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The map label for this gene is dusB [H]

Identifier: 119946821

GI number: 119946821

Start: 3970912

End: 3971871

Strand: Direct

Name: dusB [H]

Synonym: Ping_3215

Alternate gene names: 119946821

Gene position: 3970912-3971871 (Clockwise)

Preceding gene: 119946820

Following gene: 119946822

Centisome position: 87.09

GC content: 43.33

Gene sequence:

>960_bases
ATGCGCATCGGGCAGCATCAACTAGATAACAAAATCATTTTAGCACCCATGGCAGGGGTAACTGATCGTCCCTTCCGTGA
GCTTTGCCGAAAACAAGGTGCAGGACTTGCAGTGTCCGAGATGCTGTCATCGAATCCGCGTGTATGGAACAGCAGGAAGT
CGAAGTTAAGAATGGACTACCATAATGAATCTGGCATCAGATCAGTGCAAATTGCTGGCTCAGAGCCTTTGGCAATGGCG
ACAGCAGCACAGTTGAGTGTGCAACAAGGCGCTCAGATTATTGATATCAATATGGGTTGTCCCGCTAAAAAAGTGAATAA
GAAAAAAGCAGGCTCAGCATTATTACCTCACCCAGAACTTGTGCGAGCAATTTTGGAAACCGTGGTTGATGCCGTTGATG
TGCCTGTCACTTTAAAAATTCGCACTGGCTGGGATCCCGACAGTAGAAACGGTCTGGAAATCGCACGAATAGCAGAACAA
TCGGGCATCCAAGCACTTGCAGTGCACGGTCGTACTAGGCAATGTTTTTATAATGGATTTGCTGAATACGACACCATAAA
AAAAATTAAACAAAATGTGACTATTCCTGTGATTGCAAATGGTGATATCACCTCAGTGGAAAAGGCAAAGTTTGTGCTTG
ATTACACTGGCGCTGACGCCATTATGATTGGACGAGGAGCACAAGGTGCTCCTTGGATATTCAATGAAATTAATCACTAT
CTAACAACCGGAGAGCAACTTTCCTCTCCGGCTAAAGCGGAAATTGCCCATATCCTTCTTGGTCATGTACAAGCTTTACA
TGGTTTTTACGGGGAAGTAATGGGACCTAGAATTGCCAGAAAGCATGTTGGATGGTATTTAAAAGAGCAAGATCAGTTAG
GTCACTTTAAGCGACAATTCAATGCAATTGACTGCCCGTATGAGCAATTAAAAACATTAGAAACTTACTTAAATAAATAA

Upstream 100 bases:

>100_bases
TGCAAGCAAAATAATTATAAAATGCTTAATTAATAACCTTTCTTCTCGTTGGAAAAATGCGTACACTACGCCGCCTTGAC
CAAGAGAGATTGCTTAATAT

Downstream 100 bases:

>100_bases
AGAGTTAATAGCTATGTTTGAACAAAAGATTTCGTCAGAAGCACTTACAACAACAACTAGTATTCCTGCTACAGGGCAAA
TAACTCAGCGACCACTTCGT

Product: tRNA-dihydrouridine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MRIGQHQLDNKIILAPMAGVTDRPFRELCRKQGAGLAVSEMLSSNPRVWNSRKSKLRMDYHNESGIRSVQIAGSEPLAMA
TAAQLSVQQGAQIIDINMGCPAKKVNKKKAGSALLPHPELVRAILETVVDAVDVPVTLKIRTGWDPDSRNGLEIARIAEQ
SGIQALAVHGRTRQCFYNGFAEYDTIKKIKQNVTIPVIANGDITSVEKAKFVLDYTGADAIMIGRGAQGAPWIFNEINHY
LTTGEQLSSPAKAEIAHILLGHVQALHGFYGEVMGPRIARKHVGWYLKEQDQLGHFKRQFNAIDCPYEQLKTLETYLNK

Sequences:

>Translated_319_residues
MRIGQHQLDNKIILAPMAGVTDRPFRELCRKQGAGLAVSEMLSSNPRVWNSRKSKLRMDYHNESGIRSVQIAGSEPLAMA
TAAQLSVQQGAQIIDINMGCPAKKVNKKKAGSALLPHPELVRAILETVVDAVDVPVTLKIRTGWDPDSRNGLEIARIAEQ
SGIQALAVHGRTRQCFYNGFAEYDTIKKIKQNVTIPVIANGDITSVEKAKFVLDYTGADAIMIGRGAQGAPWIFNEINHY
LTTGEQLSSPAKAEIAHILLGHVQALHGFYGEVMGPRIARKHVGWYLKEQDQLGHFKRQFNAIDCPYEQLKTLETYLNK
>Mature_319_residues
MRIGQHQLDNKIILAPMAGVTDRPFRELCRKQGAGLAVSEMLSSNPRVWNSRKSKLRMDYHNESGIRSVQIAGSEPLAMA
TAAQLSVQQGAQIIDINMGCPAKKVNKKKAGSALLPHPELVRAILETVVDAVDVPVTLKIRTGWDPDSRNGLEIARIAEQ
SGIQALAVHGRTRQCFYNGFAEYDTIKKIKQNVTIPVIANGDITSVEKAKFVLDYTGADAIMIGRGAQGAPWIFNEINHY
LTTGEQLSSPAKAEIAHILLGHVQALHGFYGEVMGPRIARKHVGWYLKEQDQLGHFKRQFNAIDCPYEQLKTLETYLNK

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=231, Percent_Identity=32.034632034632, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI239788462, Length=231, Percent_Identity=32.034632034632, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI31742496, Length=225, Percent_Identity=32.8888888888889, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI40807366, Length=227, Percent_Identity=30.8370044052863, Blast_Score=101, Evalue=9e-22,
Organism=Homo sapiens, GI8923374, Length=245, Percent_Identity=32.2448979591837, Blast_Score=98, Evalue=8e-21,
Organism=Escherichia coli, GI1789660, Length=317, Percent_Identity=64.9842271293375, Blast_Score=427, Evalue=1e-121,
Organism=Escherichia coli, GI1788462, Length=231, Percent_Identity=35.0649350649351, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI145693211, Length=243, Percent_Identity=26.7489711934156, Blast_Score=80, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17543114, Length=242, Percent_Identity=31.404958677686, Blast_Score=107, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI25144369, Length=214, Percent_Identity=31.3084112149533, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17510279, Length=243, Percent_Identity=33.3333333333333, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17507177, Length=252, Percent_Identity=28.5714285714286, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323560, Length=248, Percent_Identity=30.241935483871, Blast_Score=100, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6323433, Length=275, Percent_Identity=28.7272727272727, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323437, Length=229, Percent_Identity=27.9475982532751, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24585320, Length=256, Percent_Identity=30.46875, Blast_Score=118, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24580595, Length=256, Percent_Identity=31.25, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI19920448, Length=256, Percent_Identity=31.25, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI45549423, Length=244, Percent_Identity=31.1475409836066, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19921524, Length=243, Percent_Identity=29.6296296296296, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35290; Mature: 35290

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGQHQLDNKIILAPMAGVTDRPFRELCRKQGAGLAVSEMLSSNPRVWNSRKSKLRMDY
CCCCCHHCCCEEEEEECCCCCCCHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCEEEEE
HNESGIRSVQIAGSEPLAMATAAQLSVQQGAQIIDINMGCPAKKVNKKKAGSALLPHPEL
CCCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCCCCHHH
VRAILETVVDAVDVPVTLKIRTGWDPDSRNGLEIARIAEQSGIQALAVHGRTRQCFYNGF
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHCCCEEEEECCCCHHHHHCCH
AEYDTIKKIKQNVTIPVIANGDITSVEKAKFVLDYTGADAIMIGRGAQGAPWIFNEINHY
HHHHHHHHHHHCCEEEEEECCCCCCHHHEEEEEEECCCCEEEEECCCCCCCHHHHHHHHH
LTTGEQLSSPAKAEIAHILLGHVQALHGFYGEVMGPRIARKHVGWYLKEQDQLGHFKRQF
HCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCHHHHHHHHHHC
NAIDCPYEQLKTLETYLNK
CCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MRIGQHQLDNKIILAPMAGVTDRPFRELCRKQGAGLAVSEMLSSNPRVWNSRKSKLRMDY
CCCCCHHCCCEEEEEECCCCCCCHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCEEEEE
HNESGIRSVQIAGSEPLAMATAAQLSVQQGAQIIDINMGCPAKKVNKKKAGSALLPHPEL
CCCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCCCCHHH
VRAILETVVDAVDVPVTLKIRTGWDPDSRNGLEIARIAEQSGIQALAVHGRTRQCFYNGF
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHCCCEEEEECCCCHHHHHCCH
AEYDTIKKIKQNVTIPVIANGDITSVEKAKFVLDYTGADAIMIGRGAQGAPWIFNEINHY
HHHHHHHHHHHCCEEEEEECCCCCCHHHEEEEEEECCCCEEEEECCCCCCCHHHHHHHHH
LTTGEQLSSPAKAEIAHILLGHVQALHGFYGEVMGPRIARKHVGWYLKEQDQLGHFKRQF
HCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCHHHHHHHHHHC
NAIDCPYEQLKTLETYLNK
CCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]