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The map label for this gene is yoaE [H]

Identifier: 120609461

GI number: 120609461

Start: 822509

End: 824593

Strand: Reverse

Name: yoaE [H]

Synonym: Aave_0767

Alternate gene names: 120609461

Gene position: 824593-822509 (Counterclockwise)

Preceding gene: 120609463

Following gene: 120609451

Centisome position: 15.4

GC content: 71.8

Gene sequence:

>2085_bases
ATGCCCACGATGGAAACCATTTTGCACATTCCCGAGCCGTCCGCCGCCCCCCTGCAGGTGCGCGGCGCCTGCCCCCACGA
CTGCCCCGACACCTGCGCGCTGGTCACCACCGTGGAGAACGGCGTGGCCGTGCGGGTGCAGGGCAATCCGGCGCACGGGC
ACACCGACGGCGTGCTCTGCGCCAAGGTGTCGCGCTACGCCGAGCGCACGAACCATCCGGACCGCATCCTCACGCCGCTG
CGGCGCACGGGCCCCAAGGGCAGCGGGCAGTTCGAGCCGGTGGGCTGGGACGAGGCGCTGGCGGACATCGCGGCGCGCCT
GTCCGCGATCGCCGCGCGCGATCCGCAGGCCATCCTGCCCTACAGCTATGCCGGCACCATGGGGCTGGTGCAGGGCGAGA
GCATGGACCGGCGGTTCTTCCACCGCCTGGGCGCCTCGCTGCTGGGGCGCACCATCTGCGCGGCCGCGGGCGGCGAAGGG
CTGAACCAGACGCTGGGCGGCAAGGTGGGCATGAAGGTGGAGCATTTCGCCGGGTCGCAGCTGATCCTCATCTGGGGCAG
CAATTCGATCGGCAGCAACCTGCACTTCTGGCGCTATGCGCAGCAGGCCAAGCGCGCGGGCGCCCGGCTGGTGTGCATCG
ACCCGCGCAGGACCGAGACGGCCGACAAGTGCCACGAGCACCTGCAACTGCTGCCCGGCACCGATGCGGCCCTGGCACTG
GCGATGATGCACGAGTTGATCGTGCACGACTGGCTGGACCACGACTACATCGCGCGCCACACGGTGGGCTGGGAAGGCCT
GCGCGAGCGGGCGCTCGCCTGGTCCCCTGAGCGCGCCGCGGCGGTCTGCGGCCTGCCGGTGGAGCAGATCCGCCAGCTGG
CGCGCGACTACGGCACCACGAAGCCCGCGGCCATCCGCCTCAACTACGGCATGCAGCGTGCGCGCGGCGGCGGCAATGCC
ACGCGCGCCGTGGCCTGCCTGCCCGCGCTCGTGGGCGCCTGGCGGCACCGGGCGGGCGGGCTGCTGCTCTCCAGCTCGGG
GCAGTTCCCCGTGCAGCGGCCCGTGCTGCACCGCCCCGACCTGATCCCCGCGGGCGTGCGGCCGCGCACCATCAACATGT
CCACCATCGGCGACGACCTGCTGCGCGCCGCCTCGCCCGGGTTCGGACCGCGCGTCGAGGCCGTGGTCGTCTACAACAGC
AATCCGGTGGCCGTGGCGCCGGAATCGGCCAAGGTGGTGCGCGGCTTCGCGCGGGACGACCTGTTCACCGTGGTGCTGGA
GCATTTCCAGACCGACACGGCCGATTACGCGGACTACGTGCTGCCCGCCACCACGCAGCTGGAGCACTGGGACGTGCACG
TGGCCTACGGCCACACCGACGTGCTGCTGAACCGGCCGGCCGTGGCGCCGCTCGGCCAGTCCAGGCCCAACACCCAGGTG
TTCCGCGAACTGGCCGCGCGCATGGGCTTCACGGAGCCCTGCTTCGCCGACAGCGACGAGGACCTCTGCCGGCAGGCCTA
TGGCCACCATGTGGACTTCCAGCAATTGCTGGACACGGGCTTCGCCACGCTGGCGATTCCCGATGCGCCATTCGCGGAAG
GCGGCTTCCCGACGCCCTCGGGCCGCTGCGAGTTCCACAGCGAGCGGCTGGCCGCGCAGGGGCTGGACCCGCTGCCGGAC
TACCTGCCCAACCACGAGCCCGCGCAGCCTGGCGGCCGCTATCCGCTGGCGATGATCTCGCCGCCGGCGCGCAACTTCCT
GAACTCCACCTTCGTGAACGTCACGAGCCTGCGCAACATCGAGACCGAGCCGCTGCTGGAGATGCACCCCGAGGACGCCG
CCGCGCGCGGCATCGCCGACGGCAGCACCGTGCGCGTGTTCAACGACCGCGGCAGCTATGCCTGCCGCGTGGCGCTCAAC
GGCCGCGCGCGCGCGGGCGTGGTGCACGGCCTGGGCATCTGGTGGCGCAAGTTCGGCCTGCAGGGCACGAACGTGAACGA
GGTGACGGGGCAGGCGCTGACGGACCTGGGGGCGGCGCCGACGTTCTATGACTGCGCGGTGGAAGTACAGGCCCAGGGGG
ACTGA

Upstream 100 bases:

>100_bases
TTGCGGCAGATCCGGCCCCGATGTTGTTGAAACCCTGGAAAGACCGCACCCCGCCTGTCCAGTCCGACTGCGGGCGCAGT
GTATCGCTCCGTCCCGCCCG

Downstream 100 bases:

>100_bases
CGACAGGCCCTGCTCGTCCACCGGAACGCTCAAGGGCTCAAGGAAGCACCTCGACGGCCTGCAGCCAGGCGTGGAAATGA
GGGCAGGCCGCCCTCATGGC

Product: molybdopterin oxidoreductase

Products: Reduced form of N-Oxide compounds [C]

Alternate protein names: NA

Number of amino acids: Translated: 694; Mature: 693

Protein sequence:

>694_residues
MPTMETILHIPEPSAAPLQVRGACPHDCPDTCALVTTVENGVAVRVQGNPAHGHTDGVLCAKVSRYAERTNHPDRILTPL
RRTGPKGSGQFEPVGWDEALADIAARLSAIAARDPQAILPYSYAGTMGLVQGESMDRRFFHRLGASLLGRTICAAAGGEG
LNQTLGGKVGMKVEHFAGSQLILIWGSNSIGSNLHFWRYAQQAKRAGARLVCIDPRRTETADKCHEHLQLLPGTDAALAL
AMMHELIVHDWLDHDYIARHTVGWEGLRERALAWSPERAAAVCGLPVEQIRQLARDYGTTKPAAIRLNYGMQRARGGGNA
TRAVACLPALVGAWRHRAGGLLLSSSGQFPVQRPVLHRPDLIPAGVRPRTINMSTIGDDLLRAASPGFGPRVEAVVVYNS
NPVAVAPESAKVVRGFARDDLFTVVLEHFQTDTADYADYVLPATTQLEHWDVHVAYGHTDVLLNRPAVAPLGQSRPNTQV
FRELAARMGFTEPCFADSDEDLCRQAYGHHVDFQQLLDTGFATLAIPDAPFAEGGFPTPSGRCEFHSERLAAQGLDPLPD
YLPNHEPAQPGGRYPLAMISPPARNFLNSTFVNVTSLRNIETEPLLEMHPEDAAARGIADGSTVRVFNDRGSYACRVALN
GRARAGVVHGLGIWWRKFGLQGTNVNEVTGQALTDLGAAPTFYDCAVEVQAQGD

Sequences:

>Translated_694_residues
MPTMETILHIPEPSAAPLQVRGACPHDCPDTCALVTTVENGVAVRVQGNPAHGHTDGVLCAKVSRYAERTNHPDRILTPL
RRTGPKGSGQFEPVGWDEALADIAARLSAIAARDPQAILPYSYAGTMGLVQGESMDRRFFHRLGASLLGRTICAAAGGEG
LNQTLGGKVGMKVEHFAGSQLILIWGSNSIGSNLHFWRYAQQAKRAGARLVCIDPRRTETADKCHEHLQLLPGTDAALAL
AMMHELIVHDWLDHDYIARHTVGWEGLRERALAWSPERAAAVCGLPVEQIRQLARDYGTTKPAAIRLNYGMQRARGGGNA
TRAVACLPALVGAWRHRAGGLLLSSSGQFPVQRPVLHRPDLIPAGVRPRTINMSTIGDDLLRAASPGFGPRVEAVVVYNS
NPVAVAPESAKVVRGFARDDLFTVVLEHFQTDTADYADYVLPATTQLEHWDVHVAYGHTDVLLNRPAVAPLGQSRPNTQV
FRELAARMGFTEPCFADSDEDLCRQAYGHHVDFQQLLDTGFATLAIPDAPFAEGGFPTPSGRCEFHSERLAAQGLDPLPD
YLPNHEPAQPGGRYPLAMISPPARNFLNSTFVNVTSLRNIETEPLLEMHPEDAAARGIADGSTVRVFNDRGSYACRVALN
GRARAGVVHGLGIWWRKFGLQGTNVNEVTGQALTDLGAAPTFYDCAVEVQAQGD
>Mature_693_residues
PTMETILHIPEPSAAPLQVRGACPHDCPDTCALVTTVENGVAVRVQGNPAHGHTDGVLCAKVSRYAERTNHPDRILTPLR
RTGPKGSGQFEPVGWDEALADIAARLSAIAARDPQAILPYSYAGTMGLVQGESMDRRFFHRLGASLLGRTICAAAGGEGL
NQTLGGKVGMKVEHFAGSQLILIWGSNSIGSNLHFWRYAQQAKRAGARLVCIDPRRTETADKCHEHLQLLPGTDAALALA
MMHELIVHDWLDHDYIARHTVGWEGLRERALAWSPERAAAVCGLPVEQIRQLARDYGTTKPAAIRLNYGMQRARGGGNAT
RAVACLPALVGAWRHRAGGLLLSSSGQFPVQRPVLHRPDLIPAGVRPRTINMSTIGDDLLRAASPGFGPRVEAVVVYNSN
PVAVAPESAKVVRGFARDDLFTVVLEHFQTDTADYADYVLPATTQLEHWDVHVAYGHTDVLLNRPAVAPLGQSRPNTQVF
RELAARMGFTEPCFADSDEDLCRQAYGHHVDFQQLLDTGFATLAIPDAPFAEGGFPTPSGRCEFHSERLAAQGLDPLPDY
LPNHEPAQPGGRYPLAMISPPARNFLNSTFVNVTSLRNIETEPLLEMHPEDAAARGIADGSTVRVFNDRGSYACRVALNG
RARAGVVHGLGIWWRKFGLQGTNVNEVTGQALTDLGAAPTFYDCAVEVQAQGD

Specific function: Terminal Reductase During Anaerobic Growth On Various Sulfoxide And N-Oxide Compounds. Allows E.Coli To Grow Anaerobically On Me(2)So As Respiratory Oxidant. [C]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm Face Of The Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081797, Length=731, Percent_Identity=26.812585499316, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI1787870, Length=727, Percent_Identity=26.1348005502063, Blast_Score=186, Evalue=5e-48,
Organism=Escherichia coli, GI171474008, Length=718, Percent_Identity=26.3231197771588, Blast_Score=186, Evalue=5e-48,
Organism=Escherichia coli, GI3868721, Length=725, Percent_Identity=26.7586206896552, Blast_Score=179, Evalue=5e-46,
Organism=Escherichia coli, GI145693196, Length=661, Percent_Identity=24.6596066565809, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI87081994, Length=673, Percent_Identity=22.5854383358098, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 75116; Mature: 74985

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS00490 MOLYBDOPTERIN_PROK_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTMETILHIPEPSAAPLQVRGACPHDCPDTCALVTTVENGVAVRVQGNPAHGHTDGVLC
CCCHHHEEECCCCCCCCEEECCCCCCCCCCCEEEEEEECCCEEEEEECCCCCCCCCCHHH
AKVSRYAERTNHPDRILTPLRRTGPKGSGQFEPVGWDEALADIAARLSAIAARDPQAILP
HHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECC
YSYAGTMGLVQGESMDRRFFHRLGASLLGRTICAAAGGEGLNQTLGGKVGMKVEHFAGSQ
CCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCEEEECCCCE
LILIWGSNSIGSNLHFWRYAQQAKRAGARLVCIDPRRTETADKCHEHLQLLPGTDAALAL
EEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHH
AMMHELIVHDWLDHDYIARHTVGWEGLRERALAWSPERAAAVCGLPVEQIRQLARDYGTT
HHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHCCCCCHHHEECCCCHHHHHHHHHHHCCC
KPAAIRLNYGMQRARGGGNATRAVACLPALVGAWRHRAGGLLLSSSGQFPVQRPVLHRPD
CCEEEEECCCHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCC
LIPAGVRPRTINMSTIGDDLLRAASPGFGPRVEAVVVYNSNPVAVAPESAKVVRGFARDD
CCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEECCCHHHHHHHHHHHH
LFTVVLEHFQTDTADYADYVLPATTQLEHWDVHVAYGHTDVLLNRPAVAPLGQSRPNTQV
HHHHHHHHHCCCCCHHHHHCCCCCCCEEEEEEEEEECCEEEEECCCCCCCCCCCCCCHHH
FRELAARMGFTEPCFADSDEDLCRQAYGHHVDFQQLLDTGFATLAIPDAPFAEGGFPTPS
HHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHCCCEEEEECCCCCCCCCCCCCC
GRCEFHSERLAAQGLDPLPDYLPNHEPAQPGGRYPLAMISPPARNFLNSTFVNVTSLRNI
CCCCHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCEEEECCHHHHHHHHHHEEHHHHCCC
ETEPLLEMHPEDAAARGIADGSTVRVFNDRGSYACRVALNGRARAGVVHGLGIWWRKFGL
CCCCHHHCCCCHHHHCCCCCCCEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHCC
QGTNVNEVTGQALTDLGAAPTFYDCAVEVQAQGD
CCCCCHHHHHHHHHHCCCCCCEEEEEEEEEECCC
>Mature Secondary Structure 
PTMETILHIPEPSAAPLQVRGACPHDCPDTCALVTTVENGVAVRVQGNPAHGHTDGVLC
CCHHHEEECCCCCCCCEEECCCCCCCCCCCEEEEEEECCCEEEEEECCCCCCCCCCHHH
AKVSRYAERTNHPDRILTPLRRTGPKGSGQFEPVGWDEALADIAARLSAIAARDPQAILP
HHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECC
YSYAGTMGLVQGESMDRRFFHRLGASLLGRTICAAAGGEGLNQTLGGKVGMKVEHFAGSQ
CCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCEEEECCCCE
LILIWGSNSIGSNLHFWRYAQQAKRAGARLVCIDPRRTETADKCHEHLQLLPGTDAALAL
EEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHH
AMMHELIVHDWLDHDYIARHTVGWEGLRERALAWSPERAAAVCGLPVEQIRQLARDYGTT
HHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHCCCCCHHHEECCCCHHHHHHHHHHHCCC
KPAAIRLNYGMQRARGGGNATRAVACLPALVGAWRHRAGGLLLSSSGQFPVQRPVLHRPD
CCEEEEECCCHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCC
LIPAGVRPRTINMSTIGDDLLRAASPGFGPRVEAVVVYNSNPVAVAPESAKVVRGFARDD
CCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEECCCHHHHHHHHHHHH
LFTVVLEHFQTDTADYADYVLPATTQLEHWDVHVAYGHTDVLLNRPAVAPLGQSRPNTQV
HHHHHHHHHCCCCCHHHHHCCCCCCCEEEEEEEEEECCEEEEECCCCCCCCCCCCCCHHH
FRELAARMGFTEPCFADSDEDLCRQAYGHHVDFQQLLDTGFATLAIPDAPFAEGGFPTPS
HHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHCCCEEEEECCCCCCCCCCCCCC
GRCEFHSERLAAQGLDPLPDYLPNHEPAQPGGRYPLAMISPPARNFLNSTFVNVTSLRNI
CCCCHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCEEEECCHHHHHHHHHHEEHHHHCCC
ETEPLLEMHPEDAAARGIADGSTVRVFNDRGSYACRVALNGRARAGVVHGLGIWWRKFGL
CCCCHHHCCCCHHHHCCCCCCCEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHCC
QGTNVNEVTGQALTDLGAAPTFYDCAVEVQAQGD
CCCCCHHHHHHHHHHCCCCCCEEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: Fe; Mo [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Oxide; Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

Specific reaction: Reduces Various N-Oxide And Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]