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The map label for this gene is ampE [H]

Identifier: 120597355

GI number: 120597355

Start: 562626

End: 563477

Strand: Direct

Name: ampE [H]

Synonym: Sputw3181_0524

Alternate gene names: 120597355

Gene position: 562626-563477 (Clockwise)

Preceding gene: 120597354

Following gene: 120597356

Centisome position: 11.95

GC content: 42.84

Gene sequence:

>852_bases
ATGGCATTATTTTCATTGTTAGTCGCCATTTTGGTGGAGCGACTTAAATTTCTTCCCTCGTCGTGGCAATTTGATCGATT
GCTACAGTCTTATCAGATGACGTTTTTTGGTGATAAAGCGTCTTTGACCACACCTATGATGGCACTCGCTCTAGTGTTGC
CTGCTTTGGTGGTGTATGTCTTATCTTGGTTAGTTGCAGGAATGTTTTGGGGAATACTCAGTTTGGCGCTTTGGGTTTTT
GTGGCGGCAATTTGTTTTAATCACCTGAAACAACGTGAAATTTTTAAAAAATATATGCAAGCCGCTTGTCGTTCGGATGT
GCAAGCCTGCTATCTTTATGCCGCAGAGCTTGATTGTAGCGAATGCCTAGATGCGGTATCTGAAAAGGATTTAGGGGCTA
AGGTTGGCCAGAGTGTGGCATGGATTAACTATCGTTATTATGGCGCTGTAGCACTGTTTTTGATTTTCTTTGGGCCCGTC
GGCACAGTGCTCTATTGCACAGTACGGTATTATGCTGAAGAAAATGCGCGAAAATCCCTTAATTTACCTTTAGTGGATGA
GTTTATCGCCGTTTTAGATTGGATTCCGAGCCGGCTATTTGCCTTTGGCTATGCATTAAGCGGGCAATTTAGTGAGGGAT
TAACGGCATGGCGTAATAACGCGCTACGTTTTAATACCAGTGCAAGAAGGGTGATTACTGAAACGGCAGTGGCTGCGCAA
CCTTTACCTGAGGTTTCTGATGCACCTATTTGTGTACAATCGACCCTAGCGTTATTAGTGTTGAGTAAACGTAACTTTAC
ACTTATGGTTGCAATTGTTTCATTATTGACCATTTTCGGATTGGTGAGTTAA

Upstream 100 bases:

>100_bases
GGGATGACGGCTTAAGCGATTCTTCTAGAATCTAGAATCTAGGGTCTAGAATCTAGCAGGGCACCAGCCCGTCCTGTTTT
TTATACAAGGAGTACGACCG

Downstream 100 bases:

>100_bases
AAGAAGGACGTTGCGCTGCGAGAATCGAGAATCTAGGACGCTTCGCTGCTAGGGCGTCGCTGTGCTCCTTCTAGATCCTA
GAGAGCGTAAGTACACAAAG

Product: regulatory protein AmpE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MALFSLLVAILVERLKFLPSSWQFDRLLQSYQMTFFGDKASLTTPMMALALVLPALVVYVLSWLVAGMFWGILSLALWVF
VAAICFNHLKQREIFKKYMQAACRSDVQACYLYAAELDCSECLDAVSEKDLGAKVGQSVAWINYRYYGAVALFLIFFGPV
GTVLYCTVRYYAEENARKSLNLPLVDEFIAVLDWIPSRLFAFGYALSGQFSEGLTAWRNNALRFNTSARRVITETAVAAQ
PLPEVSDAPICVQSTLALLVLSKRNFTLMVAIVSLLTIFGLVS

Sequences:

>Translated_283_residues
MALFSLLVAILVERLKFLPSSWQFDRLLQSYQMTFFGDKASLTTPMMALALVLPALVVYVLSWLVAGMFWGILSLALWVF
VAAICFNHLKQREIFKKYMQAACRSDVQACYLYAAELDCSECLDAVSEKDLGAKVGQSVAWINYRYYGAVALFLIFFGPV
GTVLYCTVRYYAEENARKSLNLPLVDEFIAVLDWIPSRLFAFGYALSGQFSEGLTAWRNNALRFNTSARRVITETAVAAQ
PLPEVSDAPICVQSTLALLVLSKRNFTLMVAIVSLLTIFGLVS
>Mature_282_residues
ALFSLLVAILVERLKFLPSSWQFDRLLQSYQMTFFGDKASLTTPMMALALVLPALVVYVLSWLVAGMFWGILSLALWVFV
AAICFNHLKQREIFKKYMQAACRSDVQACYLYAAELDCSECLDAVSEKDLGAKVGQSVAWINYRYYGAVALFLIFFGPVG
TVLYCTVRYYAEENARKSLNLPLVDEFIAVLDWIPSRLFAFGYALSGQFSEGLTAWRNNALRFNTSARRVITETAVAAQP
LPEVSDAPICVQSTLALLVLSKRNFTLMVAIVSLLTIFGLVS

Specific function: Putative signaling protein in beta-lactamase regulation. AmpE seems not to act as a direct sensor for beta-lactams [H]

COG id: COG3725

COG function: function code V; Membrane protein required for beta-lactamase induction

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786301, Length=297, Percent_Identity=27.2727272727273, Blast_Score=92, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: NA

Molecular weight: Translated: 31606; Mature: 31474

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: PS01171 RIBOSOMAL_L21E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ALFSLLVAILVERLKFLPSSWQFDRLLQSYQMTFFGDKASLTTPMMALALVLPALVVYV
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LSWLVAGMFWGILSLALWVFVAAICFNHLKQREIFKKYMQAACRSDVQACYLYAAELDCS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
ECLDAVSEKDLGAKVGQSVAWINYRYYGAVALFLIFFGPVGTVLYCTVRYYAEENARKSL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NLPLVDEFIAVLDWIPSRLFAFGYALSGQFSEGLTAWRNNALRFNTSARRVITETAVAAQ
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEECCHHHHHHHHHHHHHC
PLPEVSDAPICVQSTLALLVLSKRNFTLMVAIVSLLTIFGLVS
CCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2691840; 2607970; 9278503 [H]