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The map label for this gene is napA

Identifier: 119945834

GI number: 119945834

Start: 2648073

End: 2650568

Strand: Reverse

Name: napA

Synonym: Ping_2174

Alternate gene names: 119945834

Gene position: 2650568-2648073 (Counterclockwise)

Preceding gene: 119945835

Following gene: 119945833

Centisome position: 58.13

GC content: 44.47

Gene sequence:

>2496_bases
ATGAAATTTACGAGACGTGAATTTATGAAAGCGCAGGCTGCCGCATCAGCCGCTGCTGTTGCTGGTATTGCATTACCAGC
AACAGCGAGTAATTTAATTGCAAGTCAAGATTTAACAAAAATAAAATGGGAAAAAGCGCCCTGCAGATTCTGCGGTACAG
GCTGCAGTGTATTGGTCGGTACACAACATGGCCGGGTGGTTGCAACACAAGGGGATCCTGAATCACCGGTCAATAAAGGA
CTAAATTGCGTAAAGGGTTATTTCCTGTCAAAAATCATGTATGGCAAGGATCGTCTTACTACTCCCTTACTGCGTATGAC
CGAGGGTGTATATGATAAAAATGGCCAGTTTGAACCTGTTTCATGGGATGCTGCTTTTGATATTATGGCTGACAAGTTTA
AAGATGCTTTGAAGAAAAAAGGACCCACAGCGGTGGGGATGTTCGGTTCAGGTCAATGGACTTTATGGGAAGGGTACGCT
GCTTCTAAACTAATGAAAGCGGGTTTCCGTACCAATAATTTAGATCCAAACGCGCGCCATTGTATGGCTTCTGCGGTGGG
TGGTTTTATGCGCACTTTCGGTATTGATGAGCCGATGGGCTGTTATGACGACCTTGAAAATGCCGATGTGTTTGTGCTGT
GGGGATCTAACATGGCGGAGATGCATCCTATTCTTTGGTCGCGTTTAGCCGATCGCCGTTTAAGCAACCCCGATGTCAGT
GTGCACGTGTTGTCTACTTACACACACCGTTCATTTGAATTGGCAGATAATGGCATGATCTTTACGCCACAATCTGATTT
GGCGATCTTAAACTTTATTGCCAACTACATTATTCAAAATGATGCGGTGGATAAAGATTTTATTGAAAAACATACCAATT
TCCGAAAAGGTGTCACGGACATTGGTTATGGTTTACGGCCTACTGATCCTTTACAAAAAGCCGCTGAAAATCCGGATTCT
GGTGCGTCGTCGCCGATAACCTTTGAAGAGTTTGCCGAGTATGTTTCTGAATTTGATGTTGATTATGCCGTTAAAATGTC
AGGTGTCCCTGCTGAAAAATTGATTGAATTGGCCAAAGTCTACGCTGATCCGGCTAAGAAGGTCGTTTCATATTGGACCA
TGGGGTTCAATCAACATACTCGTGGTGTTTGGGCAAATAACTTAATGTATAACGTGCATCTGTTAACCGGTAAAATTTCT
AAACCAGGATCCGGACCCTTCTCATTAACAGGTCAACCCTCAGCTTGCGGTACCGCACGTGAAGTGGGTACATTCTCTCA
CCGTCTACCTGCTGATTTAGTGGTGGCAAATCCTAAACATCGTGCCATTGCCGAAAAATTATGGAAACTGCCGGAAGGTA
CAATAGAACCTAAACCCGGCTATCACGCTGTATTACAAAACCGTATGCTCAAAGACAGTAAGTTAAATGCTTACTGGGTG
ATGTGTAATAACAACGTGCAGGCGGGTGCCAATATTAATGAAGAAATTATTCCAGGTTACCGTAATCCGGAAAATTTTAT
TGTAGTGTCAGACCCTTACCCGACTGTTACTGCGCAAATGGGGGACCTTATTTTACCGACTGCGATGTGGGTGGAAAAAG
AGGGCGCTTATGGCAATGCCGAACGTCGTACTCAATTCTGGCATCAACAAGTTAAAGCACCCGAAGGTGCAAAATCTGAT
TTATGGCAATTAGTTGAATTTTCTAAACGTTTTAAAATTGAAGAAGTTTGGACGGAAGATCTGATCGCAAAAATGCCGGA
AGTGAGGGGGGAAACTTTATTTGATGTGCTTTATAAAAATGGTCAGGTAGATGCTTTTGGTTTAGACGAAGTAGCTGACG
AGCGATTAAATGACGAAGCGCGTGATTTTGGCTTCTACATTCAAAAAGGTTTGTTTGAAGAATACGCTTCATTTGGACGT
GGTCACGGTCATGATTTAGCGCCTTTTGATCAGTATCATGAAGCGCGTGGTCTGCGTTGGCCGGTTGTTAACGGCAAAGA
AACCCTGTGGCGTTTCCGTGAAGGGTACGACCCCTATGTCGAAAAAGGCAGTGAAGTTCAGTTCTATGGCCATCCGGATA
AGAAAGCGGTTATTTTCGCACTGCCCTATGAGCCACCCGCTGAATCACCCGATCAAGAGTACGACCTGTGGTTAAGCACC
GGTCGTGTATTAGAACATTGGCATTCAGGATCCATGACCCGTCGTGTACCCGAACTTTATAAAGCATTTCCTGATGCAGT
GGTGTTTATGCACCCAGATGATGCTACAGCACGGGGTGTCCGACGTGGTGATGAGATTGTACTGGCTTCACGTCGTGGTG
AGTTAACTTCTCGTGTTGAAACCCGCGGCCGAAATCGGCCCCCTCGAGGTTTAGTCTTTATGCCCTGGTTTGATGCGAAG
CAGTTAGTGAATAAAATTACACTTGATGCAACGGATCCGCTGTCGAAACAAACGGATTACAAAAAATGTGCAGTGAAAAT
TACTAAGAAAGTTTAA

Upstream 100 bases:

>100_bases
GTCTATCATGAATTTGAAACATTAGAATCTGAGTCTTAATTGATTAATCAGTGGAAACTCAATTAAATCATTTGGTTTCA
TCAAATAGTGGGGAAGCAAC

Downstream 100 bases:

>100_bases
TTCGCGCGTTACGGCATTCATTTCCAATTTCCAGAAAAAATCTGTTTTGGTGCCGTAAAATATTTAAATTCTTCGTTGTT
ATTTAGCATTAACAGCAGGA

Product: nitrate reductase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 831; Mature: 831

Protein sequence:

>831_residues
MKFTRREFMKAQAAASAAAVAGIALPATASNLIASQDLTKIKWEKAPCRFCGTGCSVLVGTQHGRVVATQGDPESPVNKG
LNCVKGYFLSKIMYGKDRLTTPLLRMTEGVYDKNGQFEPVSWDAAFDIMADKFKDALKKKGPTAVGMFGSGQWTLWEGYA
ASKLMKAGFRTNNLDPNARHCMASAVGGFMRTFGIDEPMGCYDDLENADVFVLWGSNMAEMHPILWSRLADRRLSNPDVS
VHVLSTYTHRSFELADNGMIFTPQSDLAILNFIANYIIQNDAVDKDFIEKHTNFRKGVTDIGYGLRPTDPLQKAAENPDS
GASSPITFEEFAEYVSEFDVDYAVKMSGVPAEKLIELAKVYADPAKKVVSYWTMGFNQHTRGVWANNLMYNVHLLTGKIS
KPGSGPFSLTGQPSACGTAREVGTFSHRLPADLVVANPKHRAIAEKLWKLPEGTIEPKPGYHAVLQNRMLKDSKLNAYWV
MCNNNVQAGANINEEIIPGYRNPENFIVVSDPYPTVTAQMGDLILPTAMWVEKEGAYGNAERRTQFWHQQVKAPEGAKSD
LWQLVEFSKRFKIEEVWTEDLIAKMPEVRGETLFDVLYKNGQVDAFGLDEVADERLNDEARDFGFYIQKGLFEEYASFGR
GHGHDLAPFDQYHEARGLRWPVVNGKETLWRFREGYDPYVEKGSEVQFYGHPDKKAVIFALPYEPPAESPDQEYDLWLST
GRVLEHWHSGSMTRRVPELYKAFPDAVVFMHPDDATARGVRRGDEIVLASRRGELTSRVETRGRNRPPRGLVFMPWFDAK
QLVNKITLDATDPLSKQTDYKKCAVKITKKV

Sequences:

>Translated_831_residues
MKFTRREFMKAQAAASAAAVAGIALPATASNLIASQDLTKIKWEKAPCRFCGTGCSVLVGTQHGRVVATQGDPESPVNKG
LNCVKGYFLSKIMYGKDRLTTPLLRMTEGVYDKNGQFEPVSWDAAFDIMADKFKDALKKKGPTAVGMFGSGQWTLWEGYA
ASKLMKAGFRTNNLDPNARHCMASAVGGFMRTFGIDEPMGCYDDLENADVFVLWGSNMAEMHPILWSRLADRRLSNPDVS
VHVLSTYTHRSFELADNGMIFTPQSDLAILNFIANYIIQNDAVDKDFIEKHTNFRKGVTDIGYGLRPTDPLQKAAENPDS
GASSPITFEEFAEYVSEFDVDYAVKMSGVPAEKLIELAKVYADPAKKVVSYWTMGFNQHTRGVWANNLMYNVHLLTGKIS
KPGSGPFSLTGQPSACGTAREVGTFSHRLPADLVVANPKHRAIAEKLWKLPEGTIEPKPGYHAVLQNRMLKDSKLNAYWV
MCNNNVQAGANINEEIIPGYRNPENFIVVSDPYPTVTAQMGDLILPTAMWVEKEGAYGNAERRTQFWHQQVKAPEGAKSD
LWQLVEFSKRFKIEEVWTEDLIAKMPEVRGETLFDVLYKNGQVDAFGLDEVADERLNDEARDFGFYIQKGLFEEYASFGR
GHGHDLAPFDQYHEARGLRWPVVNGKETLWRFREGYDPYVEKGSEVQFYGHPDKKAVIFALPYEPPAESPDQEYDLWLST
GRVLEHWHSGSMTRRVPELYKAFPDAVVFMHPDDATARGVRRGDEIVLASRRGELTSRVETRGRNRPPRGLVFMPWFDAK
QLVNKITLDATDPLSKQTDYKKCAVKITKKV
>Mature_831_residues
MKFTRREFMKAQAAASAAAVAGIALPATASNLIASQDLTKIKWEKAPCRFCGTGCSVLVGTQHGRVVATQGDPESPVNKG
LNCVKGYFLSKIMYGKDRLTTPLLRMTEGVYDKNGQFEPVSWDAAFDIMADKFKDALKKKGPTAVGMFGSGQWTLWEGYA
ASKLMKAGFRTNNLDPNARHCMASAVGGFMRTFGIDEPMGCYDDLENADVFVLWGSNMAEMHPILWSRLADRRLSNPDVS
VHVLSTYTHRSFELADNGMIFTPQSDLAILNFIANYIIQNDAVDKDFIEKHTNFRKGVTDIGYGLRPTDPLQKAAENPDS
GASSPITFEEFAEYVSEFDVDYAVKMSGVPAEKLIELAKVYADPAKKVVSYWTMGFNQHTRGVWANNLMYNVHLLTGKIS
KPGSGPFSLTGQPSACGTAREVGTFSHRLPADLVVANPKHRAIAEKLWKLPEGTIEPKPGYHAVLQNRMLKDSKLNAYWV
MCNNNVQAGANINEEIIPGYRNPENFIVVSDPYPTVTAQMGDLILPTAMWVEKEGAYGNAERRTQFWHQQVKAPEGAKSD
LWQLVEFSKRFKIEEVWTEDLIAKMPEVRGETLFDVLYKNGQVDAFGLDEVADERLNDEARDFGFYIQKGLFEEYASFGR
GHGHDLAPFDQYHEARGLRWPVVNGKETLWRFREGYDPYVEKGSEVQFYGHPDKKAVIFALPYEPPAESPDQEYDLWLST
GRVLEHWHSGSMTRRVPELYKAFPDAVVFMHPDDATARGVRRGDEIVLASRRGELTSRVETRGRNRPPRGLVFMPWFDAK
QLVNKITLDATDPLSKQTDYKKCAVKITKKV

Specific function: Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and peripla

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily

Homologues:

Organism=Escherichia coli, GI1788534, Length=829, Percent_Identity=72.8588661037394, Blast_Score=1309, Evalue=0.0,
Organism=Escherichia coli, GI3868721, Length=794, Percent_Identity=23.8035264483627, Blast_Score=204, Evalue=2e-53,
Organism=Escherichia coli, GI3868720, Length=660, Percent_Identity=23.6363636363636, Blast_Score=127, Evalue=4e-30,
Organism=Escherichia coli, GI3868719, Length=506, Percent_Identity=23.9130434782609, Blast_Score=113, Evalue=5e-26,
Organism=Escherichia coli, GI87081797, Length=881, Percent_Identity=22.5879682179342, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1787870, Length=887, Percent_Identity=23.5625704622322, Blast_Score=103, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NAPA_PSYIN (A1SWQ0)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_943514.1
- ProteinModelPortal:   A1SWQ0
- SMR:   A1SWQ0
- STRING:   A1SWQ0
- GeneID:   4625329
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_2174
- eggNOG:   COG0243
- HOGENOM:   HBG715033
- OMA:   NMLYNIH
- PhylomeDB:   A1SWQ0
- ProtClustDB:   PRK13532
- BioCyc:   PING357804:PING_2174-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01630
- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR010051
- InterPro:   IPR006311
- Gene3D:   G3DSA:2.40.40.20
- SMART:   SM00926
- TIGRFAMs:   TIGR01706
- TIGRFAMs:   TIGR01409

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding; SSF50692 Asp_decarb_fold

EC number: =1.7.99.4

Molecular weight: Translated: 93214; Mature: 93214

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1; PS00490 MOLYBDOPTERIN_PROK_2; PS00932 MOLYBDOPTERIN_PROK_3; PS51318 TAT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTRREFMKAQAAASAAAVAGIALPATASNLIASQDLTKIKWEKAPCRFCGTGCSVLVG
CCCHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHCCCHHEEECCCCCCCCCCCCEEEEE
TQHGRVVATQGDPESPVNKGLNCVKGYFLSKIMYGKDRLTTPLLRMTEGVYDKNGQFEPV
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCC
SWDAAFDIMADKFKDALKKKGPTAVGMFGSGQWTLWEGYAASKLMKAGFRTNNLDPNARH
CCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECCHHHHHHHHCCCCCCCCCCHHHH
CMASAVGGFMRTFGIDEPMGCYDDLENADVFVLWGSNMAEMHPILWSRLADRRLSNPDVS
HHHHHHHHHHHHHCCCCCCCCHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCEE
VHVLSTYTHRSFELADNGMIFTPQSDLAILNFIANYIIQNDAVDKDFIEKHTNFRKGVTD
EEEEEECCCCEEEECCCCEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHH
IGYGLRPTDPLQKAAENPDSGASSPITFEEFAEYVSEFDVDYAVKMSGVPAEKLIELAKV
HCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHH
YADPAKKVVSYWTMGFNQHTRGVWANNLMYNVHLLTGKISKPGSGPFSLTGQPSACGTAR
HHHHHHHHHHHHHCCCCCCCCCEEECCEEEEEEEEEECCCCCCCCCEEECCCCCCCCCHH
EVGTFSHRLPADLVVANPKHRAIAEKLWKLPEGTIEPKPGYHAVLQNRMLKDSKLNAYWV
HHCCHHHCCCCCEEEECCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEE
MCNNNVQAGANINEEIIPGYRNPENFIVVSDPYPTVTAQMGDLILPTAMWVEKEGAYGNA
EECCCCCCCCCCCHHHCCCCCCCCCEEEECCCCCCHHHHHCCCCCCHHHEEECCCCCCCH
ERRTQFWHQQVKAPEGAKSDLWQLVEFSKRFKIEEVWTEDLIAKMPEVRGETLFDVLYKN
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHCC
GQVDAFGLDEVADERLNDEARDFGFYIQKGLFEEYASFGRGHGHDLAPFDQYHEARGLRW
CCEEECCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCC
PVVNGKETLWRFREGYDPYVEKGSEVQFYGHPDKKAVIFALPYEPPAESPDQEYDLWLST
CCCCCHHHHHHHHCCCCHHHCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCHHHHHHH
GRVLEHWHSGSMTRRVPELYKAFPDAVVFMHPDDATARGVRRGDEIVLASRRGELTSRVE
HHHHHHHCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHCCCCCEEEEEECCCCHHHHHH
TRGRNRPPRGLVFMPWFDAKQLVNKITLDATDPLSKQTDYKKCAVKITKKV
HCCCCCCCCCEEEECCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MKFTRREFMKAQAAASAAAVAGIALPATASNLIASQDLTKIKWEKAPCRFCGTGCSVLVG
CCCHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHCCCHHEEECCCCCCCCCCCCEEEEE
TQHGRVVATQGDPESPVNKGLNCVKGYFLSKIMYGKDRLTTPLLRMTEGVYDKNGQFEPV
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCC
SWDAAFDIMADKFKDALKKKGPTAVGMFGSGQWTLWEGYAASKLMKAGFRTNNLDPNARH
CCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECCHHHHHHHHCCCCCCCCCCHHHH
CMASAVGGFMRTFGIDEPMGCYDDLENADVFVLWGSNMAEMHPILWSRLADRRLSNPDVS
HHHHHHHHHHHHHCCCCCCCCHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCEE
VHVLSTYTHRSFELADNGMIFTPQSDLAILNFIANYIIQNDAVDKDFIEKHTNFRKGVTD
EEEEEECCCCEEEECCCCEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHH
IGYGLRPTDPLQKAAENPDSGASSPITFEEFAEYVSEFDVDYAVKMSGVPAEKLIELAKV
HCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHH
YADPAKKVVSYWTMGFNQHTRGVWANNLMYNVHLLTGKISKPGSGPFSLTGQPSACGTAR
HHHHHHHHHHHHHCCCCCCCCCEEECCEEEEEEEEEECCCCCCCCCEEECCCCCCCCCHH
EVGTFSHRLPADLVVANPKHRAIAEKLWKLPEGTIEPKPGYHAVLQNRMLKDSKLNAYWV
HHCCHHHCCCCCEEEECCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEE
MCNNNVQAGANINEEIIPGYRNPENFIVVSDPYPTVTAQMGDLILPTAMWVEKEGAYGNA
EECCCCCCCCCCCHHHCCCCCCCCCEEEECCCCCCHHHHHCCCCCCHHHEEECCCCCCCH
ERRTQFWHQQVKAPEGAKSDLWQLVEFSKRFKIEEVWTEDLIAKMPEVRGETLFDVLYKN
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHCC
GQVDAFGLDEVADERLNDEARDFGFYIQKGLFEEYASFGRGHGHDLAPFDQYHEARGLRW
CCEEECCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCC
PVVNGKETLWRFREGYDPYVEKGSEVQFYGHPDKKAVIFALPYEPPAESPDQEYDLWLST
CCCCCHHHHHHHHCCCCHHHCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCHHHHHHH
GRVLEHWHSGSMTRRVPELYKAFPDAVVFMHPDDATARGVRRGDEIVLASRRGELTSRVE
HHHHHHHCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHCCCCCEEEEEECCCCHHHHHH
TRGRNRPPRGLVFMPWFDAKQLVNKITLDATDPLSKQTDYKKCAVKITKKV
HCCCCCCCCCEEEECCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA