Click here to switch to the map view.

The map label for this gene is cysB [H]

Identifier: 119945666

GI number: 119945666

Start: 2408696

End: 2409667

Strand: Reverse

Name: cysB [H]

Synonym: Ping_1979

Alternate gene names: 119945666

Gene position: 2409667-2408696 (Counterclockwise)

Preceding gene: 119945672

Following gene: 119945664

Centisome position: 52.85

GC content: 40.23

Gene sequence:

>972_bases
ATGAAATTACAACAATTACGTTATATCGTCGAAGTTTTAAATAATAATCTAAATGTATCTGCAACGGCTGAGAATTTGTA
CACGTCGCAGCCGGGGATAAGTAAGCAAGTTCGCATGTTGGAAGATGAATTGGGGATTCAAGTTTTTGAACGAAGCGGAA
AACATTTAACTAAGGTAACCCCTGCAGGGAAAGAGGTTATTCGTTATGCTTCTGAGATACTGGGTAAAGTTGAAAGTATC
AAAGCCATTGCTTGCCAATATACTCAGCCTGATCAGGGAACGTTAAATATTGCCACAACCCATACTCAGGCGCGTTATGC
TTTACCGGATGTTATTCAAGGGTTTATAAAACGCTATCCAAAAGTGTCGCTGCATATGCATCAGGGGACCCCGACGCAAA
TAAGCGAGTCAGTCGCCAAAGGGAAAACCGATTTTGCAATTGCAACTGAAGCACTGCATCTCTATCAAGATTTGATTATG
TTACCTTGTTACCATTGGAATCGTTCGGTGATTGTGCCTAAAGAGCATCCTCTGGCGAATATTAAAGAGATTAGCATTGC
AGATATTGCCAAGTACCCGATTGTGACTTATGTTTTTGGTTTTACCGGACGTTCGGAGTTAGATGAAGCATTTAACTCTG
CGGGATTTAAACCTAATGTTGTCTTTACTGCAACCGATGCTGATGTGATAAAAACCTATGTGCGTTTAGGATTGGGTGTT
GGGGTTGTTGCCAGTATGGCCATTAATGAAGATGATGACGATTTTTGCGTGATTGATGCGCGTCATATTTTCAAATCAAG
CACCACCAGTATTGGTTTCAGAAAGGGCTCTTTTTTACGTGATTATATGTATGATTTTATGGTTCGATTTGCACCCCATT
TGAATCGTCCACTGGTAGAAAAAGCGATTCAGTTAAAATATAACGCTGAAGTCAGTCAATTATTTAAAGATATTGAACTG
CCTGTGCGCTGA

Upstream 100 bases:

>100_bases
ATAACTAATTATATATATTTGATTTAACTCTGTTAGAATCAAATGGAATATCAAATGGAATATCAAATTAAAAAATCTTT
CTATAACTAATGGCTTTTAC

Downstream 100 bases:

>100_bases
TTAAAGCTCGAAGCTCGAAGCTCGAAGCTCGAAGCCCGAAAGCCCGAAGGCTCGAAGCTTTATTTGTTCAAAATTTCCAG
ACAGGGTAAAATTTTACTTA

Product: transcriptional regulator CysB

Products: NA

Alternate protein names: Cys regulon transcriptional activator [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MKLQQLRYIVEVLNNNLNVSATAENLYTSQPGISKQVRMLEDELGIQVFERSGKHLTKVTPAGKEVIRYASEILGKVESI
KAIACQYTQPDQGTLNIATTHTQARYALPDVIQGFIKRYPKVSLHMHQGTPTQISESVAKGKTDFAIATEALHLYQDLIM
LPCYHWNRSVIVPKEHPLANIKEISIADIAKYPIVTYVFGFTGRSELDEAFNSAGFKPNVVFTATDADVIKTYVRLGLGV
GVVASMAINEDDDDFCVIDARHIFKSSTTSIGFRKGSFLRDYMYDFMVRFAPHLNRPLVEKAIQLKYNAEVSQLFKDIEL
PVR

Sequences:

>Translated_323_residues
MKLQQLRYIVEVLNNNLNVSATAENLYTSQPGISKQVRMLEDELGIQVFERSGKHLTKVTPAGKEVIRYASEILGKVESI
KAIACQYTQPDQGTLNIATTHTQARYALPDVIQGFIKRYPKVSLHMHQGTPTQISESVAKGKTDFAIATEALHLYQDLIM
LPCYHWNRSVIVPKEHPLANIKEISIADIAKYPIVTYVFGFTGRSELDEAFNSAGFKPNVVFTATDADVIKTYVRLGLGV
GVVASMAINEDDDDFCVIDARHIFKSSTTSIGFRKGSFLRDYMYDFMVRFAPHLNRPLVEKAIQLKYNAEVSQLFKDIEL
PVR
>Mature_323_residues
MKLQQLRYIVEVLNNNLNVSATAENLYTSQPGISKQVRMLEDELGIQVFERSGKHLTKVTPAGKEVIRYASEILGKVESI
KAIACQYTQPDQGTLNIATTHTQARYALPDVIQGFIKRYPKVSLHMHQGTPTQISESVAKGKTDFAIATEALHLYQDLIM
LPCYHWNRSVIVPKEHPLANIKEISIADIAKYPIVTYVFGFTGRSELDEAFNSAGFKPNVVFTATDADVIKTYVRLGLGV
GVVASMAINEDDDDFCVIDARHIFKSSTTSIGFRKGSFLRDYMYDFMVRFAPHLNRPLVEKAIQLKYNAEVSQLFKDIEL
PVR

Specific function: This protein is a positive regulator of gene expression for the cysteine regulon. The inducer for cysB is N-acetylserine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787530, Length=322, Percent_Identity=70.1863354037267, Blast_Score=493, Evalue=1e-141,
Organism=Escherichia coli, GI1788296, Length=288, Percent_Identity=40.9722222222222, Blast_Score=252, Evalue=3e-68,
Organism=Escherichia coli, GI1790399, Length=285, Percent_Identity=26.3157894736842, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI157672245, Length=252, Percent_Identity=26.5873015873016, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788887, Length=296, Percent_Identity=27.7027027027027, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787879, Length=258, Percent_Identity=25.968992248062, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1789204, Length=226, Percent_Identity=25.2212389380531, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790262, Length=215, Percent_Identity=25.5813953488372, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1788481, Length=250, Percent_Identity=25.2, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787601, Length=149, Percent_Identity=29.5302013422819, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI2367136, Length=242, Percent_Identity=22.3140495867769, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787806, Length=288, Percent_Identity=20.4861111111111, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI145693105, Length=234, Percent_Identity=20.9401709401709, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36294; Mature: 36294

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQQLRYIVEVLNNNLNVSATAENLYTSQPGISKQVRMLEDELGIQVFERSGKHLTKVT
CCHHHHHHHHHHHCCCCCEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEC
PAGKEVIRYASEILGKVESIKAIACQYTQPDQGTLNIATTHTQARYALPDVIQGFIKRYP
CCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEEECCCHHHHCCHHHHHHHHHHCC
KVSLHMHQGTPTQISESVAKGKTDFAIATEALHLYQDLIMLPCYHWNRSVIVPKEHPLAN
CEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEECCCEEECCCCCCCCC
IKEISIADIAKYPIVTYVFGFTGRSELDEAFNSAGFKPNVVFTATDADVIKTYVRLGLGV
CHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHCCH
GVVASMAINEDDDDFCVIDARHIFKSSTTSIGFRKGSFLRDYMYDFMVRFAPHLNRPLVE
HHHHHHHCCCCCCCEEEEEHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHH
KAIQLKYNAEVSQLFKDIELPVR
HHHHHHCCCHHHHHHHHCCCCCC
>Mature Secondary Structure
MKLQQLRYIVEVLNNNLNVSATAENLYTSQPGISKQVRMLEDELGIQVFERSGKHLTKVT
CCHHHHHHHHHHHCCCCCEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEC
PAGKEVIRYASEILGKVESIKAIACQYTQPDQGTLNIATTHTQARYALPDVIQGFIKRYP
CCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEEECCCHHHHCCHHHHHHHHHHCC
KVSLHMHQGTPTQISESVAKGKTDFAIATEALHLYQDLIMLPCYHWNRSVIVPKEHPLAN
CEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEECCCEEECCCCCCCCC
IKEISIADIAKYPIVTYVFGFTGRSELDEAFNSAGFKPNVVFTATDADVIKTYVRLGLGV
CHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHCCH
GVVASMAINEDDDDFCVIDARHIFKSSTTSIGFRKGSFLRDYMYDFMVRFAPHLNRPLVE
HHHHHHHCCCCCCCEEEEEHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHH
KAIQLKYNAEVSQLFKDIELPVR
HHHHHHCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]