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The map label for this gene is relA [H]

Identifier: 120598070

GI number: 120598070

Start: 1411878

End: 1414085

Strand: Direct

Name: relA [H]

Synonym: Sputw3181_1247

Alternate gene names: 120598070

Gene position: 1411878-1414085 (Clockwise)

Preceding gene: 120598069

Following gene: 120598072

Centisome position: 29.99

GC content: 49.09

Gene sequence:

>2208_bases
ATGGTCTCTGTTCGCGAAGCACACTTTAACGATCCCGATTTTAATCTAGACGATTGGGTGGCTCGTTATGTCAGTCAGGC
AGATGAGGCGCAGATGCTGCTCTCCTTGATAGGGCAGGTTGATGCCTTACCTGCCAAGAGTCCTGCGTCGAAGAGAGAAC
TGCTCGAACGTGCCCGAGAGATGATCGAAATCCTCGCACCGCTAAACATGGACATTGAAACCCTGCAGGCGGCGATTCTT
TTTGTGGTGTTCGATGCTGGGTTATTGACTGAAGAGGCTATCAAAGAAAAATTTGGTGAGCCGCTGGCGCGTTTAGTCGC
GAGTGTTGTGACCATGAACGCCATCGGCGCCTTGAAAATCAACCCTAATAGTCGTTCAACTGAGCCGCAAATCGACAATA
TTCGCCGCATGTTGCTGGCGATGGTCGAAGATGTGCGCGCGGTGGTGATCAAACTGGCCGAACGTGTGTGTTTGCTACGG
GCAGTGAAAAATGCCGATGAAGAAACTCGCGTGCTGCTGGCCCGTGAAATTGCCGATATTTACGCGCCGCTGGCTAACCG
TTTAGGGATTGGTCAGTTAAAGTGGGAATTAGAGGATATTTCCTTCCGCTATCTGCATCCAGATACCTATAAAGAAATCG
CTAAGCAGTTAGATGGCAAACGACTGGATCGTGAAGTTTTTATCGAAAAATTTGTTAGCCAATTACAACAACGACTCGAT
GAAGATCATATCCGCGCTAAGGTCTACGGCAGGCCGAAACACATCTACAGTATTTGGCGCAAGATGAAGGGTAAGCACCT
CAAATTTGATGAGTTATTTGATGTGCGCGCGGTGCGTATCGTGACTGAGCGTTTGCAGGATTGTTATGGTGCATTAGGTG
TGGTGCATACGCTTTGGCACCATATTCCGCGGGAATTTGACGATTATGTCGCTAACCCTAAACCTAACGGGTATCAATCG
ATCCATACCGTAGTGGTGGGGCCAGAGGGCAAAACCGTTGAAATTCAAATTCGTACCCAAGACATGCATGAAGATGCAGA
GCTGGGTGTGGCTGCGCATTGGAAATACAAAGAAGGCAATCATTCCGGTAAGCAAAGCGGCTACGAAGAAAAAATTAACT
GGCTGCGTAAAATTCTACAATGGCAGGAAGACGTGGTCGAAAGTGGCAACTTGGTTGAAGAAGTGCGCAGCCAAGTGTTT
GAAGATAGAGTCTATGTGTTCACCCCAAGCGGTGAAGTCGTCGACCTGCCACAGGGATCAACCGTGCTCGACTTTGCCTA
TTACATTCACTCCCAAGTAGGGCATAAATGTATTGGTGCTAAGGTCGATGGCCGTATTGTGCCGTTCACCTATCAGGTTG
AAACGGGTGAGCGGATCGAGATCATTACCTCGAAACAACCCAATCCCAAGCGCGACTGGCTTAACCCAAATTTAGGTTAT
ATCCGTACGGCGCGGGCGCGTTCGAAAATCCAGCATTGGTTCAAGCAGCAGGACAGAGACAAGAATATTGTCGCCGGCAA
AGAAATGCTCGAATCTGAACTGGCGCGGGTGAGCCTTAAAATTAAAGACGCCGCCATCGCGGTCGAGCGCTTTAATATGG
CGAGCATGGATGACTTACTGGCGGCGATTGGCGGCGGTGATGTGCGTCTGCATCAAGTGGTTAACCATATTCAGAGCCGT
TTAAGACTCGATGAAGTGTCAGAAGAAGATGCCGTCGAAGAATTAGTTAAGAAAGGCCAACATAAGCCGACCTCCAGTGG
CCGTGGTCAGGTTGAAGTCAACGGTGTAGGTAACTTGCTTAACCATATTGCCCGTTGTTGTCAGCCAGTTCCCGGTGATG
AGATCTTAGGTTTTATCACTAAGGGCCGTGGCATTTCTGTACATCGCGCCGATTGTGAACAAGTTAAAGAGTTGATGCGG
GTTCACCCTGAGCGCGGTGTCGATGTGGTCTGGGGCGAAAATTACTCCGGCGGTTATCGGATGCGTCTGCGGGTATTAGC
CCACGATCGCAGTGGCTTATTGCGTGATTTAACCTCAGTGCTGGCGGCTGAAAAATCCAATGTATTGGCCATGAGCTCCT
CATCGGATACTAAAAACCAGACTGCCGCAATTGAGTTGGAACTGGAGCTTTATAATCTCGATGGTTTATCGCGAGTGTTA
TCTAAGTTATCTCAGGTCGATAGCGTGATCGAGGCAAGACGACTTTAG

Upstream 100 bases:

>100_bases
TTTGGTCAGTTAGGTTTGACAAGTTCGCGCCAATGCTGAAGATAGGAGAGCCGAAGTGGTGCCCACTTTCTCTCTGAGCA
CGGATAAGGAAACAAATTAG

Downstream 100 bases:

>100_bases
GTTTAGCAATGCCTTTCGAAGCGAATAGCCTTGAAATCACTAGCAATATAGAAGATATAGAGAAGCTATCTAGAAGCAGA
GATAAAGGATTGGGGCAGCA

Product: (p)ppGpp synthetase I, SpoT/RelA

Products: NA

Alternate protein names: (p)ppGpp synthase; ATP:GTP 3'-pyrophosphotransferase; ppGpp synthase I [H]

Number of amino acids: Translated: 735; Mature: 735

Protein sequence:

>735_residues
MVSVREAHFNDPDFNLDDWVARYVSQADEAQMLLSLIGQVDALPAKSPASKRELLERAREMIEILAPLNMDIETLQAAIL
FVVFDAGLLTEEAIKEKFGEPLARLVASVVTMNAIGALKINPNSRSTEPQIDNIRRMLLAMVEDVRAVVIKLAERVCLLR
AVKNADEETRVLLAREIADIYAPLANRLGIGQLKWELEDISFRYLHPDTYKEIAKQLDGKRLDREVFIEKFVSQLQQRLD
EDHIRAKVYGRPKHIYSIWRKMKGKHLKFDELFDVRAVRIVTERLQDCYGALGVVHTLWHHIPREFDDYVANPKPNGYQS
IHTVVVGPEGKTVEIQIRTQDMHEDAELGVAAHWKYKEGNHSGKQSGYEEKINWLRKILQWQEDVVESGNLVEEVRSQVF
EDRVYVFTPSGEVVDLPQGSTVLDFAYYIHSQVGHKCIGAKVDGRIVPFTYQVETGERIEIITSKQPNPKRDWLNPNLGY
IRTARARSKIQHWFKQQDRDKNIVAGKEMLESELARVSLKIKDAAIAVERFNMASMDDLLAAIGGGDVRLHQVVNHIQSR
LRLDEVSEEDAVEELVKKGQHKPTSSGRGQVEVNGVGNLLNHIARCCQPVPGDEILGFITKGRGISVHRADCEQVKELMR
VHPERGVDVVWGENYSGGYRMRLRVLAHDRSGLLRDLTSVLAAEKSNVLAMSSSSDTKNQTAAIELELELYNLDGLSRVL
SKLSQVDSVIEARRL

Sequences:

>Translated_735_residues
MVSVREAHFNDPDFNLDDWVARYVSQADEAQMLLSLIGQVDALPAKSPASKRELLERAREMIEILAPLNMDIETLQAAIL
FVVFDAGLLTEEAIKEKFGEPLARLVASVVTMNAIGALKINPNSRSTEPQIDNIRRMLLAMVEDVRAVVIKLAERVCLLR
AVKNADEETRVLLAREIADIYAPLANRLGIGQLKWELEDISFRYLHPDTYKEIAKQLDGKRLDREVFIEKFVSQLQQRLD
EDHIRAKVYGRPKHIYSIWRKMKGKHLKFDELFDVRAVRIVTERLQDCYGALGVVHTLWHHIPREFDDYVANPKPNGYQS
IHTVVVGPEGKTVEIQIRTQDMHEDAELGVAAHWKYKEGNHSGKQSGYEEKINWLRKILQWQEDVVESGNLVEEVRSQVF
EDRVYVFTPSGEVVDLPQGSTVLDFAYYIHSQVGHKCIGAKVDGRIVPFTYQVETGERIEIITSKQPNPKRDWLNPNLGY
IRTARARSKIQHWFKQQDRDKNIVAGKEMLESELARVSLKIKDAAIAVERFNMASMDDLLAAIGGGDVRLHQVVNHIQSR
LRLDEVSEEDAVEELVKKGQHKPTSSGRGQVEVNGVGNLLNHIARCCQPVPGDEILGFITKGRGISVHRADCEQVKELMR
VHPERGVDVVWGENYSGGYRMRLRVLAHDRSGLLRDLTSVLAAEKSNVLAMSSSSDTKNQTAAIELELELYNLDGLSRVL
SKLSQVDSVIEARRL
>Mature_735_residues
MVSVREAHFNDPDFNLDDWVARYVSQADEAQMLLSLIGQVDALPAKSPASKRELLERAREMIEILAPLNMDIETLQAAIL
FVVFDAGLLTEEAIKEKFGEPLARLVASVVTMNAIGALKINPNSRSTEPQIDNIRRMLLAMVEDVRAVVIKLAERVCLLR
AVKNADEETRVLLAREIADIYAPLANRLGIGQLKWELEDISFRYLHPDTYKEIAKQLDGKRLDREVFIEKFVSQLQQRLD
EDHIRAKVYGRPKHIYSIWRKMKGKHLKFDELFDVRAVRIVTERLQDCYGALGVVHTLWHHIPREFDDYVANPKPNGYQS
IHTVVVGPEGKTVEIQIRTQDMHEDAELGVAAHWKYKEGNHSGKQSGYEEKINWLRKILQWQEDVVESGNLVEEVRSQVF
EDRVYVFTPSGEVVDLPQGSTVLDFAYYIHSQVGHKCIGAKVDGRIVPFTYQVETGERIEIITSKQPNPKRDWLNPNLGY
IRTARARSKIQHWFKQQDRDKNIVAGKEMLESELARVSLKIKDAAIAVERFNMASMDDLLAAIGGGDVRLHQVVNHIQSR
LRLDEVSEEDAVEELVKKGQHKPTSSGRGQVEVNGVGNLLNHIARCCQPVPGDEILGFITKGRGISVHRADCEQVKELMR
VHPERGVDVVWGENYSGGYRMRLRVLAHDRSGLLRDLTSVLAAEKSNVLAMSSSSDTKNQTAAIELELELYNLDGLSRVL
SKLSQVDSVIEARRL

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which i

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Escherichia coli, GI1789147, Length=741, Percent_Identity=59.2442645074224, Blast_Score=892, Evalue=0.0,
Organism=Escherichia coli, GI1790082, Length=675, Percent_Identity=33.1851851851852, Blast_Score=410, Evalue=1e-115,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =2.7.6.5 [H]

Molecular weight: Translated: 83294; Mature: 83294

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSVREAHFNDPDFNLDDWVARYVSQADEAQMLLSLIGQVDALPAKSPASKRELLERARE
CCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
MIEILAPLNMDIETLQAAILFVVFDAGLLTEEAIKEKFGEPLARLVASVVTMNAIGALKI
HHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEE
NPNSRSTEPQIDNIRRMLLAMVEDVRAVVIKLAERVCLLRAVKNADEETRVLLAREIADI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
YAPLANRLGIGQLKWELEDISFRYLHPDTYKEIAKQLDGKRLDREVFIEKFVSQLQQRLD
HHHHHHHCCCCCEEEEEECCEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
EDHIRAKVYGRPKHIYSIWRKMKGKHLKFDELFDVRAVRIVTERLQDCYGALGVVHTLWH
HHHHEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HIPREFDDYVANPKPNGYQSIHTVVVGPEGKTVEIQIRTQDMHEDAELGVAAHWKYKEGN
HHCHHHHHHCCCCCCCCCCEEEEEEECCCCCEEEEEEEECCCCCCHHCCEEEEEEECCCC
HSGKQSGYEEKINWLRKILQWQEDVVESGNLVEEVRSQVFEDRVYVFTPSGEVVDLPQGS
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEECCCCCEEECCCCC
TVLDFAYYIHSQVGHKCIGAKVDGRIVPFTYQVETGERIEIITSKQPNPKRDWLNPNLGY
HHHHHHHHHHHHHCCHHCCCCCCCEEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCC
IRTARARSKIQHWFKQQDRDKNIVAGKEMLESELARVSLKIKDAAIAVERFNMASMDDLL
EEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEEEHHHHHHHHHCCCCCHHHHH
AAIGGGDVRLHQVVNHIQSRLRLDEVSEEDAVEELVKKGQHKPTSSGRGQVEVNGVGNLL
HHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCEEEECCHHHHH
NHIARCCQPVPGDEILGFITKGRGISVHRADCEQVKELMRVHPERGVDVVWGENYSGGYR
HHHHHHCCCCCCHHHHHHHCCCCCCEEEHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEE
MRLRVLAHDRSGLLRDLTSVLAAEKSNVLAMSSSSDTKNQTAAIELELELYNLDGLSRVL
EEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEEEEECCCHHHHHH
SKLSQVDSVIEARRL
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MVSVREAHFNDPDFNLDDWVARYVSQADEAQMLLSLIGQVDALPAKSPASKRELLERARE
CCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
MIEILAPLNMDIETLQAAILFVVFDAGLLTEEAIKEKFGEPLARLVASVVTMNAIGALKI
HHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEE
NPNSRSTEPQIDNIRRMLLAMVEDVRAVVIKLAERVCLLRAVKNADEETRVLLAREIADI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
YAPLANRLGIGQLKWELEDISFRYLHPDTYKEIAKQLDGKRLDREVFIEKFVSQLQQRLD
HHHHHHHCCCCCEEEEEECCEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
EDHIRAKVYGRPKHIYSIWRKMKGKHLKFDELFDVRAVRIVTERLQDCYGALGVVHTLWH
HHHHEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HIPREFDDYVANPKPNGYQSIHTVVVGPEGKTVEIQIRTQDMHEDAELGVAAHWKYKEGN
HHCHHHHHHCCCCCCCCCCEEEEEEECCCCCEEEEEEEECCCCCCHHCCEEEEEEECCCC
HSGKQSGYEEKINWLRKILQWQEDVVESGNLVEEVRSQVFEDRVYVFTPSGEVVDLPQGS
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEECCCCCEEECCCCC
TVLDFAYYIHSQVGHKCIGAKVDGRIVPFTYQVETGERIEIITSKQPNPKRDWLNPNLGY
HHHHHHHHHHHHHCCHHCCCCCCCEEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCC
IRTARARSKIQHWFKQQDRDKNIVAGKEMLESELARVSLKIKDAAIAVERFNMASMDDLL
EEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEEEHHHHHHHHHCCCCCHHHHH
AAIGGGDVRLHQVVNHIQSRLRLDEVSEEDAVEELVKKGQHKPTSSGRGQVEVNGVGNLL
HHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCEEEECCHHHHH
NHIARCCQPVPGDEILGFITKGRGISVHRADCEQVKELMRVHPERGVDVVWGENYSGGYR
HHHHHHCCCCCCHHHHHHHCCCCCCEEEHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEE
MRLRVLAHDRSGLLRDLTSVLAAEKSNVLAMSSSSDTKNQTAAIELELELYNLDGLSRVL
EEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEEEEECCCHHHHHH
SKLSQVDSVIEARRL
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]