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The map label for this gene is prfA

Identifier: 119945323

GI number: 119945323

Start: 1961871

End: 1962962

Strand: Direct

Name: prfA

Synonym: Ping_1607

Alternate gene names: 119945323

Gene position: 1961871-1962962 (Clockwise)

Preceding gene: 119945322

Following gene: 119945324

Centisome position: 43.03

GC content: 45.24

Gene sequence:

>1092_bases
ATGAAAGCATCAATAGTGGCAAAGCTCGAAGTCCTAGTTGAACGTTACGAAGAGGTTCAAGCATTATTAGGTGATCCGGG
TACGATCAGTGATCAAAATAAATACAGAGAGTTAACCAAAGAGTATGCACAACTGGAAGTTGTGGTACTTTCCTTTAAAA
AATATCAAAGTGCCCAAAATGATCTTGCCGTTGCTGAAATGATGTCCGATGATGATGATCCCGATATGCGTGAAATGGCC
AAAGAAGAAATTAAAGAAGCTAAAAAAACGGTGGAAAAATTGGCTGCCGATTTACAGATTCTACTGCTGCCAAAGGATCC
TAATGATGATCGTAATTGTTATTTAGAAATTAGAGCGGGTGCCGGAGGTGACGAAGCAGCCATTTTTGCGGGCAATCTGT
TCCGTATGTACAGTAAATATGCTGAAAGCAAAGGTTGGCGAGTTGAGGTGATGAACAGCAATGCCAGCGAGCAGGGCGGT
TACAAAGAATTAATTGCTAAGATTGATGGTGAGGGCGCATACGGGATTATGAAATTTGAGTCTGGCGGTCATCGTGTGCA
GCGTGTGCCGGAAACTGAATCTCAAGGTCGTATTCATACCTCCGCTTGTACCGTTGTGGTTATGCCGGAAGTACCTGAAG
CCGAGGCCATTTCCATTAATCCTGCTGATCTTAAAGTTGATACCTTCCGTGCTTCTGGCGCGGGTGGACAGCATGTTAAT
AAAACCGATTCAGCGGTGCGATTAACTCACTTGCCAACCGGTACCGTGGTCGAGTGTCAGGATCAGCGTTCACAGCATAA
AAACAGAGCACAAGCTATGTCTGTGTTACAGTCGCGTCTGCAGCAGGCTGAGGATGAAAAGAGCCATGCTGAAGAACAAA
CTATTCGCCGCAGCTTAGTTGCAAGTGGTGATCGCAGTGAGCGTATTCGTACCTACAATTATCCGCAGGGACGTGTCAGT
GACCACCGTATCAACTTAACGGTTTACCGTTTGAATGAGGTGTTAGAAGGTGATTTAAATGCCTTGCATGAGCCTATTTT
GCTGGAAGATCAAGCCGATAAACTCGCGGCACTTTCTCAGGCGGAATTCTAA

Upstream 100 bases:

>100_bases
TGATGAACTGATATTGTTGCGTAATGCTTTGGGCCTGAGCAAAAAAGATTAACATTCGCTGTACACTCAGCAAATGAATA
AAAGAACATAAGAGAAAAAT

Downstream 100 bases:

>100_bases
TTGTCTATTGACGACGCGCTTGCTTGGGCAAAGTTTACTCTGGTCAACAGCGAAAGTGCGTTATTAGATGCGCAGGTACT
GTTAGCGTTTGTTTTAGAAA

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MKASIVAKLEVLVERYEEVQALLGDPGTISDQNKYRELTKEYAQLEVVVLSFKKYQSAQNDLAVAEMMSDDDDPDMREMA
KEEIKEAKKTVEKLAADLQILLLPKDPNDDRNCYLEIRAGAGGDEAAIFAGNLFRMYSKYAESKGWRVEVMNSNASEQGG
YKELIAKIDGEGAYGIMKFESGGHRVQRVPETESQGRIHTSACTVVVMPEVPEAEAISINPADLKVDTFRASGAGGQHVN
KTDSAVRLTHLPTGTVVECQDQRSQHKNRAQAMSVLQSRLQQAEDEKSHAEEQTIRRSLVASGDRSERIRTYNYPQGRVS
DHRINLTVYRLNEVLEGDLNALHEPILLEDQADKLAALSQAEF

Sequences:

>Translated_363_residues
MKASIVAKLEVLVERYEEVQALLGDPGTISDQNKYRELTKEYAQLEVVVLSFKKYQSAQNDLAVAEMMSDDDDPDMREMA
KEEIKEAKKTVEKLAADLQILLLPKDPNDDRNCYLEIRAGAGGDEAAIFAGNLFRMYSKYAESKGWRVEVMNSNASEQGG
YKELIAKIDGEGAYGIMKFESGGHRVQRVPETESQGRIHTSACTVVVMPEVPEAEAISINPADLKVDTFRASGAGGQHVN
KTDSAVRLTHLPTGTVVECQDQRSQHKNRAQAMSVLQSRLQQAEDEKSHAEEQTIRRSLVASGDRSERIRTYNYPQGRVS
DHRINLTVYRLNEVLEGDLNALHEPILLEDQADKLAALSQAEF
>Mature_363_residues
MKASIVAKLEVLVERYEEVQALLGDPGTISDQNKYRELTKEYAQLEVVVLSFKKYQSAQNDLAVAEMMSDDDDPDMREMA
KEEIKEAKKTVEKLAADLQILLLPKDPNDDRNCYLEIRAGAGGDEAAIFAGNLFRMYSKYAESKGWRVEVMNSNASEQGG
YKELIAKIDGEGAYGIMKFESGGHRVQRVPETESQGRIHTSACTVVVMPEVPEAEAISINPADLKVDTFRASGAGGQHVN
KTDSAVRLTHLPTGTVVECQDQRSQHKNRAQAMSVLQSRLQQAEDEKSHAEEQTIRRSLVASGDRSERIRTYNYPQGRVS
DHRINLTVYRLNEVLEGDLNALHEPILLEDQADKLAALSQAEF

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=280, Percent_Identity=46.7857142857143, Blast_Score=266, Evalue=2e-71,
Organism=Homo sapiens, GI34577120, Length=339, Percent_Identity=39.2330383480826, Blast_Score=236, Evalue=3e-62,
Organism=Homo sapiens, GI166795305, Length=195, Percent_Identity=47.1794871794872, Blast_Score=188, Evalue=5e-48,
Organism=Escherichia coli, GI1787462, Length=362, Percent_Identity=66.5745856353591, Blast_Score=496, Evalue=1e-141,
Organism=Escherichia coli, GI2367172, Length=337, Percent_Identity=39.1691394658754, Blast_Score=224, Evalue=9e-60,
Organism=Caenorhabditis elegans, GI17542784, Length=269, Percent_Identity=36.4312267657993, Blast_Score=176, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6321295, Length=343, Percent_Identity=41.9825072886297, Blast_Score=252, Evalue=6e-68,
Organism=Drosophila melanogaster, GI19921226, Length=320, Percent_Identity=42.1875, Blast_Score=228, Evalue=5e-60,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_PSYIN (A1SV89)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_943003.1
- ProteinModelPortal:   A1SV89
- SMR:   A1SV89
- STRING:   A1SV89
- GeneID:   4624636
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_1607
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- PhylomeDB:   A1SV89
- BioCyc:   PING357804:PING_1607-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40458; Mature: 40458

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASIVAKLEVLVERYEEVQALLGDPGTISDQNKYRELTKEYAQLEVVVLSFKKYQSAQN
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHEEEHHHHHCHHH
DLAVAEMMSDDDDPDMREMAKEEIKEAKKTVEKLAADLQILLLPKDPNDDRNCYLEIRAG
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEEEEEC
AGGDEAAIFAGNLFRMYSKYAESKGWRVEVMNSNASEQGGYKELIAKIDGEGAYGIMKFE
CCCCCCEEEHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHCCCCCCEEEEEEC
SGGHRVQRVPETESQGRIHTSACTVVVMPEVPEAEAISINPADLKVDTFRASGAGGQHVN
CCCCCEEECCCCCCCCEEEECEEEEEEECCCCCCCEEECCCCCEEEEEEEECCCCCCCCC
KTDSAVRLTHLPTGTVVECQDQRSQHKNRAQAMSVLQSRLQQAEDEKSHAEEQTIRRSLV
CCCCEEEEEECCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASGDRSERIRTYNYPQGRVSDHRINLTVYRLNEVLEGDLNALHEPILLEDQADKLAALSQ
HCCCCCCCCEECCCCCCCCCCCEEEEEEEEHHHHHHHHHHHHCCCEEEECCHHHHHHHHH
AEF
CCC
>Mature Secondary Structure
MKASIVAKLEVLVERYEEVQALLGDPGTISDQNKYRELTKEYAQLEVVVLSFKKYQSAQN
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHEEEHHHHHCHHH
DLAVAEMMSDDDDPDMREMAKEEIKEAKKTVEKLAADLQILLLPKDPNDDRNCYLEIRAG
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEEEEEC
AGGDEAAIFAGNLFRMYSKYAESKGWRVEVMNSNASEQGGYKELIAKIDGEGAYGIMKFE
CCCCCCEEEHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHCCCCCCEEEEEEC
SGGHRVQRVPETESQGRIHTSACTVVVMPEVPEAEAISINPADLKVDTFRASGAGGQHVN
CCCCCEEECCCCCCCCEEEECEEEEEEECCCCCCCEEECCCCCEEEEEEEECCCCCCCCC
KTDSAVRLTHLPTGTVVECQDQRSQHKNRAQAMSVLQSRLQQAEDEKSHAEEQTIRRSLV
CCCCEEEEEECCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASGDRSERIRTYNYPQGRVSDHRINLTVYRLNEVLEGDLNALHEPILLEDQADKLAALSQ
HCCCCCCCCEECCCCCCCCCCCEEEEEEEEHHHHHHHHHHHHCCCEEEECCHHHHHHHHH
AEF
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA