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The map label for this gene is thiE [H]

Identifier: 119946028

GI number: 119946028

Start: 2889297

End: 2889914

Strand: Reverse

Name: thiE [H]

Synonym: Ping_2372

Alternate gene names: 119946028

Gene position: 2889914-2889297 (Counterclockwise)

Preceding gene: 119946029

Following gene: 119946027

Centisome position: 63.38

GC content: 45.31

Gene sequence:

>618_bases
GTGAACCCCTATCGTTTATATTTAGTGACCGATGACCAGCAGAATCTTGAAACATTGAAAAGGGTGGTTGCTGAAGCAGT
TGTCGGTGGTGTGACTATGGTCCAAGTTCGGGAAAAGCAGGGTGATCTTCGCGTTTTTATAGAAAGGGTGTTAGCGGTAA
AGGCAATTTTACACGGGACGGGCGTACCTTTGATCGTCAATGACAGAGTGGATGTTGCACTGGCCGTTGATGCTGATGGT
GTACACTTGGGTCAATCGGATATGCCGGTAGAAACCGCCCGCAGACTAATAGGAAAAGATAAATTACTTGGCCTGTCTAT
TGAGAATCCGCAGCAGCTTATTGAAGCGGAATCTTTGCCCGTCGATTATTTGGGGTTGAGTGCTATTTTTTCTACAGCAA
CAAAAACCAACATCAAGAAAGAATGGGGTATTGAGGGGCTTACCAATGCCGTAGCGCAAAGCTCCTTGCCGATAGTTGCT
ATTGGTGGTATTAATGTTTCAAATCTTGATGAGGTTATTGCAAGCAATGTTGCTGGCGTGGCATTAGTCTCGGCTATTTG
TCATGCACCGTCGCCAAAACAAGCGGCAATAAATTTGCTCAAACAGATGGGGAAATAG

Upstream 100 bases:

>100_bases
AGATGCTTTATATCAGCTCGATGAAGCGACGCTGATTCAGCGTTTAAAATTATCCTAGCCTGTATCAAAAAATTTGGAGA
ATAAAGAGTTGGAGAAAAAA

Downstream 100 bases:

>100_bases
CTGATAAAGGCGAGAAGGTGGGAAGGTGGGAAGGCTGTCGGCTTTCGGCTGTCAGCTCTAAACTCGAAGCTCGAAGGCTC
TAAACTCGAAGACTCGATTA

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MNPYRLYLVTDDQQNLETLKRVVAEAVVGGVTMVQVREKQGDLRVFIERVLAVKAILHGTGVPLIVNDRVDVALAVDADG
VHLGQSDMPVETARRLIGKDKLLGLSIENPQQLIEAESLPVDYLGLSAIFSTATKTNIKKEWGIEGLTNAVAQSSLPIVA
IGGINVSNLDEVIASNVAGVALVSAICHAPSPKQAAINLLKQMGK

Sequences:

>Translated_205_residues
MNPYRLYLVTDDQQNLETLKRVVAEAVVGGVTMVQVREKQGDLRVFIERVLAVKAILHGTGVPLIVNDRVDVALAVDADG
VHLGQSDMPVETARRLIGKDKLLGLSIENPQQLIEAESLPVDYLGLSAIFSTATKTNIKKEWGIEGLTNAVAQSSLPIVA
IGGINVSNLDEVIASNVAGVALVSAICHAPSPKQAAINLLKQMGK
>Mature_205_residues
MNPYRLYLVTDDQQNLETLKRVVAEAVVGGVTMVQVREKQGDLRVFIERVLAVKAILHGTGVPLIVNDRVDVALAVDADG
VHLGQSDMPVETARRLIGKDKLLGLSIENPQQLIEAESLPVDYLGLSAIFSTATKTNIKKEWGIEGLTNAVAQSSLPIVA
IGGINVSNLDEVIASNVAGVALVSAICHAPSPKQAAINLLKQMGK

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=205, Percent_Identity=31.219512195122, Blast_Score=72, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6325042, Length=210, Percent_Identity=40, Blast_Score=129, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 21837; Mature: 21837

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPYRLYLVTDDQQNLETLKRVVAEAVVGGVTMVQVREKQGDLRVFIERVLAVKAILHGT
CCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCC
GVPLIVNDRVDVALAVDADGVHLGQSDMPVETARRLIGKDKLLGLSIENPQQLIEAESLP
CCCEEECCCCEEEEEECCCCCEECCCCCCHHHHHHHHCCCCEEEECCCCHHHHHHHHCCC
VDYLGLSAIFSTATKTNIKKEWGIEGLTNAVAQSSLPIVAIGGINVSNLDEVIASNVAGV
HHHHHHHHHHHHHHHCCCHHHHCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHH
ALVSAICHAPSPKQAAINLLKQMGK
HHHHHHHCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MNPYRLYLVTDDQQNLETLKRVVAEAVVGGVTMVQVREKQGDLRVFIERVLAVKAILHGT
CCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCC
GVPLIVNDRVDVALAVDADGVHLGQSDMPVETARRLIGKDKLLGLSIENPQQLIEAESLP
CCCEEECCCCEEEEEECCCCCEECCCCCCHHHHHHHHCCCCEEEECCCCHHHHHHHHCCC
VDYLGLSAIFSTATKTNIKKEWGIEGLTNAVAQSSLPIVAIGGINVSNLDEVIASNVAGV
HHHHHHHHHHHHHHHCCCHHHHCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHH
ALVSAICHAPSPKQAAINLLKQMGK
HHHHHHHCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA