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The map label for this gene is stp [H]

Identifier: 121609410

GI number: 121609410

Start: 2787463

End: 2788995

Strand: Direct

Name: stp [H]

Synonym: Veis_2454

Alternate gene names: 121609410

Gene position: 2787463-2788995 (Clockwise)

Preceding gene: 121609408

Following gene: 121609412

Centisome position: 50.07

GC content: 51.53

Gene sequence:

>1533_bases
GTGAATAAAATTGAAAAAATCAACCCCGGTACATCCGGCAATGCATTATTGGCCGTCATCTTGCTGGCCATTTTTGTTGT
CCCCAGTTCAATTTCAGGTACGGCGCTTGCGTTGCCTGCGATCGGCATCGATCTTGGAGCGTCCATTGACAATTTACAGT
GGGTCGTCAATGCCTTCAATTTGATGTTCGCTTGCTTCACCCTCACCTGGGGGGCGTTGGCAGACAAAATTGGGCATAAA
CGTTGCTTCTTGATGGGGTCCGTGCTCTATGCCATTGCTTCAGTATGCTCTGCGATGGCAAGCGACCCATTGTTTCTCGA
TTTGATGAGAGGACTTGCAGGAATAGGGGCTGCTGCCGTTTTTTCTTGCGGAATCTCCATCCTGTCCATTCAATTCGAAG
GTGCGGCACGCATGCGTGCATTCGCATTTTTCGGAACGGTTGCCGGGTTGGGAGTAAGCCTCGGCCCTACATTGTCAGGA
ATCCTGTTGGGGTTCCATAGCTGGAATGCGATATTCTGGTTCCATGCCATTTCCTTGTCGATCGTCATTTTTGCCAGCTC
TCTCATTCCGGCAGACAAGGCCCGCGCCCAGGCAACGAAAGGGTTCGATACCATAGGGGCCACACTGTTCATTTTCCTGT
TGCTGGCCTTGATGATGGCAATAGTCCAGGCCCCGGTATGGGGTTGGGCCAACATGAAAATCATATCCTTGCTCATTGCC
AGTGCTTTACTGCTGGCTGCATTCATCAAATACGAAACCCATCGGCAGCAGCCCGTGCTCGACCTGTCTTTGTTGCTCAA
CGGCAAATTTGTAGGGATGGCATTGGTGACCGTTGCGGTTTCATTCGGGTTTGTCACGTTGCTGACCTATTTGCCCACCT
ATCTGATGACCGTTCTTCACCTGTCGGCCACGCAAGCGGGTCTGAGCATGATTCTATTGACGTCGCCGATGCTTGTTTGT
CCAATACTGGCGGGCAAATTGGCAACGAGGGGGGTCGATCCAGTCAATATCATCTACATCAGCCTTCTCAGCCTGCTTCT
TGGGGTCGCAAGTCTGGCGCTTATCAGCCATCCCCGGATTCCTGTGCTTCTGTTATCGTTGCCGCTGCTCTTGGTGGGCA
TAGGAATGGGCTTGTCTGCCGGCTTGGTGGATGGGCTGGCGCTGAAATTCGTGCCCGAAGAAAAAACCGGAATGGCAGCC
GGAGTTGTCAACACCTTCAGGCTGGGCAGCGAGGCCATTGCTGTCGCACTGTATGGCGCCTTTCTTTCCACTGTCCTGGA
GCTGGGCCTCAAAGATGTCTTGCACCCTTTTGCTGGCGAAACGGGGGTTGCAAAAACATGGATCGACAAACTTGCAGCAG
GCGACATCAACTTCCCTCTCCAGGCCGTTGCATCAGACAAGGCAGATGCGCTGAAAATCATTCTTGAAAATGGTTATAAC
GGTTCATTTCACTATACGCTTTGGGCTATCAGCATCATTCTTGTTGGATTGATGGTGGCAATTTACAAATTGATCCATTC
GGGCAAGGATTGA

Upstream 100 bases:

>100_bases
GCATGGCTTGATGGCCTGAGAAATTCAGCGACAGTTTGCATTATGGCCAGACTGTTCCGGAAGAGATGTTGCATCGCGCA
GCAATGAATGAAGTCTTTGT

Downstream 100 bases:

>100_bases
GTGCGAACGTGGTGGCATTGGGCCTGGCCGCCAGCGCCTTATCGTGCCCGTTTGCCGGCTGCGTCGTCTGCGCCCAACGA
CTCGAAGCGCGCCTTTTTGC

Product: major facilitator transporter

Products: NA

Alternate protein names: Spectinomycin tetracycline efflux pump [H]

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MNKIEKINPGTSGNALLAVILLAIFVVPSSISGTALALPAIGIDLGASIDNLQWVVNAFNLMFACFTLTWGALADKIGHK
RCFLMGSVLYAIASVCSAMASDPLFLDLMRGLAGIGAAAVFSCGISILSIQFEGAARMRAFAFFGTVAGLGVSLGPTLSG
ILLGFHSWNAIFWFHAISLSIVIFASSLIPADKARAQATKGFDTIGATLFIFLLLALMMAIVQAPVWGWANMKIISLLIA
SALLLAAFIKYETHRQQPVLDLSLLLNGKFVGMALVTVAVSFGFVTLLTYLPTYLMTVLHLSATQAGLSMILLTSPMLVC
PILAGKLATRGVDPVNIIYISLLSLLLGVASLALISHPRIPVLLLSLPLLLVGIGMGLSAGLVDGLALKFVPEEKTGMAA
GVVNTFRLGSEAIAVALYGAFLSTVLELGLKDVLHPFAGETGVAKTWIDKLAAGDINFPLQAVASDKADALKIILENGYN
GSFHYTLWAISIILVGLMVAIYKLIHSGKD

Sequences:

>Translated_510_residues
MNKIEKINPGTSGNALLAVILLAIFVVPSSISGTALALPAIGIDLGASIDNLQWVVNAFNLMFACFTLTWGALADKIGHK
RCFLMGSVLYAIASVCSAMASDPLFLDLMRGLAGIGAAAVFSCGISILSIQFEGAARMRAFAFFGTVAGLGVSLGPTLSG
ILLGFHSWNAIFWFHAISLSIVIFASSLIPADKARAQATKGFDTIGATLFIFLLLALMMAIVQAPVWGWANMKIISLLIA
SALLLAAFIKYETHRQQPVLDLSLLLNGKFVGMALVTVAVSFGFVTLLTYLPTYLMTVLHLSATQAGLSMILLTSPMLVC
PILAGKLATRGVDPVNIIYISLLSLLLGVASLALISHPRIPVLLLSLPLLLVGIGMGLSAGLVDGLALKFVPEEKTGMAA
GVVNTFRLGSEAIAVALYGAFLSTVLELGLKDVLHPFAGETGVAKTWIDKLAAGDINFPLQAVASDKADALKIILENGYN
GSFHYTLWAISIILVGLMVAIYKLIHSGKD
>Mature_510_residues
MNKIEKINPGTSGNALLAVILLAIFVVPSSISGTALALPAIGIDLGASIDNLQWVVNAFNLMFACFTLTWGALADKIGHK
RCFLMGSVLYAIASVCSAMASDPLFLDLMRGLAGIGAAAVFSCGISILSIQFEGAARMRAFAFFGTVAGLGVSLGPTLSG
ILLGFHSWNAIFWFHAISLSIVIFASSLIPADKARAQATKGFDTIGATLFIFLLLALMMAIVQAPVWGWANMKIISLLIA
SALLLAAFIKYETHRQQPVLDLSLLLNGKFVGMALVTVAVSFGFVTLLTYLPTYLMTVLHLSATQAGLSMILLTSPMLVC
PILAGKLATRGVDPVNIIYISLLSLLLGVASLALISHPRIPVLLLSLPLLLVGIGMGLSAGLVDGLALKFVPEEKTGMAA
GVVNTFRLGSEAIAVALYGAFLSTVLELGLKDVLHPFAGETGVAKTWIDKLAAGDINFPLQAVASDKADALKIILENGYN
GSFHYTLWAISIILVGLMVAIYKLIHSGKD

Specific function: Contributes to spectinomycin and tetracycline resistance [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87081983, Length=428, Percent_Identity=26.4018691588785, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1788710, Length=413, Percent_Identity=23.2445520581114, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1789042, Length=300, Percent_Identity=24.6666666666667, Blast_Score=70, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324954, Length=434, Percent_Identity=23.2718894009217, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323520, Length=429, Percent_Identity=26.3403263403263, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 53705; Mature: 53705

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKIEKINPGTSGNALLAVILLAIFVVPSSISGTALALPAIGIDLGASIDNLQWVVNAFN
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEHHHHCCCCCCCHHHHHHHHHHHH
LMFACFTLTWGALADKIGHKRCFLMGSVLYAIASVCSAMASDPLFLDLMRGLAGIGAAAV
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
FSCGISILSIQFEGAARMRAFAFFGTVAGLGVSLGPTLSGILLGFHSWNAIFWFHAISLS
HHCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHH
IVIFASSLIPADKARAQATKGFDTIGATLFIFLLLALMMAIVQAPVWGWANMKIISLLIA
HHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
SALLLAAFIKYETHRQQPVLDLSLLLNGKFVGMALVTVAVSFGFVTLLTYLPTYLMTVLH
HHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSATQAGLSMILLTSPMLVCPILAGKLATRGVDPVNIIYISLLSLLLGVASLALISHPRI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
PVLLLSLPLLLVGIGMGLSAGLVDGLALKFVPEEKTGMAAGVVNTFRLGSEAIAVALYGA
HHHHHHHHHHHHHHCCCHHHHHHHCHHEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHH
FLSTVLELGLKDVLHPFAGETGVAKTWIDKLAAGDINFPLQAVASDKADALKIILENGYN
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHCCCCC
GSFHYTLWAISIILVGLMVAIYKLIHSGKD
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNKIEKINPGTSGNALLAVILLAIFVVPSSISGTALALPAIGIDLGASIDNLQWVVNAFN
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEHHHHCCCCCCCHHHHHHHHHHHH
LMFACFTLTWGALADKIGHKRCFLMGSVLYAIASVCSAMASDPLFLDLMRGLAGIGAAAV
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
FSCGISILSIQFEGAARMRAFAFFGTVAGLGVSLGPTLSGILLGFHSWNAIFWFHAISLS
HHCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHH
IVIFASSLIPADKARAQATKGFDTIGATLFIFLLLALMMAIVQAPVWGWANMKIISLLIA
HHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
SALLLAAFIKYETHRQQPVLDLSLLLNGKFVGMALVTVAVSFGFVTLLTYLPTYLMTVLH
HHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSATQAGLSMILLTSPMLVCPILAGKLATRGVDPVNIIYISLLSLLLGVASLALISHPRI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
PVLLLSLPLLLVGIGMGLSAGLVDGLALKFVPEEKTGMAAGVVNTFRLGSEAIAVALYGA
HHHHHHHHHHHHHHCCCHHHHHHHCHHEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHH
FLSTVLELGLKDVLHPFAGETGVAKTWIDKLAAGDINFPLQAVASDKADALKIILENGYN
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHCCCCC
GSFHYTLWAISIILVGLMVAIYKLIHSGKD
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]