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The map label for this gene is truD [H]

Identifier: 120598082

GI number: 120598082

Start: 1431404

End: 1432486

Strand: Direct

Name: truD [H]

Synonym: Sputw3181_1259

Alternate gene names: 120598082

Gene position: 1431404-1432486 (Clockwise)

Preceding gene: 120598081

Following gene: 120598083

Centisome position: 30.4

GC content: 44.97

Gene sequence:

>1083_bases
ATGAGCGAACTGCATTACTTATATGGCAAACCACTCGGCACTGCCGATTTACGTACTCAAAATAGCGATTTTATCGTGAA
AGAGATTTTGCCCTTTAGCCCAACAGGAGAAGGGGAACATCATCTCTTACATATCCGTAAAGACGGGCTGAATACGGTTC
AAGTGGCAGAAATGATTGCCAAGTTTGCAAAGGTGCATCCAAAGGAAGTGACCTATGCGGGCCAGAAAGATAAAAATGCC
ATTACTGAACAATGGTTTGGTGTACGCATTCCGGGTAAAGAAACTCCCGCTTGGGCAGAACTTAACAGTGAACGATTAAC
GCTTCTCTCTAGTAGTCGCCATAGCAAAAAATTACGCATCGGTGCACTACTGGGTAATCGCTTTATTTTGACTTTGCGTA
ATGTCACTCACGCTGAAGATATCATTAGCCGGATTGAGAAAGTGAGTCAAATCGGTGTCCCCAATTATTTTGGTGAGCAG
CGTTTTGGCCATGATGGTAAAAATCTCGTCATGGGACGCCAAATGTTGGCGGGTAAAAAAGTGAAAGACAGAAACAAACG
CAGCATGTATTTATCTGCTGTGCGCTCAAACTTGTTTAATACCGTCGTGTCATACCGCTTAGCCAACCATGGTACTAAAC
CTTTAGCGGGTGATTGTGTGATGTTGGCGGGTAGCAAAAGTTTCTTCGTGACACCCGAATGGGATCTGGTGGTGTTGAAA
CGTCTGATTGAAAAAGATATTCAACTATCAGCACCTTTGTGGGGTCGTGGCATGATGTTGCCTCAAGGCGAAGCCGCGGC
GATGGAAACCTTAGCGATGAGTGAGCTTAGCGATGACTGCTATGGTTTGGAACATGCAGGATTGGAGCAGGAACGTCGTC
CATTACTGCTTGAGCCTCAAGGGCTTAAATACGAAATAACAGAAGATAGTTTAGTGTTAGAGTTTATTTTACCCGCGGGT
AGCTTTGCGACATCTCTACTTAGGGAACTGGTTAATTATCAGGATGTAAAAGAGCAGCAGTGGCAAGCAACAGTTACGTC
AGAGGCTAACGCTGTTAACGGTAGTGATGACGCTGCATCATGA

Upstream 100 bases:

>100_bases
TGAAAAGTTAGGGTTTACAGGACGTAAAGAAGGCATTGCGGTAGAAGCAGTTGTGTTGATGAGCCGTAAACAAGATTAAT
TAACGAGATAGATAAGTCCC

Downstream 100 bases:

>100_bases
TCCGCATTCTTGTTAGTAATGATGATGGTGTAAATGCTCCTGGCATCAAAGCCTTAACAGAAGCACTCGTTGAAATAGCC
AACGTGATGACAGTCGCGCC

Product: pseudouridylate synthase

Products: NA

Alternate protein names: tRNA pseudouridylate synthase D; tRNA-uridine isomerase D [H]

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MSELHYLYGKPLGTADLRTQNSDFIVKEILPFSPTGEGEHHLLHIRKDGLNTVQVAEMIAKFAKVHPKEVTYAGQKDKNA
ITEQWFGVRIPGKETPAWAELNSERLTLLSSSRHSKKLRIGALLGNRFILTLRNVTHAEDIISRIEKVSQIGVPNYFGEQ
RFGHDGKNLVMGRQMLAGKKVKDRNKRSMYLSAVRSNLFNTVVSYRLANHGTKPLAGDCVMLAGSKSFFVTPEWDLVVLK
RLIEKDIQLSAPLWGRGMMLPQGEAAAMETLAMSELSDDCYGLEHAGLEQERRPLLLEPQGLKYEITEDSLVLEFILPAG
SFATSLLRELVNYQDVKEQQWQATVTSEANAVNGSDDAAS

Sequences:

>Translated_360_residues
MSELHYLYGKPLGTADLRTQNSDFIVKEILPFSPTGEGEHHLLHIRKDGLNTVQVAEMIAKFAKVHPKEVTYAGQKDKNA
ITEQWFGVRIPGKETPAWAELNSERLTLLSSSRHSKKLRIGALLGNRFILTLRNVTHAEDIISRIEKVSQIGVPNYFGEQ
RFGHDGKNLVMGRQMLAGKKVKDRNKRSMYLSAVRSNLFNTVVSYRLANHGTKPLAGDCVMLAGSKSFFVTPEWDLVVLK
RLIEKDIQLSAPLWGRGMMLPQGEAAAMETLAMSELSDDCYGLEHAGLEQERRPLLLEPQGLKYEITEDSLVLEFILPAG
SFATSLLRELVNYQDVKEQQWQATVTSEANAVNGSDDAAS
>Mature_359_residues
SELHYLYGKPLGTADLRTQNSDFIVKEILPFSPTGEGEHHLLHIRKDGLNTVQVAEMIAKFAKVHPKEVTYAGQKDKNAI
TEQWFGVRIPGKETPAWAELNSERLTLLSSSRHSKKLRIGALLGNRFILTLRNVTHAEDIISRIEKVSQIGVPNYFGEQR
FGHDGKNLVMGRQMLAGKKVKDRNKRSMYLSAVRSNLFNTVVSYRLANHGTKPLAGDCVMLAGSKSFFVTPEWDLVVLKR
LIEKDIQLSAPLWGRGMMLPQGEAAAMETLAMSELSDDCYGLEHAGLEQERRPLLLEPQGLKYEITEDSLVLEFILPAGS
FATSLLRELVNYQDVKEQQWQATVTSEANAVNGSDDAAS

Specific function: Responsible for synthesis of pseudouridine from uracil- 13 in transfer RNAs [H]

COG id: COG0585

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRUD domain [H]

Homologues:

Organism=Homo sapiens, GI148762985, Length=145, Percent_Identity=32.4137931034483, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI148762961, Length=145, Percent_Identity=32.4137931034483, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI148762948, Length=145, Percent_Identity=32.4137931034483, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI50727002, Length=134, Percent_Identity=32.089552238806, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1789102, Length=334, Percent_Identity=46.1077844311377, Blast_Score=269, Evalue=2e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001656
- InterPro:   IPR020119
- InterPro:   IPR011760 [H]

Pfam domain/function: PF01142 TruD [H]

EC number: NA

Molecular weight: Translated: 40193; Mature: 40061

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: PS50984 TRUD ; PS01268 UPF0024

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELHYLYGKPLGTADLRTQNSDFIVKEILPFSPTGEGEHHLLHIRKDGLNTVQVAEMIA
CCCCCCHHCCCCCCCCCCCCCCCEEHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHH
KFAKVHPKEVTYAGQKDKNAITEQWFGVRIPGKETPAWAELNSERLTLLSSSRHSKKLRI
HHHHCCCHHEEECCCCCCHHHHHHHCEEECCCCCCCCHHHCCCCCEEEECCCCCCCEEEE
GALLGNRFILTLRNVTHAEDIISRIEKVSQIGVPNYFGEQRFGHDGKNLVMGRQMLAGKK
EEEECCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCHHHHHHHHHCCCC
VKDRNKRSMYLSAVRSNLFNTVVSYRLANHGTKPLAGDCVMLAGSKSFFVTPEWDLVVLK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEEECCCHHHHHHH
RLIEKDIQLSAPLWGRGMMLPQGEAAAMETLAMSELSDDCYGLEHAGLEQERRPLLLEPQ
HHHHHHCEECCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHCCCCCCCCCHHCCCEEECCC
GLKYEITEDSLVLEFILPAGSFATSLLRELVNYQDVKEQQWQATVTSEANAVNGSDDAAS
CCEEEECCCCEEEEEECCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SELHYLYGKPLGTADLRTQNSDFIVKEILPFSPTGEGEHHLLHIRKDGLNTVQVAEMIA
CCCCCHHCCCCCCCCCCCCCCCEEHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHH
KFAKVHPKEVTYAGQKDKNAITEQWFGVRIPGKETPAWAELNSERLTLLSSSRHSKKLRI
HHHHCCCHHEEECCCCCCHHHHHHHCEEECCCCCCCCHHHCCCCCEEEECCCCCCCEEEE
GALLGNRFILTLRNVTHAEDIISRIEKVSQIGVPNYFGEQRFGHDGKNLVMGRQMLAGKK
EEEECCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCHHHHHHHHHCCCC
VKDRNKRSMYLSAVRSNLFNTVVSYRLANHGTKPLAGDCVMLAGSKSFFVTPEWDLVVLK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEEECCCHHHHHHH
RLIEKDIQLSAPLWGRGMMLPQGEAAAMETLAMSELSDDCYGLEHAGLEQERRPLLLEPQ
HHHHHHCEECCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHCCCCCCCCCHHCCCEEECCC
GLKYEITEDSLVLEFILPAGSFATSLLRELVNYQDVKEQQWQATVTSEANAVNGSDDAAS
CCEEEECCCCEEEEEECCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]