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The map label for this gene is proB

Identifier: 121638322

GI number: 121638322

Start: 2702287

End: 2703417

Strand: Reverse

Name: proB

Synonym: BCG_2459c

Alternate gene names: 121638322

Gene position: 2703417-2702287 (Counterclockwise)

Preceding gene: 121638323

Following gene: 121638320

Centisome position: 61.8

GC content: 70.56

Gene sequence:

>1131_bases
ATGAGAAGTCCGCATCGGGACGCAATCCGGACCGCGCGCGGCCTTGTCGTGAAGGTCGGGACCACGGCGCTTACCACACC
GTCCGGGATGTTCGATGCCGGCCGGCTGGCCGGACTGGCCGAGGCGGTCGAGCGGCGGATGAAGGCGGGTTCCGACGTCG
TCATCGTGTCTTCGGGCGCCATCGCCGCCGGCATCGAGCCGCTCGGGCTGTCCCGTCGTCCCAAAGATCTGGCGACCAAG
CAGGCGGCGGCCAGCGTCGGGCAGGTCGCGCTGGTGAACTCGTGGAGCGCGGCGTTCGCCCGCTACGGCCGCACGGTGGG
CCAGGTGCTGCTGACCGCGCACGACATTTCGATGCGGGTGCAGCACACCAACGCCCAACGCACGCTGGATCGGCTGCGCG
CGTTGCACGCGGTGGCGATTGTCAACGAGAACGACACCGTGGCCACCAACGAGATCCGGTTCGGTGACAACGATCGGCTG
TCTGCACTGGTGGCGCACCTGGTCGGCGCCGACGCTTTGGTGCTGCTGTCGGACATCGACGGCCTCTACGACTGCGACCC
GCGCAAAACCGCGGACGCGACGTTCATTCCGGAGGTGTCCGGGCCGGCGGATCTGGACGGTGTGGTCGCCGGCCGCAGTA
GCCACCTGGGTACTGGCGGCATGGCGTCCAAGGTGTCGGCGGCGCTGTTGGCCGCCGACGCCGGGGTGCCGGTACTGCTG
GCCCCCGCGGCCGACGCCGCGACCGCGCTCGCCGACGCGTCGGTGGGCACGGTGTTTGCGGCCCGGCCCGCGCGTCTGTC
GGCCCGGCGGTTCTGGGTGCGTTATGCCGCCGAAGCAACCGGCGCACTGACTCTCGACGCCGGTGCGGTGCGCGCTGTGG
TGCGACAACGCCGGTCACTGCTGGCGGCGGGTATCACCGCGGTGTCCGGCCGGTTTTGCGGCGGCGATGTGGTCGAACTG
CGTGCACCCGACGCGGCCATGGTAGCCCGCGGGGTGGTTGCCTACGACGCGTCCGAGCTGGCCACCATGGTGGGCCGGTC
CACCTCTGAGCTACCCGGCGAGCTGCGCCGCCCGGTGGTGCACGCCGACGATCTGGTCGCGGTGTCGGCGAAGCAAGCTA
AGCAAGTTTAG

Upstream 100 bases:

>100_bases
CCGGCCGGGGCACCGATCCGCGGCTGGACAGCAACAAGCGGGTGGGCGCGGCCGAGCGAAAGGCCGCTCGGAGTCGGCGT
CGCGAACACGGGGATGGCTG

Downstream 100 bases:

>100_bases
CTACCATATTGCTCCGAGATGCGGTGTACCCCAAATGATTTCGATCAACATGGCCGCTAGCTGCGCATTGCGGCCGAGCG
GGTATCCGAGGTGAACTAGT

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MRSPHRDAIRTARGLVVKVGTTALTTPSGMFDAGRLAGLAEAVERRMKAGSDVVIVSSGAIAAGIEPLGLSRRPKDLATK
QAAASVGQVALVNSWSAAFARYGRTVGQVLLTAHDISMRVQHTNAQRTLDRLRALHAVAIVNENDTVATNEIRFGDNDRL
SALVAHLVGADALVLLSDIDGLYDCDPRKTADATFIPEVSGPADLDGVVAGRSSHLGTGGMASKVSAALLAADAGVPVLL
APAADAATALADASVGTVFAARPARLSARRFWVRYAAEATGALTLDAGAVRAVVRQRRSLLAAGITAVSGRFCGGDVVEL
RAPDAAMVARGVVAYDASELATMVGRSTSELPGELRRPVVHADDLVAVSAKQAKQV

Sequences:

>Translated_376_residues
MRSPHRDAIRTARGLVVKVGTTALTTPSGMFDAGRLAGLAEAVERRMKAGSDVVIVSSGAIAAGIEPLGLSRRPKDLATK
QAAASVGQVALVNSWSAAFARYGRTVGQVLLTAHDISMRVQHTNAQRTLDRLRALHAVAIVNENDTVATNEIRFGDNDRL
SALVAHLVGADALVLLSDIDGLYDCDPRKTADATFIPEVSGPADLDGVVAGRSSHLGTGGMASKVSAALLAADAGVPVLL
APAADAATALADASVGTVFAARPARLSARRFWVRYAAEATGALTLDAGAVRAVVRQRRSLLAAGITAVSGRFCGGDVVEL
RAPDAAMVARGVVAYDASELATMVGRSTSELPGELRRPVVHADDLVAVSAKQAKQV
>Mature_376_residues
MRSPHRDAIRTARGLVVKVGTTALTTPSGMFDAGRLAGLAEAVERRMKAGSDVVIVSSGAIAAGIEPLGLSRRPKDLATK
QAAASVGQVALVNSWSAAFARYGRTVGQVLLTAHDISMRVQHTNAQRTLDRLRALHAVAIVNENDTVATNEIRFGDNDRL
SALVAHLVGADALVLLSDIDGLYDCDPRKTADATFIPEVSGPADLDGVVAGRSSHLGTGGMASKVSAALLAADAGVPVLL
APAADAATALADASVGTVFAARPARLSARRFWVRYAAEATGALTLDAGAVRAVVRQRRSLLAAGITAVSGRFCGGDVVEL
RAPDAAMVARGVVAYDASELATMVGRSTSELPGELRRPVVHADDLVAVSAKQAKQV

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI21361368, Length=299, Percent_Identity=31.1036789297659, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI62912457, Length=297, Percent_Identity=30.976430976431, Blast_Score=105, Evalue=6e-23,
Organism=Escherichia coli, GI1786437, Length=360, Percent_Identity=40.5555555555556, Blast_Score=224, Evalue=1e-59,
Organism=Caenorhabditis elegans, GI17569967, Length=274, Percent_Identity=34.6715328467153, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17569969, Length=274, Percent_Identity=34.6715328467153, Blast_Score=126, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6320506, Length=404, Percent_Identity=33.4158415841584, Blast_Score=187, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6321822, Length=356, Percent_Identity=33.9887640449438, Blast_Score=174, Evalue=2e-44,
Organism=Drosophila melanogaster, GI21357643, Length=289, Percent_Identity=35.2941176470588, Blast_Score=128, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_MYCBO (P59958)

Other databases:

- EMBL:   BX248342
- RefSeq:   NP_856113.1
- ProteinModelPortal:   P59958
- SMR:   P59958
- EnsemblBacteria:   EBMYCT00000014885
- GeneID:   1094249
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb2466c
- GeneTree:   EBGT00050000017499
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- ProtClustDB:   PRK05429
- BioCyc:   MBOV233413:MB2466C-MONOMER
- BRENDA:   2.7.2.11
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 38805; Mature: 38805

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSPHRDAIRTARGLVVKVGTTALTTPSGMFDAGRLAGLAEAVERRMKAGSDVVIVSSGA
CCCCHHHHHHHHCCEEEEECCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCC
IAAGIEPLGLSRRPKDLATKQAAASVGQVALVNSWSAAFARYGRTVGQVLLTAHDISMRV
CEECCCCCCCCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHEEEEEEEE
QHTNAQRTLDRLRALHAVAIVNENDTVATNEIRFGDNDRLSALVAHLVGADALVLLSDID
ECCCHHHHHHHHHHHHHEEEECCCCCEEECEEEECCCHHHHHHHHHHHCCCCEEEEECCC
GLYDCDPRKTADATFIPEVSGPADLDGVVAGRSSHLGTGGMASKVSAALLAADAGVPVLL
CCCCCCCCCCCCCEECCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEE
APAADAATALADASVGTVFAARPARLSARRFWVRYAAEATGALTLDAGAVRAVVRQRRSL
ECCCHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHH
LAAGITAVSGRFCGGDVVELRAPDAAMVARGVVAYDASELATMVGRSTSELPGELRRPVV
HHHHHHHHCCCCCCCCEEEEECCCHHHHHCCHHHCCHHHHHHHHCCCCCCCCHHHHCCEE
HADDLVAVSAKQAKQV
CCCCEEEECHHHHCCC
>Mature Secondary Structure
MRSPHRDAIRTARGLVVKVGTTALTTPSGMFDAGRLAGLAEAVERRMKAGSDVVIVSSGA
CCCCHHHHHHHHCCEEEEECCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCC
IAAGIEPLGLSRRPKDLATKQAAASVGQVALVNSWSAAFARYGRTVGQVLLTAHDISMRV
CEECCCCCCCCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHEEEEEEEE
QHTNAQRTLDRLRALHAVAIVNENDTVATNEIRFGDNDRLSALVAHLVGADALVLLSDID
ECCCHHHHHHHHHHHHHEEEECCCCCEEECEEEECCCHHHHHHHHHHHCCCCEEEEECCC
GLYDCDPRKTADATFIPEVSGPADLDGVVAGRSSHLGTGGMASKVSAALLAADAGVPVLL
CCCCCCCCCCCCCEECCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEE
APAADAATALADASVGTVFAARPARLSARRFWVRYAAEATGALTLDAGAVRAVVRQRRSL
ECCCHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHH
LAAGITAVSGRFCGGDVVELRAPDAAMVARGVVAYDASELATMVGRSTSELPGELRRPVV
HHHHHHHHCCCCCCCCEEEEECCCHHHHHCCHHHCCHHHHHHHHCCCCCCCCHHHHCCEE
HADDLVAVSAKQAKQV
CCCCEEEECHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972