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The map label for this gene is ispE [H]

Identifier: 121636940

GI number: 121636940

Start: 1160680

End: 1161600

Strand: Direct

Name: ispE [H]

Synonym: BCG_1068

Alternate gene names: 121636940

Gene position: 1160680-1161600 (Clockwise)

Preceding gene: 121636939

Following gene: 121636941

Centisome position: 26.53

GC content: 68.51

Gene sequence:

>921_bases
GTGCCCACCGGGTCGGTCACCGTTCGGGTGCCCGGAAAGGTCAACCTCTATCTGGCGGTCGGCGATCGCCGCGAGGACGG
CTATCACGAGCTGACCACGGTATTTCATGCCGTCTCGCTGGTCGACGAGGTAACCGTTCGTAACGCTGATGTGCTCTCGC
TCGAGTTGGTCGGCGAGGGGGCCGACCAGCTGCCGACCGACGAACGCAATCTCGCCTGGCAGGCGGCCGAGCTGATGGCC
GAACACGTGGGCCGGGCGCCGGACGTCTCGATCATGATCGACAAATCCATTCCGGTCGCCGGCGGCATGGCCGGTGGCAG
CGCGGACGCTGCGGCGGTCCTGGTTGCGATGAACTCGTTGTGGGAACTCAATGTGCCCCGCCGCGACCTGCGCATGCTCG
CCGCGCGGCTAGGCAGCGATGTGCCGTTTGCCCTGCATGGTGGTACCGCGCTGGGGACGGGTCGCGGCGAGGAGTTGGCC
ACCGTGTTATCCCGCAACACCTTCCACTGGGTCCTGGCGTTCGCCGACAGCGGGTTGCTCACCTCCGCGGTGTACAACGA
GCTCGACCGGCTCAGGGAGGTGGGGGATCCGCCCCGGCTTGGTGAGCCCGGGCCGGTTCTGGCTGCCTTAGCTGCGGGTG
ATCCGGATCAGCTGGCGCCGTTGCTGGGTAATGAAATGCAAGCGGCCGCGGTGAGCCTGGACCCGGCGCTGGCTCGTGCG
TTACGCGCCGGTGTGGAGGCCGGCGCGCTCGCAGGCATCGTGTCCGGTTCGGGTCCCACGTGTGCCTTCCTGTGCACCTC
GGCGAGCTCGGCGATCGATGTCGGCGCGCAGCTGTCGGGGGCGGGAGTTTGTCGCACCGTTCGAGTCGCCACCGGGCCGG
TACCCGGCGCCCGCGTGGTGTCTGCGCCGACCGAAGTGTGA

Upstream 100 bases:

>100_bases
GCGTCGGCGAGCTGACGGGGCGCCGCCGCGTGTGGTCGGCGCGTCACAGCGATAGTCTGCTGCGGTGTCCGCATCTGACG
GCAACACCGCTGAATTGTGG

Downstream 100 bases:

>100_bases
CCGAATTCTTGGGAGCATGCCTCGGGCGGCCAGGGGTATCCGCGCGTGCCGAGGCCGGTGGGTCGATCGGCTGGCGCACC
AGCATGCCAGCGGTAGGGCC

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MPTGSVTVRVPGKVNLYLAVGDRREDGYHELTTVFHAVSLVDEVTVRNADVLSLELVGEGADQLPTDERNLAWQAAELMA
EHVGRAPDVSIMIDKSIPVAGGMAGGSADAAAVLVAMNSLWELNVPRRDLRMLAARLGSDVPFALHGGTALGTGRGEELA
TVLSRNTFHWVLAFADSGLLTSAVYNELDRLREVGDPPRLGEPGPVLAALAAGDPDQLAPLLGNEMQAAAVSLDPALARA
LRAGVEAGALAGIVSGSGPTCAFLCTSASSAIDVGAQLSGAGVCRTVRVATGPVPGARVVSAPTEV

Sequences:

>Translated_306_residues
MPTGSVTVRVPGKVNLYLAVGDRREDGYHELTTVFHAVSLVDEVTVRNADVLSLELVGEGADQLPTDERNLAWQAAELMA
EHVGRAPDVSIMIDKSIPVAGGMAGGSADAAAVLVAMNSLWELNVPRRDLRMLAARLGSDVPFALHGGTALGTGRGEELA
TVLSRNTFHWVLAFADSGLLTSAVYNELDRLREVGDPPRLGEPGPVLAALAAGDPDQLAPLLGNEMQAAAVSLDPALARA
LRAGVEAGALAGIVSGSGPTCAFLCTSASSAIDVGAQLSGAGVCRTVRVATGPVPGARVVSAPTEV
>Mature_305_residues
PTGSVTVRVPGKVNLYLAVGDRREDGYHELTTVFHAVSLVDEVTVRNADVLSLELVGEGADQLPTDERNLAWQAAELMAE
HVGRAPDVSIMIDKSIPVAGGMAGGSADAAAVLVAMNSLWELNVPRRDLRMLAARLGSDVPFALHGGTALGTGRGEELAT
VLSRNTFHWVLAFADSGLLTSAVYNELDRLREVGDPPRLGEPGPVLAALAAGDPDQLAPLLGNEMQAAAVSLDPALARAL
RAGVEAGALAGIVSGSGPTCAFLCTSASSAIDVGAQLSGAGVCRTVRVATGPVPGARVVSAPTEV

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol [H]

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787459, Length=160, Percent_Identity=37.5, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1786184, Length=292, Percent_Identity=26.027397260274, Blast_Score=68, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721 [H]

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N [H]

EC number: =2.7.1.148 [H]

Molecular weight: Translated: 31382; Mature: 31251

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTGSVTVRVPGKVNLYLAVGDRREDGYHELTTVFHAVSLVDEVTVRNADVLSLELVGEG
CCCCCEEEEECCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
ADQLPTDERNLAWQAAELMAEHVGRAPDVSIMIDKSIPVAGGMAGGSADAAAVLVAMNSL
CCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHH
WELNVPRRDLRMLAARLGSDVPFALHGGTALGTGRGEELATVLSRNTFHWVLAFADSGLL
HCCCCCHHHHHHHHHHHCCCCCEEEECCCEECCCCCHHHHHHHHCCCEEEEEEEECCCHH
TSAVYNELDRLREVGDPPRLGEPGPVLAALAAGDPDQLAPLLGNEMQAAAVSLDPALARA
HHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHCCCHHHHHEECCHHHHHH
LRAGVEAGALAGIVSGSGPTCAFLCTSASSAIDVGAQLSGAGVCRTVRVATGPVPGARVV
HHHCCCCCCEEEEECCCCCEEEEEEECCCCHHHCCCCCCCCCCEEEEEEECCCCCCCEEE
SAPTEV
CCCCCC
>Mature Secondary Structure 
PTGSVTVRVPGKVNLYLAVGDRREDGYHELTTVFHAVSLVDEVTVRNADVLSLELVGEG
CCCCEEEEECCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
ADQLPTDERNLAWQAAELMAEHVGRAPDVSIMIDKSIPVAGGMAGGSADAAAVLVAMNSL
CCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHH
WELNVPRRDLRMLAARLGSDVPFALHGGTALGTGRGEELATVLSRNTFHWVLAFADSGLL
HCCCCCHHHHHHHHHHHCCCCCEEEECCCEECCCCCHHHHHHHHCCCEEEEEEEECCCHH
TSAVYNELDRLREVGDPPRLGEPGPVLAALAAGDPDQLAPLLGNEMQAAAVSLDPALARA
HHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHCCCHHHHHEECCHHHHHH
LRAGVEAGALAGIVSGSGPTCAFLCTSASSAIDVGAQLSGAGVCRTVRVATGPVPGARVV
HHHCCCCCCEEEEECCCCCEEEEEEECCCCHHHCCCCCCCCCCEEEEEEECCCCCCCEEE
SAPTEV
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA