Click here to switch to the map view.

The map label for this gene is napB [H]

Identifier: 119945833

GI number: 119945833

Start: 2647497

End: 2647961

Strand: Reverse

Name: napB [H]

Synonym: Ping_2173

Alternate gene names: 119945833

Gene position: 2647961-2647497 (Counterclockwise)

Preceding gene: 119945834

Following gene: 119945832

Centisome position: 58.07

GC content: 45.16

Gene sequence:

>465_bases
ATGAAAAAATGTCTGACCGGGATTGTGGCTTTAGGTATCTTTGTTCTTAGTACATCCGTATGGAGTGCAGGGGAGGTAAT
GCAAACCGATAATGGGGGACTTACTTCGCTACGGGGTTACCAGCAAATTTCAGAAGTCAATAAATCAGAAGCGATGAAAC
GCGTACCGAGAGATCATTCAACAGTCGATCGAAACTATGTGCAACAACCCCCTGTTATTCCTCATACTACCCGCGGCTAC
CAGGTTAATCTGAACGCCAATAAATGTTTATCCTGCCACAGTTTTAAAAATGCGGGGGCAATGGGTGCAACAAAAATCAG
TGTCACCCATTTTGAAACACGTGAGGGCACCACTTTATCCGATGTATCACCGCGTCGTTATTTCTGCTTACAATGCCATG
TTACTCAAGCAGATGCTAGACCCTTGGTGGGAAATACTTTCCGACCCGTTGACTCGCTACAGTAA

Upstream 100 bases:

>100_bases
CGGCATTCATTTCCAATTTCCAGAAAAAATCTGTTTTGGTGCCGTAAAATATTTAAATTCTTCGTTGTTATTTAGCATTA
ACAGCAGGATGGAGAAAGTT

Downstream 100 bases:

>100_bases
GTTAGGAGCAAACTACTTATGCTAAATTTATTGAAACGGTTATGGACCATTATGAAATCACCCTCTTCTGCCGCGATGGG
ATTTATCCTGTTGATTGGTT

Product: nitrate reductase periplasmic cytochrome c-type subunit NapB

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MKKCLTGIVALGIFVLSTSVWSAGEVMQTDNGGLTSLRGYQQISEVNKSEAMKRVPRDHSTVDRNYVQQPPVIPHTTRGY
QVNLNANKCLSCHSFKNAGAMGATKISVTHFETREGTTLSDVSPRRYFCLQCHVTQADARPLVGNTFRPVDSLQ

Sequences:

>Translated_154_residues
MKKCLTGIVALGIFVLSTSVWSAGEVMQTDNGGLTSLRGYQQISEVNKSEAMKRVPRDHSTVDRNYVQQPPVIPHTTRGY
QVNLNANKCLSCHSFKNAGAMGATKISVTHFETREGTTLSDVSPRRYFCLQCHVTQADARPLVGNTFRPVDSLQ
>Mature_154_residues
MKKCLTGIVALGIFVLSTSVWSAGEVMQTDNGGLTSLRGYQQISEVNKSEAMKRVPRDHSTVDRNYVQQPPVIPHTTRGY
QVNLNANKCLSCHSFKNAGAMGATKISVTHFETREGTTLSDVSPRRYFCLQCHVTQADARPLVGNTFRPVDSLQ

Specific function: Small subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme NapC protein, thus allowing electron flow between membrane and periplasm.

COG id: COG3043

COG function: function code C; Nitrate reductase cytochrome c-type subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082065, Length=150, Percent_Identity=43.3333333333333, Blast_Score=130, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011031
- InterPro:   IPR005591 [H]

Pfam domain/function: PF03892 NapB [H]

EC number: NA

Molecular weight: Translated: 16973; Mature: 16973

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKCLTGIVALGIFVLSTSVWSAGEVMQTDNGGLTSLRGYQQISEVNKSEAMKRVPRDHS
CHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHCCHHHHHHHCCCCCC
TVDRNYVQQPPVIPHTTRGYQVNLNANKCLSCHSFKNAGAMGATKISVTHFETREGTTLS
HHCHHHCCCCCCCCCCCCCEEEEECHHHEEECCCCCCCCCCCCEEEEEEEEECCCCCCCC
DVSPRRYFCLQCHVTQADARPLVGNTFRPVDSLQ
CCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKCLTGIVALGIFVLSTSVWSAGEVMQTDNGGLTSLRGYQQISEVNKSEAMKRVPRDHS
CHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHCCHHHHHHHCCCCCC
TVDRNYVQQPPVIPHTTRGYQVNLNANKCLSCHSFKNAGAMGATKISVTHFETREGTTLS
HHCHHHCCCCCCCCCCCCCEEEEECHHHEEECCCCCCCCCCCCEEEEEEEEECCCCCCCC
DVSPRRYFCLQCHVTQADARPLVGNTFRPVDSLQ
CCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 8039676 [H]