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The map label for this gene is ispF

Identifier: 120598081

GI number: 120598081

Start: 1430897

End: 1431382

Strand: Direct

Name: ispF

Synonym: Sputw3181_1258

Alternate gene names: 120598081

Gene position: 1430897-1431382 (Clockwise)

Preceding gene: 120598080

Following gene: 120598082

Centisome position: 30.39

GC content: 45.47

Gene sequence:

>486_bases
ATGAAAATCCGTATCGGTCATGGTTTCGATGTTCACAAATTTGGCGAAGTTCGGCCCTTAATATTGTGTGGTGTTGAAGT
ACCCTATGAGACTGGGCTCGTCGCACATTCCGATGGTGATGTGGTGTTGCATGCAGTGTCTGATGCCATTCTGGGAGCGA
TGGCTCTCGGAGATATAGGCAAACATTTTCCCGATACGGATACCGCCTATAAAGGCGCTGATAGCCGCGTATTATTGCGT
CATTGCTATGATTTGGCAAAGCAAAGGGGTTTTGAACTAGGTAATGTTGATGTCACTATTATTGCCCAAGCGCCCAAAAT
TGCACCCCATATTGAAGCTATGCGCCAAGTGCTTGCCACCGATTTAATGGCCGAATTGGATGATATTAACGTCAAAGCCA
CCACTACTGAAAAGTTAGGGTTTACAGGACGTAAAGAAGGCATTGCGGTAGAAGCAGTTGTGTTGATGAGCCGTAAACAA
GATTAA

Upstream 100 bases:

>100_bases
GTCACACATCCCGATGATCTGGAGCTGGCTGAACTCTTTTTAATGCGCGTTGCAAAATAGTTTATCTAGCATGTTACTAA
AGTAAGTTAGGAAATAATAA

Downstream 100 bases:

>100_bases
TTAACGAGATAGATAAGTCCCATGAGCGAACTGCATTACTTATATGGCAAACCACTCGGCACTGCCGATTTACGTACTCA
AAATAGCGATTTTATCGTGA

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MKIRIGHGFDVHKFGEVRPLILCGVEVPYETGLVAHSDGDVVLHAVSDAILGAMALGDIGKHFPDTDTAYKGADSRVLLR
HCYDLAKQRGFELGNVDVTIIAQAPKIAPHIEAMRQVLATDLMAELDDINVKATTTEKLGFTGRKEGIAVEAVVLMSRKQ
D

Sequences:

>Translated_161_residues
MKIRIGHGFDVHKFGEVRPLILCGVEVPYETGLVAHSDGDVVLHAVSDAILGAMALGDIGKHFPDTDTAYKGADSRVLLR
HCYDLAKQRGFELGNVDVTIIAQAPKIAPHIEAMRQVLATDLMAELDDINVKATTTEKLGFTGRKEGIAVEAVVLMSRKQ
D
>Mature_161_residues
MKIRIGHGFDVHKFGEVRPLILCGVEVPYETGLVAHSDGDVVLHAVSDAILGAMALGDIGKHFPDTDTAYKGADSRVLLR
HCYDLAKQRGFELGNVDVTIIAQAPKIAPHIEAMRQVLATDLMAELDDINVKATTTEKLGFTGRKEGIAVEAVVLMSRKQ
D

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=154, Percent_Identity=68.1818181818182, Blast_Score=203, Evalue=5e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_SHEPC (A4Y940)

Other databases:

- EMBL:   CP000681
- RefSeq:   YP_001184272.1
- ProteinModelPortal:   A4Y940
- SMR:   A4Y940
- STRING:   A4Y940
- GeneID:   5080821
- GenomeReviews:   CP000681_GR
- KEGG:   spc:Sputcn32_2754
- eggNOG:   COG0245
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- PhylomeDB:   A4Y940
- ProtClustDB:   PRK00084
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 17447; Mature: 17447

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRIGHGFDVHKFGEVRPLILCGVEVPYETGLVAHSDGDVVLHAVSDAILGAMALGDIG
CEEEECCCCCCCCCCCCCEEEEEECCCCHHCCEEEECCCCEEEEHHHHHHHHHHHHHHHH
KHFPDTDTAYKGADSRVLLRHCYDLAKQRGFELGNVDVTIIAQAPKIAPHIEAMRQVLAT
HCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEECCCCEEEEECCCCCCHHHHHHHHHHHH
DLMAELDDINVKATTTEKLGFTGRKEGIAVEAVVLMSRKQD
HHHHHHHCCCEEEECHHHCCCCCCCCCEEEEEEEEECCCCC
>Mature Secondary Structure
MKIRIGHGFDVHKFGEVRPLILCGVEVPYETGLVAHSDGDVVLHAVSDAILGAMALGDIG
CEEEECCCCCCCCCCCCCEEEEEECCCCHHCCEEEECCCCEEEEHHHHHHHHHHHHHHHH
KHFPDTDTAYKGADSRVLLRHCYDLAKQRGFELGNVDVTIIAQAPKIAPHIEAMRQVLAT
HCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEECCCCEEEEECCCCCCHHHHHHHHHHHH
DLMAELDDINVKATTTEKLGFTGRKEGIAVEAVVLMSRKQD
HHHHHHHCCCEEEECHHHCCCCCCCCCEEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA