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The map label for this gene is ksgA

Identifier: 121636939

GI number: 121636939

Start: 1159641

End: 1160594

Strand: Direct

Name: ksgA

Synonym: BCG_1067

Alternate gene names: 121636939

Gene position: 1159641-1160594 (Clockwise)

Preceding gene: 121636938

Following gene: 121636940

Centisome position: 26.51

GC content: 66.88

Gene sequence:

>954_bases
ATGTGCTGTACGAGCGGGTGCGCGCTGACCATCCGGCTGCTCGGGCGCACTGAGATCAGGCGGCTGGCCAAAGAGCTCGA
CTTTCGGCCGCGCAAATCTCTCGGACAGAACTTCGTGCACGACGCCAACACGGTGCGACGGGTGGTTGCCGCCTCCGGGG
TCAGCCGTTCCGACCTGGTTTTGGAGGTCGGGCCGGGCCTGGGATCGCTGACCCTGGCACTGCTCGACCGCGGCGCGACC
GTCACCGCGGTCGAGATCGATCCACTACTGGCTTCTCGGCTGCAACAGACCGTGGCGGAGCACTCGCACAGCGAGGTTCA
CCGACTAACGGTGGTCAATCGCGACGTCCTGGCCCTGCGCCGGGAGGATCTAGCCGCGGCGCCGACCGCGGTGGTTGCCA
ATCTGCCGTACAACGTAGCGGTACCGGCGTTGTTGCATCTGCTTGTCGAGTTCCCGTCGATCCGTGTCGTGACGGTGATG
GTGCAGGCCGAGGTCGCCGAACGGCTCGCCGCCGAGCCGGGCAGCAAAGAGTACGGCGTGCCCAGCGTTAAGCTGCGCTT
CTTCGGGCGGGTTCGCCGCTGCGGCATGGTGTCGCCGACCGTTTTCTGGCCCATTCCGCGTGTCTATTCCGGGCTGGTAC
GCATCGATCGATATGAGACCTCGCCCTGGCCCACCGACGACGCTTTTCGACGGCGGGTATTCGAACTCGTGGACATCGCA
TTCGCGCAGCGGCGCAAGACTTCTCGCAACGCGTTTGTGCAGTGGGCGGGCTCGGGAAGCGAGTCGGCGAATCGATTGTT
GGCGGCCAGCATCGACCCCGCCCGTCGCGGTGAGACGCTGTCCATCGACGACTTCGTGCGGCTGCTGCGACGGTCCGGCG
GCTCCGACGAGGCCACCAGCACCGGCCGGGACGCCAGGGCGCCGGACATTTCGGGGCACGCGTCGGCGAGCTGA

Upstream 100 bases:

>100_bases
GGCTGCGGTATGCACCCCGCGCTGACCTCGCCACCCGCGAAGAGCAGATCGCCGTTGCCGAGGTGACCCGACTGCGTCAA
GGTTGGGGCGCCTGGCCGGT

Downstream 100 bases:

>100_bases
CGGGGCGCCGCCGCGTGTGGTCGGCGCGTCACAGCGATAGTCTGCTGCGGTGTCCGCATCTGACGGCAACACCGCTGAAT
TGTGGGTGCCCACCGGGTCG

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MCCTSGCALTIRLLGRTEIRRLAKELDFRPRKSLGQNFVHDANTVRRVVAASGVSRSDLVLEVGPGLGSLTLALLDRGAT
VTAVEIDPLLASRLQQTVAEHSHSEVHRLTVVNRDVLALRREDLAAAPTAVVANLPYNVAVPALLHLLVEFPSIRVVTVM
VQAEVAERLAAEPGSKEYGVPSVKLRFFGRVRRCGMVSPTVFWPIPRVYSGLVRIDRYETSPWPTDDAFRRRVFELVDIA
FAQRRKTSRNAFVQWAGSGSESANRLLAASIDPARRGETLSIDDFVRLLRRSGGSDEATSTGRDARAPDISGHASAS

Sequences:

>Translated_317_residues
MCCTSGCALTIRLLGRTEIRRLAKELDFRPRKSLGQNFVHDANTVRRVVAASGVSRSDLVLEVGPGLGSLTLALLDRGAT
VTAVEIDPLLASRLQQTVAEHSHSEVHRLTVVNRDVLALRREDLAAAPTAVVANLPYNVAVPALLHLLVEFPSIRVVTVM
VQAEVAERLAAEPGSKEYGVPSVKLRFFGRVRRCGMVSPTVFWPIPRVYSGLVRIDRYETSPWPTDDAFRRRVFELVDIA
FAQRRKTSRNAFVQWAGSGSESANRLLAASIDPARRGETLSIDDFVRLLRRSGGSDEATSTGRDARAPDISGHASAS
>Mature_317_residues
MCCTSGCALTIRLLGRTEIRRLAKELDFRPRKSLGQNFVHDANTVRRVVAASGVSRSDLVLEVGPGLGSLTLALLDRGAT
VTAVEIDPLLASRLQQTVAEHSHSEVHRLTVVNRDVLALRREDLAAAPTAVVANLPYNVAVPALLHLLVEFPSIRVVTVM
VQAEVAERLAAEPGSKEYGVPSVKLRFFGRVRRCGMVSPTVFWPIPRVYSGLVRIDRYETSPWPTDDAFRRRVFELVDIA
FAQRRKTSRNAFVQWAGSGSESANRLLAASIDPARRGETLSIDDFVRLLRRSGGSDEATSTGRDARAPDISGHASAS

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=284, Percent_Identity=30.2816901408451, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI156415992, Length=308, Percent_Identity=30.1948051948052, Blast_Score=85, Evalue=8e-17,
Organism=Escherichia coli, GI1786236, Length=262, Percent_Identity=33.969465648855, Blast_Score=136, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI25146882, Length=181, Percent_Identity=35.3591160220994, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI25141369, Length=250, Percent_Identity=28, Blast_Score=77, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6324989, Length=191, Percent_Identity=30.3664921465969, Blast_Score=83, Evalue=7e-17,
Organism=Drosophila melanogaster, GI21358017, Length=280, Percent_Identity=29.6428571428571, Blast_Score=108, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_MYCBO (P66661)

Other databases:

- EMBL:   BX248337
- RefSeq:   NP_854694.1
- ProteinModelPortal:   P66661
- SMR:   P66661
- EnsemblBacteria:   EBMYCT00000015153
- GeneID:   1093354
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb1037
- GeneTree:   EBGT00050000014608
- HOGENOM:   HBG319664
- OMA:   RAENLTP
- ProtClustDB:   PRK00274
- BioCyc:   MBOV233413:MB1037-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 34646; Mature: 34646

Theoretical pI: Translated: 10.34; Mature: 10.34

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 37-37 BINDING 39-39 BINDING 64-64 BINDING 85-85 BINDING 115-115 BINDING 134-134

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCCTSGCALTIRLLGRTEIRRLAKELDFRPRKSLGQNFVHDANTVRRVVAASGVSRSDLV
CCCCCCCEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
LEVGPGLGSLTLALLDRGATVTAVEIDPLLASRLQQTVAEHSHSEVHRLTVVNRDVLALR
EEECCCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHH
REDLAAAPTAVVANLPYNVAVPALLHLLVEFPSIRVVTVMVQAEVAERLAAEPGSKEYGV
HHHHHHCCHHHEECCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCCCCCCCC
PSVKLRFFGRVRRCGMVSPTVFWPIPRVYSGLVRIDRYETSPWPTDDAFRRRVFELVDIA
CHHHHHHHHHHHHCCCCCCHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
FAQRRKTSRNAFVQWAGSGSESANRLLAASIDPARRGETLSIDDFVRLLRRSGGSDEATS
HHHHHHCCCCCEEEECCCCCCHHCEEEEECCCHHHCCCEECHHHHHHHHHHCCCCCCCCC
TGRDARAPDISGHASAS
CCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MCCTSGCALTIRLLGRTEIRRLAKELDFRPRKSLGQNFVHDANTVRRVVAASGVSRSDLV
CCCCCCCEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
LEVGPGLGSLTLALLDRGATVTAVEIDPLLASRLQQTVAEHSHSEVHRLTVVNRDVLALR
EEECCCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHH
REDLAAAPTAVVANLPYNVAVPALLHLLVEFPSIRVVTVMVQAEVAERLAAEPGSKEYGV
HHHHHHCCHHHEECCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCCCCCCCC
PSVKLRFFGRVRRCGMVSPTVFWPIPRVYSGLVRIDRYETSPWPTDDAFRRRVFELVDIA
CHHHHHHHHHHHHCCCCCCHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
FAQRRKTSRNAFVQWAGSGSESANRLLAASIDPARRGETLSIDDFVRLLRRSGGSDEATS
HHHHHHCCCCCEEEECCCCCCHHCEEEEECCCHHHCCCEECHHHHHHHHHHCCCCCCCCC
TGRDARAPDISGHASAS
CCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972