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The map label for this gene is birA [H]

Identifier: 120613260

GI number: 120613260

Start: 5151502

End: 5152353

Strand: Direct

Name: birA [H]

Synonym: Aave_4629

Alternate gene names: 120613260

Gene position: 5151502-5152353 (Clockwise)

Preceding gene: 120613259

Following gene: 120613261

Centisome position: 96.24

GC content: 75.7

Gene sequence:

>852_bases
ATGACCGGCACGGCGCAGCCGCTGCGCTGGCCCGCCGAGGCGGTGTGGGAGGCCGTGGCCCCGCTGCTGCCGGGCTTCAC
CGTCGAAGTGCTGCCGAGCATAGACTCCACCAACACCGAACTCATGCGGCGCGCGCGCGCGGGCCGCTGCGAGCCCACGC
TGCTCGTGGCCGAGGTGCAGACCGCCGGGCGCGGGCGCCTGGGCCGCGCGTGGCAGAACGCCGCGGGCGACTCGCTCATG
TTCTCGCTGGGCCTGCCGCTGGCGCCCGCGGACTGGTCCGGGCTGTCGCTGGCCGTGGGGGTCTCCGTCGCGGAAAGCCT
GCAGCCGGTGCCGCGTGCCGGCCAGCCCCGCCTGGGCCTGAAATGGCCCAACGACCTCTGGCTGGACGACGGCCGCAAGC
TGGGAGGCATCCTGGTGGAAACCGCCAGCCTCGTGGGCGCTGCCGGCGCCTGGAGCGGCGGGCATCCGGACCGTACGGCG
CGCTACGTGGTGGCCGGGGTGGGCATCAACGTGCGGCCTCCGCCGGCCGAGGGCCTGTCCACGCCGCCCGGCAGTCTGCA
GGAGATCGATACCCGGCTCGACGCCCCCGCGGCACTGCTGCGCACCGTGCCGCCGCTGGTCGCGATGCTGCAGTCGTTCG
AAGCCCACGGCTTCGCGCCGGTGCAGCCGCGCTTCCTGCAGCGCGACGTGCTGCGCGACCGCGAAGTAGTCCTGAGCGAC
GGCGGCGCCGGCACCGCGCAGGGCGTGGCCCCGGACGGAGCGCTGCTGGTGCGCACGCCCCAGGGCCTGCAGGCCGTGAC
CAGCGCCGAGATCAGCGTGCGGCCGGCCGGGCAGGCGCTGCCGCGGAACTGA

Upstream 100 bases:

>100_bases
GCGCCAACTGCCGCGGCACGCTGCTGGCGCCCAAGCGCGGCTGGGCGCCGCCGGGGCCCACCGCGCCGCCGCCCAAGGCC
GGCCGCAAGGGCGCGCGCCG

Downstream 100 bases:

>100_bases
AGGAGCCTTCCACCATGCTGCGCGCCGCCGTCCTCGTTCTGCTGCTGGCCAATGCCGGCTATTACGCCTGGGCACAAGGC
CTGCTGCGCGACTGGGGCAT

Product: biotin--acetyl-CoA-carboxylase ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MTGTAQPLRWPAEAVWEAVAPLLPGFTVEVLPSIDSTNTELMRRARAGRCEPTLLVAEVQTAGRGRLGRAWQNAAGDSLM
FSLGLPLAPADWSGLSLAVGVSVAESLQPVPRAGQPRLGLKWPNDLWLDDGRKLGGILVETASLVGAAGAWSGGHPDRTA
RYVVAGVGINVRPPPAEGLSTPPGSLQEIDTRLDAPAALLRTVPPLVAMLQSFEAHGFAPVQPRFLQRDVLRDREVVLSD
GGAGTAQGVAPDGALLVRTPQGLQAVTSAEISVRPAGQALPRN

Sequences:

>Translated_283_residues
MTGTAQPLRWPAEAVWEAVAPLLPGFTVEVLPSIDSTNTELMRRARAGRCEPTLLVAEVQTAGRGRLGRAWQNAAGDSLM
FSLGLPLAPADWSGLSLAVGVSVAESLQPVPRAGQPRLGLKWPNDLWLDDGRKLGGILVETASLVGAAGAWSGGHPDRTA
RYVVAGVGINVRPPPAEGLSTPPGSLQEIDTRLDAPAALLRTVPPLVAMLQSFEAHGFAPVQPRFLQRDVLRDREVVLSD
GGAGTAQGVAPDGALLVRTPQGLQAVTSAEISVRPAGQALPRN
>Mature_282_residues
TGTAQPLRWPAEAVWEAVAPLLPGFTVEVLPSIDSTNTELMRRARAGRCEPTLLVAEVQTAGRGRLGRAWQNAAGDSLMF
SLGLPLAPADWSGLSLAVGVSVAESLQPVPRAGQPRLGLKWPNDLWLDDGRKLGGILVETASLVGAAGAWSGGHPDRTAR
YVVAGVGINVRPPPAEGLSTPPGSLQEIDTRLDAPAALLRTVPPLVAMLQSFEAHGFAPVQPRFLQRDVLRDREVVLSDG
GAGTAQGVAPDGALLVRTPQGLQAVTSAEISVRPAGQALPRN

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=261, Percent_Identity=36.7816091954023, Blast_Score=111, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR004409
- InterPro:   IPR003142
- InterPro:   IPR004143
- InterPro:   IPR013196
- InterPro:   IPR008988
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB; PF08279 HTH_11 [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 29601; Mature: 29470

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGTAQPLRWPAEAVWEAVAPLLPGFTVEVLPSIDSTNTELMRRARAGRCEPTLLVAEVQ
CCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCCCCCEEEEEEEC
TAGRGRLGRAWQNAAGDSLMFSLGLPLAPADWSGLSLAVGVSVAESLQPVPRAGQPRLGL
CCCCCCCCHHHHHCCCCEEEEECCCCCCCCCCCCCEEEEHHHHHHHCCCCCCCCCCCCCC
KWPNDLWLDDGRKLGGILVETASLVGAAGAWSGGHPDRTARYVVAGVGINVRPPPAEGLS
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCC
TPPGSLQEIDTRLDAPAALLRTVPPLVAMLQSFEAHGFAPVQPRFLQRDVLRDREVVLSD
CCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEEEC
GGAGTAQGVAPDGALLVRTPQGLQAVTSAEISVRPAGQALPRN
CCCCCCCCCCCCCEEEEECCCCHHHHHHCEEEECCCCCCCCCC
>Mature Secondary Structure 
TGTAQPLRWPAEAVWEAVAPLLPGFTVEVLPSIDSTNTELMRRARAGRCEPTLLVAEVQ
CCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCCCCCEEEEEEEC
TAGRGRLGRAWQNAAGDSLMFSLGLPLAPADWSGLSLAVGVSVAESLQPVPRAGQPRLGL
CCCCCCCCHHHHHCCCCEEEEECCCCCCCCCCCCCEEEEHHHHHHHCCCCCCCCCCCCCC
KWPNDLWLDDGRKLGGILVETASLVGAAGAWSGGHPDRTARYVVAGVGINVRPPPAEGLS
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCC
TPPGSLQEIDTRLDAPAALLRTVPPLVAMLQSFEAHGFAPVQPRFLQRDVLRDREVVLSD
CCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEEEC
GGAGTAQGVAPDGALLVRTPQGLQAVTSAEISVRPAGQALPRN
CCCCCCCCCCCCCEEEEECCCCHHHHHHCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3899863; 3536662; 8265357; 9278503; 1311302; 1328157; 1409631; 11353844 [H]