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The map label for this gene is dusC [H]

Identifier: 119945939

GI number: 119945939

Start: 2767511

End: 2768455

Strand: Direct

Name: dusC [H]

Synonym: Ping_2279

Alternate gene names: 119945939

Gene position: 2767511-2768455 (Clockwise)

Preceding gene: 119945931

Following gene: 119945947

Centisome position: 60.7

GC content: 45.82

Gene sequence:

>945_bases
ATGCAAATAACACTTGCCCCGATGGAGGGCGTCATGGATCACCAGATGCGCCAATTGTTAACGGCAATTGGTGGTTATGA
TCTCTGCATTACCGAATTTATTCGCGTTGTTGATATGCAACTCCCGGCAAAAGTATACCGCCGTCATAGCCCGGAACTGT
ATAACGACAGTAAAACTGCCTCCGGCACCCCGGTACGCATTCAATTATTAGGTCAAAACCCGCAATGTTTAGCAGAAAAT
GCGCATCAAGCCATCAGTATCGGCTCCGATGGTATTGATCTTAATTTTGGTTGCCCTGCAAAAACGGTTAATAAAAACAA
AGGGGGCGCCGTGTTATTAAAATCACCGGAAGCCTTATATCAAATTGTGACCGAGGTACGTAAAGCGGTGCCCGTATTTC
AAACGCTCAGCGTCAAAATACGACTGGGTTATGAGGATAAGTCTCTCGCAATTGAAAACGCGCAGGCGATCCAAGCCGCA
GGGGCCGATGAACTTGCGGTGCATGCCCGCACGAAGGCAGAAGGTTACCGCCCGCCCGCCCACTGGCATTGGATAGCTAA
AATTAAAGATGCGATCACTATTCCCGTGACAGCAAATGGTGATATTTTCTCTGTTGATGATGCCAAGCGTTGCATGGAAA
TAACCGGTTGTGAGCGATTAATGATCGGCCGGGGTGCCCTGGCAACACCCAACTTAGCACAAGCAATTAAAGGGATTGAA
TCTCCTAATAGCTGGCCAGAGATTTTGAATATACTGGAAAAATATATGTTTGTTCAAACTCACGGCGATCAGGAAAAATA
TTTACCCAGTCGCATTAAACAATGGTTAATATTTATAAAAGCGCAACATCCGCAGGCTGCTGTTTTCCTTCAGCAGGTGC
GCACTATTAAAACAACCCAAGAAATGTTGGCGGCGATCAATCAAGAGCAGTTAAGGTTTAAATAA

Upstream 100 bases:

>100_bases
TATTTGCTGCTACTTTTTTAAGGCGTCGGGACAGATCTTATTTGTCACATAAATCATCATACTCAGGTATACTGCCGCTT
TTTCAAAGAGAGAGTAGCTG

Downstream 100 bases:

>100_bases
TAAAGACTACTTTGTTAACAAAAAAACCACTTCAGGATTTAATATACTGAAGTGGTTTACTGATTTAATTAAATAGTATT
TCTATTTAATTAAACGACGT

Product: dihydrouridine synthase, DuS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MQITLAPMEGVMDHQMRQLLTAIGGYDLCITEFIRVVDMQLPAKVYRRHSPELYNDSKTASGTPVRIQLLGQNPQCLAEN
AHQAISIGSDGIDLNFGCPAKTVNKNKGGAVLLKSPEALYQIVTEVRKAVPVFQTLSVKIRLGYEDKSLAIENAQAIQAA
GADELAVHARTKAEGYRPPAHWHWIAKIKDAITIPVTANGDIFSVDDAKRCMEITGCERLMIGRGALATPNLAQAIKGIE
SPNSWPEILNILEKYMFVQTHGDQEKYLPSRIKQWLIFIKAQHPQAAVFLQQVRTIKTTQEMLAAINQEQLRFK

Sequences:

>Translated_314_residues
MQITLAPMEGVMDHQMRQLLTAIGGYDLCITEFIRVVDMQLPAKVYRRHSPELYNDSKTASGTPVRIQLLGQNPQCLAEN
AHQAISIGSDGIDLNFGCPAKTVNKNKGGAVLLKSPEALYQIVTEVRKAVPVFQTLSVKIRLGYEDKSLAIENAQAIQAA
GADELAVHARTKAEGYRPPAHWHWIAKIKDAITIPVTANGDIFSVDDAKRCMEITGCERLMIGRGALATPNLAQAIKGIE
SPNSWPEILNILEKYMFVQTHGDQEKYLPSRIKQWLIFIKAQHPQAAVFLQQVRTIKTTQEMLAAINQEQLRFK
>Mature_314_residues
MQITLAPMEGVMDHQMRQLLTAIGGYDLCITEFIRVVDMQLPAKVYRRHSPELYNDSKTASGTPVRIQLLGQNPQCLAEN
AHQAISIGSDGIDLNFGCPAKTVNKNKGGAVLLKSPEALYQIVTEVRKAVPVFQTLSVKIRLGYEDKSLAIENAQAIQAA
GADELAVHARTKAEGYRPPAHWHWIAKIKDAITIPVTANGDIFSVDDAKRCMEITGCERLMIGRGALATPNLAQAIKGIE
SPNSWPEILNILEKYMFVQTHGDQEKYLPSRIKQWLIFIKAQHPQAAVFLQQVRTIKTTQEMLAAINQEQLRFK

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=242, Percent_Identity=30.9917355371901, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI239788483, Length=174, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI40807366, Length=297, Percent_Identity=28.6195286195286, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI239788462, Length=222, Percent_Identity=30.1801801801802, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI8923374, Length=146, Percent_Identity=29.4520547945205, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1788462, Length=307, Percent_Identity=49.8371335504886, Blast_Score=312, Evalue=2e-86,
Organism=Escherichia coli, GI1789660, Length=237, Percent_Identity=32.9113924050633, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI145693211, Length=248, Percent_Identity=25.8064516129032, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25144369, Length=218, Percent_Identity=30.2752293577982, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17543114, Length=200, Percent_Identity=27, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17507177, Length=145, Percent_Identity=33.7931034482759, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323560, Length=248, Percent_Identity=25, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323437, Length=197, Percent_Identity=27.9187817258883, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24580595, Length=204, Percent_Identity=33.8235294117647, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19920448, Length=204, Percent_Identity=33.8235294117647, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19921524, Length=183, Percent_Identity=33.879781420765, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24585320, Length=233, Percent_Identity=31.3304721030043, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI45549423, Length=305, Percent_Identity=25.9016393442623, Blast_Score=78, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 34909; Mature: 34909

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQITLAPMEGVMDHQMRQLLTAIGGYDLCITEFIRVVDMQLPAKVYRRHSPELYNDSKTA
CEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCC
SGTPVRIQLLGQNPQCLAENAHQAISIGSDGIDLNFGCPAKTVNKNKGGAVLLKSPEALY
CCCCEEEEEECCCCHHHHHCCHHEEEECCCCEEEECCCCCCCCCCCCCCEEEEECHHHHH
QIVTEVRKAVPVFQTLSVKIRLGYEDKSLAIENAQAIQAAGADELAVHARTKAEGYRPPA
HHHHHHHHHCCCEEEEEEEEEECCCCCCEECCCHHHHHHCCCCHHEEEEEHHCCCCCCCC
HWHWIAKIKDAITIPVTANGDIFSVDDAKRCMEITGCERLMIGRGALATPNLAQAIKGIE
HHHHHEECCCEEEEEEECCCCEEECCHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHCCC
SPNSWPEILNILEKYMFVQTHGDQEKYLPSRIKQWLIFIKAQHPQAAVFLQQVRTIKTTQ
CCCCCHHHHHHHHHHHEEEECCCCHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHH
EMLAAINQEQLRFK
HHHHHHCHHHHCCC
>Mature Secondary Structure
MQITLAPMEGVMDHQMRQLLTAIGGYDLCITEFIRVVDMQLPAKVYRRHSPELYNDSKTA
CEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCC
SGTPVRIQLLGQNPQCLAENAHQAISIGSDGIDLNFGCPAKTVNKNKGGAVLLKSPEALY
CCCCEEEEEECCCCHHHHHCCHHEEEECCCCEEEECCCCCCCCCCCCCCEEEEECHHHHH
QIVTEVRKAVPVFQTLSVKIRLGYEDKSLAIENAQAIQAAGADELAVHARTKAEGYRPPA
HHHHHHHHHCCCEEEEEEEEEECCCCCCEECCCHHHHHHCCCCHHEEEEEHHCCCCCCCC
HWHWIAKIKDAITIPVTANGDIFSVDDAKRCMEITGCERLMIGRGALATPNLAQAIKGIE
HHHHHEECCCEEEEEEECCCCEEECCHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHCCC
SPNSWPEILNILEKYMFVQTHGDQEKYLPSRIKQWLIFIKAQHPQAAVFLQQVRTIKTTQ
CCCCCHHHHHHHHHHHEEEECCCCHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHH
EMLAAINQEQLRFK
HHHHHHCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]