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The map label for this gene is ampD [H]

Identifier: 120597354

GI number: 120597354

Start: 561884

End: 562426

Strand: Direct

Name: ampD [H]

Synonym: Sputw3181_0523

Alternate gene names: 120597354

Gene position: 561884-562426 (Clockwise)

Preceding gene: 120597352

Following gene: 120597355

Centisome position: 11.93

GC content: 43.83

Gene sequence:

>543_bases
ATGAGCTTTGAGTTTGGGTGGTATAGCGCCGCTCGGCGCAGTGAGTCGCCACATTTTAATCAACGCCCTGAGGCTGAAGT
GAGTTTATTGGTGATCCACAATATTAGCTTGCCGGCGGGCTGTTTTGGTTTACCTTATATTGATCAATTATTTAAAGGGT
GTTTAGATACCAGTGCCGATGACAGTTTTACTGAGTTAGCGGGCTTGCAAGTATCAGCACATTTTTTGATCCGCCGTGAT
GGTGAGTGTGTACAATATGTCAGTTGTCACGATAGGGCATGGCACGCTGGCGTATCTTGCTATGGCGAAAGGGAAAATTG
TAATGATTTTTCTATTGGTATTGAGCTTGAAGGGACGGATACTGAGCCTTACGTTGATGCCCAATATGTGCGTTTGGTTG
AGTTAACACAGGCATTGTTGATTGAATATCCATTATTAAATACTAAGCGGATTGTTGGGCACTGTGATATAGCACCATTA
CGTAAAACAGATCCCGGGCAAAGTTTTGATTGGGAAAGATATTTAAGGGCGCTGCGCTGTTAG

Upstream 100 bases:

>100_bases
GTCCTGCTAGAGCGTCGCTTTGCTCCTTCTAGAATCTATCCGCTGCGTTCTTTGCAGCGTCCTCGCAGGGCGAAGCCCGT
CCTGCTTTTAAGGAGTAAAA

Downstream 100 bases:

>100_bases
GGCGGACTTCGTCCTGCTAGAGCGTCGCTGCGCTCCTTCTAGAATCTAGAGTCTAGAACTTCGAGCTTAAGTACATAAAG
CTGGATCCCCGCGTTCGCGG

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 180; Mature: 179

Protein sequence:

>180_residues
MSFEFGWYSAARRSESPHFNQRPEAEVSLLVIHNISLPAGCFGLPYIDQLFKGCLDTSADDSFTELAGLQVSAHFLIRRD
GECVQYVSCHDRAWHAGVSCYGERENCNDFSIGIELEGTDTEPYVDAQYVRLVELTQALLIEYPLLNTKRIVGHCDIAPL
RKTDPGQSFDWERYLRALRC

Sequences:

>Translated_180_residues
MSFEFGWYSAARRSESPHFNQRPEAEVSLLVIHNISLPAGCFGLPYIDQLFKGCLDTSADDSFTELAGLQVSAHFLIRRD
GECVQYVSCHDRAWHAGVSCYGERENCNDFSIGIELEGTDTEPYVDAQYVRLVELTQALLIEYPLLNTKRIVGHCDIAPL
RKTDPGQSFDWERYLRALRC
>Mature_179_residues
SFEFGWYSAARRSESPHFNQRPEAEVSLLVIHNISLPAGCFGLPYIDQLFKGCLDTSADDSFTELAGLQVSAHFLIRRDG
ECVQYVSCHDRAWHAGVSCYGERENCNDFSIGIELEGTDTEPYVDAQYVRLVELTQALLIEYPLLNTKRIVGHCDIAPLR
KTDPGQSFDWERYLRALRC

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786300, Length=175, Percent_Identity=61.1428571428571, Blast_Score=207, Evalue=3e-55,
Organism=Escherichia coli, GI1787092, Length=130, Percent_Identity=35.3846153846154, Blast_Score=74, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 20442; Mature: 20311

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.4 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
4.5 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFEFGWYSAARRSESPHFNQRPEAEVSLLVIHNISLPAGCFGLPYIDQLFKGCLDTSAD
CCCCCCCHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCC
DSFTELAGLQVSAHFLIRRDGECVQYVSCHDRAWHAGVSCYGERENCNDFSIGIELEGTD
CCHHHHHCCEEEEEEEEECCCCEEEEEHHCCCHHHCCCEEECCCCCCCEEEEEEEEECCC
TEPYVDAQYVRLVELTQALLIEYPLLNTKRIVGHCDIAPLRKTDPGQSFDWERYLRALRC
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
SFEFGWYSAARRSESPHFNQRPEAEVSLLVIHNISLPAGCFGLPYIDQLFKGCLDTSAD
CCCCCCHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCC
DSFTELAGLQVSAHFLIRRDGECVQYVSCHDRAWHAGVSCYGERENCNDFSIGIELEGTD
CCHHHHHCCEEEEEEEEECCCCEEEEEHHCCCHHHCCCEEECCCCCCCEEEEEEEEECCC
TEPYVDAQYVRLVELTQALLIEYPLLNTKRIVGHCDIAPLRKTDPGQSFDWERYLRALRC
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383940 [H]