFSATGKLVLYASAAALAGARAQLELQRTMG
SQQRIVAPDECIAIEPALASYRSQMAGAVYTPSECAADCLKVCAELMRSLEARGVRFLPGTDVHGFARNGHRIAAVHTSQ
GDIEADAFVMALGSASHKLGRALGAYLPVYPLKGYSITVAVDPSPGAAPTVNVTDSARKVVFARIGSRLRVAGMAELVGH
DAGIPATRIATLAAATRALFPHAGRLEELHPWTGMRPATPRGLPIIGRLARAPSNMLFNTGHGALGFTLAFGSALRVAEA
LASCAPTP

Sequences:

>Translated_408_residues
MHVCVLGAGIVGLATAWQLERRGHQVTVIDRAAPGAGASGGNGAQLSYSYVQPLADPSIWKQLPKLLLSPTSPLKLRPQL
DPLQWRWGMAFLAACNAQTSRDTTAKLLALAASSRTEFEAMQADIAPDCDFSATGKLVLYASAAALAGARAQLELQRTMG
SQQRIVAPDECIAIEPALASYRSQMAGAVYTPSECAADCLKVCAELMRSLEARGVRFLPGTDVHGFARNGHRIAAVHTSQ
GDIEADAFVMALGSASHKLGRALGAYLPVYPLKGYSITVAVDPSPGAAPTVNVTDSARKVVFARIGSRLRVAGMAELVGH
DAGIPATRIATLAAATRALFPHAGRLEELHPWTGMRPATPRGLPIIGRLARAPSNMLFNTGHGALGFTLAFGSALRVAEA
LASCAPTP
>Mature_408_residues
MHVCVLGAGIVGLATAWQLERRGHQVTVIDRAAPGAGASGGNGAQLSYSYVQPLADPSIWKQLPKLLLSPTSPLKLRPQL
DPLQWRWGMAFLAACNAQTSRDTTAKLLALAASSRTEFEAMQADIAPDCDFSATGKLVLYASAAALAGARAQLELQRTMG
SQQRIVAPDECIAIEPALASYRSQMAGAVYTPSECAADCLKVCAELMRSLEARGVRFLPGTDVHGFARNGHRIAAVHTSQ
GDIEADAFVMALGSASHKLGRALGAYLPVYPLKGYSITVAVDPSPGAAPTVNVTDSARKVVFARIGSRLRVAGMAELVGH
DAGIPATRIATLAAATRALFPHAGRLEELHPWTGMRPATPRGLPIIGRLARAPSNMLFNTGHGALGFTLAFGSALRVAEA
LASCAPTP

Specific function: Oxidative deamination of D-amino acids [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DadA oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787438, Length=408, Percent_Identity=33.578431372549, Blast_Score=230, Evalue=1e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023080
- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.4.99.1 [H]

Molecular weight: Translated: 42758; Mature: 42758

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVCVLGAGIVGLATAWQLERRGHQVTVIDRAAPGAGASGGNGAQLSYSYVQPLADPSIW
CEEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCEEEHHHHCCCCCHHHH
KQLPKLLLSPTSPLKLRPQLDPLQWRWGMAFLAACNAQTSRDTTAKLLALAASSRTEFEA
HHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHH
MQADIAPDCDFSATGKLVLYASAAALAGARAQLELQRTMGSQQRIVAPDECIAIEPALAS
HHHCCCCCCCCCCCCCEEEEECHHHHHCCHHHHHHHHHCCCCCCEECCCCCEEECHHHHH
YRSQMAGAVYTPSECAADCLKVCAELMRSLEARGVRFLPGTDVHGFARNGHRIAAVHTSQ
HHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCEEEEEECCC
GDIEADAFVMALGSASHKLGRALGAYLPVYPLKGYSITVAVDPSPGAAPTVNVTDSARKV
CCCCCCCEEEECCCHHHHHHHHHHHHCCCEECCCCEEEEEECCCCCCCCEEEECHHHHHH
VFARIGSRLRVAGMAELVGHDAGIPATRIATLAAATRALFPHAGRLEELHPWTGMRPATP
HHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCCC
RGLPIIGRLARAPSNMLFNTGHGALGFTLAFGSALRVAEALASCAPTP
CCCHHHHHHHCCCCCCEEECCCCCEEHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MHVCVLGAGIVGLATAWQLERRGHQVTVIDRAAPGAGASGGNGAQLSYSYVQPLADPSIW
CEEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCEEEHHHHCCCCCHHHH
KQLPKLLLSPTSPLKLRPQLDPLQWRWGMAFLAACNAQTSRDTTAKLLALAASSRTEFEA
HHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHH
MQADIAPDCDFSATGKLVLYASAAALAGARAQLELQRTMGSQQRIVAPDECIAIEPALAS
HHHCCCCCCCCCCCCCEEEEECHHHHHCCHHHHHHHHHCCCCCCEECCCCCEEECHHHHH
YRSQMAGAVYTPSECAADCLKVCAELMRSLEARGVRFLPGTDVHGFARNGHRIAAVHTSQ
HHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCEEEEEECCC
GDIEADAFVMALGSASHKLGRALGAYLPVYPLKGYSITVAVDPSPGAAPTVNVTDSARKV
CCCCCCCEEEECCCHHHHHHHHHHHHCCCEECCCCEEEEEECCCCCCCCEEEECHHHHHH
VFARIGSRLRVAGMAELVGHDAGIPATRIATLAAATRALFPHAGRLEELHPWTGMRPATP
HHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCCC
RGLPIIGRLARAPSNMLFNTGHGALGFTLAFGSALRVAEALASCAPTP
CCCHHHHHHHCCCCCCEEECCCCCEEHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]