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The map label for this gene is frmA [H]

Identifier: 120598651

GI number: 120598651

Start: 2115868

End: 2117007

Strand: Direct

Name: frmA [H]

Synonym: Sputw3181_1835

Alternate gene names: 120598651

Gene position: 2115868-2117007 (Clockwise)

Preceding gene: 120598649

Following gene: 120598652

Centisome position: 44.94

GC content: 50.26

Gene sequence:

>1140_bases
ATGTCGAACGAAAAACCGCAGTTTATTAAATCAAAGGCCGCTGTTGCTTGGGGACCAGGACAACCACTTAAGATTGAAGA
AGTGGATGTGATGTTACCTAAAGCGGGCGAAGTCTTAGTGCGCATTGTGGCTACAGGCGTGTGTCACACAGATGCGTTTA
CCCTGAGCGGTGATGATCCAGAAGGGGTATTCCCGGCGATTCTAGGCCACGAAGGCGGCGGTATCGTTGAGCAAGTGGGC
GAGGGTGTGACCAGCGTACAAGTGGGCGATCATGTGATCCCACTTTATACCCCAGAGTGCGGCGAATGTAAGTTTTGTCT
ATCGGGCAAAACTAACTTGTGCCAAAAAATTCGTGCGACCCAAGGTAAAGGCTTAATGCCCGATGGCACCACGCGTTTTT
ATCTTGATGGCAAGCCTATTTTCCATTACATGGGCTGCTCGACTTTCTCTGAATATACCGTCTTGCCTGAGATCTCATTG
GCTAAAGTCAATAAGAGCGCGCCATTAGAAGAAATTTGTCTGTTAGGTTGTGGCGTAACCACAGGCATGGGCGCTGTGAT
GAACACAGCCAAAGTGGAAGAAGGTGCGACCGTGGCGATTTTCGGCCTCGGCGGTATCGGTTTATCGGCGATTATCGGTG
CCACTATGGCAAAAGCCAGTCGCATTATCGCGATTGATATCAACGAATCTAAGTTCGAGTTAGCCAAAAAACTCGGCGCG
ACCGATTGCATCAACCCAAAATTATTGGATAAGCCCATCCAAGAGGTGATCGTTGAGATGACCGACGGTGGCGTGGATTA
CTCATTCGAGTGTATCGGCAACGTCAACGTGATGCGCAGCGCCTTAGAGTGTTGTCATAAGGGCTGGGGCGAGTCGGTGA
TCATAGGTGTTGCCGGTGCTGGACAAGAGATTTCAACCCGTCCATTCCAGCTTGTGACGGGCCGCGTTTGGCGCGGTTCT
GCCTTTGGCGGGGTGAAAGGGCGTTCTGAACTGCCACACATCGTTGAGCGTTACTTAGCGGGCGAATTTAAGCTCGATGA
TTTTATCACCCATACTATGGGGCTTGAGCAAATCAATGAAGCATTCGAGCTGATGCACCAAGGTAAGAGCATCCGCAGCG
TGATCCATTGTGATAAATAA

Upstream 100 bases:

>100_bases
TCAGTAGCATCAGTGCAATGGGCGCAAGAGTTTGCTTGGCCTGAATTGAATTATGATACACCAATGACTTACTCCTATTA
CCTATTCAAGGGACGTAACA

Downstream 100 bases:

>100_bases
GCGGTTTCGCATTGTAAATGGATGCTTCAGCTTGATGTGACAAACCGGCAGACAGGTGCAAATCTGTTTGCTGTGTTGCC
AACTGAGCAGACGATCAGCG

Product: alcohol dehydrogenase

Products: NA

Alternate protein names: Alcohol dehydrogenase class-3; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH [H]

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MSNEKPQFIKSKAAVAWGPGQPLKIEEVDVMLPKAGEVLVRIVATGVCHTDAFTLSGDDPEGVFPAILGHEGGGIVEQVG
EGVTSVQVGDHVIPLYTPECGECKFCLSGKTNLCQKIRATQGKGLMPDGTTRFYLDGKPIFHYMGCSTFSEYTVLPEISL
AKVNKSAPLEEICLLGCGVTTGMGAVMNTAKVEEGATVAIFGLGGIGLSAIIGATMAKASRIIAIDINESKFELAKKLGA
TDCINPKLLDKPIQEVIVEMTDGGVDYSFECIGNVNVMRSALECCHKGWGESVIIGVAGAGQEISTRPFQLVTGRVWRGS
AFGGVKGRSELPHIVERYLAGEFKLDDFITHTMGLEQINEAFELMHQGKSIRSVIHCDK

Sequences:

>Translated_379_residues
MSNEKPQFIKSKAAVAWGPGQPLKIEEVDVMLPKAGEVLVRIVATGVCHTDAFTLSGDDPEGVFPAILGHEGGGIVEQVG
EGVTSVQVGDHVIPLYTPECGECKFCLSGKTNLCQKIRATQGKGLMPDGTTRFYLDGKPIFHYMGCSTFSEYTVLPEISL
AKVNKSAPLEEICLLGCGVTTGMGAVMNTAKVEEGATVAIFGLGGIGLSAIIGATMAKASRIIAIDINESKFELAKKLGA
TDCINPKLLDKPIQEVIVEMTDGGVDYSFECIGNVNVMRSALECCHKGWGESVIIGVAGAGQEISTRPFQLVTGRVWRGS
AFGGVKGRSELPHIVERYLAGEFKLDDFITHTMGLEQINEAFELMHQGKSIRSVIHCDK
>Mature_378_residues
SNEKPQFIKSKAAVAWGPGQPLKIEEVDVMLPKAGEVLVRIVATGVCHTDAFTLSGDDPEGVFPAILGHEGGGIVEQVGE
GVTSVQVGDHVIPLYTPECGECKFCLSGKTNLCQKIRATQGKGLMPDGTTRFYLDGKPIFHYMGCSTFSEYTVLPEISLA
KVNKSAPLEEICLLGCGVTTGMGAVMNTAKVEEGATVAIFGLGGIGLSAIIGATMAKASRIIAIDINESKFELAKKLGAT
DCINPKLLDKPIQEVIVEMTDGGVDYSFECIGNVNVMRSALECCHKGWGESVIIGVAGAGQEISTRPFQLVTGRVWRGSA
FGGVKGRSELPHIVERYLAGEFKLDDFITHTMGLEQINEAFELMHQGKSIRSVIHCDK

Specific function: Has High Formaldehyde Dehydrogenase Activity In The Presence Of Glutathione And Catalyzes The Oxidation Of Normal Alcohols In A Reaction That Is Not Gsh-Dependent. In Addition, Hemithiolacetals Other Than Those Formed From Gsh, Including Omega-Thiol Fatty

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily [H]

Homologues:

Organism=Homo sapiens, GI71565154, Length=375, Percent_Identity=65.6, Blast_Score=511, Evalue=1e-145,
Organism=Homo sapiens, GI34577061, Length=371, Percent_Identity=52.5606469002695, Blast_Score=409, Evalue=1e-114,
Organism=Homo sapiens, GI71565152, Length=375, Percent_Identity=52, Blast_Score=399, Evalue=1e-111,
Organism=Homo sapiens, GI4501929, Length=371, Percent_Identity=51.7520215633423, Blast_Score=397, Evalue=1e-111,
Organism=Homo sapiens, GI4501933, Length=371, Percent_Identity=51.2129380053908, Blast_Score=396, Evalue=1e-110,
Organism=Homo sapiens, GI156523966, Length=369, Percent_Identity=49.3224932249323, Blast_Score=384, Evalue=1e-107,
Organism=Homo sapiens, GI4501939, Length=363, Percent_Identity=49.5867768595041, Blast_Score=379, Evalue=1e-105,
Organism=Homo sapiens, GI262073058, Length=367, Percent_Identity=49.8637602179836, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI71743840, Length=369, Percent_Identity=49.5934959349593, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI156627571, Length=376, Percent_Identity=24.7340425531915, Blast_Score=76, Evalue=6e-14,
Organism=Escherichia coli, GI1786552, Length=367, Percent_Identity=69.7547683923706, Blast_Score=528, Evalue=1e-151,
Organism=Escherichia coli, GI1787863, Length=369, Percent_Identity=26.5582655826558, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1790045, Length=372, Percent_Identity=27.4193548387097, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI87082125, Length=375, Percent_Identity=25.8666666666667, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1788073, Length=361, Percent_Identity=26.5927977839335, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI87081918, Length=228, Percent_Identity=34.6491228070175, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1786825, Length=274, Percent_Identity=28.1021897810219, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1788075, Length=367, Percent_Identity=26.4305177111717, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1788407, Length=225, Percent_Identity=30.2222222222222, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1786518, Length=372, Percent_Identity=26.0752688172043, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI226510992, Length=353, Percent_Identity=22.0963172804533, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25146526, Length=383, Percent_Identity=60.0522193211488, Blast_Score=484, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI71997431, Length=385, Percent_Identity=59.7402597402597, Blast_Score=479, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI17565904, Length=339, Percent_Identity=60.4719764011799, Blast_Score=441, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI17562584, Length=360, Percent_Identity=27.5, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17562582, Length=380, Percent_Identity=27.6315789473684, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71988145, Length=353, Percent_Identity=26.628895184136, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6320033, Length=375, Percent_Identity=59.4666666666667, Blast_Score=471, Evalue=1e-134,
Organism=Saccharomyces cerevisiae, GI6319621, Length=378, Percent_Identity=29.1005291005291, Blast_Score=97, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6323099, Length=379, Percent_Identity=29.023746701847, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323729, Length=366, Percent_Identity=28.6885245901639, Blast_Score=93, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6324486, Length=364, Percent_Identity=26.9230769230769, Blast_Score=90, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6323961, Length=364, Percent_Identity=26.6483516483516, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6319258, Length=266, Percent_Identity=27.0676691729323, Blast_Score=73, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6322619, Length=362, Percent_Identity=26.5193370165746, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17737895, Length=377, Percent_Identity=63.9257294429708, Blast_Score=503, Evalue=1e-143,
Organism=Drosophila melanogaster, GI17137530, Length=348, Percent_Identity=27.5862068965517, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI17737897, Length=373, Percent_Identity=25.4691689008043, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014183
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.284; =1.1.1.1 [H]

Molecular weight: Translated: 40468; Mature: 40337

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNEKPQFIKSKAAVAWGPGQPLKIEEVDVMLPKAGEVLVRIVATGVCHTDAFTLSGDDP
CCCCCCCHHHHCCEEEECCCCCCEEEEEEEECCCHHHHHHHHHHHCCCCCCEEEECCCCC
EGVFPAILGHEGGGIVEQVGEGVTSVQVGDHVIPLYTPECGECKFCLSGKTNLCQKIRAT
CCCCHHHCCCCCCCHHHHHCCCCEEEEECCEEEEEECCCCCCEEEEECCCHHHHHHHHHC
QGKGLMPDGTTRFYLDGKPIFHYMGCSTFSEYTVLPEISLAKVNKSAPLEEICLLGCGVT
CCCCCCCCCCEEEEECCCCEEEEECCCCCCCEEECCCCHHHHCCCCCCHHHHHHHHCCHH
TGMGAVMNTAKVEEGATVAIFGLGGIGLSAIIGATMAKASRIIAIDINESKFELAKKLGA
HHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHCCC
TDCINPKLLDKPIQEVIVEMTDGGVDYSFECIGNVNVMRSALECCHKGWGESVIIGVAGA
CCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEECC
GQEISTRPFQLVTGRVWRGSAFGGVKGRSELPHIVERYLAGEFKLDDFITHTMGLEQINE
CCCCCCCCEEEEECEEECCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHCHHHHHH
AFELMHQGKSIRSVIHCDK
HHHHHHCCHHHHHHHCCCC
>Mature Secondary Structure 
SNEKPQFIKSKAAVAWGPGQPLKIEEVDVMLPKAGEVLVRIVATGVCHTDAFTLSGDDP
CCCCCCHHHHCCEEEECCCCCCEEEEEEEECCCHHHHHHHHHHHCCCCCCEEEECCCCC
EGVFPAILGHEGGGIVEQVGEGVTSVQVGDHVIPLYTPECGECKFCLSGKTNLCQKIRAT
CCCCHHHCCCCCCCHHHHHCCCCEEEEECCEEEEEECCCCCCEEEEECCCHHHHHHHHHC
QGKGLMPDGTTRFYLDGKPIFHYMGCSTFSEYTVLPEISLAKVNKSAPLEEICLLGCGVT
CCCCCCCCCCEEEEECCCCEEEEECCCCCCCEEECCCCHHHHCCCCCCHHHHHHHHCCHH
TGMGAVMNTAKVEEGATVAIFGLGGIGLSAIIGATMAKASRIIAIDINESKFELAKKLGA
HHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHCCC
TDCINPKLLDKPIQEVIVEMTDGGVDYSFECIGNVNVMRSALECCHKGWGESVIIGVAGA
CCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEECC
GQEISTRPFQLVTGRVWRGSAFGGVKGRSELPHIVERYLAGEFKLDDFITHTMGLEQINE
CCCCCCCCEEEEECEEECCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHCHHHHHH
AFELMHQGKSIRSVIHCDK
HHHHHHCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]