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The map label for this gene is caiD [C]

Identifier: 120597468

GI number: 120597468

Start: 693639

End: 694445

Strand: Direct

Name: caiD [C]

Synonym: Sputw3181_0637

Alternate gene names: 120597468

Gene position: 693639-694445 (Clockwise)

Preceding gene: 120597465

Following gene: 120597471

Centisome position: 14.73

GC content: 45.97

Gene sequence:

>807_bases
ATGAGTACCCAATGTGTAAAAGTCACTATCAGCGATGGCATTGCCTATGTGCAGCTTAATCGTCCTGAAAAAATGAACGC
GATTAACTATTTAATGTTTAGCGAATTAGATAGAACAATTAAAAAAATTCAGAAGAATCGAGCCATTCGAGTGGTTATTC
TTTCAGGCGCTGGTGGAAACTTTAGTTCAGGGCTTGATGTTAAAAGTGTTATGTCAGCACCGATGCAAGCGGTGAAATTG
CTCTTTAAATGGTTACCCGGAAACGCCAATCTCGCCCAACGCGTTTCCCTTGGCTGGCAGCGATTACCTATCCCTGTTAT
TGCCGTGCTTGATGGATGTTGCTTTGGTGGCGGTATGCAGATTGCGCTCGGCGCAGATATCCGCATCGCTAGCCATGACA
GCCAGTTATCGGTTATGGAAGCTAAATGGGGGCTAGTACCCGATATGGCGGGATTGGTTTCACTTAGGCAAATTATGCCT
AAAGATCAAGCGATGTTATTAAGTTTGACTGCTAAGGTTCTTTCAGGGGAACAAGCCTTCGCTCTTGGACTAGTCACTCA
GTTATCTGAGCAGCCAATGGCTGCTGCAGAGCAATTAGCCAGAGAGCTAATCAAGACATCACCCGATGCTACCGCGGCGA
TTAAGCTCAGCATTAATAGGAGTTGGACGGCCTCAGTACGTAGCCTCTTAAGCCGTGAGTCATTGAGCCAAATTCGGCTC
TTGTTAGGCAAAAATCGTACTATTGCAGCGGTACGGCAGCTTAAAGATCCTGAAAAACCTTATCAGGATCGCCAGTCTGG
TTGGTAA

Upstream 100 bases:

>100_bases
TTTTGACTAAGGTCAAAATTAAGGTTTATGTTATATCTTTTAGTGGATTAGACCAAATGTGTTAACATTTGTTCTGGACT
GACCAGTAACGGAATATTTG

Downstream 100 bases:

>100_bases
TACTGGGAATGTTGAACGCACTTAGGAATAGATGAGATATTTCGTAAAAACCTATCTCTCAGTGTGCGCCGCTCATTCTG
GCGGCGAACCCCTTGATCGT

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MSTQCVKVTISDGIAYVQLNRPEKMNAINYLMFSELDRTIKKIQKNRAIRVVILSGAGGNFSSGLDVKSVMSAPMQAVKL
LFKWLPGNANLAQRVSLGWQRLPIPVIAVLDGCCFGGGMQIALGADIRIASHDSQLSVMEAKWGLVPDMAGLVSLRQIMP
KDQAMLLSLTAKVLSGEQAFALGLVTQLSEQPMAAAEQLARELIKTSPDATAAIKLSINRSWTASVRSLLSRESLSQIRL
LLGKNRTIAAVRQLKDPEKPYQDRQSGW

Sequences:

>Translated_268_residues
MSTQCVKVTISDGIAYVQLNRPEKMNAINYLMFSELDRTIKKIQKNRAIRVVILSGAGGNFSSGLDVKSVMSAPMQAVKL
LFKWLPGNANLAQRVSLGWQRLPIPVIAVLDGCCFGGGMQIALGADIRIASHDSQLSVMEAKWGLVPDMAGLVSLRQIMP
KDQAMLLSLTAKVLSGEQAFALGLVTQLSEQPMAAAEQLARELIKTSPDATAAIKLSINRSWTASVRSLLSRESLSQIRL
LLGKNRTIAAVRQLKDPEKPYQDRQSGW
>Mature_267_residues
STQCVKVTISDGIAYVQLNRPEKMNAINYLMFSELDRTIKKIQKNRAIRVVILSGAGGNFSSGLDVKSVMSAPMQAVKLL
FKWLPGNANLAQRVSLGWQRLPIPVIAVLDGCCFGGGMQIALGADIRIASHDSQLSVMEAKWGLVPDMAGLVSLRQIMPK
DQAMLLSLTAKVLSGEQAFALGLVTQLSEQPMAAAEQLARELIKTSPDATAAIKLSINRSWTASVRSLLSRESLSQIRLL
LGKNRTIAAVRQLKDPEKPYQDRQSGW

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI70995211, Length=227, Percent_Identity=31.2775330396476, Blast_Score=95, Evalue=8e-20,
Organism=Homo sapiens, GI194097323, Length=217, Percent_Identity=28.110599078341, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI157694516, Length=239, Percent_Identity=25.1046025104602, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI213417737, Length=239, Percent_Identity=25.1046025104602, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI45643119, Length=181, Percent_Identity=30.3867403314917, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI260275230, Length=181, Percent_Identity=30.3867403314917, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI260274832, Length=181, Percent_Identity=30.3867403314917, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI68989263, Length=195, Percent_Identity=25.6410256410256, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI20127408, Length=182, Percent_Identity=28.021978021978, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI31542718, Length=196, Percent_Identity=26.530612244898, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI4502327, Length=241, Percent_Identity=26.5560165975104, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI221142681, Length=217, Percent_Identity=31.7972350230415, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1787660, Length=227, Percent_Identity=31.7180616740088, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1788682, Length=198, Percent_Identity=30.8080808080808, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1790281, Length=232, Percent_Identity=25.4310344827586, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1787659, Length=249, Percent_Identity=26.1044176706827, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1788597, Length=218, Percent_Identity=26.605504587156, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI87082183, Length=219, Percent_Identity=22.8310502283105, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17540714, Length=225, Percent_Identity=32.4444444444444, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17536985, Length=262, Percent_Identity=23.6641221374046, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI25145438, Length=225, Percent_Identity=27.5555555555556, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17560910, Length=211, Percent_Identity=26.0663507109005, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17558304, Length=282, Percent_Identity=24.468085106383, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17554946, Length=232, Percent_Identity=22.8448275862069, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI25144276, Length=221, Percent_Identity=28.0542986425339, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17508951, Length=221, Percent_Identity=28.0542986425339, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17508953, Length=221, Percent_Identity=28.0542986425339, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=191, Percent_Identity=27.7486910994764, Blast_Score=76, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24653139, Length=254, Percent_Identity=29.5275590551181, Blast_Score=104, Evalue=5e-23,
Organism=Drosophila melanogaster, GI28571729, Length=191, Percent_Identity=29.8429319371728, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI28571730, Length=191, Percent_Identity=29.8429319371728, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI20129971, Length=225, Percent_Identity=24, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24653477, Length=225, Percent_Identity=24, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19920382, Length=257, Percent_Identity=24.124513618677, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI19922422, Length=211, Percent_Identity=27.0142180094787, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21357171, Length=177, Percent_Identity=25.9887005649718, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19921000, Length=243, Percent_Identity=26.3374485596708, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24583077, Length=243, Percent_Identity=26.3374485596708, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24583079, Length=243, Percent_Identity=26.3374485596708, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI19921018, Length=223, Percent_Identity=24.2152466367713, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 29217; Mature: 29085

Theoretical pI: Translated: 10.54; Mature: 10.54

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTQCVKVTISDGIAYVQLNRPEKMNAINYLMFSELDRTIKKIQKNRAIRVVILSGAGGN
CCCEEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCC
FSSGLDVKSVMSAPMQAVKLLFKWLPGNANLAQRVSLGWQRLPIPVIAVLDGCCFGGGMQ
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCHHHHHHHHCCCCCCCEE
IALGADIRIASHDSQLSVMEAKWGLVPDMAGLVSLRQIMPKDQAMLLSLTAKVLSGEQAF
EEECCCEEEECCCCCEEEEHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHH
ALGLVTQLSEQPMAAAEQLARELIKTSPDATAAIKLSINRSWTASVRSLLSRESLSQIRL
HHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHH
LLGKNRTIAAVRQLKDPEKPYQDRQSGW
HHCCCCHHHHHHHHCCCCCCCCHHCCCC
>Mature Secondary Structure 
STQCVKVTISDGIAYVQLNRPEKMNAINYLMFSELDRTIKKIQKNRAIRVVILSGAGGN
CCEEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCC
FSSGLDVKSVMSAPMQAVKLLFKWLPGNANLAQRVSLGWQRLPIPVIAVLDGCCFGGGMQ
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCHHHHHHHHCCCCCCCEE
IALGADIRIASHDSQLSVMEAKWGLVPDMAGLVSLRQIMPKDQAMLLSLTAKVLSGEQAF
EEECCCEEEECCCCCEEEEHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHH
ALGLVTQLSEQPMAAAEQLARELIKTSPDATAAIKLSINRSWTASVRSLLSRESLSQIRL
HHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHH
LLGKNRTIAAVRQLKDPEKPYQDRQSGW
HHCCCCHHHHHHHHCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA