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The map label for this gene is gcvA [H]

Identifier: 120613266

GI number: 120613266

Start: 5158483

End: 5159406

Strand: Direct

Name: gcvA [H]

Synonym: Aave_4635

Alternate gene names: 120613266

Gene position: 5158483-5159406 (Clockwise)

Preceding gene: 120613261

Following gene: 120613271

Centisome position: 96.37

GC content: 73.05

Gene sequence:

>924_bases
ATGCCCCTGCGCCGGCTCAATCCCCCGCTGCACCTGCTGCGAACGCTCTGCACCGTGGTCCGCTGCGGCAATGCATCGGC
GGCGGCGGAATCGCTGGACCTCACCCAGAGCGCCGTGAGCAAGCAGATCCAGGAGCTCGAGCGCTGGGTGGGCGTGCCGC
TGTTCGAGCGCAGCCGCCAGCGCCTGGTGCTCACTCCCGCGGGCGAGCGCTACGAATTGACGGTACGGGCCCTGCTGGCA
CGCCTGGAAGCCGCCACGCTCGAACTCGTGGCCAGCGGGTACGACGGCGGCGCGCTGCATATCGCCGCGCTGCCCACCTT
CTGCGCGCAGTGGCTGGTGCCCCGTCTGCCGGATTTCCGGCGGCGGCATCCGCGCATCACGGTGCACCTGGCGGAGCCGG
CCGTCCATGGTCCGCCGGAGCCGGGAACGGACTGCGCCATCCTGTTCGGCGACGGGCACTGGCCCGGCATGCATGCCCAC
TACATCGCCGGCAACGACATGGCCCTGATCGCGCCGCCCGGCGCAGGCGGCGCGGAGCCCCCCTCGCCGGTGCTGCGCAG
CCCCGCCGATGCCGCAGGGCATACGCTGTTGCGGCACGGGTCCGCGCCCGACATCTGGGAACGCTGGAGCGAGCGCCACG
GCGTGCCCGGGCTGAACTGCTTCGATGGTCCGGTGCTCGATCTGTTCGAGAGCATCGTCCGGGGCGTCGCCATGGGCCTG
GGCCTCGGGCTGGTGCCCCGCTGCCTCGTGCGCGACGAGATCGCGGCCGGCATCGTCTCGGAACCACTGCCGGCGGCCGG
CTTCAACAGCCGGCTGGGCTACTGGCTCTGCCATCCGGGCGGGCGCACGCGGTCGCCTGCGCTGGGCCATTTCCGCGACT
GGCTGCTGCAGCAGGCCGAGCCCCCCGCCGGTCCCGCGGCCTGA

Upstream 100 bases:

>100_bases
GAGATTACTGGAATAAATGCATGAATGACGCAAAGGGCATGCCGGTTAGCATTCCGATTCGGCATACCCTCATCGCTGTT
CGTCCCCGGAACCGTCCCCC

Downstream 100 bases:

>100_bases
ACGCCCCCTCCCCGGTGGCGGCGCAGAAAGGCGGCTGAACCGCCGTTTCAACCATCTTCTGCCCGAAGCCGTGCGCTTTC
GCACAGACGCGAACGTCTCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MPLRRLNPPLHLLRTLCTVVRCGNASAAAESLDLTQSAVSKQIQELERWVGVPLFERSRQRLVLTPAGERYELTVRALLA
RLEAATLELVASGYDGGALHIAALPTFCAQWLVPRLPDFRRRHPRITVHLAEPAVHGPPEPGTDCAILFGDGHWPGMHAH
YIAGNDMALIAPPGAGGAEPPSPVLRSPADAAGHTLLRHGSAPDIWERWSERHGVPGLNCFDGPVLDLFESIVRGVAMGL
GLGLVPRCLVRDEIAAGIVSEPLPAAGFNSRLGYWLCHPGGRTRSPALGHFRDWLLQQAEPPAGPAA

Sequences:

>Translated_307_residues
MPLRRLNPPLHLLRTLCTVVRCGNASAAAESLDLTQSAVSKQIQELERWVGVPLFERSRQRLVLTPAGERYELTVRALLA
RLEAATLELVASGYDGGALHIAALPTFCAQWLVPRLPDFRRRHPRITVHLAEPAVHGPPEPGTDCAILFGDGHWPGMHAH
YIAGNDMALIAPPGAGGAEPPSPVLRSPADAAGHTLLRHGSAPDIWERWSERHGVPGLNCFDGPVLDLFESIVRGVAMGL
GLGLVPRCLVRDEIAAGIVSEPLPAAGFNSRLGYWLCHPGGRTRSPALGHFRDWLLQQAEPPAGPAA
>Mature_306_residues
PLRRLNPPLHLLRTLCTVVRCGNASAAAESLDLTQSAVSKQIQELERWVGVPLFERSRQRLVLTPAGERYELTVRALLAR
LEAATLELVASGYDGGALHIAALPTFCAQWLVPRLPDFRRRHPRITVHLAEPAVHGPPEPGTDCAILFGDGHWPGMHAHY
IAGNDMALIAPPGAGGAEPPSPVLRSPADAAGHTLLRHGSAPDIWERWSERHGVPGLNCFDGPVLDLFESIVRGVAMGLG
LGLVPRCLVRDEIAAGIVSEPLPAAGFNSRLGYWLCHPGGRTRSPALGHFRDWLLQQAEPPAGPAA

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=293, Percent_Identity=31.740614334471, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1786448, Length=292, Percent_Identity=28.0821917808219, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788706, Length=303, Percent_Identity=26.0726072607261, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI145693193, Length=298, Percent_Identity=29.1946308724832, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1790262, Length=289, Percent_Identity=27.681660899654, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1786401, Length=163, Percent_Identity=31.9018404907975, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI157672245, Length=163, Percent_Identity=31.2883435582822, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788481, Length=306, Percent_Identity=27.4509803921569, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1788887, Length=119, Percent_Identity=34.453781512605, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33036; Mature: 32905

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLRRLNPPLHLLRTLCTVVRCGNASAAAESLDLTQSAVSKQIQELERWVGVPLFERSRQ
CCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCC
RLVLTPAGERYELTVRALLARLEAATLELVASGYDGGALHIAALPTFCAQWLVPRLPDFR
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHCCCHHH
RRHPRITVHLAEPAVHGPPEPGTDCAILFGDGHWPGMHAHYIAGNDMALIAPPGAGGAEP
HCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCEEEEECCCCCCCCC
PSPVLRSPADAAGHTLLRHGSAPDIWERWSERHGVPGLNCFDGPVLDLFESIVRGVAMGL
CCHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
GLGLVPRCLVRDEIAAGIVSEPLPAAGFNSRLGYWLCHPGGRTRSPALGHFRDWLLQQAE
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHCC
PPAGPAA
CCCCCCC
>Mature Secondary Structure 
PLRRLNPPLHLLRTLCTVVRCGNASAAAESLDLTQSAVSKQIQELERWVGVPLFERSRQ
CCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCC
RLVLTPAGERYELTVRALLARLEAATLELVASGYDGGALHIAALPTFCAQWLVPRLPDFR
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHCCCHHH
RRHPRITVHLAEPAVHGPPEPGTDCAILFGDGHWPGMHAHYIAGNDMALIAPPGAGGAEP
HCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCEEEEECCCCCCCCC
PSPVLRSPADAAGHTLLRHGSAPDIWERWSERHGVPGLNCFDGPVLDLFESIVRGVAMGL
CCHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
GLGLVPRCLVRDEIAAGIVSEPLPAAGFNSRLGYWLCHPGGRTRSPALGHFRDWLLQQAE
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHCC
PPAGPAA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]