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The map label for this gene is yngI [H]

Identifier: 121610793

GI number: 121610793

Start: 4269345

End: 4270877

Strand: Direct

Name: yngI [H]

Synonym: Veis_3874

Alternate gene names: 121610793

Gene position: 4269345-4270877 (Clockwise)

Preceding gene: 121610792

Following gene: 121610794

Centisome position: 76.69

GC content: 67.71

Gene sequence:

>1533_bases
ATGAACCCCAACCTCTTGATCTACAACGGCGGCCTGATACTGCCAGGCACCCAGTTGCAGGCACAGGCCGACCGCGCCGC
CGCCGGCTTTGAACAGCTTGGACTGCGGGCTGGCGACACCGTCGCGGTGATGCTGCGCAACGATGTTCCCTATCTGGAGC
TGATGCTGGCCCTCAACCAGCTCGGCATTCACCTGGTGGCCGTGAACTGGCACTTCCAGGCCGAAGAGGCGGCCTACGTA
TTACGCGACAGCGGGGCGCAAGCTCTGGTCATCCATGCGGACCTGTGGCCCACGCTGGCACCTGCCGTGCCGCCGGACGT
GCGGGTGCTGGTGGTGCCCACACCACAGGAGATCATTGATGCCTACGGCCTGGCGCCGGCCCCTCTGCCCGACCAGGCGC
TGCAGTGGGCGCAATGGCGCGACGGCTTCGCTCCCAGCATGACGCCGCCAGTCCCATCGCCGGGCAGCATGCTCTACACC
TCCGGCACGACCGGCCGGCCCAAGGCCGTCATGCGCCTGCCCGGCACACCGCCCCAGCACGAAGGAACCATGCGCGTGCG
CGCAATGGCCAGCCACGCCCGTGAAGGCATGCGCACGGCCCTGGTCGGCCCGCTGTACCACGCCGGGCCCAATACCGCCG
CACGCGTGGCGCTACGGCTGGCCGAGCGCATCGTGGTGCTGCCGCGCTTCGATGCCGAGCAGTTGCTGCGCACAATCGAG
CAGCACCGTCTCACACACCTGTCGCTCGTGCCAGTGATGCTGGTGCGGCTGCTCAAGCTGCCGCGTGAAGTGCGCGAGCG
CTACGACCTGTCGTCCCTGGAGAACGTGACCCACGGCGGCTCGCCCTGCGCCCCGGAAGTGCGGCGCGCCATCATCGACT
GGTGGGGCCCCATCGTCAACGAGACCTATGGCTCCACGGAAATCGGCCTGGTGACCATGGTCAGCGCGCAGGAATGGCTG
CAGCGTCCGGGTACCGCTGGCCGCCCTTTCCCTGGAACGTCGGTCCGCATCCTTGGCCCCGAGGGCCAGATCCTGCCTCC
GGGAGAAACCGGCGAGATCTACGTCGACCCGGGCGACAACGCCCTGCCCTTCACCTATCGCAACGACGCGGCCGCCCGTC
AGGCCATCGAGCGCGACGGGCACATCACCAACGGCGATATGGGCTACCTCGACGCGGACGGCTACCTCTACGTCACCGAC
CGCAAACGCGACATGGTGATTTCCGGCGGGGTCAACATCTATCCGGCAGAAATCGAGCACGTGCTGGTGACGCATCCGGA
AGTCGCCGACTGCGCGGTATTCGGCATTCCCGATGCCGAGTACGGCGAAGCGCTGGCCGCCGCCGTGGTGCGATCGCCAG
GCAGCAGCTTGGACGCGGAGCAGGTGCGCGACTGGATGCGCCAACGCATGGCCGGCTACAAGGTGCCGCGGCACGTGGAA
TTCCATGCGGCGCTGCCGCGTGAAGGTATGGGCAAGGTGTTCAAGAACCAGTTGCGCGCCCCGCACTGGGAACGCGCCGG
CCGCCGCATCTGA

Upstream 100 bases:

>100_bases
AACCCGTACAAGCTGTGTTGCGCCGTTTGATGGCCGAAACCAGCATGCGTGTGGCTGACGTGCACGCACGCGAACTTCCC
CTCTCCACCCTCCATCCCGC

Downstream 100 bases:

>100_bases
GAAACGCCATGTATCCACCATCGACACATCTGCTGTTCGGCACCGAGCCCGACCAGTACACCTTCGTCTGGCCCGGCCAG
ACGGTATTCGGGGCCGGCAG

Product: AMP-dependent synthetase and ligase

Products: AMP; diphosphate; acyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MNPNLLIYNGGLILPGTQLQAQADRAAAGFEQLGLRAGDTVAVMLRNDVPYLELMLALNQLGIHLVAVNWHFQAEEAAYV
LRDSGAQALVIHADLWPTLAPAVPPDVRVLVVPTPQEIIDAYGLAPAPLPDQALQWAQWRDGFAPSMTPPVPSPGSMLYT
SGTTGRPKAVMRLPGTPPQHEGTMRVRAMASHAREGMRTALVGPLYHAGPNTAARVALRLAERIVVLPRFDAEQLLRTIE
QHRLTHLSLVPVMLVRLLKLPREVRERYDLSSLENVTHGGSPCAPEVRRAIIDWWGPIVNETYGSTEIGLVTMVSAQEWL
QRPGTAGRPFPGTSVRILGPEGQILPPGETGEIYVDPGDNALPFTYRNDAAARQAIERDGHITNGDMGYLDADGYLYVTD
RKRDMVISGGVNIYPAEIEHVLVTHPEVADCAVFGIPDAEYGEALAAAVVRSPGSSLDAEQVRDWMRQRMAGYKVPRHVE
FHAALPREGMGKVFKNQLRAPHWERAGRRI

Sequences:

>Translated_510_residues
MNPNLLIYNGGLILPGTQLQAQADRAAAGFEQLGLRAGDTVAVMLRNDVPYLELMLALNQLGIHLVAVNWHFQAEEAAYV
LRDSGAQALVIHADLWPTLAPAVPPDVRVLVVPTPQEIIDAYGLAPAPLPDQALQWAQWRDGFAPSMTPPVPSPGSMLYT
SGTTGRPKAVMRLPGTPPQHEGTMRVRAMASHAREGMRTALVGPLYHAGPNTAARVALRLAERIVVLPRFDAEQLLRTIE
QHRLTHLSLVPVMLVRLLKLPREVRERYDLSSLENVTHGGSPCAPEVRRAIIDWWGPIVNETYGSTEIGLVTMVSAQEWL
QRPGTAGRPFPGTSVRILGPEGQILPPGETGEIYVDPGDNALPFTYRNDAAARQAIERDGHITNGDMGYLDADGYLYVTD
RKRDMVISGGVNIYPAEIEHVLVTHPEVADCAVFGIPDAEYGEALAAAVVRSPGSSLDAEQVRDWMRQRMAGYKVPRHVE
FHAALPREGMGKVFKNQLRAPHWERAGRRI
>Mature_510_residues
MNPNLLIYNGGLILPGTQLQAQADRAAAGFEQLGLRAGDTVAVMLRNDVPYLELMLALNQLGIHLVAVNWHFQAEEAAYV
LRDSGAQALVIHADLWPTLAPAVPPDVRVLVVPTPQEIIDAYGLAPAPLPDQALQWAQWRDGFAPSMTPPVPSPGSMLYT
SGTTGRPKAVMRLPGTPPQHEGTMRVRAMASHAREGMRTALVGPLYHAGPNTAARVALRLAERIVVLPRFDAEQLLRTIE
QHRLTHLSLVPVMLVRLLKLPREVRERYDLSSLENVTHGGSPCAPEVRRAIIDWWGPIVNETYGSTEIGLVTMVSAQEWL
QRPGTAGRPFPGTSVRILGPEGQILPPGETGEIYVDPGDNALPFTYRNDAAARQAIERDGHITNGDMGYLDADGYLYVTD
RKRDMVISGGVNIYPAEIEHVLVTHPEVADCAVFGIPDAEYGEALAAAVVRSPGSSLDAEQVRDWMRQRMAGYKVPRHVE
FHAALPREGMGKVFKNQLRAPHWERAGRRI

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=529, Percent_Identity=28.1663516068053, Blast_Score=182, Evalue=7e-46,
Organism=Homo sapiens, GI42544132, Length=290, Percent_Identity=33.1034482758621, Blast_Score=145, Evalue=7e-35,
Organism=Homo sapiens, GI122937307, Length=490, Percent_Identity=27.5510204081633, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI115511026, Length=499, Percent_Identity=27.0541082164329, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI38505220, Length=367, Percent_Identity=29.4277929155313, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI157311624, Length=500, Percent_Identity=27.4, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI157311622, Length=500, Percent_Identity=27.4, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI58082049, Length=501, Percent_Identity=26.9461077844311, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI187761345, Length=502, Percent_Identity=25.4980079681275, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI187761343, Length=502, Percent_Identity=25.4980079681275, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI13325057, Length=552, Percent_Identity=27.1739130434783, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI40807357, Length=547, Percent_Identity=26.1425959780622, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI8923896, Length=541, Percent_Identity=24.2144177449168, Blast_Score=93, Evalue=6e-19,
Organism=Homo sapiens, GI13375727, Length=528, Percent_Identity=25.1893939393939, Blast_Score=93, Evalue=6e-19,
Organism=Homo sapiens, GI253970489, Length=371, Percent_Identity=27.2237196765499, Blast_Score=92, Evalue=8e-19,
Organism=Homo sapiens, GI13236579, Length=496, Percent_Identity=26.4112903225806, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI195947378, Length=416, Percent_Identity=25, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI28416953, Length=529, Percent_Identity=24.007561436673, Blast_Score=76, Evalue=9e-14,
Organism=Escherichia coli, GI1788107, Length=517, Percent_Identity=29.4003868471954, Blast_Score=178, Evalue=7e-46,
Organism=Escherichia coli, GI221142682, Length=513, Percent_Identity=26.9005847953216, Blast_Score=169, Evalue=5e-43,
Organism=Escherichia coli, GI145693145, Length=470, Percent_Identity=27.4468085106383, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI1786810, Length=515, Percent_Identity=26.7961165048544, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1790505, Length=522, Percent_Identity=24.3295019157088, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786529, Length=376, Percent_Identity=26.8617021276596, Blast_Score=76, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17531443, Length=501, Percent_Identity=26.1477045908184, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17559526, Length=502, Percent_Identity=26.4940239043825, Blast_Score=156, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17558820, Length=506, Percent_Identity=27.2727272727273, Blast_Score=149, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI71994690, Length=529, Percent_Identity=24.7637051039698, Blast_Score=147, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI71994703, Length=529, Percent_Identity=24.7637051039698, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI71994694, Length=529, Percent_Identity=24.7637051039698, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17560308, Length=530, Percent_Identity=28.3018867924528, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI32563687, Length=489, Percent_Identity=26.1758691206544, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17557194, Length=512, Percent_Identity=26.7578125, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17538037, Length=501, Percent_Identity=26.5469061876248, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17560140, Length=365, Percent_Identity=25.2054794520548, Blast_Score=105, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI71985884, Length=375, Percent_Identity=26.1333333333333, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71996755, Length=477, Percent_Identity=23.0607966457023, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI25141359, Length=378, Percent_Identity=24.3386243386243, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI71983001, Length=546, Percent_Identity=23.4432234432234, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71982997, Length=546, Percent_Identity=23.4432234432234, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319699, Length=485, Percent_Identity=23.5051546391753, Blast_Score=140, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6319264, Length=528, Percent_Identity=25, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24648253, Length=464, Percent_Identity=27.1551724137931, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24648255, Length=464, Percent_Identity=27.3706896551724, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI281365686, Length=464, Percent_Identity=26.5086206896552, Blast_Score=150, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24581924, Length=513, Percent_Identity=26.9005847953216, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI21355181, Length=499, Percent_Identity=26.0521042084168, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI21358303, Length=327, Percent_Identity=29.6636085626911, Blast_Score=139, Evalue=6e-33,
Organism=Drosophila melanogaster, GI21356441, Length=471, Percent_Identity=25.6900212314225, Blast_Score=136, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24648260, Length=352, Percent_Identity=30.1136363636364, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI18859661, Length=506, Percent_Identity=26.2845849802372, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI19922652, Length=496, Percent_Identity=26.4112903225806, Blast_Score=128, Evalue=8e-30,
Organism=Drosophila melanogaster, GI21356947, Length=490, Percent_Identity=26.530612244898, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24648257, Length=397, Percent_Identity=27.7078085642317, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24653035, Length=352, Percent_Identity=28.6931818181818, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI20130357, Length=346, Percent_Identity=28.6127167630058, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI161076582, Length=367, Percent_Identity=27.5204359673025, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24656500, Length=497, Percent_Identity=25.3521126760563, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI62472339, Length=528, Percent_Identity=23.6742424242424, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24667955, Length=528, Percent_Identity=23.6742424242424, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI20130353, Length=520, Percent_Identity=23.8461538461538, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI28573631, Length=520, Percent_Identity=23.8461538461538, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24667959, Length=395, Percent_Identity=25.0632911392405, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI21355225, Length=374, Percent_Identity=24.8663101604278, Blast_Score=82, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3

Molecular weight: Translated: 55911; Mature: 55911

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPNLLIYNGGLILPGTQLQAQADRAAAGFEQLGLRAGDTVAVMLRNDVPYLELMLALNQ
CCCCEEEEECCEEECCCCHHHHHHHHHHCHHHHCCCCCCEEEEEEECCCCHHHHHHHHHH
LGIHLVAVNWHFQAEEAAYVLRDSGAQALVIHADLWPTLAPAVPPDVRVLVVPTPQEIID
CCEEEEEEEEEEECCCCEEEEECCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCHHHHH
AYGLAPAPLPDQALQWAQWRDGFAPSMTPPVPSPGSMLYTSGTTGRPKAVMRLPGTPPQH
HHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEECCCCCCCC
EGTMRVRAMASHAREGMRTALVGPLYHAGPNTAARVALRLAERIVVLPRFDAEQLLRTIE
CCCHHHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHEEEECCCCHHHHHHHHH
QHRLTHLSLVPVMLVRLLKLPREVRERYDLSSLENVTHGGSPCAPEVRRAIIDWWGPIVN
HHHHHHHHHHHHHHHHHHHCHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
ETYGSTEIGLVTMVSAQEWLQRPGTAGRPFPGTSVRILGPEGQILPPGETGEIYVDPGDN
CCCCCCCCCEEEEECHHHHHHCCCCCCCCCCCCEEEEECCCCCEECCCCCCEEEECCCCC
ALPFTYRNDAAARQAIERDGHITNGDMGYLDADGYLYVTDRKRDMVISGGVNIYPAEIEH
CCCEEECCHHHHHHHHHHCCCCCCCCCCEEECCCEEEEECCCCEEEEECCCEEEECCCCE
VLVTHPEVADCAVFGIPDAEYGEALAAAVVRSPGSSLDAEQVRDWMRQRMAGYKVPRHVE
EEECCCCHHCEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEE
FHAALPREGMGKVFKNQLRAPHWERAGRRI
EEECCCCHHHHHHHHHHHCCCCHHHCCCCC
>Mature Secondary Structure
MNPNLLIYNGGLILPGTQLQAQADRAAAGFEQLGLRAGDTVAVMLRNDVPYLELMLALNQ
CCCCEEEEECCEEECCCCHHHHHHHHHHCHHHHCCCCCCEEEEEEECCCCHHHHHHHHHH
LGIHLVAVNWHFQAEEAAYVLRDSGAQALVIHADLWPTLAPAVPPDVRVLVVPTPQEIID
CCEEEEEEEEEEECCCCEEEEECCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCHHHHH
AYGLAPAPLPDQALQWAQWRDGFAPSMTPPVPSPGSMLYTSGTTGRPKAVMRLPGTPPQH
HHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEECCCCCCCC
EGTMRVRAMASHAREGMRTALVGPLYHAGPNTAARVALRLAERIVVLPRFDAEQLLRTIE
CCCHHHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHEEEECCCCHHHHHHHHH
QHRLTHLSLVPVMLVRLLKLPREVRERYDLSSLENVTHGGSPCAPEVRRAIIDWWGPIVN
HHHHHHHHHHHHHHHHHHHCHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
ETYGSTEIGLVTMVSAQEWLQRPGTAGRPFPGTSVRILGPEGQILPPGETGEIYVDPGDN
CCCCCCCCCEEEEECHHHHHHCCCCCCCCCCCCEEEEECCCCCEECCCCCCEEEECCCCC
ALPFTYRNDAAARQAIERDGHITNGDMGYLDADGYLYVTDRKRDMVISGGVNIYPAEIEH
CCCEEECCHHHHHHHHHHCCCCCCCCCCEEECCCEEEEECCCCEEEEECCCEEEECCCCE
VLVTHPEVADCAVFGIPDAEYGEALAAAVVRSPGSSLDAEQVRDWMRQRMAGYKVPRHVE
EEECCCCHHCEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEE
FHAALPREGMGKVFKNQLRAPHWERAGRRI
EEECCCCHHHHHHHHHHHCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; long-chain carboxylic acid; CoA

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]