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The map label for this gene is rumA

Identifier: 120598069

GI number: 120598069

Start: 1410373

End: 1411725

Strand: Direct

Name: rumA

Synonym: Sputw3181_1246

Alternate gene names: 120598069

Gene position: 1410373-1411725 (Clockwise)

Preceding gene: 120598066

Following gene: 120598070

Centisome position: 29.95

GC content: 47.52

Gene sequence:

>1353_bases
ATGGCACAATTTTTTAAAGCAAAACCAAATAGTTCCAAACAGTTGTCAGCTAAGTTGTCGCTAAATGTGGATCAGCTCGA
TCACTTAGGCGCTGGCATTGCTCAGTATCAAGGTAAAGTCGTGTTCATTCCCGGCGCTTTGCCCGATGAAACCGTGACAG
TACAGCTCACCGAACAAAAGAAAAATTATGCCCGTGCCAAGTTGATTAAAGTCGATGCCCAGAGCCCTGAGCGCGTCGAG
CCTGAGTGTCCACACTATCATACTTGTGGCGGCTGCGACTTACAGCATATGTCATTATCGGGTCAACGTGAGCATAAAGA
AGCCGCGTTGCTGGATATTATGGCAAAATTTGCAGGTACTGAAGGCGGTGCTTTATCACCCGCATTAACCGGTGAAGGCT
GGCATTATCGCCGCCGCGCCCGTCTTGCAACCTTATTTGATAAAAATACCAAGCACTTAAGTTTAGGCTTTCGCGCGGCG
AGCAGCAGTAATGTGGTGCCTATTAGCCAATGCCAAGTATTAGCTAAGCCGTTATCGGACTTGATCGTGCCATTTGCTAA
GTTGCTAAATCAATTGTCGGCTAAAGCCAGCTTAGGCCACCTTGAGTTGATTGCCGCCGATAATGGTCACTTCGCCGTGC
TGCGGATCACTAAGGCGTTAAACGATAAGGATCTCGCTAAGCTGTCAGCCTTTGCCGAGCAGCATCAGATCTATATTTGT
CTGCAGGATAACGAAGGCCAATTCCAAGGCGTCGGCGTCGAGTTAGTGTTACCTGTTTATCAACTGTTAGATGAAAATGC
GCAGTCGGATGCGGTGAGTTTAAGTTTCACCCCCGGTAACTTTGTGCAGGTCAATAGCCAAATTAACAAGGCCATGGTCG
CTCAAGCGATGGATTGGTTAGCGCCAGCCCCTGATGAGCGCATTTTGGATTTGTTCTGTGGTATGGGTAATTTCAGTTTG
CCCTTAGCGAAAATGGGCGCGGATGTGATTGGCGTCGAAGGGGTTGCCGAGATGGTAAGCCAAGCGCGGGTGAACGCTAA
AGCTAACAATCTGGATAAACTCACCTTTTATCATGGCGATTTAAGTGCAGATTTATCCCTTGAGCCTTGGATGGGAAAAA
TTGATAAATTACTGCTCGATCCTGCTCGTGCGGGGGCATTTGAAAGCTTGCAATGGCTAAAGAAAATGAAGCCGCGTAAA
GTTCTGTATGTGTCTTGTAATCCTGCCAGCTTAGCCCGTGACAGTGCTGTACTATTAGAGCGCGGTTACCGATTACAGCG
ATTGGGACTTATCGACATGTTCCCACAAACACACCATATTGAAGCCATGGCACTGTTTGAATTAACCAAGTAA

Upstream 100 bases:

>100_bases
AGCAGCAGCGGTGTTTCCTGAAACAGATACGATAATAGTGTTATAAGACTGCCAGAAAAAGGCCCGTCTTTGCCAGTTTT
TTATTGATTTAGAGGCCGAA

Downstream 100 bases:

>100_bases
TTTGCGCTGACAAAGTAAGCAAAGCGTTTGAATAACGTAAAAAATGTGAATATTTGGTCAGTTAGGTTTGACAAGTTCGC
GCCAATGCTGAAGATAGGAG

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase [H]

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MAQFFKAKPNSSKQLSAKLSLNVDQLDHLGAGIAQYQGKVVFIPGALPDETVTVQLTEQKKNYARAKLIKVDAQSPERVE
PECPHYHTCGGCDLQHMSLSGQREHKEAALLDIMAKFAGTEGGALSPALTGEGWHYRRRARLATLFDKNTKHLSLGFRAA
SSSNVVPISQCQVLAKPLSDLIVPFAKLLNQLSAKASLGHLELIAADNGHFAVLRITKALNDKDLAKLSAFAEQHQIYIC
LQDNEGQFQGVGVELVLPVYQLLDENAQSDAVSLSFTPGNFVQVNSQINKAMVAQAMDWLAPAPDERILDLFCGMGNFSL
PLAKMGADVIGVEGVAEMVSQARVNAKANNLDKLTFYHGDLSADLSLEPWMGKIDKLLLDPARAGAFESLQWLKKMKPRK
VLYVSCNPASLARDSAVLLERGYRLQRLGLIDMFPQTHHIEAMALFELTK

Sequences:

>Translated_450_residues
MAQFFKAKPNSSKQLSAKLSLNVDQLDHLGAGIAQYQGKVVFIPGALPDETVTVQLTEQKKNYARAKLIKVDAQSPERVE
PECPHYHTCGGCDLQHMSLSGQREHKEAALLDIMAKFAGTEGGALSPALTGEGWHYRRRARLATLFDKNTKHLSLGFRAA
SSSNVVPISQCQVLAKPLSDLIVPFAKLLNQLSAKASLGHLELIAADNGHFAVLRITKALNDKDLAKLSAFAEQHQIYIC
LQDNEGQFQGVGVELVLPVYQLLDENAQSDAVSLSFTPGNFVQVNSQINKAMVAQAMDWLAPAPDERILDLFCGMGNFSL
PLAKMGADVIGVEGVAEMVSQARVNAKANNLDKLTFYHGDLSADLSLEPWMGKIDKLLLDPARAGAFESLQWLKKMKPRK
VLYVSCNPASLARDSAVLLERGYRLQRLGLIDMFPQTHHIEAMALFELTK
>Mature_449_residues
AQFFKAKPNSSKQLSAKLSLNVDQLDHLGAGIAQYQGKVVFIPGALPDETVTVQLTEQKKNYARAKLIKVDAQSPERVEP
ECPHYHTCGGCDLQHMSLSGQREHKEAALLDIMAKFAGTEGGALSPALTGEGWHYRRRARLATLFDKNTKHLSLGFRAAS
SSNVVPISQCQVLAKPLSDLIVPFAKLLNQLSAKASLGHLELIAADNGHFAVLRITKALNDKDLAKLSAFAEQHQIYICL
QDNEGQFQGVGVELVLPVYQLLDENAQSDAVSLSFTPGNFVQVNSQINKAMVAQAMDWLAPAPDERILDLFCGMGNFSLP
LAKMGADVIGVEGVAEMVSQARVNAKANNLDKLTFYHGDLSADLSLEPWMGKIDKLLLDPARAGAFESLQWLKKMKPRKV
LYVSCNPASLARDSAVLLERGYRLQRLGLIDMFPQTHHIEAMALFELTK

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=207, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI51173878, Length=207, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1789148, Length=452, Percent_Identity=42.9203539823009, Blast_Score=378, Evalue=1e-106,
Organism=Escherichia coli, GI1787083, Length=379, Percent_Identity=24.802110817942, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI1790403, Length=183, Percent_Identity=28.9617486338798, Blast_Score=82, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24666579, Length=196, Percent_Identity=32.1428571428571, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.190 [H]

Molecular weight: Translated: 49313; Mature: 49182

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS50926 TRAM ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQFFKAKPNSSKQLSAKLSLNVDQLDHLGAGIAQYQGKVVFIPGALPDETVTVQLTEQK
CCCCCCCCCCCCCEEEEEEECCHHHHHHHCCCHHHHCCEEEEECCCCCCCEEEEEEECCH
KNYARAKLIKVDAQSPERVEPECPHYHTCGGCDLQHMSLSGQREHKEAALLDIMAKFAGT
HCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCC
EGGALSPALTGEGWHYRRRARLATLFDKNTKHLSLGFRAASSSNVVPISQCQVLAKPLSD
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCEEHHHHHHHHHHHHH
LIVPFAKLLNQLSAKASLGHLELIAADNGHFAVLRITKALNDKDLAKLSAFAEQHQIYIC
HHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEEECCCCHHHHHHHHHHCCCEEEEE
LQDNEGQFQGVGVELVLPVYQLLDENAQSDAVSLSFTPGNFVQVNSQINKAMVAQAMDWL
EECCCCCEEECCHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEECCHHHHHHHHHHHHHH
APAPDERILDLFCGMGNFSLPLAKMGADVIGVEGVAEMVSQARVNAKANNLDKLTFYHGD
CCCCCHHHHHHHHCCCCCCCCHHHHCCCEECHHHHHHHHHHHHCCCCCCCCCEEEEEECC
LSADLSLEPWMGKIDKLLLDPARAGAFESLQWLKKMKPRKVLYVSCNPASLARDSAVLLE
CCCCCCCCCHHHHHHHHHHCCHHCCHHHHHHHHHHCCCCEEEEEECCCHHHCCCHHHHHH
RGYRLQRLGLIDMFPQTHHIEAMALFELTK
CCCCEEECCHHHCCCCCCCEEEEEEEEECC
>Mature Secondary Structure 
AQFFKAKPNSSKQLSAKLSLNVDQLDHLGAGIAQYQGKVVFIPGALPDETVTVQLTEQK
CCCCCCCCCCCCEEEEEEECCHHHHHHHCCCHHHHCCEEEEECCCCCCCEEEEEEECCH
KNYARAKLIKVDAQSPERVEPECPHYHTCGGCDLQHMSLSGQREHKEAALLDIMAKFAGT
HCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCC
EGGALSPALTGEGWHYRRRARLATLFDKNTKHLSLGFRAASSSNVVPISQCQVLAKPLSD
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCEEHHHHHHHHHHHHH
LIVPFAKLLNQLSAKASLGHLELIAADNGHFAVLRITKALNDKDLAKLSAFAEQHQIYIC
HHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEEECCCCHHHHHHHHHHCCCEEEEE
LQDNEGQFQGVGVELVLPVYQLLDENAQSDAVSLSFTPGNFVQVNSQINKAMVAQAMDWL
EECCCCCEEECCHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEECCHHHHHHHHHHHHHH
APAPDERILDLFCGMGNFSLPLAKMGADVIGVEGVAEMVSQARVNAKANNLDKLTFYHGD
CCCCCHHHHHHHHCCCCCCCCHHHHCCCEECHHHHHHHHHHHHCCCCCCCCCEEEEEECC
LSADLSLEPWMGKIDKLLLDPARAGAFESLQWLKKMKPRKVLYVSCNPASLARDSAVLLE
CCCCCCCCCHHHHHHHHHHCCHHCCHHHHHHHHHHCCCCEEEEEECCCHHHCCCHHHHHH
RGYRLQRLGLIDMFPQTHHIEAMALFELTK
CCCCEEECCHHHCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA