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The map label for this gene is ogt [H]

Identifier: 121609517

GI number: 121609517

Start: 2893269

End: 2893811

Strand: Direct

Name: ogt [H]

Synonym: Veis_2561

Alternate gene names: 121609517

Gene position: 2893269-2893811 (Clockwise)

Preceding gene: 121609516

Following gene: 121609518

Centisome position: 51.97

GC content: 71.45

Gene sequence:

>543_bases
ATGCACTTCCATTCCCACACCGTGCAGACCCGCATCACCACGCCACTCGGGCCAGTGCGGCTGGCGGCCTCTCCGGCAGG
CTTGGCGGGCCTCTGGTTTGAGGGCCAGCGCCATGAACCGCTGCCCCTGCTGCACGGCGCCAGCGCCTGGAGCCATGCCC
CCGACCACCCCTTGCTACAGGCCGCCAGCGGCCAATTGCAGCAGTACCTGCGCGGCGCGCGCGCCGGCTTCGAGTTGCCG
CTGGACCTGTCGGGCGGCACCGCCTTTCAGCAAAGCGTCTGGCGCGCGCTGTTGACCATCGCCCGTGGCCAGACCATCAG
CTATGGCGCGCTCAGCCAACAGCTCGGCAGGCCCCGGGCGGTGCGCGCCGTCGGTGCTGCCGTGGGGCGCAACCCCCTGA
GCGTGGTCGTGCCCTGCCACCGCGTGCTGGGCACCGACGGCAGCCTGACCGGCTATGCCGGCGGCCTGGAGCGCAAGACC
GCCCTGCTGACCCTGGACGATGCGCCCCACCCACATCCAGGCCCGCACCAAGGAAACGCATGA

Upstream 100 bases:

>100_bases
CAAAGGCGAAGGCACTCCCGTGAGCCGCAGCGAAATGCACCACCCGCAGCGCAATCACGGCCCGCGCCTGGCCCCTGCCC
ATCCCCCTACTGGAACCCCT

Downstream 100 bases:

>100_bases
ACGGCCCGGGAACCCGCGCCATGTCGGCCTGGATCGGTGAATACACCCTGCTGGCAGCGTTGTGGGGCGCGTCCTTTTTG
TTCATGCGGCTGGGGGCGGC

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 180; Mature: 180

Protein sequence:

>180_residues
MHFHSHTVQTRITTPLGPVRLAASPAGLAGLWFEGQRHEPLPLLHGASAWSHAPDHPLLQAASGQLQQYLRGARAGFELP
LDLSGGTAFQQSVWRALLTIARGQTISYGALSQQLGRPRAVRAVGAAVGRNPLSVVVPCHRVLGTDGSLTGYAGGLERKT
ALLTLDDAPHPHPGPHQGNA

Sequences:

>Translated_180_residues
MHFHSHTVQTRITTPLGPVRLAASPAGLAGLWFEGQRHEPLPLLHGASAWSHAPDHPLLQAASGQLQQYLRGARAGFELP
LDLSGGTAFQQSVWRALLTIARGQTISYGALSQQLGRPRAVRAVGAAVGRNPLSVVVPCHRVLGTDGSLTGYAGGLERKT
ALLTLDDAPHPHPGPHQGNA
>Mature_180_residues
MHFHSHTVQTRITTPLGPVRLAASPAGLAGLWFEGQRHEPLPLLHGASAWSHAPDHPLLQAASGQLQQYLRGARAGFELP
LDLSGGTAFQQSVWRALLTIARGQTISYGALSQQLGRPRAVRAVGAAVGRNPLSVVVPCHRVLGTDGSLTGYAGGLERKT
ALLTLDDAPHPHPGPHQGNA

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=179, Percent_Identity=31.8435754189944, Blast_Score=72, Evalue=2e-13,
Organism=Escherichia coli, GI1787596, Length=101, Percent_Identity=53.4653465346535, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI1788542, Length=105, Percent_Identity=47.6190476190476, Blast_Score=99, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI115533070, Length=84, Percent_Identity=45.2380952380952, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI115533068, Length=85, Percent_Identity=44.7058823529412, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320001, Length=86, Percent_Identity=40.6976744186046, Blast_Score=87, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17137554, Length=78, Percent_Identity=48.7179487179487, Blast_Score=67, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 18991; Mature: 18991

Theoretical pI: Translated: 10.69; Mature: 10.69

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHFHSHTVQTRITTPLGPVRLAASPAGLAGLWFEGQRHEPLPLLHGASAWSHAPDHPLLQ
CCCCCCEEEEEECCCCCCEEEECCCCCCEEEEECCCCCCCCCEEECCCCCCCCCCCHHHH
AASGQLQQYLRGARAGFELPLDLSGGTAFQQSVWRALLTIARGQTISYGALSQQLGRPRA
HHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHH
VRAVGAAVGRNPLSVVVPCHRVLGTDGSLTGYAGGLERKTALLTLDDAPHPHPGPHQGNA
HHHHHHHHCCCCCEEEEEHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MHFHSHTVQTRITTPLGPVRLAASPAGLAGLWFEGQRHEPLPLLHGASAWSHAPDHPLLQ
CCCCCCEEEEEECCCCCCEEEECCCCCCEEEEECCCCCCCCCEEECCCCCCCCCCCHHHH
AASGQLQQYLRGARAGFELPLDLSGGTAFQQSVWRALLTIARGQTISYGALSQQLGRPRA
HHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHH
VRAVGAAVGRNPLSVVVPCHRVLGTDGSLTGYAGGLERKTALLTLDDAPHPHPGPHQGNA
HHHHHHHHCCCCCEEEEEHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]