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The map label for this gene is leuA [H]

Identifier: 119946959

GI number: 119946959

Start: 4142979

End: 4144715

Strand: Direct

Name: leuA [H]

Synonym: Ping_3353

Alternate gene names: 119946959

Gene position: 4142979-4144715 (Clockwise)

Preceding gene: 119946957

Following gene: 119946965

Centisome position: 90.86

GC content: 46.92

Gene sequence:

>1737_bases
GTGTCGAATATCAACTCTAGCTTTAACCATAAAAAATATCGCGCCTATCCATCCATTCCAAAAACCGATAGACGCTGGCC
AGATCAAAGTATCAAAGCAGCACCTCTGTGGTGTAGTGTAGATTTACGCGATGGTAATCAAGCTCTGATTGAACCTATGA
GCCCAAGCCAAAAGCTTGATATGTTTAAACTGTTAGTTGATGTCGGCTTTAAAGAGATTGAAGTTGGTTTTCCCGCAGCA
TCGGCACCTGATTTTGATTTTGTGCGTCAATTAATTGAACAGGATTTAATTCCAGACGACGTCACTATTCAAGTATTAAC
CCAGGCACGCGAGCCTTTAATAGCACGCAGCTTTGCCGCTTTAAAAGGCGCAAAAAAAGCCATAGTGCACTTATATAACT
CAACCTCAACGGTGCAGCGAGAACAGGTATTTAAAAAATCGCGCGATGAAATTAAAACCCTTGCCATACAAGGTGCGCGA
TGGATGCAGGAATTTGCCGCCGCTCAATCCGAGACAAAGTGGTCGTTTCAATACTCGCCCGAGAGTTTTACCGGCACCGA
AAGGGAGTATGCCTTAGAGGTGTGTAATGCTGTCATTAACGTTTGGCAGCCGACACCTGACAACAAGGTAATTATTAATT
TACCCTCGACGGTTGAGACATCAACGCCTAACGTCTACGCAGATCAAATTGAATGGATCTGCGACAATATCAGCCACCGC
CAATCGGTCAGCATCAGCTTACATACTCACAATGACCGCGGCTGTGGCGTCGCTGCCTCTGAGCTCGGTGTACTGGCCGG
TGCTGATCGTATCGAAGGGACATTACTTGGCAACGGTGAACGCACGGGTAATATGGATATAGTGACGATGGCGATGAATA
TATATAGTCAGGGCATTGATCCAAAACTCGATTTTTCCGATATGGATCGTATTATTGCAACCATTGAGCGCTGCACACAA
TTAGCCGTACACCCTCGTCATCCCTACGCCGGTGAATTAGTTTTTGCAGCATTTTCTGGCAGCCATCAGGATGCGATTAA
CAAATGTATGGCGATTGATGCGGCGCAGCGAATAGAGAAACCAGAAACACAATGGCAAGTGGCTTATCTGCCGATTGATC
CGCGCGATTTAGGGCGAACTTATCAGCAGGTTATTCGCATTAATAGCCAGTCAGGAAAAGGCGGAGTCGCCTATATACTG
GAGCAGGACTACGGTATACAAATGCCGCGCTGGATGCAGGTTGACTTTAGTCCCCATGTACAACAGCATGCCGAACGCTC
AGAAAGTGAAGTTTCAGCGACTATTATTCACGATATTTTTTCCAGTACTTATTTAGCAGCACAGAAAACCGCCAGTATCG
ACAACTACCAGCTTAACCGTCAAAGTAACGCAGATAGTTTAGAGGCGCAGATACTCGATACTAATAACGGTGCAGTGACT
ATTAATGGTCAGGGTAAAGGGGTTCTGGACGCTTTTGTCAAAGGGTTAAGTGCGTCAATCGGAAGTGATATTGTCTTAAT
CGATTATAGCGAGCATGCCCTGCCGAGCAAAAAAGGGGCTGAAAATGAACAGGCCGAAGCGATCGCCTATGTGCAACTTA
GCGTTCAAGGCGCGCGTTACTGCGCGGCAGCCAAGTGCCACGATATAGTCACCGCTTCTTTGCAGGCAGTCCTTAATGCC
GTTACCCGCAGTGGAGCTACCTGCCAGCGCAGCAAGGCAGAATTAGCCGCAGTGTAG

Upstream 100 bases:

>100_bases
GCGAAGTTTCTTACCGGAAACGGGCGGTTAACCCCTTACCAAGGTGCACCAACTCGCAAATTCATCTCTCCACTGCCAAT
GATTTACAAGGAAATGACTC

Downstream 100 bases:

>100_bases
CCCTACTCTCTATGAGTTAAAATTCTCAAAAAAAATGAATTATTCACCCTGCAACAGGGTTGGATAATTCATTTAAAGCT
CAAATATGGTGCTCATCAAC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 578; Mature: 577

Protein sequence:

>578_residues
MSNINSSFNHKKYRAYPSIPKTDRRWPDQSIKAAPLWCSVDLRDGNQALIEPMSPSQKLDMFKLLVDVGFKEIEVGFPAA
SAPDFDFVRQLIEQDLIPDDVTIQVLTQAREPLIARSFAALKGAKKAIVHLYNSTSTVQREQVFKKSRDEIKTLAIQGAR
WMQEFAAAQSETKWSFQYSPESFTGTEREYALEVCNAVINVWQPTPDNKVIINLPSTVETSTPNVYADQIEWICDNISHR
QSVSISLHTHNDRGCGVAASELGVLAGADRIEGTLLGNGERTGNMDIVTMAMNIYSQGIDPKLDFSDMDRIIATIERCTQ
LAVHPRHPYAGELVFAAFSGSHQDAINKCMAIDAAQRIEKPETQWQVAYLPIDPRDLGRTYQQVIRINSQSGKGGVAYIL
EQDYGIQMPRWMQVDFSPHVQQHAERSESEVSATIIHDIFSSTYLAAQKTASIDNYQLNRQSNADSLEAQILDTNNGAVT
INGQGKGVLDAFVKGLSASIGSDIVLIDYSEHALPSKKGAENEQAEAIAYVQLSVQGARYCAAAKCHDIVTASLQAVLNA
VTRSGATCQRSKAELAAV

Sequences:

>Translated_578_residues
MSNINSSFNHKKYRAYPSIPKTDRRWPDQSIKAAPLWCSVDLRDGNQALIEPMSPSQKLDMFKLLVDVGFKEIEVGFPAA
SAPDFDFVRQLIEQDLIPDDVTIQVLTQAREPLIARSFAALKGAKKAIVHLYNSTSTVQREQVFKKSRDEIKTLAIQGAR
WMQEFAAAQSETKWSFQYSPESFTGTEREYALEVCNAVINVWQPTPDNKVIINLPSTVETSTPNVYADQIEWICDNISHR
QSVSISLHTHNDRGCGVAASELGVLAGADRIEGTLLGNGERTGNMDIVTMAMNIYSQGIDPKLDFSDMDRIIATIERCTQ
LAVHPRHPYAGELVFAAFSGSHQDAINKCMAIDAAQRIEKPETQWQVAYLPIDPRDLGRTYQQVIRINSQSGKGGVAYIL
EQDYGIQMPRWMQVDFSPHVQQHAERSESEVSATIIHDIFSSTYLAAQKTASIDNYQLNRQSNADSLEAQILDTNNGAVT
INGQGKGVLDAFVKGLSASIGSDIVLIDYSEHALPSKKGAENEQAEAIAYVQLSVQGARYCAAAKCHDIVTASLQAVLNA
VTRSGATCQRSKAELAAV
>Mature_577_residues
SNINSSFNHKKYRAYPSIPKTDRRWPDQSIKAAPLWCSVDLRDGNQALIEPMSPSQKLDMFKLLVDVGFKEIEVGFPAAS
APDFDFVRQLIEQDLIPDDVTIQVLTQAREPLIARSFAALKGAKKAIVHLYNSTSTVQREQVFKKSRDEIKTLAIQGARW
MQEFAAAQSETKWSFQYSPESFTGTEREYALEVCNAVINVWQPTPDNKVIINLPSTVETSTPNVYADQIEWICDNISHRQ
SVSISLHTHNDRGCGVAASELGVLAGADRIEGTLLGNGERTGNMDIVTMAMNIYSQGIDPKLDFSDMDRIIATIERCTQL
AVHPRHPYAGELVFAAFSGSHQDAINKCMAIDAAQRIEKPETQWQVAYLPIDPRDLGRTYQQVIRINSQSGKGGVAYILE
QDYGIQMPRWMQVDFSPHVQQHAERSESEVSATIIHDIFSSTYLAAQKTASIDNYQLNRQSNADSLEAQILDTNNGAVTI
NGQGKGVLDAFVKGLSASIGSDIVLIDYSEHALPSKKGAENEQAEAIAYVQLSVQGARYCAAAKCHDIVTASLQAVLNAV
TRSGATCQRSKAELAAV

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=311, Percent_Identity=27.9742765273312, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324682, Length=527, Percent_Identity=46.2998102466793, Blast_Score=506, Evalue=1e-144,
Organism=Saccharomyces cerevisiae, GI6324225, Length=579, Percent_Identity=43.8687392055268, Blast_Score=504, Evalue=1e-143,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 63745; Mature: 63614

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNINSSFNHKKYRAYPSIPKTDRRWPDQSIKAAPLWCSVDLRDGNQALIEPMSPSQKLD
CCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEECCCCCCHHHH
MFKLLVDVGFKEIEVGFPAASAPDFDFVRQLIEQDLIPDDVTIQVLTQAREPLIARSFAA
HHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHH
LKGAKKAIVHLYNSTSTVQREQVFKKSRDEIKTLAIQGARWMQEFAAAQSETKWSFQYSP
HHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
ESFTGTEREYALEVCNAVINVWQPTPDNKVIINLPSTVETSTPNVYADQIEWICDNISHR
CCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCEEHHHHHHHHHCCCCC
QSVSISLHTHNDRGCGVAASELGVLAGADRIEGTLLGNGERTGNMDIVTMAMNIYSQGID
CEEEEEEEECCCCCCCCCHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEEEHHHHHCCCC
PKLDFSDMDRIIATIERCTQLAVHPRHPYAGELVFAAFSGSHQDAINKCMAIDAAQRIEK
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCC
PETQWQVAYLPIDPRDLGRTYQQVIRINSQSGKGGVAYILEQDYGIQMPRWMQVDFSPHV
CCCCEEEEEEECCHHHHHHHHHHHHEECCCCCCCCEEEEEECCCCCCCCCEEEECCCHHH
QQHAERSESEVSATIIHDIFSSTYLAAQKTASIDNYQLNRQSNADSLEAQILDTNNGAVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEEEEECCCCEEE
INGQGKGVLDAFVKGLSASIGSDIVLIDYSEHALPSKKGAENEQAEAIAYVQLSVQGARY
ECCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEEEEECCCHH
CAAAKCHDIVTASLQAVLNAVTRSGATCQRSKAELAAV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCC
>Mature Secondary Structure 
SNINSSFNHKKYRAYPSIPKTDRRWPDQSIKAAPLWCSVDLRDGNQALIEPMSPSQKLD
CCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEECCCCCCHHHH
MFKLLVDVGFKEIEVGFPAASAPDFDFVRQLIEQDLIPDDVTIQVLTQAREPLIARSFAA
HHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHH
LKGAKKAIVHLYNSTSTVQREQVFKKSRDEIKTLAIQGARWMQEFAAAQSETKWSFQYSP
HHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
ESFTGTEREYALEVCNAVINVWQPTPDNKVIINLPSTVETSTPNVYADQIEWICDNISHR
CCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCEEHHHHHHHHHCCCCC
QSVSISLHTHNDRGCGVAASELGVLAGADRIEGTLLGNGERTGNMDIVTMAMNIYSQGID
CEEEEEEEECCCCCCCCCHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEEEHHHHHCCCC
PKLDFSDMDRIIATIERCTQLAVHPRHPYAGELVFAAFSGSHQDAINKCMAIDAAQRIEK
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCC
PETQWQVAYLPIDPRDLGRTYQQVIRINSQSGKGGVAYILEQDYGIQMPRWMQVDFSPHV
CCCCEEEEEEECCHHHHHHHHHHHHEECCCCCCCCEEEEEECCCCCCCCCEEEECCCHHH
QQHAERSESEVSATIIHDIFSSTYLAAQKTASIDNYQLNRQSNADSLEAQILDTNNGAVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEEEEECCCCEEE
INGQGKGVLDAFVKGLSASIGSDIVLIDYSEHALPSKKGAENEQAEAIAYVQLSVQGARY
ECCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEEEEECCCHH
CAAAKCHDIVTASLQAVLNAVTRSGATCQRSKAELAAV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA