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The map label for this gene is ada [H]

Identifier: 120611487

GI number: 120611487

Start: 3098274

End: 3099878

Strand: Direct

Name: ada [H]

Synonym: Aave_2823

Alternate gene names: 120611487

Gene position: 3098274-3099878 (Clockwise)

Preceding gene: 120611482

Following gene: 120611488

Centisome position: 57.88

GC content: 74.02

Gene sequence:

>1605_bases
ATGATGGCCTGCATGGACTCACCACTCGCCCGCCCCGCCCCGCCCATCGCCGATGCGCTGGACGATGGCCGCTATCTGGC
CCTCGCGGCGCGGGACGCGCGTTTCGACGGACGGTTCTTCACCGGCGTCACGTCCACGGGCATCTACTGCCGGCCGGTCT
GCGCGGTCCGCACGCCGCGCCGCGAGAACTGCCGCTTTTTCGCGCTGGCCGCCCAGGCGGAGAATGCCGGCTTCCGGCCC
TGCCTGCGCTGCCGACCCGAGCTTGCACCGCGCGTGCCTGCCTGGTCCGTGCAGGACGCGCGCAGCATCCTGGTGCGCGA
GGCATTGCGGTTCCTGGATGCGGAAGGCGCCTGGGACACGGCCGCTGCGAGCGCCGGCGGCGCGGCGCCCGTGCAGCGCC
TGGCGGCCCGCCTGGGCGTGAGCGACCGGCACCTGCGCCGCGTGTTCGAGGCGGCCCTGGGCATCTCGCCGCTGCAATAC
CTGCAGACGCGCCGGCTGCTGACGGCCAAGCAGTTGCTCACCGATACCGATCTGCCCGTCACCCAGATCGCGCTGGCCAG
CGGCTTCGGCAGTGTGCGGCGGTTCAATGCCGCCTTTGCGCAGCACTATCGCCTGCAGCCCACCGCATTGCGCCGCGCGG
GCGGCACGGCCACCGCCGGGACAGCCTCCGGCACGGTGCGCCTGGCCTACCGGCCACCGCTGGACATCGCGGCTCTGCTG
GGTTTCTTCGGGCAGCGCCGCATCCACGGCATGGAAACCGTGGATGTCCCCGGCCTGGAGCTGCGCCGGACGGCGAGGCT
GCAGGACGCGGAAGGCCGCGAATGCACCGGCTGGCTGGCAGCGCGCTTCGATGGCGGCGCGGCGGCCGCACGCGGAGGTC
CGCCGAAACCGCATGTGGTGCTGCGCGTGTCCAGCAGCCTGCTCCCGGCACTGCCGGGCGTGATTGCCCGGGTGCGCGGC
CTGCTGGACCTGGATGCGGATCCCGAGGCGATCAACGCGGTGCTGCACGGCGATTTTCCGCGCGGTGACGGTTTGCGCGT
ACCGGGTGCGTGGGACGGTTTCGAACTTGCCGTGCGGGCAGTGCTGGGCCAGCAGGTCACGGTCGCCGCCGCGCGGACCC
TGGCGCAGCGCGTGGTGGAGCGCTGGGGCGATCCCGTCGCCACTCCCTGGCCGGATCTTTGCCGGCTGTTCCCCACGCCC
GCCGTGCTGGCGGCCTGCGACGGCGACGCGCTGGGCCAGCTCGGCATCGTGCGCCAGCGCCAGGCCGCCATCGTGGCGCT
GTCCCGCGCGGTGGCGGAAGGCCGCCTCCTGCTGCACGCGGCGGCGGACGTGGCGGGCACCATCGCGGCCCTGCGCGCTC
TGCCCGGCATCGGCGACTGGACGGCACAGTACATCGCCATGCGCGCGCTGCGGTGGCCGGATGCCTTTCCCTCGGGCGAC
GTCGCGCTGCACAAGGCCCTGGCCGTGCAGTCCGCTCCCCGGCCGGCCAGGGCCGCCGAAGAAGCCTCCCAGGCATGGCG
GCCATGGCGCAGCTATGCCGTCGTGCGTGCCTGGGCCGGCACCGGCACGCCGGAGCCGGCCGAATCCCGGCCGCCGCCGA
CCTAG

Upstream 100 bases:

>100_bases
CGCTCGTAGGCGAAGGCCGCTTCTTGGCGACTGCCTGCCCGGCGCCCGGCGGTGACAGGCACACGTCCGGAAACGGCCAG
CGCCGCGCCGTCCGGCGCCT

Downstream 100 bases:

>100_bases
CCGCACGCCACGCCGCCACCTGCCTCCCCCCCCCGGCGCCCCGGTCCTCCATTTCCATCGACATACTTCCGCCATGCCGC
TCGCACCCTCCTCCCGCCCC

Product: DNA-O6-methylguanine--protein-cysteine S-methyltransferase / DNA-3-methyladenine glycosylase II / transcriptional regulator Ada

Products: NA

Alternate protein names: Regulatory protein of adaptative response; Methylated-DNA--protein-cysteine methyltransferase; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 534; Mature: 534

Protein sequence:

>534_residues
MMACMDSPLARPAPPIADALDDGRYLALAARDARFDGRFFTGVTSTGIYCRPVCAVRTPRRENCRFFALAAQAENAGFRP
CLRCRPELAPRVPAWSVQDARSILVREALRFLDAEGAWDTAAASAGGAAPVQRLAARLGVSDRHLRRVFEAALGISPLQY
LQTRRLLTAKQLLTDTDLPVTQIALASGFGSVRRFNAAFAQHYRLQPTALRRAGGTATAGTASGTVRLAYRPPLDIAALL
GFFGQRRIHGMETVDVPGLELRRTARLQDAEGRECTGWLAARFDGGAAAARGGPPKPHVVLRVSSSLLPALPGVIARVRG
LLDLDADPEAINAVLHGDFPRGDGLRVPGAWDGFELAVRAVLGQQVTVAAARTLAQRVVERWGDPVATPWPDLCRLFPTP
AVLAACDGDALGQLGIVRQRQAAIVALSRAVAEGRLLLHAAADVAGTIAALRALPGIGDWTAQYIAMRALRWPDAFPSGD
VALHKALAVQSAPRPARAAEEASQAWRPWRSYAVVRAWAGTGTPEPAESRPPPT

Sequences:

>Translated_534_residues
MMACMDSPLARPAPPIADALDDGRYLALAARDARFDGRFFTGVTSTGIYCRPVCAVRTPRRENCRFFALAAQAENAGFRP
CLRCRPELAPRVPAWSVQDARSILVREALRFLDAEGAWDTAAASAGGAAPVQRLAARLGVSDRHLRRVFEAALGISPLQY
LQTRRLLTAKQLLTDTDLPVTQIALASGFGSVRRFNAAFAQHYRLQPTALRRAGGTATAGTASGTVRLAYRPPLDIAALL
GFFGQRRIHGMETVDVPGLELRRTARLQDAEGRECTGWLAARFDGGAAAARGGPPKPHVVLRVSSSLLPALPGVIARVRG
LLDLDADPEAINAVLHGDFPRGDGLRVPGAWDGFELAVRAVLGQQVTVAAARTLAQRVVERWGDPVATPWPDLCRLFPTP
AVLAACDGDALGQLGIVRQRQAAIVALSRAVAEGRLLLHAAADVAGTIAALRALPGIGDWTAQYIAMRALRWPDAFPSGD
VALHKALAVQSAPRPARAAEEASQAWRPWRSYAVVRAWAGTGTPEPAESRPPPT
>Mature_534_residues
MMACMDSPLARPAPPIADALDDGRYLALAARDARFDGRFFTGVTSTGIYCRPVCAVRTPRRENCRFFALAAQAENAGFRP
CLRCRPELAPRVPAWSVQDARSILVREALRFLDAEGAWDTAAASAGGAAPVQRLAARLGVSDRHLRRVFEAALGISPLQY
LQTRRLLTAKQLLTDTDLPVTQIALASGFGSVRRFNAAFAQHYRLQPTALRRAGGTATAGTASGTVRLAYRPPLDIAALL
GFFGQRRIHGMETVDVPGLELRRTARLQDAEGRECTGWLAARFDGGAAAARGGPPKPHVVLRVSSSLLPALPGVIARVRG
LLDLDADPEAINAVLHGDFPRGDGLRVPGAWDGFELAVRAVLGQQVTVAAARTLAQRVVERWGDPVATPWPDLCRLFPTP
AVLAACDGDALGQLGIVRQRQAAIVALSRAVAEGRLLLHAAADVAGTIAALRALPGIGDWTAQYIAMRALRWPDAFPSGD
VALHKALAVQSAPRPARAAEEASQAWRPWRSYAVVRAWAGTGTPEPAESRPPPT

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus [H]

COG id: COG0122

COG function: function code L; 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788383, Length=295, Percent_Identity=32.5423728813559, Blast_Score=132, Evalue=7e-32,
Organism=Escherichia coli, GI1788542, Length=169, Percent_Identity=35.5029585798817, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR010316
- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR003583
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR023170
- InterPro:   IPR012294 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF06029 AlkA_N; PF00730 HhH-GPD [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 57052; Mature: 57052

Theoretical pI: Translated: 10.57; Mature: 10.57

Prosite motif: PS01124 HTH_ARAC_FAMILY_2 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMACMDSPLARPAPPIADALDDGRYLALAARDARFDGRFFTGVTSTGIYCRPVCAVRTPR
CCCCCCCCCCCCCCCHHHHCCCCCEEEEEECCCCCCCEEEECCCCCCCEEEEHHHCCCCC
RENCRFFALAAQAENAGFRPCLRCRPELAPRVPAWSVQDARSILVREALRFLDAEGAWDT
CCCCEEEEEEEECCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCH
AAASAGGAAPVQRLAARLGVSDRHLRRVFEAALGISPLQYLQTRRLLTAKQLLTDTDLPV
HHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCH
TQIALASGFGSVRRFNAAFAQHYRLQPTALRRAGGTATAGTASGTVRLAYRPPLDIAALL
HHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHH
GFFGQRRIHGMETVDVPGLELRRTARLQDAEGRECTGWLAARFDGGAAAARGGPPKPHVV
HHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEE
LRVSSSLLPALPGVIARVRGLLDLDADPEAINAVLHGDFPRGDGLRVPGAWDGFELAVRA
EEECCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHH
VLGQQVTVAAARTLAQRVVERWGDPVATPWPDLCRLFPTPAVLAACDGDALGQLGIVRQR
HHCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCEEEECCCCHHHHHHHHHHH
QAAIVALSRAVAEGRLLLHAAADVAGTIAALRALPGIGDWTAQYIAMRALRWPDAFPSGD
HHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCC
VALHKALAVQSAPRPARAAEEASQAWRPWRSYAVVRAWAGTGTPEPAESRPPPT
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MMACMDSPLARPAPPIADALDDGRYLALAARDARFDGRFFTGVTSTGIYCRPVCAVRTPR
CCCCCCCCCCCCCCCHHHHCCCCCEEEEEECCCCCCCEEEECCCCCCCEEEEHHHCCCCC
RENCRFFALAAQAENAGFRPCLRCRPELAPRVPAWSVQDARSILVREALRFLDAEGAWDT
CCCCEEEEEEEECCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCH
AAASAGGAAPVQRLAARLGVSDRHLRRVFEAALGISPLQYLQTRRLLTAKQLLTDTDLPV
HHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCH
TQIALASGFGSVRRFNAAFAQHYRLQPTALRRAGGTATAGTASGTVRLAYRPPLDIAALL
HHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHH
GFFGQRRIHGMETVDVPGLELRRTARLQDAEGRECTGWLAARFDGGAAAARGGPPKPHVV
HHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEE
LRVSSSLLPALPGVIARVRGLLDLDADPEAINAVLHGDFPRGDGLRVPGAWDGFELAVRA
EEECCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHH
VLGQQVTVAAARTLAQRVVERWGDPVATPWPDLCRLFPTPAVLAACDGDALGQLGIVRQR
HHCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCEEEECCCCHHHHHHHHHHH
QAAIVALSRAVAEGRLLLHAAADVAGTIAALRALPGIGDWTAQYIAMRALRWPDAFPSGD
HHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCC
VALHKALAVQSAPRPARAAEEASQAWRPWRSYAVVRAWAGTGTPEPAESRPPPT
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]