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The map label for this gene is thiM

Identifier: 119946029

GI number: 119946029

Start: 2889957

End: 2890760

Strand: Reverse

Name: thiM

Synonym: Ping_2373

Alternate gene names: 119946029

Gene position: 2890760-2889957 (Counterclockwise)

Preceding gene: 119946030

Following gene: 119946028

Centisome position: 63.4

GC content: 47.39

Gene sequence:

>804_bases
ATGAAGAAAAATATAGATTCAAATATTAAAACGATCGTCAGCGCGCTTAAAACGCTGCGTGAACAGAAACCGCTGGTGGT
CAATATTAGTAATTATGTCGCGATGAATAATACCGCTAATGCCTTGCTTGCCCTTGGTGCGTCGCCGATTATGGCGCATT
CCCGTGATGAAATGGCTGAAATGCTGTCTTTTTCTGGTGCTTTGGTAGTCAATATCGGTACTCTGGATAGTCTCTGGATT
CCGCGTATGTTGTTTGCCGTCGAGCAGGCTAATATCCATAAAAAAACGGTAGTGCTTGATCCTGTCGGATGCGGTGCAAG
CAGCCTGCGAACGGCAACCTCATGGCAAATTGCGGAAGCCGCCAACAAGCTAATTATTAGAGGAAATGCATCAGAAATCA
TTGCCTTAGCCGGAGAGAAAGGACAAAGCAAAGGTGTTGATTCCCTTGATAGCAGTGAGGCGGCGCTTGGTGCCGCACGG
TATTTGCGTGACACTTATCAGTGCGCTGTAGTTATTTCTGGGGCGACAGATTTTATTGTCACCGCAGGTGGGCAAATTCA
GCTTAATAACGGTCATGCCATGATGCCTTATGTGACGGCAATGGGCTGCACACTCAGTGCCTTAACTGGAGCTTTTGCAG
CCGTTGGTGAAGAAACTGGATTAGCGGCTGCAGCCGTTTTAGGCGTTGCGGGTGAAATCGCTGCAGAGCAATCAAAAGGC
CCCGGCAGTCTGCAGCTTAATCTTTTAGATGCTTTATATCAGCTCGATGAAGCGACGCTGATTCAGCGTTTAAAATTATC
CTAG

Upstream 100 bases:

>100_bases
AAAACGGTTACTACCGAGGACACAGAGCAAAAGTTTGAGCGTAAAAGGGTCTTTTAAATTAGTTTTGGTAAATTAGAGCA
GCAGCTCAGAAGGATAAAAT

Downstream 100 bases:

>100_bases
CCTGTATCAAAAAATTTGGAGAATAAAGAGTTGGAGAAAAAAGTGAACCCCTATCGTTTATATTTAGTGACCGATGACCA
GCAGAATCTTGAAACATTGA

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MKKNIDSNIKTIVSALKTLREQKPLVVNISNYVAMNNTANALLALGASPIMAHSRDEMAEMLSFSGALVVNIGTLDSLWI
PRMLFAVEQANIHKKTVVLDPVGCGASSLRTATSWQIAEAANKLIIRGNASEIIALAGEKGQSKGVDSLDSSEAALGAAR
YLRDTYQCAVVISGATDFIVTAGGQIQLNNGHAMMPYVTAMGCTLSALTGAFAAVGEETGLAAAAVLGVAGEIAAEQSKG
PGSLQLNLLDALYQLDEATLIQRLKLS

Sequences:

>Translated_267_residues
MKKNIDSNIKTIVSALKTLREQKPLVVNISNYVAMNNTANALLALGASPIMAHSRDEMAEMLSFSGALVVNIGTLDSLWI
PRMLFAVEQANIHKKTVVLDPVGCGASSLRTATSWQIAEAANKLIIRGNASEIIALAGEKGQSKGVDSLDSSEAALGAAR
YLRDTYQCAVVISGATDFIVTAGGQIQLNNGHAMMPYVTAMGCTLSALTGAFAAVGEETGLAAAAVLGVAGEIAAEQSKG
PGSLQLNLLDALYQLDEATLIQRLKLS
>Mature_267_residues
MKKNIDSNIKTIVSALKTLREQKPLVVNISNYVAMNNTANALLALGASPIMAHSRDEMAEMLSFSGALVVNIGTLDSLWI
PRMLFAVEQANIHKKTVVLDPVGCGASSLRTATSWQIAEAANKLIIRGNASEIIALAGEKGQSKGVDSLDSSEAALGAAR
YLRDTYQCAVVISGATDFIVTAGGQIQLNNGHAMMPYVTAMGCTLSALTGAFAAVGEETGLAAAAVLGVAGEIAAEQSKG
PGSLQLNLLDALYQLDEATLIQRLKLS

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family

Homologues:

Organism=Escherichia coli, GI1788421, Length=246, Percent_Identity=36.9918699186992, Blast_Score=127, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6325042, Length=276, Percent_Identity=29.3478260869565, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIM_PSYIN (A1SX95)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_943709.1
- ProteinModelPortal:   A1SX95
- SMR:   A1SX95
- STRING:   A1SX95
- GeneID:   4623707
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_2373
- eggNOG:   COG2145
- HOGENOM:   HBG351126
- OMA:   AIRGNAG
- PhylomeDB:   A1SX95
- ProtClustDB:   PRK09355
- BioCyc:   PING357804:PING_2373-MONOMER
- HAMAP:   MF_00228
- InterPro:   IPR000417
- PANTHER:   PTHR20857:SF14
- PIRSF:   PIRSF000513
- PRINTS:   PR01099

Pfam domain/function: PF02110 HK

EC number: =2.7.1.50

Molecular weight: Translated: 27860; Mature: 27860

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00389 ATPASE_DELTA

Important sites: BINDING 51-51 BINDING 127-127 BINDING 173-173 BINDING 200-200

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNIDSNIKTIVSALKTLREQKPLVVNISNYVAMNNTANALLALGASPIMAHSRDEMAE
CCCCCCHHHHHHHHHHHHHHHCCCEEEEECCEEEECCCCHHHEEECCCCHHHCCHHHHHH
MLSFSGALVVNIGTLDSLWIPRMLFAVEQANIHKKTVVLDPVGCGASSLRTATSWQIAEA
HHHCCCEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHCCCCCHHHHHH
ANKLIIRGNASEIIALAGEKGQSKGVDSLDSSEAALGAARYLRDTYQCAVVISGATDFIV
CCEEEEECCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEEEEECCCCEEE
TAGGQIQLNNGHAMMPYVTAMGCTLSALTGAFAAVGEETGLAAAAVLGVAGEIAAEQSKG
ECCCEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCC
PGSLQLNLLDALYQLDEATLIQRLKLS
CCCEEEHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKNIDSNIKTIVSALKTLREQKPLVVNISNYVAMNNTANALLALGASPIMAHSRDEMAE
CCCCCCHHHHHHHHHHHHHHHCCCEEEEECCEEEECCCCHHHEEECCCCHHHCCHHHHHH
MLSFSGALVVNIGTLDSLWIPRMLFAVEQANIHKKTVVLDPVGCGASSLRTATSWQIAEA
HHHCCCEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHCCCCCHHHHHH
ANKLIIRGNASEIIALAGEKGQSKGVDSLDSSEAALGAARYLRDTYQCAVVISGATDFIV
CCEEEEECCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEEEEECCCCEEE
TAGGQIQLNNGHAMMPYVTAMGCTLSALTGAFAAVGEETGLAAAAVLGVAGEIAAEQSKG
ECCCEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCC
PGSLQLNLLDALYQLDEATLIQRLKLS
CCCEEEHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA