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The map label for this gene is 120599076

Identifier: 120599076

GI number: 120599076

Start: 2619350

End: 2620189

Strand: Direct

Name: 120599076

Synonym: Sputw3181_2272

Alternate gene names: NA

Gene position: 2619350-2620189 (Clockwise)

Preceding gene: 120599074

Following gene: 120599077

Centisome position: 55.63

GC content: 43.93

Gene sequence:

>840_bases
ATGAAATTAGAAACTATTGATTACCGTGCTGAAAATAGTGCACAGCAATTTGTTGAATCACTTAGAGAAACTGGCTTTGG
TGTTTTATCTAATCATCCTATTAATAAGCGCTTAGTTGAAGATATCTACACCGAGTGGCAAGCCTTTTTTAATTCAGAAG
CCAAAAACAACTATATGTTTAAAAAGGAAACCCACGACGGTTTCTTCCCTGCATCGATTTCTGAAACCGCCAAAGGCAAC
ACAGTCAAAGACATTAAAGAGTACTACCACGTGTATCCTTGGGGCCGTATACCCGACTCACTACGTGCCAATATTCTCGC
CTATTATGATAAAGCTAACGCGCTGGCCGCTGAGCTATTAGAGTGGATCGAAACCTACTCACCAGAAGACGTAAAAGCCA
AGTTTTCAATCCCATTGCCAGAAATGATCGCCAATAGCCAAAAAACCTTACTGCGTATTCTACATTACCCACCGATGACG
GGCAGTGAAGAAATGGGCGCTATTCGCGCCGCGGCCCATGAAGATATCAACCTTATCACAGTGTTACCTGCAGCCAATGA
GCCTGGATTGCAAGTAAAAGCCAAAGATGGCACTTGGTTAGATGTTCCAAGCGATTTTGGCAACATTATTATCAATATCG
GCGACATGTTACAGGAAGCCTCTGGCGGCTATTTCCCATCGACGTCACACCGCGTCATCAATCCTGAAGGTACGGATAAA
ACCAAGTCACGGATCTCTTTGCCGTTATTTTTACATCCAAACCCTGCAGTAGTGCTATCGGATCGTTATACCGCCGACAG
TTATCTGATGGAAAGATTGCGCGAACTAGGGGTGATCTAA

Upstream 100 bases:

>100_bases
TGATCAAGATCACAGTCGCTGAGTATTTGCACCCACCTCACCGCACGGCTACACTTGCAGCGGGTCAAATTACAACTAAA
TATAACAAGTAGAAACCAAT

Downstream 100 bases:

>100_bases
GCCCTAATTCAGCCTATTAATCCAAGTGACAATCGCAAGAGACAGTCACGGATTAAGCAATTAAAGCGCCTTTGGGCGCT
TTTTTTATGACATTCACCAC

Product: 2OG-Fe(II) oxygenase

Products: isopenicillin N; H2O

Alternate protein names: Oxidoreductase; 2OG-Fe(II) Oxygenase Family Oxidoreductase; Oxidoreductase 2OG-Fe(II) Oxygenase Family; Isopenicillin N Synthase; Iron/Ascorbate Family Oxidoreductase; 2OG-Fe(II) Oxygenase Superfamily Protein; Oxidoreductase 2OG-Fe(II) Oxygenase Family Protein; Flavonol Synthase/Dioxygenase; 2og-Fe(Ii) Oxygenase; Oxidoreductase Iron/Ascorbate Family Protein; Iron/Ascorbate Oxidoreductase; Iron/Ascorbate Oxidoreductase Family Protein; 1-Aminocyclopropane-1-Carboxylate Oxidase; Oxygenase; Isopenicillin N Synthase And Related Dioxygenases; Oxidoreductase 2og-Fe(Ii) Oxygenase Family; Iron/Ascorbate-Dependent Oxidoreductase Family Protein; Iron/Ascorbate-Dependent Oxidoreductase; Oxidoreductase Iron/Ascorbate Family; 2OG-Fe(II) Oxygenase Family Protein; Fe-Containing Oxidoreductase; Isopenicillin-N Synthase; 2-Oxobutyrate Oxidase; Flavonol Synthase/Flavanone 3-Hydroxylase; 2-Oxoglutarate And Fe(Ii)-Dependent Oxygenase; Oxygenase/Oxidoreductase

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MKLETIDYRAENSAQQFVESLRETGFGVLSNHPINKRLVEDIYTEWQAFFNSEAKNNYMFKKETHDGFFPASISETAKGN
TVKDIKEYYHVYPWGRIPDSLRANILAYYDKANALAAELLEWIETYSPEDVKAKFSIPLPEMIANSQKTLLRILHYPPMT
GSEEMGAIRAAAHEDINLITVLPAANEPGLQVKAKDGTWLDVPSDFGNIIINIGDMLQEASGGYFPSTSHRVINPEGTDK
TKSRISLPLFLHPNPAVVLSDRYTADSYLMERLRELGVI

Sequences:

>Translated_279_residues
MKLETIDYRAENSAQQFVESLRETGFGVLSNHPINKRLVEDIYTEWQAFFNSEAKNNYMFKKETHDGFFPASISETAKGN
TVKDIKEYYHVYPWGRIPDSLRANILAYYDKANALAAELLEWIETYSPEDVKAKFSIPLPEMIANSQKTLLRILHYPPMT
GSEEMGAIRAAAHEDINLITVLPAANEPGLQVKAKDGTWLDVPSDFGNIIINIGDMLQEASGGYFPSTSHRVINPEGTDK
TKSRISLPLFLHPNPAVVLSDRYTADSYLMERLRELGVI
>Mature_279_residues
MKLETIDYRAENSAQQFVESLRETGFGVLSNHPINKRLVEDIYTEWQAFFNSEAKNNYMFKKETHDGFFPASISETAKGN
TVKDIKEYYHVYPWGRIPDSLRANILAYYDKANALAAELLEWIETYSPEDVKAKFSIPLPEMIANSQKTLLRILHYPPMT
GSEEMGAIRAAAHEDINLITVLPAANEPGLQVKAKDGTWLDVPSDFGNIIINIGDMLQEASGGYFPSTSHRVINPEGTDK
TKSRISLPLFLHPNPAVVLSDRYTADSYLMERLRELGVI

Specific function: Unknown

COG id: COG3491

COG function: function code R; Isopenicillin N synthase and related dioxygenases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Drosophila melanogaster, GI28572124, Length=265, Percent_Identity=25.6603773584906, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.21.3.1

Molecular weight: Translated: 31475; Mature: 31475

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLETIDYRAENSAQQFVESLRETGFGVLSNHPINKRLVEDIYTEWQAFFNSEAKNNYMF
CCCCCCCCCCCCHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEEE
KKETHDGFFPASISETAKGNTVKDIKEYYHVYPWGRIPDSLRANILAYYDKANALAAELL
EECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHCCCEEECCHHHHHHHHH
EWIETYSPEDVKAKFSIPLPEMIANSQKTLLRILHYPPMTGSEEMGAIRAAAHEDINLIT
HHHHHCCCCCCEEEECCCCHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEE
VLPAANEPGLQVKAKDGTWLDVPSDFGNIIINIGDMLQEASGGYFPSTSHRVINPEGTDK
EEECCCCCCCEEEECCCCEEECCCCCCCEEEEHHHHHHHHCCCCCCCCCCEEECCCCCCC
TKSRISLPLFLHPNPAVVLSDRYTADSYLMERLRELGVI
CHHHEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLETIDYRAENSAQQFVESLRETGFGVLSNHPINKRLVEDIYTEWQAFFNSEAKNNYMF
CCCCCCCCCCCCHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEEE
KKETHDGFFPASISETAKGNTVKDIKEYYHVYPWGRIPDSLRANILAYYDKANALAAELL
EECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHCCCEEECCHHHHHHHHH
EWIETYSPEDVKAKFSIPLPEMIANSQKTLLRILHYPPMTGSEEMGAIRAAAHEDINLIT
HHHHHCCCCCCEEEECCCCHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEE
VLPAANEPGLQVKAKDGTWLDVPSDFGNIIINIGDMLQEASGGYFPSTSHRVINPEGTDK
EEECCCCCCCEEEECCCCEEECCCCCCCEEEEHHHHHHHHCCCCCCCCCCEEECCCCCCC
TKSRISLPLFLHPNPAVVLSDRYTADSYLMERLRELGVI
CHHHEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine; O2

Specific reaction: N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 = isopenicillin N + 2 H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA