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The map label for this gene is hemB [H]

Identifier: 120599074

GI number: 120599074

Start: 2616985

End: 2617968

Strand: Direct

Name: hemB [H]

Synonym: Sputw3181_2270

Alternate gene names: 120599074

Gene position: 2616985-2617968 (Clockwise)

Preceding gene: 120599066

Following gene: 120599076

Centisome position: 55.58

GC content: 49.7

Gene sequence:

>984_bases
ATGTTAAACACGCCTCCATTGCGTCGCCTAAGACGCTTACGTAGCTCAGAAGCGATGCGCGATCTGCTGCGCGAAACCCA
AATATCATTATCAGATTTAATCCACCCGCTATTTATTGAAGAACAGATCAATCATGCGATACCCATTTCGACTCTTCCTG
GAATTAGTCGTCTTCCTGAAAGCGCATTAGCCGATGAGGTTCGTGAACTTTATGCACTCGGTATTCGCTATGTGATGCCC
TTTGGAATTTCCCATACAAAAGATGATAAAGGCAGCGACACTTGGGATGACAATGGGTTACTCGCCCGTATGGTGCGCGC
AATTAAAACCGCAGTGCCCGAGATGATGGTGATCCCCGACATTTGCTTTTGTGAATACACAGATCACGGCCATTGCGGCG
TGGTGCACAATAATGAAGTCTGCAACGATCTCACCGTCGAAAATTTAGTTAAGCAATCGGTGACGGCCGCCAAAGCTGGC
GCCGATATGTTAGCGCCATCGGCCATGATGGACGGACAAGTGAAAGCCATTCGCCAAGGGCTCGATGCCGCAGGTTTTGA
ACATGTGGCGATTCTGGCCCACTCGGCAAAATTCGCCTCTTCTTTTTACGGTCCTTTTAGAGCGGCGGTAGATTGCGAAC
TCAGCGGAAATCGCAAGGGATATCAACTGGATTATGCTAATGGTCGTCAAGCCCTGTTAGAAGCCTTATTAGATGAAGCC
GAAGGCGCCGATATCCTGATGGTTAAACCCGGCACGCCCTACTTGGATGTGTTAAGCCGGTTAAGACAAGAAACCCACCT
ACCACTCGCCGCCTATCAAGTCGGCGGCGAATATGCTGGCATCAAGTTTGCTGCCCTTGCAGGCGCCTTGGATGAGCGTG
CCGTGGTGACAGAAACGTTTATCGGCTTAAAGCGGGCGGGTGCAGATTTGATTGTCAGCTATTACACCAAACAGTATGCC
CAGTGGTTAGCTGAAAACCATTAA

Upstream 100 bases:

>100_bases
CGATAAAATGAGGAAACTTTTTAGCTTATATCTGTCAGAGCTAACACCTGAACCTAGATTGAACCACAGTATTACGCTCC
ACCTTCGATAAGGAAATGTC

Downstream 100 bases:

>100_bases
TGACGGTGTTTTCACTTAACAACTAACAAAACGCCACCTTAATTGGGTGGCGTTTTTATTGCTTTAGCCTTACATGTCTT
GAGGAATGGCGTAAATTAAG

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MLNTPPLRRLRRLRSSEAMRDLLRETQISLSDLIHPLFIEEQINHAIPISTLPGISRLPESALADEVRELYALGIRYVMP
FGISHTKDDKGSDTWDDNGLLARMVRAIKTAVPEMMVIPDICFCEYTDHGHCGVVHNNEVCNDLTVENLVKQSVTAAKAG
ADMLAPSAMMDGQVKAIRQGLDAAGFEHVAILAHSAKFASSFYGPFRAAVDCELSGNRKGYQLDYANGRQALLEALLDEA
EGADILMVKPGTPYLDVLSRLRQETHLPLAAYQVGGEYAGIKFAALAGALDERAVVTETFIGLKRAGADLIVSYYTKQYA
QWLAENH

Sequences:

>Translated_327_residues
MLNTPPLRRLRRLRSSEAMRDLLRETQISLSDLIHPLFIEEQINHAIPISTLPGISRLPESALADEVRELYALGIRYVMP
FGISHTKDDKGSDTWDDNGLLARMVRAIKTAVPEMMVIPDICFCEYTDHGHCGVVHNNEVCNDLTVENLVKQSVTAAKAG
ADMLAPSAMMDGQVKAIRQGLDAAGFEHVAILAHSAKFASSFYGPFRAAVDCELSGNRKGYQLDYANGRQALLEALLDEA
EGADILMVKPGTPYLDVLSRLRQETHLPLAAYQVGGEYAGIKFAALAGALDERAVVTETFIGLKRAGADLIVSYYTKQYA
QWLAENH
>Mature_327_residues
MLNTPPLRRLRRLRSSEAMRDLLRETQISLSDLIHPLFIEEQINHAIPISTLPGISRLPESALADEVRELYALGIRYVMP
FGISHTKDDKGSDTWDDNGLLARMVRAIKTAVPEMMVIPDICFCEYTDHGHCGVVHNNEVCNDLTVENLVKQSVTAAKAG
ADMLAPSAMMDGQVKAIRQGLDAAGFEHVAILAHSAKFASSFYGPFRAAVDCELSGNRKGYQLDYANGRQALLEALLDEA
EGADILMVKPGTPYLDVLSRLRQETHLPLAAYQVGGEYAGIKFAALAGALDERAVVTETFIGLKRAGADLIVSYYTKQYA
QWLAENH

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=307, Percent_Identity=40.0651465798046, Blast_Score=224, Evalue=9e-59,
Organism=Escherichia coli, GI87081728, Length=318, Percent_Identity=57.2327044025157, Blast_Score=364, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6321398, Length=317, Percent_Identity=39.4321766561514, Blast_Score=209, Evalue=6e-55,
Organism=Drosophila melanogaster, GI21358291, Length=294, Percent_Identity=39.4557823129252, Blast_Score=210, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 35944; Mature: 35944

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNTPPLRRLRRLRSSEAMRDLLRETQISLSDLIHPLFIEEQINHAIPISTLPGISRLPE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCH
SALADEVRELYALGIRYVMPFGISHTKDDKGSDTWDDNGLLARMVRAIKTAVPEMMVIPD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHCCC
ICFCEYTDHGHCGVVHNNEVCNDLTVENLVKQSVTAAKAGADMLAPSAMMDGQVKAIRQG
EEEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHCCHHHHHHHC
LDAAGFEHVAILAHSAKFASSFYGPFRAAVDCELSGNRKGYQLDYANGRQALLEALLDEA
CCCCCCHHHHHHHHHHHHHHHHCCCHHHEEEEEECCCCCCEEEECCCCHHHHHHHHHHHC
EGADILMVKPGTPYLDVLSRLRQETHLPLAAYQVGGEYAGIKFAALAGALDERAVVTETF
CCCCEEEECCCCCHHHHHHHHHHHHCCCHHEECCCCCCCCCHHHHHHHHHCCHHHHHHHH
IGLKRAGADLIVSYYTKQYAQWLAENH
HHHHHCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLNTPPLRRLRRLRSSEAMRDLLRETQISLSDLIHPLFIEEQINHAIPISTLPGISRLPE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCH
SALADEVRELYALGIRYVMPFGISHTKDDKGSDTWDDNGLLARMVRAIKTAVPEMMVIPD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHCCC
ICFCEYTDHGHCGVVHNNEVCNDLTVENLVKQSVTAAKAGADMLAPSAMMDGQVKAIRQG
EEEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHCCHHHHHHHC
LDAAGFEHVAILAHSAKFASSFYGPFRAAVDCELSGNRKGYQLDYANGRQALLEALLDEA
CCCCCCHHHHHHHHHHHHHHHHCCCHHHEEEEEECCCCCCEEEECCCCHHHHHHHHHHHC
EGADILMVKPGTPYLDVLSRLRQETHLPLAAYQVGGEYAGIKFAALAGALDERAVVTETF
CCCCEEEECCCCCHHHHHHHHHHHHCCCHHEECCCCCCCCCHHHHHHHHHCCHHHHHHHH
IGLKRAGADLIVSYYTKQYAQWLAENH
HHHHHCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2656410; 2464127; 9278503; 8439296; 10194344; 11444968; 11909869 [H]