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The map label for this gene is fumB [H]

Identifier: 119945664

GI number: 119945664

Start: 2405550

End: 2407088

Strand: Reverse

Name: fumB [H]

Synonym: Ping_1977

Alternate gene names: 119945664

Gene position: 2407088-2405550 (Counterclockwise)

Preceding gene: 119945666

Following gene: 119945663

Centisome position: 52.79

GC content: 43.47

Gene sequence:

>1539_bases
ATGACTATTATTAAGCAACAGGATTTTGTCAACAGCGTTGCGAATGCATTACAGTTTATCTCATACTATCACCCGATTGA
TTTTATTAAAGCAATGCATAAGGCTTATCTGGCGGAGCAAAATCCCGCAGCAAAAGATGCGATTGCTCAAATTCTAATTA
ATTCTCGTATGTCGGCCGAAGGCAAACGTCCTATTTGCCAAGATACGGGCATTGTCACTGTTTTTGTTAAAATCGGTATG
AAGGTTCAATTTGAAGGTGAACTGACAGTACAAGAGTTAGTCGATGCCGGCGTCCGCAAGGCTTATCTGGATCCGAGTAA
TCCTCTGCGTGCTTCAATTGTTGCAGACCCCGCTGGCGCACGCAAAAATACACAGGATAATACTCCCTCAGTGGTTCATG
TTGAAATGGTAACGGGGAATAAAGTTGAAGTCATGATCGCGGCCAAAGGCGGTGGCTCAGAAAATAAATCAAAAATGGTG
ATGTTAAATCCGTCGGATTCAATTGCTGACTGGGTAGAAAAAACCGTGCCAAGCATGGGGGCAGGTTGGTGTCCTCCGGG
TATGTTGGGTATCGGCATAGGGGGAACGACAGAAAAAGCGGCGGTAATGGCTAAAGAGTCATTAATGGAAGCGGTGGATA
TCTTTGATTTACAAGAAAAAGCTAAAAACAATCCTGAGCAGTTAAATGCAGATGAAAAACTCCGTTTAGAAATTATGCAG
CGTGTTAATGCACTCGGTATTGGTGCACAGGGTTTAGGTGGTTTAACCACTGTACTTGATATTAAAATTAAAAGTTGTCC
AACACATGCGGCATCGTTGCCTGTGGTTATGATCCCAAATTGCGCCGCAACAAGACATGCGCATTTTGAACTTGATGGTA
GCGGGGAAGCTGAATTAGTCGCGCCTAAATTAAGTGAGTGGCCGGAAATTACCCGGGAAGGAGGTGAAAATGTTCATCGT
GTGAATGTTAATAATCTCACTAAAGCCGATATGGCTTCCTGGAAAAGTGGCGATTCTCTTCTTCTGTCAGGAAAAATATT
AACCGGCCGGGATGCGGCTCACAAACGTATTCACTCTCTAATAACTTCAGGAAAGGGATTACCAGAAGGAGTTGATTTTA
ATGGGCGATTTATTTATTACGTTGGCCCGGTTGATGCAGTTCGAGACGAAGTAGTTGGCCCTGCGGGACCAACGACAGCC
ACAAGGATGGATAAATATACAGACTTTATGCTCGAAGAAACCGGGTTACTCGGTATGATCGGAAAATCTGAACGTGGGCA
ACAAACCGTTGATTCAATCGCCAAACATAAGTCTGTCTATTTAATGGCCGTTGGCGGGGCTGCTTATTTAGTTTCTAAGG
CGATAAAAAAATCCCGAGTGGTCGCTTTTGCTGATTTAGGCATGGAAGCAATTTATGAATTTGATGTTGAAGATATGCCT
GTTACCGTGGCGGTTGATAGTGAAGGTAATAATGTACATGAGCAGGGCCCTGCCATCTGGAAAGTTAAAATAGAAGAGAT
TGCTGATAAATTAGCGTAA

Upstream 100 bases:

>100_bases
TTTTGTAACAAATGTTTTGGTGGATATGTATTTACAGATTAACATTCTCTGTACTATATATCAGCCCTTTATACCCTCAT
TTATATTAACGGAGTTTTAC

Downstream 100 bases:

>100_bases
TCATTACGTTTTTTATTGCTTGAAAAGGCAGTAATGGTATAACTCAGTACAGGAATATAACGTCAGTATAGGTAAGGAAG
AATTATGTTTGAACAAGTGA

Product: fumarate hydratase

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MTIIKQQDFVNSVANALQFISYYHPIDFIKAMHKAYLAEQNPAAKDAIAQILINSRMSAEGKRPICQDTGIVTVFVKIGM
KVQFEGELTVQELVDAGVRKAYLDPSNPLRASIVADPAGARKNTQDNTPSVVHVEMVTGNKVEVMIAAKGGGSENKSKMV
MLNPSDSIADWVEKTVPSMGAGWCPPGMLGIGIGGTTEKAAVMAKESLMEAVDIFDLQEKAKNNPEQLNADEKLRLEIMQ
RVNALGIGAQGLGGLTTVLDIKIKSCPTHAASLPVVMIPNCAATRHAHFELDGSGEAELVAPKLSEWPEITREGGENVHR
VNVNNLTKADMASWKSGDSLLLSGKILTGRDAAHKRIHSLITSGKGLPEGVDFNGRFIYYVGPVDAVRDEVVGPAGPTTA
TRMDKYTDFMLEETGLLGMIGKSERGQQTVDSIAKHKSVYLMAVGGAAYLVSKAIKKSRVVAFADLGMEAIYEFDVEDMP
VTVAVDSEGNNVHEQGPAIWKVKIEEIADKLA

Sequences:

>Translated_512_residues
MTIIKQQDFVNSVANALQFISYYHPIDFIKAMHKAYLAEQNPAAKDAIAQILINSRMSAEGKRPICQDTGIVTVFVKIGM
KVQFEGELTVQELVDAGVRKAYLDPSNPLRASIVADPAGARKNTQDNTPSVVHVEMVTGNKVEVMIAAKGGGSENKSKMV
MLNPSDSIADWVEKTVPSMGAGWCPPGMLGIGIGGTTEKAAVMAKESLMEAVDIFDLQEKAKNNPEQLNADEKLRLEIMQ
RVNALGIGAQGLGGLTTVLDIKIKSCPTHAASLPVVMIPNCAATRHAHFELDGSGEAELVAPKLSEWPEITREGGENVHR
VNVNNLTKADMASWKSGDSLLLSGKILTGRDAAHKRIHSLITSGKGLPEGVDFNGRFIYYVGPVDAVRDEVVGPAGPTTA
TRMDKYTDFMLEETGLLGMIGKSERGQQTVDSIAKHKSVYLMAVGGAAYLVSKAIKKSRVVAFADLGMEAIYEFDVEDMP
VTVAVDSEGNNVHEQGPAIWKVKIEEIADKLA
>Mature_511_residues
TIIKQQDFVNSVANALQFISYYHPIDFIKAMHKAYLAEQNPAAKDAIAQILINSRMSAEGKRPICQDTGIVTVFVKIGMK
VQFEGELTVQELVDAGVRKAYLDPSNPLRASIVADPAGARKNTQDNTPSVVHVEMVTGNKVEVMIAAKGGGSENKSKMVM
LNPSDSIADWVEKTVPSMGAGWCPPGMLGIGIGGTTEKAAVMAKESLMEAVDIFDLQEKAKNNPEQLNADEKLRLEIMQR
VNALGIGAQGLGGLTTVLDIKIKSCPTHAASLPVVMIPNCAATRHAHFELDGSGEAELVAPKLSEWPEITREGGENVHRV
NVNNLTKADMASWKSGDSLLLSGKILTGRDAAHKRIHSLITSGKGLPEGVDFNGRFIYYVGPVDAVRDEVVGPAGPTTAT
RMDKYTDFMLEETGLLGMIGKSERGQQTVDSIAKHKSVYLMAVGGAAYLVSKAIKKSRVVAFADLGMEAIYEFDVEDMPV
TVAVDSEGNNVHEQGPAIWKVKIEEIADKLA

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=459, Percent_Identity=32.8976034858388, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI1787897, Length=515, Percent_Identity=31.4563106796116, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1789442, Length=278, Percent_Identity=27.6978417266187, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1789443, Length=188, Percent_Identity=27.6595744680851, Blast_Score=86, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 55111; Mature: 54980

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIIKQQDFVNSVANALQFISYYHPIDFIKAMHKAYLAEQNPAAKDAIAQILINSRMSAE
CCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
GKRPICQDTGIVTVFVKIGMKVQFEGELTVQELVDAGVRKAYLDPSNPLRASIVADPAGA
CCCCCCCCCCEEEEEEEECEEEEECCCCCHHHHHHHHHHEEECCCCCCCEEEEEECCCCC
RKNTQDNTPSVVHVEMVTGNKVEVMIAAKGGGSENKSKMVMLNPSDSIADWVEKTVPSMG
CCCCCCCCCCEEEEEEECCCEEEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHCCCCC
AGWCPPGMLGIGIGGTTEKAAVMAKESLMEAVDIFDLQEKAKNNPEQLNADEKLRLEIMQ
CCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHH
RVNALGIGAQGLGGLTTVLDIKIKSCPTHAASLPVVMIPNCAATRHAHFELDGSGEAELV
HHHHHCCCCCCCCCEEEEEEEEEECCCCCCCCCCEEECCCCCCCCCEEEEECCCCCCEEE
APKLSEWPEITREGGENVHRVNVNNLTKADMASWKSGDSLLLSGKILTGRDAAHKRIHSL
CCCHHCCHHHHHCCCCCEEEEECCCCCHHHHHCCCCCCEEEEECEEEECCHHHHHHHHHH
ITSGKGLPEGVDFNGRFIYYVGPVDAVRDEVVGPAGPTTATRMDKYTDFMLEETGLLGMI
HHCCCCCCCCCCCCCEEEEEECCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEC
GKSERGQQTVDSIAKHKSVYLMAVGGAAYLVSKAIKKSRVVAFADLGMEAIYEFDVEDMP
CCCCCCHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHCEEEEECCCHHHHEECCCCCCC
VTVAVDSEGNNVHEQGPAIWKVKIEEIADKLA
EEEEECCCCCCCHHCCCEEEEEEHHHHHHHCC
>Mature Secondary Structure 
TIIKQQDFVNSVANALQFISYYHPIDFIKAMHKAYLAEQNPAAKDAIAQILINSRMSAE
CCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
GKRPICQDTGIVTVFVKIGMKVQFEGELTVQELVDAGVRKAYLDPSNPLRASIVADPAGA
CCCCCCCCCCEEEEEEEECEEEEECCCCCHHHHHHHHHHEEECCCCCCCEEEEEECCCCC
RKNTQDNTPSVVHVEMVTGNKVEVMIAAKGGGSENKSKMVMLNPSDSIADWVEKTVPSMG
CCCCCCCCCCEEEEEEECCCEEEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHCCCCC
AGWCPPGMLGIGIGGTTEKAAVMAKESLMEAVDIFDLQEKAKNNPEQLNADEKLRLEIMQ
CCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHH
RVNALGIGAQGLGGLTTVLDIKIKSCPTHAASLPVVMIPNCAATRHAHFELDGSGEAELV
HHHHHCCCCCCCCCEEEEEEEEEECCCCCCCCCCEEECCCCCCCCCEEEEECCCCCCEEE
APKLSEWPEITREGGENVHRVNVNNLTKADMASWKSGDSLLLSGKILTGRDAAHKRIHSL
CCCHHCCHHHHHCCCCCEEEEECCCCCHHHHHCCCCCCEEEEECEEEECCHHHHHHHHHH
ITSGKGLPEGVDFNGRFIYYVGPVDAVRDEVVGPAGPTTATRMDKYTDFMLEETGLLGMI
HHCCCCCCCCCCCCCEEEEEECCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEC
GKSERGQQTVDSIAKHKSVYLMAVGGAAYLVSKAIKKSRVVAFADLGMEAIYEFDVEDMP
CCCCCCHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHCEEEEECCCHHHHEECCCCCCC
VTVAVDSEGNNVHEQGPAIWKVKIEEIADKLA
EEEEECCCCCCCHHCCCEEEEEEHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]