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The map label for this gene is asnB [H]

Identifier: 119944862

GI number: 119944862

Start: 1377823

End: 1379493

Strand: Reverse

Name: asnB [H]

Synonym: Ping_1104

Alternate gene names: 119944862

Gene position: 1379493-1377823 (Counterclockwise)

Preceding gene: 119944863

Following gene: 119944861

Centisome position: 30.25

GC content: 41.65

Gene sequence:

>1671_bases
ATGTGTTCTATTTTTGGTGTACTTGACATTAAAAGCGATCCGATCGCTTTACGCCAACAAGCTATTGAAATGTCTAAACT
ACTTCGTCACCGTGGCCCCGATTGGTCCGGTGTTTACTCGTCTGATAAAGCAATACTGGTTCATGAGCGTCTTTCCATTG
TAGATGTCAATACCGGCGCACAACCGCTGTACAATCAGGATAATACACATATCCTTGCAGTGAATGGTGAAATTTATAAC
CACAAAGCGTTAGAAGCTGAATTAACCTGTGGTTATCAGTTTAAAACAAAATCAGACTGTGAAATTATCTTAGCTTTGTA
CGAAGAAAAAGGGTGTGATTTTCTGGATGATTTAAATGGTATCTTTGCTTTTTGTTTATACGATGCCAAAAAAGATAAAT
ACCTGATTGGCCGTGATCATATGGGTATTATCCCGCTCTATATGGGCTGGGATGAACATGGCAATTTTTATGTGGCGTCA
GAAATGAAAGCATTAACACCTATCTGCACAAAAATTCAGGAATTCCCAACGGGATCCTTTTTAGACAGTGAAGTGGGTGA
AATTACTCAATATTACGCGCGTGACTGGATGGAATATGATGCAGTTAAAGATAACGGCGGCAGCGCGGCTGAAATTGGCG
AAGCACTTGAAGCATCTGTTAAGCGTCAATTAATGTGTGATGTACCCTACGGTGTTTTACTGTCCGGTGGATTAGATTCA
TCTGTTATCTCGGCTATCACGCAAAAATTTGCTAAACAGCGTATTGAAGATAATGGTGAAAGCGGCGCATGGTGGCCACA
ACTGCACTCTTTTTCTGTCGGACTTGAAGGCTCGCCTGATTTAGCCGCCGCTGCAAAAGTAGCCAAAGAATTAGGCACTA
TTCACCACCCTATTATCTTTACCGTACAAAATGGTATCGATGCGTTGCGTGAAGTTATTTATCATATTGAAACTTACGAT
GTAACCACTATCCGCGCTGCGACACCTATGTACCTAATGGCACGTAAGATTAAGGCGATGGGCATAAAAATGGTTCTTTC
AGGTGAAGGAGCTGATGAAATATTTGCCGGTTACTTGTACTTCCACAAAGCGCCAAATCCAAAAGAATTACATGAAGAAT
TAAACCGTAAACTCAGTAAACTGCATATGTTTGACTGTCTACGTGCTAATAAATCAATGGCGGCTTGGGGCATTGAGGCA
CGCGTACCTTTCTTAGATAAAGAGTTTATGGATGTCGCTATGCGTACCAATCCTGAACTTAAAATGATCAAAGATGGCCG
TATTGAAAAAAACATTCTTCGTGAAGCCTTTGAAGGTCAGCTGTTAAGTGAAGTTTTATGGCGTCAAAAAGAGCAGTTCT
CCGATGGTGTCGGTTACAACTGGATAGATACGTTAAAAGCGCATATTGAAACGCAAGTAACAGATCAACAATTAGAAACT
GCGCAGTTCAAGTTTCCAATTAATACCCCCGATACCAAAGAAGCTTACTTCTACCGTACTATCTTTGCCGAGCACTTCCC
GCTGGACAGTGCTGCAGAATGTGTACCGCACGGTAAATCAGTTGCCTGTTCAACCGTTGAAGCATTAGCCTGGGATGAGA
GTTTCCAAAATAATGCAGATCCTTCTGGTCGTGCAGCAGGCATTCATAATGATGCATACAATCTTGCCTAA

Upstream 100 bases:

>100_bases
TCAAATTATTGTTTTTTGTTTTTTCACAGTAAATGGACAAACATTAATAACTTAGCACTCGATATCTTGTAGATATCCGC
TTTAAATAAAAGGTAACATT

Downstream 100 bases:

>100_bases
CTAAATGTAAATGCTTAGTTAACTTTATAAAGCGTTCCGCGGAACGCTTTTTTTATACGCAGCCGAATATAAAATGTCTA
AATTAAATCAGCAAACCTGC

Product: asparagine synthetase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 556; Mature: 556

Protein sequence:

>556_residues
MCSIFGVLDIKSDPIALRQQAIEMSKLLRHRGPDWSGVYSSDKAILVHERLSIVDVNTGAQPLYNQDNTHILAVNGEIYN
HKALEAELTCGYQFKTKSDCEIILALYEEKGCDFLDDLNGIFAFCLYDAKKDKYLIGRDHMGIIPLYMGWDEHGNFYVAS
EMKALTPICTKIQEFPTGSFLDSEVGEITQYYARDWMEYDAVKDNGGSAAEIGEALEASVKRQLMCDVPYGVLLSGGLDS
SVISAITQKFAKQRIEDNGESGAWWPQLHSFSVGLEGSPDLAAAAKVAKELGTIHHPIIFTVQNGIDALREVIYHIETYD
VTTIRAATPMYLMARKIKAMGIKMVLSGEGADEIFAGYLYFHKAPNPKELHEELNRKLSKLHMFDCLRANKSMAAWGIEA
RVPFLDKEFMDVAMRTNPELKMIKDGRIEKNILREAFEGQLLSEVLWRQKEQFSDGVGYNWIDTLKAHIETQVTDQQLET
AQFKFPINTPDTKEAYFYRTIFAEHFPLDSAAECVPHGKSVACSTVEALAWDESFQNNADPSGRAAGIHNDAYNLA

Sequences:

>Translated_556_residues
MCSIFGVLDIKSDPIALRQQAIEMSKLLRHRGPDWSGVYSSDKAILVHERLSIVDVNTGAQPLYNQDNTHILAVNGEIYN
HKALEAELTCGYQFKTKSDCEIILALYEEKGCDFLDDLNGIFAFCLYDAKKDKYLIGRDHMGIIPLYMGWDEHGNFYVAS
EMKALTPICTKIQEFPTGSFLDSEVGEITQYYARDWMEYDAVKDNGGSAAEIGEALEASVKRQLMCDVPYGVLLSGGLDS
SVISAITQKFAKQRIEDNGESGAWWPQLHSFSVGLEGSPDLAAAAKVAKELGTIHHPIIFTVQNGIDALREVIYHIETYD
VTTIRAATPMYLMARKIKAMGIKMVLSGEGADEIFAGYLYFHKAPNPKELHEELNRKLSKLHMFDCLRANKSMAAWGIEA
RVPFLDKEFMDVAMRTNPELKMIKDGRIEKNILREAFEGQLLSEVLWRQKEQFSDGVGYNWIDTLKAHIETQVTDQQLET
AQFKFPINTPDTKEAYFYRTIFAEHFPLDSAAECVPHGKSVACSTVEALAWDESFQNNADPSGRAAGIHNDAYNLA
>Mature_556_residues
MCSIFGVLDIKSDPIALRQQAIEMSKLLRHRGPDWSGVYSSDKAILVHERLSIVDVNTGAQPLYNQDNTHILAVNGEIYN
HKALEAELTCGYQFKTKSDCEIILALYEEKGCDFLDDLNGIFAFCLYDAKKDKYLIGRDHMGIIPLYMGWDEHGNFYVAS
EMKALTPICTKIQEFPTGSFLDSEVGEITQYYARDWMEYDAVKDNGGSAAEIGEALEASVKRQLMCDVPYGVLLSGGLDS
SVISAITQKFAKQRIEDNGESGAWWPQLHSFSVGLEGSPDLAAAAKVAKELGTIHHPIIFTVQNGIDALREVIYHIETYD
VTTIRAATPMYLMARKIKAMGIKMVLSGEGADEIFAGYLYFHKAPNPKELHEELNRKLSKLHMFDCLRANKSMAAWGIEA
RVPFLDKEFMDVAMRTNPELKMIKDGRIEKNILREAFEGQLLSEVLWRQKEQFSDGVGYNWIDTLKAHIETQVTDQQLET
AQFKFPINTPDTKEAYFYRTIFAEHFPLDSAAECVPHGKSVACSTVEALAWDESFQNNADPSGRAAGIHNDAYNLA

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=582, Percent_Identity=38.1443298969072, Blast_Score=323, Evalue=3e-88,
Organism=Homo sapiens, GI168229252, Length=582, Percent_Identity=38.1443298969072, Blast_Score=323, Evalue=3e-88,
Organism=Homo sapiens, GI168229250, Length=582, Percent_Identity=38.1443298969072, Blast_Score=323, Evalue=3e-88,
Organism=Homo sapiens, GI296010848, Length=553, Percent_Identity=39.0596745027125, Blast_Score=322, Evalue=7e-88,
Organism=Homo sapiens, GI296010852, Length=487, Percent_Identity=39.4250513347023, Blast_Score=295, Evalue=1e-79,
Organism=Homo sapiens, GI296010850, Length=487, Percent_Identity=39.4250513347023, Blast_Score=295, Evalue=1e-79,
Organism=Escherichia coli, GI1786889, Length=554, Percent_Identity=71.2996389891697, Blast_Score=850, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25147557, Length=576, Percent_Identity=41.6666666666667, Blast_Score=421, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI71993933, Length=562, Percent_Identity=41.9928825622776, Blast_Score=410, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17560178, Length=535, Percent_Identity=35.1401869158879, Blast_Score=296, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI25147560, Length=314, Percent_Identity=36.624203821656, Blast_Score=180, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6321563, Length=581, Percent_Identity=47.8485370051635, Blast_Score=518, Evalue=1e-147,
Organism=Saccharomyces cerevisiae, GI6325403, Length=582, Percent_Identity=46.9072164948454, Blast_Score=503, Evalue=1e-143,
Organism=Drosophila melanogaster, GI45553209, Length=575, Percent_Identity=44.3478260869565, Blast_Score=471, Evalue=1e-133,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 62366; Mature: 62366

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCSIFGVLDIKSDPIALRQQAIEMSKLLRHRGPDWSGVYSSDKAILVHERLSIVDVNTGA
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCEEEEEECCCC
QPLYNQDNTHILAVNGEIYNHKALEAELTCGYQFKTKSDCEIILALYEEKGCDFLDDLNG
CCCCCCCCCEEEEECCCEECCCCEEEEEEECEEECCCCCCEEEEEEECCCCCCHHHHHHH
IFAFCLYDAKKDKYLIGRDHMGIIPLYMGWDEHGNFYVASEMKALTPICTKIQEFPTGSF
HHHHHHEECCCCCEEECCCCCCEEEEEECCCCCCCEEEECCHHHHHHHHHHHHHCCCCCC
LDSEVGEITQYYARDWMEYDAVKDNGGSAAEIGEALEASVKRQLMCDVPYGVLLSGGLDS
CHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHEEECCCCH
SVISAITQKFAKQRIEDNGESGAWWPQLHSFSVGLEGSPDLAAAAKVAKELGTIHHPIIF
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHCCCCCCEEE
TVQNGIDALREVIYHIETYDVTTIRAATPMYLMARKIKAMGIKMVLSGEGADEIFAGYLY
EEHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCEEEEEECCCHHHHHEEEEE
FHKAPNPKELHEELNRKLSKLHMFDCLRANKSMAAWGIEARVPFLDKEFMDVAMRTNPEL
EECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCEECCCCCCHHHHHHHHCCCCCE
KMIKDGRIEKNILREAFEGQLLSEVLWRQKEQFSDGVGYNWIDTLKAHIETQVTDQQLET
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
AQFKFPINTPDTKEAYFYRTIFAEHFPLDSAAECVPHGKSVACSTVEALAWDESFQNNAD
EEEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHCHHHCCCCC
PSGRAAGIHNDAYNLA
CCCCCCCCCCCCCCCC
>Mature Secondary Structure
MCSIFGVLDIKSDPIALRQQAIEMSKLLRHRGPDWSGVYSSDKAILVHERLSIVDVNTGA
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCEEEEEECCCC
QPLYNQDNTHILAVNGEIYNHKALEAELTCGYQFKTKSDCEIILALYEEKGCDFLDDLNG
CCCCCCCCCEEEEECCCEECCCCEEEEEEECEEECCCCCCEEEEEEECCCCCCHHHHHHH
IFAFCLYDAKKDKYLIGRDHMGIIPLYMGWDEHGNFYVASEMKALTPICTKIQEFPTGSF
HHHHHHEECCCCCEEECCCCCCEEEEEECCCCCCCEEEECCHHHHHHHHHHHHHCCCCCC
LDSEVGEITQYYARDWMEYDAVKDNGGSAAEIGEALEASVKRQLMCDVPYGVLLSGGLDS
CHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHEEECCCCH
SVISAITQKFAKQRIEDNGESGAWWPQLHSFSVGLEGSPDLAAAAKVAKELGTIHHPIIF
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHCCCCCCEEE
TVQNGIDALREVIYHIETYDVTTIRAATPMYLMARKIKAMGIKMVLSGEGADEIFAGYLY
EEHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCEEEEEECCCHHHHHEEEEE
FHKAPNPKELHEELNRKLSKLHMFDCLRANKSMAAWGIEARVPFLDKEFMDVAMRTNPEL
EECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCEECCCCCCHHHHHHHHCCCCCE
KMIKDGRIEKNILREAFEGQLLSEVLWRQKEQFSDGVGYNWIDTLKAHIETQVTDQQLET
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
AQFKFPINTPDTKEAYFYRTIFAEHFPLDSAAECVPHGKSVACSTVEALAWDESFQNNAD
EEEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHCHHHCCCCC
PSGRAAGIHNDAYNLA
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1973930; 8905232; 9278503; 10587437 [H]