Click here to switch to the map view.

The map label for this gene is puuB [H]

Identifier: 120598649

GI number: 120598649

Start: 2113472

End: 2114761

Strand: Direct

Name: puuB [H]

Synonym: Sputw3181_1833

Alternate gene names: 120598649

Gene position: 2113472-2114761 (Clockwise)

Preceding gene: 120598635

Following gene: 120598651

Centisome position: 44.89

GC content: 51.47

Gene sequence:

>1290_bases
ATGCCAGCAGAGCGTTGCGCCTCCTACTACAATACCACCATTGACCAAGAGTCAGATTATCCTGCGCTTGAAGGCGACAT
TCGAGTCGATGTGGCGATTGTCGGTGGCGGTTTTACTGGCGTGGCAACGGCGTTGGAATTATCTGAGAAGGGCTACAAAG
TGGCGCTTATTGAGGCCAATAAAATTGCGTGGGGCGCGACCGGACGCAACGGCGGGCAGGTGACGGGCAGTTTGTCCGGC
GATGTTGCGATGACAAAGCAGCTGCGCTGTCAAATCGGCAATGAAGCTGAAGATTATGTGTGGAATATGCGTTGGCGCGG
CCACGATATAATTAAAAATCGGGTGGCCAAGTACGGCATAGACTGCGATTTAAAGTTTGGTCATATCCAAACCGCTTACC
AGCCAAAGCACATGCAAGAGCTTAGGGCTATGCATGATGAGGCGCAGCGCCGCGGCATGGGTGAATTTATGACGCTGGTA
TCCGCCGATGAAATGCCTGCCTATTTAGGTTCGCCCTTGTATCACGGCGGCTTAGTCAATCGGCGCAATATGCATTTGCA
CTCAGTTAATTTATGCCTGGGTGAAGCCCGCGCCGCAGCAGGTCTTGGGGCGTTAATCTTTGAACACTCGCCCGTACTTG
ATATTGAAGAAGGCGATGTGGCGACTGTTGTCACTACCAAGGGAAAAGTGCGCGCCAACAGTGTGCTGATCGCCGGTAAT
GCTTACCATAAACTCGCCCGTCCAAAACTGCGCGGTATGTTATTTCCGGCGTCCTTAGGCAATTGCGCCACTGCGATTCT
GCCCGATGATATCGCTTTGCAAATTAATCCGCAGGATTTAGCCGTGTACGATTGCCGCTTTGTGTTGGATTACTATCGTC
TGACCGCAGATAAACGGTTGATGTTCGGCGGTGGCACTAATTACAGCGGCCGCGATCCTAAAAATGTGGCGGCGGAATTA
CGGCCCGCGATTGAGCGTACGTTTCCACAATTAAAGGGCGTTGATATCGAATTTGCCTGGGCGGGAATGGCAGGAATTGT
GATTAACCGTATCCCGCAGCTCGGCAAAGTGTCGCCTAATGTATTCTATTGCCAAGGATATTCTGGGCACGGAGTGGCGA
CATCGCACATTATGGCCGAGATCATGGCCAATGCGATGGATGGGCAATTACATGAATTTGATTTGTTTGCCGCTATGCGC
CATATCCGCATTCCGTTAAATGAATGGTTCGGCAATCAAGCGCTGGCGTTAGGCATGTTGTATTACACTTTGCGGGAAAA
CTGGCGCTAG

Upstream 100 bases:

>100_bases
AACAGAGTGTCCAGAAGAACTGAGTGTCCAGAACTAAGACTCAACGCTCACTTTCAGCCATCAACTTTCAACAGTTAACT
TTCAACATCAAGGAATGACC

Downstream 100 bases:

>100_bases
TTTGCGCCGTTTTGTCTGCTACCACCTACTAATCAATCTGCTCAAACAGTATTTAATCCTGCTCGGCGAGTTTTTCGGCA
AGGTAATCGACCAATACACG

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MPAERCASYYNTTIDQESDYPALEGDIRVDVAIVGGGFTGVATALELSEKGYKVALIEANKIAWGATGRNGGQVTGSLSG
DVAMTKQLRCQIGNEAEDYVWNMRWRGHDIIKNRVAKYGIDCDLKFGHIQTAYQPKHMQELRAMHDEAQRRGMGEFMTLV
SADEMPAYLGSPLYHGGLVNRRNMHLHSVNLCLGEARAAAGLGALIFEHSPVLDIEEGDVATVVTTKGKVRANSVLIAGN
AYHKLARPKLRGMLFPASLGNCATAILPDDIALQINPQDLAVYDCRFVLDYYRLTADKRLMFGGGTNYSGRDPKNVAAEL
RPAIERTFPQLKGVDIEFAWAGMAGIVINRIPQLGKVSPNVFYCQGYSGHGVATSHIMAEIMANAMDGQLHEFDLFAAMR
HIRIPLNEWFGNQALALGMLYYTLRENWR

Sequences:

>Translated_429_residues
MPAERCASYYNTTIDQESDYPALEGDIRVDVAIVGGGFTGVATALELSEKGYKVALIEANKIAWGATGRNGGQVTGSLSG
DVAMTKQLRCQIGNEAEDYVWNMRWRGHDIIKNRVAKYGIDCDLKFGHIQTAYQPKHMQELRAMHDEAQRRGMGEFMTLV
SADEMPAYLGSPLYHGGLVNRRNMHLHSVNLCLGEARAAAGLGALIFEHSPVLDIEEGDVATVVTTKGKVRANSVLIAGN
AYHKLARPKLRGMLFPASLGNCATAILPDDIALQINPQDLAVYDCRFVLDYYRLTADKRLMFGGGTNYSGRDPKNVAAEL
RPAIERTFPQLKGVDIEFAWAGMAGIVINRIPQLGKVSPNVFYCQGYSGHGVATSHIMAEIMANAMDGQLHEFDLFAAMR
HIRIPLNEWFGNQALALGMLYYTLRENWR
>Mature_428_residues
PAERCASYYNTTIDQESDYPALEGDIRVDVAIVGGGFTGVATALELSEKGYKVALIEANKIAWGATGRNGGQVTGSLSGD
VAMTKQLRCQIGNEAEDYVWNMRWRGHDIIKNRVAKYGIDCDLKFGHIQTAYQPKHMQELRAMHDEAQRRGMGEFMTLVS
ADEMPAYLGSPLYHGGLVNRRNMHLHSVNLCLGEARAAAGLGALIFEHSPVLDIEEGDVATVVTTKGKVRANSVLIAGNA
YHKLARPKLRGMLFPASLGNCATAILPDDIALQINPQDLAVYDCRFVLDYYRLTADKRLMFGGGTNYSGRDPKNVAAELR
PAIERTFPQLKGVDIEFAWAGMAGIVINRIPQLGKVSPNVFYCQGYSGHGVATSHIMAEIMANAMDGQLHEFDLFAAMRH
IRIPLNEWFGNQALALGMLYYTLRENWR

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=425, Percent_Identity=36.2352941176471, Blast_Score=291, Evalue=4e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 47289; Mature: 47158

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAERCASYYNTTIDQESDYPALEGDIRVDVAIVGGGFTGVATALELSEKGYKVALIEAN
CCHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHCCCCCEEEEEEEC
KIAWGATGRNGGQVTGSLSGDVAMTKQLRCQIGNEAEDYVWNMRWRGHDIIKNRVAKYGI
EEEECCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCHHEEEEEEECCHHHHHHHHHHCCC
DCDLKFGHIQTAYQPKHMQELRAMHDEAQRRGMGEFMTLVSADEMPAYLGSPLYHGGLVN
CEEEEECCEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHCCCCCCCCCCC
RRNMHLHSVNLCLGEARAAAGLGALIFEHSPVLDIEEGDVATVVTTKGKVRANSVLIAGN
CCCCEEEEEEEEHHHHHHHCCCEEEEECCCCEEEECCCCEEEEEEECCCEEECEEEEECC
AYHKLARPKLRGMLFPASLGNCATAILPDDIALQINPQDLAVYDCRFVLDYYRLTADKRL
HHHHHCCHHHHCEECCCCCCCCEEEECCCCEEEEECCCCEEEEHHHHHHHHHHHCCCCEE
MFGGGTNYSGRDPKNVAAELRPAIERTFPQLKGVDIEFAWAGMAGIVINRIPQLGKVSPN
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHCCCCCCCCCCC
VFYCQGYSGHGVATSHIMAEIMANAMDGQLHEFDLFAAMRHIRIPLNEWFGNQALALGML
EEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHH
YYTLRENWR
HHHHHHCCC
>Mature Secondary Structure 
PAERCASYYNTTIDQESDYPALEGDIRVDVAIVGGGFTGVATALELSEKGYKVALIEAN
CHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHCCCCCEEEEEEEC
KIAWGATGRNGGQVTGSLSGDVAMTKQLRCQIGNEAEDYVWNMRWRGHDIIKNRVAKYGI
EEEECCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCHHEEEEEEECCHHHHHHHHHHCCC
DCDLKFGHIQTAYQPKHMQELRAMHDEAQRRGMGEFMTLVSADEMPAYLGSPLYHGGLVN
CEEEEECCEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHCCCCCCCCCCC
RRNMHLHSVNLCLGEARAAAGLGALIFEHSPVLDIEEGDVATVVTTKGKVRANSVLIAGN
CCCCEEEEEEEEHHHHHHHCCCEEEEECCCCEEEECCCCEEEEEEECCCEEECEEEEECC
AYHKLARPKLRGMLFPASLGNCATAILPDDIALQINPQDLAVYDCRFVLDYYRLTADKRL
HHHHHCCHHHHCEECCCCCCCCEEEECCCCEEEEECCCCEEEEHHHHHHHHHHHCCCCEE
MFGGGTNYSGRDPKNVAAELRPAIERTFPQLKGVDIEFAWAGMAGIVINRIPQLGKVSPN
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHCCCCCCCCCCC
VFYCQGYSGHGVATSHIMAEIMANAMDGQLHEFDLFAAMRHIRIPLNEWFGNQALALGML
EEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHH
YYTLRENWR
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]