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The map label for this gene is nadE

Identifier: 121638320

GI number: 121638320

Start: 2699962

End: 2702001

Strand: Reverse

Name: nadE

Synonym: BCG_2457c

Alternate gene names: 121638320

Gene position: 2702001-2699962 (Counterclockwise)

Preceding gene: 121638322

Following gene: 121638317

Centisome position: 61.77

GC content: 63.73

Gene sequence:

>2040_bases
ATGAACTTTTACTCCGCCTACCAGCACGGGTTCGTGCGCGTTGCCGCCTGCACTCACCACACCACCATCGGTGACCCGGC
GGCCAACGCCGCGTCGGTATTGGACATGGCCCGTGCGTGCCACGACGATGGCGCAGCGTTGGCGGTCTTTCCTGAGCTGA
CGCTGTCGGGCTACTCCATCGAGGACGTACTACTGCAGGACTCTCTGCTCGATGCCGTCGAGGACGCGCTGCTCGACCTG
GTGACCGAATCCGCCGACCTGTTACCTGTACTGGTGGTCGGGGCTCCGCTGCGGCATCGACACCGCATCTACAACACCGC
GGTCGTCATTCACCGCGGCGCCGTGCTCGGCGTGGTGCCCAAGTCGTATCTACCCACCTATCGCGAGTTCTACGAGCGGC
GCCAGATGGCGCCCGGAGACGGGGAGCGGGGCACGATCCGCATCGGTGGCGCCGACGTGGCCTTCGGCACGGACCTGTTG
TTCGCCGCGTCAGATCTACCCGGCTTTGTGTTGCATGTGGAGATCTGCGAGGACATGTTTGTGCCGATGCCGCCCAGCGC
CGAGGCGGCCCTGGCGGGCGCGACGGTGCTGGCGAATCTGTCCGGCAGCCCGATCACCATCGGCCGTGCCGAGGACCGCC
GGCTGCTTGCGCGCTCGGCGTCGGCGCGGTGTCTGGCTGCCTATGTCTATGCCGCCGCGGGGGAGGGGGAGTCAACGACG
GACCTGGCCTGGGACGGTCAGACGATGATCTGGGAGAATGGCGCACTGCTCGCGGAGTCCGAACGTTTCCCCAAAGGAGT
GCGCCGCAGTGTCGCCGACGTTGACACCGAGTTGCTTCGGTCGGAGCGGCTGCGGATGGGCACGTTCGACGACAACCGGC
GTCACCACCGGGAGTTAACGGAATCGTTCCGGCGCATCGACTTCGCACTCGACCCACCGGCAGGCGACATCGGACTGCTG
CGCGAGGTCGAGCGGTTCCCGTTCGTTCCGGCCGATCCGCAACGATTGCAACAGGATTGCTACGAGGCCTACAACATCCA
GGTGTCTGGACTCGAGCAACGGTTGCGGGCGCTGGACTATCCGAAGGTCGTTATCGGTGTGTCCGGGGGATTGGACTCGA
CGCACGCGCTGATCGTCGCGACCCATGCCATGGACCGCGAGGGCCGGCCGCGCAGCGACATTCTGGCGTTTGCGTTGCCC
GGATTCGCCACCGGGGAGCACACTAAGAACAACGCGATCAAGCTGGCACGTGCGCTGGGGGTTACCTTCTCCGAAATCGA
TATCGGCGACACCGCTCGGTTGATGCTGCACACAATCGGCCATCCGTATTCGGTTGGCGAAAAAGTGTACGACGTCACCT
TCGAGAACGTCCAGGCCGGGTTGCGCACCGACTATCTTTTCCGTATCGCCAACCAGCGCGGGGGAATCGTACTGGGCACC
GGGGACCTGTCGGAGCTGGCACTGGGTTGGTCGACATACGGTGTCGGCGACCAGATGTCGCACTACAACGTCAACGCCGG
TGTGCCCAAGACGCTGATCCAGCACCTGATCCGGTGGGTCATTTCGGCGGGTGAGTTCGGTGAGAAGGTGGGTGAGGTAT
TGCAGTCGGTGCTCGACACCGAGATCACCCCCGAACTCATTCCGACCGGCGAGGAGGAGCTGCAGAGCAGCGAGGCCAAG
GTCGGACCTTTCGCCCTACAGGACTTTTCGCTTTTTCAGGTACTGCGCTACGGATTTCGCCCGTCGAAGATTGCGTTTTT
GGCCTGGCATGCGTGGAACGATGCGGAGCGGGGCAACTGGCCGCCCGGCTTCCCAAAGAGCGAACGCCCGTCCTATTCAT
TGGCCGAAATCCGGCATTGGCTGCAGATTTTCGTCCAGCGGTTTTATTCGTTTAGCCAGTTCAAGCGTTCGGCATTGCCC
AACGGCCCCAAGGTGTCCCACGGGGGCGCGTTGTCGCCGCGTGGGGATTGGCGGGCCCCGTCGGATATGTCAGCGCGAAT
CTGGCTCGATCAGATCGACCGTGAGGTGCCCAAGGGCTAG

Upstream 100 bases:

>100_bases
CGCCATCTGCCAGCAGCGCGGGGGATTCCACGGTGTCGAGCGTGGATACTCGGCGGGTGATTCGGGCGTTCTGACGTCGC
TGGGCGACAATGAAAGGACG

Downstream 100 bases:

>100_bases
CGGATCAACCCCACACCCGGGACGAACCGGCCGACGCTGGCGGTGTAGCCGCGGTAGACGGCACTGTGCGTCCGCAACAG
GTAGGGTTCTTCGACGCGGC

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing]

Number of amino acids: Translated: 679; Mature: 679

Protein sequence:

>679_residues
MNFYSAYQHGFVRVAACTHHTTIGDPAANAASVLDMARACHDDGAALAVFPELTLSGYSIEDVLLQDSLLDAVEDALLDL
VTESADLLPVLVVGAPLRHRHRIYNTAVVIHRGAVLGVVPKSYLPTYREFYERRQMAPGDGERGTIRIGGADVAFGTDLL
FAASDLPGFVLHVEICEDMFVPMPPSAEAALAGATVLANLSGSPITIGRAEDRRLLARSASARCLAAYVYAAAGEGESTT
DLAWDGQTMIWENGALLAESERFPKGVRRSVADVDTELLRSERLRMGTFDDNRRHHRELTESFRRIDFALDPPAGDIGLL
REVERFPFVPADPQRLQQDCYEAYNIQVSGLEQRLRALDYPKVVIGVSGGLDSTHALIVATHAMDREGRPRSDILAFALP
GFATGEHTKNNAIKLARALGVTFSEIDIGDTARLMLHTIGHPYSVGEKVYDVTFENVQAGLRTDYLFRIANQRGGIVLGT
GDLSELALGWSTYGVGDQMSHYNVNAGVPKTLIQHLIRWVISAGEFGEKVGEVLQSVLDTEITPELIPTGEEELQSSEAK
VGPFALQDFSLFQVLRYGFRPSKIAFLAWHAWNDAERGNWPPGFPKSERPSYSLAEIRHWLQIFVQRFYSFSQFKRSALP
NGPKVSHGGALSPRGDWRAPSDMSARIWLDQIDREVPKG

Sequences:

>Translated_679_residues
MNFYSAYQHGFVRVAACTHHTTIGDPAANAASVLDMARACHDDGAALAVFPELTLSGYSIEDVLLQDSLLDAVEDALLDL
VTESADLLPVLVVGAPLRHRHRIYNTAVVIHRGAVLGVVPKSYLPTYREFYERRQMAPGDGERGTIRIGGADVAFGTDLL
FAASDLPGFVLHVEICEDMFVPMPPSAEAALAGATVLANLSGSPITIGRAEDRRLLARSASARCLAAYVYAAAGEGESTT
DLAWDGQTMIWENGALLAESERFPKGVRRSVADVDTELLRSERLRMGTFDDNRRHHRELTESFRRIDFALDPPAGDIGLL
REVERFPFVPADPQRLQQDCYEAYNIQVSGLEQRLRALDYPKVVIGVSGGLDSTHALIVATHAMDREGRPRSDILAFALP
GFATGEHTKNNAIKLARALGVTFSEIDIGDTARLMLHTIGHPYSVGEKVYDVTFENVQAGLRTDYLFRIANQRGGIVLGT
GDLSELALGWSTYGVGDQMSHYNVNAGVPKTLIQHLIRWVISAGEFGEKVGEVLQSVLDTEITPELIPTGEEELQSSEAK
VGPFALQDFSLFQVLRYGFRPSKIAFLAWHAWNDAERGNWPPGFPKSERPSYSLAEIRHWLQIFVQRFYSFSQFKRSALP
NGPKVSHGGALSPRGDWRAPSDMSARIWLDQIDREVPKG
>Mature_679_residues
MNFYSAYQHGFVRVAACTHHTTIGDPAANAASVLDMARACHDDGAALAVFPELTLSGYSIEDVLLQDSLLDAVEDALLDL
VTESADLLPVLVVGAPLRHRHRIYNTAVVIHRGAVLGVVPKSYLPTYREFYERRQMAPGDGERGTIRIGGADVAFGTDLL
FAASDLPGFVLHVEICEDMFVPMPPSAEAALAGATVLANLSGSPITIGRAEDRRLLARSASARCLAAYVYAAAGEGESTT
DLAWDGQTMIWENGALLAESERFPKGVRRSVADVDTELLRSERLRMGTFDDNRRHHRELTESFRRIDFALDPPAGDIGLL
REVERFPFVPADPQRLQQDCYEAYNIQVSGLEQRLRALDYPKVVIGVSGGLDSTHALIVATHAMDREGRPRSDILAFALP
GFATGEHTKNNAIKLARALGVTFSEIDIGDTARLMLHTIGHPYSVGEKVYDVTFENVQAGLRTDYLFRIANQRGGIVLGT
GDLSELALGWSTYGVGDQMSHYNVNAGVPKTLIQHLIRWVISAGEFGEKVGEVLQSVLDTEITPELIPTGEEELQSSEAK
VGPFALQDFSLFQVLRYGFRPSKIAFLAWHAWNDAERGNWPPGFPKSERPSYSLAEIRHWLQIFVQRFYSFSQFKRSALP
NGPKVSHGGALSPRGDWRAPSDMSARIWLDQIDREVPKG

Specific function: Can use both glutamine or ammonia as a nitrogen source

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain

Homologues:

Organism=Homo sapiens, GI41393551, Length=625, Percent_Identity=23.2, Blast_Score=83, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17538756, Length=267, Percent_Identity=26.2172284644195, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6321865, Length=654, Percent_Identity=23.7003058103976, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24641841, Length=634, Percent_Identity=23.6593059936909, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24641843, Length=634, Percent_Identity=23.6593059936909, Blast_Score=84, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NADE_MYCBO (P0A5L7)

Other databases:

- EMBL:   BX248342
- RefSeq:   NP_856111.1
- ProteinModelPortal:   P0A5L7
- EnsemblBacteria:   EBMYCT00000017874
- GeneID:   1094244
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb2464c
- GeneTree:   EBGT00050000017830
- HOGENOM:   HBG732101
- OMA:   DGPKVGS
- ProtClustDB:   PRK02628
- BioCyc:   MBOV233413:MB2464C-MONOMER
- BRENDA:   6.3.5.1
- HAMAP:   MF_00193
- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.60.110.10
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF006630
- TIGRFAMs:   TIGR00552

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase; SSF56317 Ntlse/CNhydtse

EC number: =6.3.5.1

Molecular weight: Translated: 74683; Mature: 74683

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS50263 CN_HYDROLASE; PS00591 GLYCOSYL_HYDROL_F10

Important sites: ACT_SITE 368-368

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFYSAYQHGFVRVAACTHHTTIGDPAANAASVLDMARACHDDGAALAVFPELTLSGYSI
CCCHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCEECCCCH
EDVLLQDSLLDAVEDALLDLVTESADLLPVLVVGAPLRHRHRIYNTAVVIHRGAVLGVVP
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHEEEEEECCCEEEECC
KSYLPTYREFYERRQMAPGDGERGTIRIGGADVAFGTDLLFAASDLPGFVLHVEICEDMF
HHHCCHHHHHHHHHCCCCCCCCCCEEEECCCCEECCCHHHEEECCCCCEEEEEEEHHHCC
VPMPPSAEAALAGATVLANLSGSPITIGRAEDRRLLARSASARCLAAYVYAAAGEGESTT
CCCCCCCHHHHHHHHHEEECCCCCEEECCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCC
DLAWDGQTMIWENGALLAESERFPKGVRRSVADVDTELLRSERLRMGTFDDNRRHHRELT
EEEECCCEEEEECCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
ESFRRIDFALDPPAGDIGLLREVERFPFVPADPQRLQQDCYEAYNIQVSGLEQRLRALDY
HHHHHCCEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEECHHHHHHHCCC
PKVVIGVSGGLDSTHALIVATHAMDREGRPRSDILAFALPGFATGEHTKNNAIKLARALG
CEEEEEECCCCCCCCEEEEEEEHHCCCCCCHHHEEEEECCCCCCCCCCCCHHHHHHHHHC
VTFSEIDIGDTARLMLHTIGHPYSVGEKVYDVTFENVQAGLRTDYLFRIANQRGGIVLGT
CEEEEECCCCHHHHHHHHCCCCHHHCCHHEEEEHHHHHHCCCHHHHHEECCCCCCEEEEC
GDLSELALGWSTYGVGDQMSHYNVNAGVPKTLIQHLIRWVISAGEFGEKVGEVLQSVLDT
CCHHHHHHCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
EITPELIPTGEEELQSSEAKVGPFALQDFSLFQVLRYGFRPSKIAFLAWHAWNDAERGNW
CCCCCCCCCCHHHHHCCCCCCCCEEHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCC
PPGFPKSERPSYSLAEIRHWLQIFVQRFYSFSQFKRSALPNGPKVSHGGALSPRGDWRAP
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
SDMSARIWLDQIDREVPKG
CCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNFYSAYQHGFVRVAACTHHTTIGDPAANAASVLDMARACHDDGAALAVFPELTLSGYSI
CCCHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCEECCCCH
EDVLLQDSLLDAVEDALLDLVTESADLLPVLVVGAPLRHRHRIYNTAVVIHRGAVLGVVP
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHEEEEEECCCEEEECC
KSYLPTYREFYERRQMAPGDGERGTIRIGGADVAFGTDLLFAASDLPGFVLHVEICEDMF
HHHCCHHHHHHHHHCCCCCCCCCCEEEECCCCEECCCHHHEEECCCCCEEEEEEEHHHCC
VPMPPSAEAALAGATVLANLSGSPITIGRAEDRRLLARSASARCLAAYVYAAAGEGESTT
CCCCCCCHHHHHHHHHEEECCCCCEEECCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCC
DLAWDGQTMIWENGALLAESERFPKGVRRSVADVDTELLRSERLRMGTFDDNRRHHRELT
EEEECCCEEEEECCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
ESFRRIDFALDPPAGDIGLLREVERFPFVPADPQRLQQDCYEAYNIQVSGLEQRLRALDY
HHHHHCCEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEECHHHHHHHCCC
PKVVIGVSGGLDSTHALIVATHAMDREGRPRSDILAFALPGFATGEHTKNNAIKLARALG
CEEEEEECCCCCCCCEEEEEEEHHCCCCCCHHHEEEEECCCCCCCCCCCCHHHHHHHHHC
VTFSEIDIGDTARLMLHTIGHPYSVGEKVYDVTFENVQAGLRTDYLFRIANQRGGIVLGT
CEEEEECCCCHHHHHHHHCCCCHHHCCHHEEEEHHHHHHCCCHHHHHEECCCCCCEEEEC
GDLSELALGWSTYGVGDQMSHYNVNAGVPKTLIQHLIRWVISAGEFGEKVGEVLQSVLDT
CCHHHHHHCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
EITPELIPTGEEELQSSEAKVGPFALQDFSLFQVLRYGFRPSKIAFLAWHAWNDAERGNW
CCCCCCCCCCHHHHHCCCCCCCCEEHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCC
PPGFPKSERPSYSLAEIRHWLQIFVQRFYSFSQFKRSALPNGPKVSHGGALSPRGDWRAP
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
SDMSARIWLDQIDREVPKG
CCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972