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The map label for this gene is hemK [H]

Identifier: 119945324

GI number: 119945324

Start: 1962963

End: 1963802

Strand: Direct

Name: hemK [H]

Synonym: Ping_1608

Alternate gene names: 119945324

Gene position: 1962963-1963802 (Clockwise)

Preceding gene: 119945323

Following gene: 119945325

Centisome position: 43.05

GC content: 41.67

Gene sequence:

>840_bases
TTGTCTATTGACGACGCGCTTGCTTGGGCAAAGTTTACTCTGGTCAACAGCGAAAGTGCGTTATTAGATGCGCAGGTACT
GTTAGCGTTTGTTTTAGAAAAAGAAACCGTTTACCTGATGACTTGGCCTGAGCGTGAGCTTAGCCAAGCGCAAAAAAGTG
CTTTTGAGGGACTAATTAGTCAGCGAGTAAAAGGTGTTCCGGTTGCCCATTTAACGGGTGCCAGAGAGTTTTGGTCGTTG
CCTTTAAAAGTGAATAATTCAACATTGATACCGCGCCCTGACACTGAACTATTGGTTGAAGCTGCCCTTGACTATTGCAG
CGCCGATGCCCGTATTTTAGATCTCGGTACAGGGAGTGGGGCGATTATTTTGGCACTAGCCAGTGAATTACCTGATGCCT
ATTGTCTGGGTGTTGATGTTAATGAAAGCGCGGTGCAGCTGGCGATTGAAAATGGAAAAAATTTAAAGCTTAACAATGTT
CATTTCCAACAGAGCAATTGGTTTGATAATATTAACGGCTTGTTTGATGTGGTTGTTTCAAATCCGCCTTATATTGAAAA
AAATGATCATCACCTTAAGCTGGGAGATGTTCGTTTTGAGCCTTTGTCGGCTTTAGTGGCGGATGAAAATGGTCTGGCAG
ATATTCGTAAAATTGCACAAAAAACGCCGGAATATCTTAAAAAAGGGGGCTATTTATTGGTTGAACATGGTTTTAACCAA
GGTTCAACAGTTCGAGAATTATTCACTGACCTAGGTTATTCAGAGGTTATTACCATTAAAGACTACGGAGATAATGATCG
CGTTACGATGGGATTGTTTAACCAACCTAATTCACTATAA

Upstream 100 bases:

>100_bases
GAATGAGGTGTTAGAAGGTGATTTAAATGCCTTGCATGAGCCTATTTTGCTGGAAGATCAAGCCGATAAACTCGCGGCAC
TTTCTCAGGCGGAATTCTAA

Downstream 100 bases:

>100_bases
AAATAAACCTAATACTTGGAGAAATAGATGTATAGCTTTTTAAAACATACTCATTTAACGTTGATATTAATAGCGATTGT
GCTTTTTATCTTATGTTTTT

Product: modification methylase, HemK family protein

Products: NA

Alternate protein names: M.VchAHemK2P [H]

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MSIDDALAWAKFTLVNSESALLDAQVLLAFVLEKETVYLMTWPERELSQAQKSAFEGLISQRVKGVPVAHLTGAREFWSL
PLKVNNSTLIPRPDTELLVEAALDYCSADARILDLGTGSGAIILALASELPDAYCLGVDVNESAVQLAIENGKNLKLNNV
HFQQSNWFDNINGLFDVVVSNPPYIEKNDHHLKLGDVRFEPLSALVADENGLADIRKIAQKTPEYLKKGGYLLVEHGFNQ
GSTVRELFTDLGYSEVITIKDYGDNDRVTMGLFNQPNSL

Sequences:

>Translated_279_residues
MSIDDALAWAKFTLVNSESALLDAQVLLAFVLEKETVYLMTWPERELSQAQKSAFEGLISQRVKGVPVAHLTGAREFWSL
PLKVNNSTLIPRPDTELLVEAALDYCSADARILDLGTGSGAIILALASELPDAYCLGVDVNESAVQLAIENGKNLKLNNV
HFQQSNWFDNINGLFDVVVSNPPYIEKNDHHLKLGDVRFEPLSALVADENGLADIRKIAQKTPEYLKKGGYLLVEHGFNQ
GSTVRELFTDLGYSEVITIKDYGDNDRVTMGLFNQPNSL
>Mature_278_residues
SIDDALAWAKFTLVNSESALLDAQVLLAFVLEKETVYLMTWPERELSQAQKSAFEGLISQRVKGVPVAHLTGAREFWSLP
LKVNNSTLIPRPDTELLVEAALDYCSADARILDLGTGSGAIILALASELPDAYCLGVDVNESAVQLAIENGKNLKLNNVH
FQQSNWFDNINGLFDVVVSNPPYIEKNDHHLKLGDVRFEPLSALVADENGLADIRKIAQKTPEYLKKGGYLLVEHGFNQG
STVRELFTDLGYSEVITIKDYGDNDRVTMGLFNQPNSL

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=234, Percent_Identity=32.0512820512821, Blast_Score=92, Evalue=4e-19,
Organism=Escherichia coli, GI1787463, Length=264, Percent_Identity=50.3787878787879, Blast_Score=261, Evalue=5e-71,
Organism=Escherichia coli, GI87082085, Length=214, Percent_Identity=30.3738317757009, Blast_Score=83, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324266, Length=163, Percent_Identity=30.6748466257669, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24582226, Length=214, Percent_Identity=28.5046728971963, Blast_Score=88, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 30795; Mature: 30664

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIDDALAWAKFTLVNSESALLDAQVLLAFVLEKETVYLMTWPERELSQAQKSAFEGLIS
CCCHHHHHEEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHH
QRVKGVPVAHLTGAREFWSLPLKVNNSTLIPRPDTELLVEAALDYCSADARILDLGTGSG
HHHCCCCEEEECCCHHHEECCEEECCCEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCC
AIILALASELPDAYCLGVDVNESAVQLAIENGKNLKLNNVHFQQSNWFDNINGLFDVVVS
EEEEEEEHHCCCEEEEEECCCCHHEEEEEECCCCEEEEEEEEECCCCCCCCCCEEEEEEC
NPPYIEKNDHHLKLGDVRFEPLSALVADENGLADIRKIAQKTPEYLKKGGYLLVEHGFNQ
CCCCCCCCCCEEEECCEEECCHHHEEECCCCHHHHHHHHHCCHHHHHCCCEEEEEECCCC
GSTVRELFTDLGYSEVITIKDYGDNDRVTMGLFNQPNSL
CHHHHHHHHHCCCCEEEEEEECCCCCCEEEEECCCCCCC
>Mature Secondary Structure 
SIDDALAWAKFTLVNSESALLDAQVLLAFVLEKETVYLMTWPERELSQAQKSAFEGLIS
CCHHHHHEEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHH
QRVKGVPVAHLTGAREFWSLPLKVNNSTLIPRPDTELLVEAALDYCSADARILDLGTGSG
HHHCCCCEEEECCCHHHEECCEEECCCEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCC
AIILALASELPDAYCLGVDVNESAVQLAIENGKNLKLNNVHFQQSNWFDNINGLFDVVVS
EEEEEEEHHCCCEEEEEECCCCHHEEEEEECCCCEEEEEEEEECCCCCCCCCCEEEEEEC
NPPYIEKNDHHLKLGDVRFEPLSALVADENGLADIRKIAQKTPEYLKKGGYLLVEHGFNQ
CCCCCCCCCCEEEECCEEECCHHHEEECCCCHHHHHHHHHCCHHHHHCCCEEEEEECCCC
GSTVRELFTDLGYSEVITIKDYGDNDRVTMGLFNQPNSL
CHHHHHHHHHCCCCEEEEEEECCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]