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The map label for this gene is gcvA [H]

Identifier: 121609412

GI number: 121609412

Start: 2789494

End: 2790450

Strand: Direct

Name: gcvA [H]

Synonym: Veis_2456

Alternate gene names: 121609412

Gene position: 2789494-2790450 (Clockwise)

Preceding gene: 121609410

Following gene: 121609416

Centisome position: 50.11

GC content: 66.35

Gene sequence:

>957_bases
ATGCATCTACCGTGGCAAGCCATGCGCAAAAACCTTCACCACCTGAATGCACTGCGCAGCTTTGAGGCAGCGGCACGCCA
GTTGTCATTCACGAATGCGGCGCATGAGTTGTGCGTTACCCAAGCTGCGATCAGCCATCAGATCCGCGCGCTGGAACAAA
GCCTGGGCACGCAACTGTTCGAGCGCAGGCCCAGGCAGGTGCGGCTGACGGTGGCGGGCGAGCAGTTGCTGGCGGTGCTG
TCGGATAGTTTCGATGGCATCGAGCGGGTTCTGGCGAGTCTGGGCAACCCGCAGGTGAGCACTTTGCAATTGGCGGTGAC
GCCGACTTTTTCGAGCAAATGGCTGATGCCGCGCTTGCCGCGCTTCATCGATCGCCATCCCGACATCGAACTGCACCTGC
ACCATACGGCGCAGGCCGATGTGCTCACCCGGGGCCAGGTGGACATGGCCGTGATCTGGACCACCCGGCCGCCGCCACGG
CTGTGGGCCCGGCGCTTGTTCGGAACGGCGCTGACGCCGGTCTGCTCCCCCGCTCTGGAGCGCCCGGAGCATCCGCTGAG
CCAGCCCTCGGCCGTCTGCCACTACCCGCTGCTGCACGAAGATGGCCCTCAGGACTGGCAGCGCTGGCTGACCCATGTGG
GGCTATCGGTGCGCGCGACCCGGCGTGGCTTGGTGATCGACGACAGCAACGCCTTGCTGACAGCCGCCATGGCCGGCCGT
GGCATTGCGCTGGGGCGCTTGGCGCTGATCGCCGATGACCTGCGCTCGGGGCGGCTGGTGCGGCCGTTCGCACAGACCAT
CGAGGCCGAGGGCGCTTACTGGCTGATAGCACAGCCGAGCATGGTCGCGCAGCCACGGTTCCAGTCGTTGGCGCAATTCC
TGATGGATAGCCAGGGCGATCCGGACGCAGCGCCGGCTCAACCGGAGCCGGCCAGCCCGCCGCCGTCGACGACATAG

Upstream 100 bases:

>100_bases
GTGAGGAAAATTATTTCCTGCATGACTTGGTCTCATGGTCAAATTCATCCAGGCCCCCGGCAGCGCACAGCCTGCGCACA
GGCAGCGTACCGGTGGCCGC

Downstream 100 bases:

>100_bases
GCCGATCCGGTCACGAACGAGGACCGATCGCTGGCCAAAAACAGCACCACCTGGGCAATTTCCTCGGCCCGCCCCACACG
CATCAATGGCCGGGAATTGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MHLPWQAMRKNLHHLNALRSFEAAARQLSFTNAAHELCVTQAAISHQIRALEQSLGTQLFERRPRQVRLTVAGEQLLAVL
SDSFDGIERVLASLGNPQVSTLQLAVTPTFSSKWLMPRLPRFIDRHPDIELHLHHTAQADVLTRGQVDMAVIWTTRPPPR
LWARRLFGTALTPVCSPALERPEHPLSQPSAVCHYPLLHEDGPQDWQRWLTHVGLSVRATRRGLVIDDSNALLTAAMAGR
GIALGRLALIADDLRSGRLVRPFAQTIEAEGAYWLIAQPSMVAQPRFQSLAQFLMDSQGDPDAAPAQPEPASPPPSTT

Sequences:

>Translated_318_residues
MHLPWQAMRKNLHHLNALRSFEAAARQLSFTNAAHELCVTQAAISHQIRALEQSLGTQLFERRPRQVRLTVAGEQLLAVL
SDSFDGIERVLASLGNPQVSTLQLAVTPTFSSKWLMPRLPRFIDRHPDIELHLHHTAQADVLTRGQVDMAVIWTTRPPPR
LWARRLFGTALTPVCSPALERPEHPLSQPSAVCHYPLLHEDGPQDWQRWLTHVGLSVRATRRGLVIDDSNALLTAAMAGR
GIALGRLALIADDLRSGRLVRPFAQTIEAEGAYWLIAQPSMVAQPRFQSLAQFLMDSQGDPDAAPAQPEPASPPPSTT
>Mature_318_residues
MHLPWQAMRKNLHHLNALRSFEAAARQLSFTNAAHELCVTQAAISHQIRALEQSLGTQLFERRPRQVRLTVAGEQLLAVL
SDSFDGIERVLASLGNPQVSTLQLAVTPTFSSKWLMPRLPRFIDRHPDIELHLHHTAQADVLTRGQVDMAVIWTTRPPPR
LWARRLFGTALTPVCSPALERPEHPLSQPSAVCHYPLLHEDGPQDWQRWLTHVGLSVRATRRGLVIDDSNALLTAAMAGR
GIALGRLALIADDLRSGRLVRPFAQTIEAEGAYWLIAQPSMVAQPRFQSLAQFLMDSQGDPDAAPAQPEPASPPPSTT

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=289, Percent_Identity=34.2560553633218, Blast_Score=174, Evalue=7e-45,
Organism=Escherichia coli, GI1788706, Length=274, Percent_Identity=32.8467153284672, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1786448, Length=269, Percent_Identity=32.7137546468402, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI157672245, Length=180, Percent_Identity=35.5555555555556, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1787128, Length=270, Percent_Identity=29.6296296296296, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI145693193, Length=266, Percent_Identity=28.5714285714286, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI2367136, Length=278, Percent_Identity=30.2158273381295, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1790262, Length=186, Percent_Identity=29.5698924731183, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787530, Length=257, Percent_Identity=24.124513618677, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1787589, Length=235, Percent_Identity=28.936170212766, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1788887, Length=271, Percent_Identity=27.6752767527675, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1786401, Length=265, Percent_Identity=23.7735849056604, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI145693105, Length=124, Percent_Identity=32.258064516129, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788748, Length=169, Percent_Identity=26.6272189349112, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35237; Mature: 35237

Theoretical pI: Translated: 8.55; Mature: 8.55

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLPWQAMRKNLHHLNALRSFEAAARQLSFTNAAHELCVTQAAISHQIRALEQSLGTQLF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERRPRQVRLTVAGEQLLAVLSDSFDGIERVLASLGNPQVSTLQLAVTPTFSSKWLMPRLP
HCCCCEEEEEECHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCHHHHH
RFIDRHPDIELHLHHTAQADVLTRGQVDMAVIWTTRPPPRLWARRLFGTALTPVCSPALE
HHHHCCCCEEEEEECCCCCCCEECCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
RPEHPLSQPSAVCHYPLLHEDGPQDWQRWLTHVGLSVRATRRGLVIDDSNALLTAAMAGR
CCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHCCCEEEECCCCEEEECCCCEEEHHHCCC
GIALGRLALIADDLRSGRLVRPFAQTIEAEGAYWLIAQPSMVAQPRFQSLAQFLMDSQGD
CHHHHHHHHHHHHHHCCCEEHHHHHHHHCCCCEEEEECCCHHCCHHHHHHHHHHHCCCCC
PDAAPAQPEPASPPPSTT
CCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MHLPWQAMRKNLHHLNALRSFEAAARQLSFTNAAHELCVTQAAISHQIRALEQSLGTQLF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERRPRQVRLTVAGEQLLAVLSDSFDGIERVLASLGNPQVSTLQLAVTPTFSSKWLMPRLP
HCCCCEEEEEECHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCHHHHH
RFIDRHPDIELHLHHTAQADVLTRGQVDMAVIWTTRPPPRLWARRLFGTALTPVCSPALE
HHHHCCCCEEEEEECCCCCCCEECCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
RPEHPLSQPSAVCHYPLLHEDGPQDWQRWLTHVGLSVRATRRGLVIDDSNALLTAAMAGR
CCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHCCCEEEECCCCEEEECCCCEEEHHHCCC
GIALGRLALIADDLRSGRLVRPFAQTIEAEGAYWLIAQPSMVAQPRFQSLAQFLMDSQGD
CHHHHHHHHHHHHHHCCCEEHHHHHHHHCCCCEEEEECCCHHCCHHHHHHHHHHHCCCCC
PDAAPAQPEPASPPPSTT
CCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]