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The map label for this gene is epsJ [H]

Identifier: 120596890

GI number: 120596890

Start: 71321

End: 72280

Strand: Direct

Name: epsJ [H]

Synonym: Sputw3181_0057

Alternate gene names: 120596890

Gene position: 71321-72280 (Clockwise)

Preceding gene: 120596889

Following gene: 120596893

Centisome position: 1.51

GC content: 31.77

Gene sequence:

>960_bases
ATGACAAAAATGCAACCTATAATATCAATTATTATTCCTATCTATAAGGTTGAGAGGTTTTTAGAAAGGTGTTTGAATAG
TGTGCTGGCCCAAACCTATAATGATTTTGAGGTTATTTGTATTAACGATGGTTCACCAGATAATTGTGACTTAATTTTAG
CAGAATATGAAAAAAATGATAATAGAATTAAAGTCATTAACCAAGTCAACCAAGGTCCATCAGTTGCTCGTAATGCTGGT
TTAAGCTCGGCTAGAGGGGATTATGTATATTTCATCGATCCTGATGATGCGATACAACCACAGACATTAGAAATAGCTTT
CCTTTTGGCAAAAAAACATCAAGCTGATTTAGTCGTGTGGCAAAGGGTTTACTCTAATGGCTTAGGGAAACCAGATAAGA
TATTTGATCTCAAAAAAATAGATGCAGTAATAATCACAGATCCTTTATATCATAAAAGTAAACGTCATAATAAAATAGGT
TCAGTTGTATGGAATAAACTTTATAAAAGAGAATTGCTAAAAGATATATTTTTCATCCCTGGAATACATTATGAAGATAT
TTCATTTAGTTTTTCTGTCATCGCTAAACGTCCTAAAATCGTAATTATTCCAACGCCGTTATATTTATATACGAAGTTTG
AAGAGTCGATAACGCAACAAAAACCGACGTTGAAAATTATTAATGATTATCATATAGCAATAGATTCAATATATGAAACT
TATAAAAAAAGTATGGGCAGCAAGGAATTAAAACATATAAAAAATAACCTAATGCGACCTCTTTTAAGTTATCAATTTAA
AAAGTGTAAACAAGCAGATCAAGAACAGAGATCTGCAATGTTGCAAGCTTTTGCTGAAGAGTTAAGAGATCTAGATGCTA
AGGGCTTGCTTACATGGCGTGGACATCGTATTTATCTGTATATAGCTTATAAAAAGTTAATGAGAACAGCTCGGCTATAA

Upstream 100 bases:

>100_bases
TTATTGTATAATATTTTGAGTGCGAAATTATTTTTATGATATTCGATGGTCTTGTCAGTAGTTGAAAACCAAATTTATTA
GGATTTAAAGGGATGTGGTA

Downstream 100 bases:

>100_bases
TAAGATGAAATCAACCTAGTTAGCTTTAGATCTACACTAGGATGGATTTTCTAGTCGAGTGACTTTAATAATTTGATTTT
TATGCTGAAATGTATAAATA

Product: glycosyl transferase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MTKMQPIISIIIPIYKVERFLERCLNSVLAQTYNDFEVICINDGSPDNCDLILAEYEKNDNRIKVINQVNQGPSVARNAG
LSSARGDYVYFIDPDDAIQPQTLEIAFLLAKKHQADLVVWQRVYSNGLGKPDKIFDLKKIDAVIITDPLYHKSKRHNKIG
SVVWNKLYKRELLKDIFFIPGIHYEDISFSFSVIAKRPKIVIIPTPLYLYTKFEESITQQKPTLKIINDYHIAIDSIYET
YKKSMGSKELKHIKNNLMRPLLSYQFKKCKQADQEQRSAMLQAFAEELRDLDAKGLLTWRGHRIYLYIAYKKLMRTARL

Sequences:

>Translated_319_residues
MTKMQPIISIIIPIYKVERFLERCLNSVLAQTYNDFEVICINDGSPDNCDLILAEYEKNDNRIKVINQVNQGPSVARNAG
LSSARGDYVYFIDPDDAIQPQTLEIAFLLAKKHQADLVVWQRVYSNGLGKPDKIFDLKKIDAVIITDPLYHKSKRHNKIG
SVVWNKLYKRELLKDIFFIPGIHYEDISFSFSVIAKRPKIVIIPTPLYLYTKFEESITQQKPTLKIINDYHIAIDSIYET
YKKSMGSKELKHIKNNLMRPLLSYQFKKCKQADQEQRSAMLQAFAEELRDLDAKGLLTWRGHRIYLYIAYKKLMRTARL
>Mature_318_residues
TKMQPIISIIIPIYKVERFLERCLNSVLAQTYNDFEVICINDGSPDNCDLILAEYEKNDNRIKVINQVNQGPSVARNAGL
SSARGDYVYFIDPDDAIQPQTLEIAFLLAKKHQADLVVWQRVYSNGLGKPDKIFDLKKIDAVIITDPLYHKSKRHNKIGS
VVWNKLYKRELLKDIFFIPGIHYEDISFSFSVIAKRPKIVIIPTPLYLYTKFEESITQQKPTLKIINDYHIAIDSIYETY
KKSMGSKELKHIKNNLMRPLLSYQFKKCKQADQEQRSAMLQAFAEELRDLDAKGLLTWRGHRIYLYIAYKKLMRTARL

Specific function: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790044, Length=230, Percent_Identity=28.2608695652174, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788372, Length=188, Percent_Identity=28.7234042553192, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787259, Length=94, Percent_Identity=38.2978723404255, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 37114; Mature: 36983

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKMQPIISIIIPIYKVERFLERCLNSVLAQTYNDFEVICINDGSPDNCDLILAEYEKND
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEEEECCCC
NRIKVINQVNQGPSVARNAGLSSARGDYVYFIDPDDAIQPQTLEIAFLLAKKHQADLVVW
CEEEEEECCCCCCHHHHHCCCCCCCCCEEEEECCCCCCCCCHHEEEEEEHHCCCHHHHHH
QRVYSNGLGKPDKIFDLKKIDAVIITDPLYHKSKRHNKIGSVVWNKLYKRELLKDIFFIP
HHHHHCCCCCCHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GIHYEDISFSFSVIAKRPKIVIIPTPLYLYTKFEESITQQKPTLKIINDYHIAIDSIYET
CCCCCCCCEEHHEEECCCEEEEEECCHHEEEHHHHHHHCCCCCEEEECHHHHHHHHHHHH
YKKSMGSKELKHIKNNLMRPLLSYQFKKCKQADQEQRSAMLQAFAEELRDLDAKGLLTWR
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEC
GHRIYLYIAYKKLMRTARL
CCEEEEEHHHHHHHHHHCC
>Mature Secondary Structure 
TKMQPIISIIIPIYKVERFLERCLNSVLAQTYNDFEVICINDGSPDNCDLILAEYEKND
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEEEECCCC
NRIKVINQVNQGPSVARNAGLSSARGDYVYFIDPDDAIQPQTLEIAFLLAKKHQADLVVW
CEEEEEECCCCCCHHHHHCCCCCCCCCEEEEECCCCCCCCCHHEEEEEEHHCCCHHHHHH
QRVYSNGLGKPDKIFDLKKIDAVIITDPLYHKSKRHNKIGSVVWNKLYKRELLKDIFFIP
HHHHHCCCCCCHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GIHYEDISFSFSVIAKRPKIVIIPTPLYLYTKFEESITQQKPTLKIINDYHIAIDSIYET
CCCCCCCCEEHHEEECCCEEEEEECCHHEEEHHHHHHHCCCCCEEEECHHHHHHHHHHHH
YKKSMGSKELKHIKNNLMRPLLSYQFKKCKQADQEQRSAMLQAFAEELRDLDAKGLLTWR
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEC
GHRIYLYIAYKKLMRTARL
CCEEEEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377 [H]