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The map label for this gene is ogt [H]

Identifier: 120611488

GI number: 120611488

Start: 3099952

End: 3100560

Strand: Direct

Name: ogt [H]

Synonym: Aave_2824

Alternate gene names: 120611488

Gene position: 3099952-3100560 (Clockwise)

Preceding gene: 120611487

Following gene: 120611489

Centisome position: 57.91

GC content: 74.71

Gene sequence:

>609_bases
ATGCCGCTCGCACCCTCCTCCCGCCCCGCCCTCGTCCAGATGCGCATCGCCAGCCCGCTCGGCGACATGCGCCTCGCCGC
CTCGGCCGCAGGCCTGGCCGGGATCTGGTTCGACGGACAGCGCCACCAGCCCACCGACCGCCTCGACGGCCCCGGAGCCT
GGCAGCACGACCCTTCGCACCCCGTCCTGGTCGCCACGGCATCGCAACTGCGCGAATACCTGGAAGGCCGGCGCAGCCGG
TTCGACGTTCCTCTCGATCTCACGGTCGGCACCGCCTTCCAGCGCGATGTCTGGCAGGCCCTGGTGGAGATACCGCACGG
CGCCGTCACCAGCTACGCGGCCCTGGCTCGCGCCCTGGGCCGTCCGCTCGCGGTGCGGGCCGTGGGAGCGGCGGTCGGGC
GCAATCCGGTCAGCGTGATCGTGCCGTGCCACCGCGTGCTCGGATCGGACGGCACCCTCACCGGATACGCTGGCGGCCTG
CCGCGCAAAACCGCCCTGCTGAGGCTGGAAGGGGCTCTTGCGGACCGCCTGCGCACCTTGCCCCTCTGGCCGCAGGGCGA
CGCCACGGCCCGCGGCACCGGCGCCCCGCGCACCGGGGCGGCGGCGTGA

Upstream 100 bases:

>100_bases
GCCGAATCCCGGCCGCCGCCGACCTAGCCGCACGCCACGCCGCCACCTGCCTCCCCCCCCCGGCGCCCCGGTCCTCCATT
TCCATCGACATACTTCCGCC

Downstream 100 bases:

>100_bases
ACGCGGCGGCCGGCGTGCTGCCTGCCCGCCACTGGATCGGTGAATTCCTGCTGCTTTCCGCCCTCTGGGGGGCGTCGTTC
CTCTTCATGCGCCTGGGCGC

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 202; Mature: 201

Protein sequence:

>202_residues
MPLAPSSRPALVQMRIASPLGDMRLAASAAGLAGIWFDGQRHQPTDRLDGPGAWQHDPSHPVLVATASQLREYLEGRRSR
FDVPLDLTVGTAFQRDVWQALVEIPHGAVTSYAALARALGRPLAVRAVGAAVGRNPVSVIVPCHRVLGSDGTLTGYAGGL
PRKTALLRLEGALADRLRTLPLWPQGDATARGTGAPRTGAAA

Sequences:

>Translated_202_residues
MPLAPSSRPALVQMRIASPLGDMRLAASAAGLAGIWFDGQRHQPTDRLDGPGAWQHDPSHPVLVATASQLREYLEGRRSR
FDVPLDLTVGTAFQRDVWQALVEIPHGAVTSYAALARALGRPLAVRAVGAAVGRNPVSVIVPCHRVLGSDGTLTGYAGGL
PRKTALLRLEGALADRLRTLPLWPQGDATARGTGAPRTGAAA
>Mature_201_residues
PLAPSSRPALVQMRIASPLGDMRLAASAAGLAGIWFDGQRHQPTDRLDGPGAWQHDPSHPVLVATASQLREYLEGRRSRF
DVPLDLTVGTAFQRDVWQALVEIPHGAVTSYAALARALGRPLAVRAVGAAVGRNPVSVIVPCHRVLGSDGTLTGYAGGLP
RKTALLRLEGALADRLRTLPLWPQGDATARGTGAPRTGAAA

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=162, Percent_Identity=34.5679012345679, Blast_Score=77, Evalue=9e-15,
Organism=Escherichia coli, GI1787596, Length=107, Percent_Identity=57.0093457943925, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1788542, Length=103, Percent_Identity=50.4854368932039, Blast_Score=102, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI115533070, Length=82, Percent_Identity=51.219512195122, Blast_Score=87, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI115533068, Length=82, Percent_Identity=51.219512195122, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6320001, Length=88, Percent_Identity=43.1818181818182, Blast_Score=92, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 21240; Mature: 21108

Theoretical pI: Translated: 11.35; Mature: 11.35

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLAPSSRPALVQMRIASPLGDMRLAASAAGLAGIWFDGQRHQPTDRLDGPGAWQHDPSH
CCCCCCCCCCEEEEEECCCCCHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCC
PVLVATASQLREYLEGRRSRFDVPLDLTVGTAFQRDVWQALVEIPHGAVTSYAALARALG
CEEEECHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHC
RPLAVRAVGAAVGRNPVSVIVPCHRVLGSDGTLTGYAGGLPRKTALLRLEGALADRLRTL
CCHHHHHHHHHHCCCCEEEEEEHHHHHCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHCC
PLWPQGDATARGTGAPRTGAAA
CCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PLAPSSRPALVQMRIASPLGDMRLAASAAGLAGIWFDGQRHQPTDRLDGPGAWQHDPSH
CCCCCCCCCEEEEEECCCCCHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCC
PVLVATASQLREYLEGRRSRFDVPLDLTVGTAFQRDVWQALVEIPHGAVTSYAALARALG
CEEEECHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHC
RPLAVRAVGAAVGRNPVSVIVPCHRVLGSDGTLTGYAGGLPRKTALLRLEGALADRLRTL
CCHHHHHHHHHHCCCCEEEEEEHHHHHCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHCC
PLWPQGDATARGTGAPRTGAAA
CCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA