Click here to switch to the map view.

The map label for this gene is gatA [H]

Identifier: 119944197

GI number: 119944197

Start: 527757

End: 529070

Strand: Reverse

Name: gatA [H]

Synonym: Ping_0417

Alternate gene names: 119944197

Gene position: 529070-527757 (Counterclockwise)

Preceding gene: 119944200

Following gene: 119944196

Centisome position: 11.6

GC content: 43.23

Gene sequence:

>1314_bases
ATGCACCACGTAACTGCAAAACCAACAGCATACAGCATCGCAATTAATTGGTTAACTAGTCATATTGTTTATTACCGGAT
AGTAATTATGTTAACTAACCTTCCTTTTACTATCGATGCTTTGCACGAGGTCTGTCGTCAAGGAGTCAGTCCTGATGAAG
TGATCCAAGAATCACTAGCGCGTCTTACGAATATCGCAGACGAAGGTATTTATTTCTATGTTGCCAGCAGTTCGCAGTTG
TCGAAAGCTATCACAGACCTTGGTCCCTTTGATCTTGAGCTAAAACCGTTATGGGGAATTCCGTTTGCTGTCAAAGATAA
TATTGATGCAGCGAATATGCCAACGACTGCCGCCTGTTATGAGTTTCATTATATTACGAAGCAAGATGCTTATGTGGTTT
CTCTATTGAAACTGGCAGGGGCAATAGTCATTGGTAAAACCAATCTTGATCAATTTGCAACTGGCTTAGTGGGCTTAAGA
ACACCTTATCCCGCACCTAAAAATGCGATTGATTCAAAACTGGTACCCGATAGATCTAGTTTGCGTTCAGCATTGGCAGT
GGCACATGGGGAGGTCTACTTTTCGCTTGGAACTGATACTGCCGGTTCGGGGCGTATAACTTCAGCACTCAATAATATTG
TGGGTCTCAAACCGACGCTTGGTAGTATCTCTAGCCGAGGAGTGATTCCCACCTGTCGTTCACTCGATACAGTATCGATA
TTAGCGCTAACGGTTGAAGATGCTTCACTAGTGTATGCCTGTTGTCGAAGCTACGATAAAAAAGATCCCTTTTCTAAACC
CTATCACGGTGATGCTAAGCAGAATATGCCCACTGATTTTACCGTTGCGGTACCCGATTCAACCAGCCTGATTCTGGATG
AACCCGCTCAAAAGAAAGCTTATGAGCAAGCCCTTAACTGCTGGCGACAAAAAAGAGCAAAGCTGGTTGAAATTGACTTT
AACCCATTCTATGAGGTGCCTAAGTTATTATATGAAGGCCTCTGGGTTGCAGAAAGACTAGCGGCATTAGAGCATTTTAT
TGCAAAGCATCAGAGTGCGCTATATTCGATAACTCATGTGATCATTGAATCTGCGCACAAGTTCTCCGCTAGTGATGCAT
TTAATGCCCGCTATCACTTGCAGGCACTTCCCTGGAAAACAACCTCAATATTAACAAAGATCGACGTAATCGGTGTTCCG
CCGATGCCAGGACTAGTTTACAAAAGTGACATTGGGTCCAACGCTATAGGCCCAAATAGTCGTCTCGGAACTTACACTAA
CTTGCTCGATGATGATCATAAAAAATATCATTAG

Upstream 100 bases:

>100_bases
TGAAAATAATTAAAATCCATAAGTTGCTTCATTTATATTCTTCCATTAGTAAAAGTTTGGATAATTGCTCCATTGAACAC
TTATCCATGTGATGCAAAAT

Downstream 100 bases:

>100_bases
GTAATCTAAAACTTTTTTAATCTTTCTAGCTTATAGGATCCATACAGATGAAATCAAATAAGAATGCAGATATGCAAGAA
CCAAAAAACAAAGTGTCCCC

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MHHVTAKPTAYSIAINWLTSHIVYYRIVIMLTNLPFTIDALHEVCRQGVSPDEVIQESLARLTNIADEGIYFYVASSSQL
SKAITDLGPFDLELKPLWGIPFAVKDNIDAANMPTTAACYEFHYITKQDAYVVSLLKLAGAIVIGKTNLDQFATGLVGLR
TPYPAPKNAIDSKLVPDRSSLRSALAVAHGEVYFSLGTDTAGSGRITSALNNIVGLKPTLGSISSRGVIPTCRSLDTVSI
LALTVEDASLVYACCRSYDKKDPFSKPYHGDAKQNMPTDFTVAVPDSTSLILDEPAQKKAYEQALNCWRQKRAKLVEIDF
NPFYEVPKLLYEGLWVAERLAALEHFIAKHQSALYSITHVIIESAHKFSASDAFNARYHLQALPWKTTSILTKIDVIGVP
PMPGLVYKSDIGSNAIGPNSRLGTYTNLLDDDHKKYH

Sequences:

>Translated_437_residues
MHHVTAKPTAYSIAINWLTSHIVYYRIVIMLTNLPFTIDALHEVCRQGVSPDEVIQESLARLTNIADEGIYFYVASSSQL
SKAITDLGPFDLELKPLWGIPFAVKDNIDAANMPTTAACYEFHYITKQDAYVVSLLKLAGAIVIGKTNLDQFATGLVGLR
TPYPAPKNAIDSKLVPDRSSLRSALAVAHGEVYFSLGTDTAGSGRITSALNNIVGLKPTLGSISSRGVIPTCRSLDTVSI
LALTVEDASLVYACCRSYDKKDPFSKPYHGDAKQNMPTDFTVAVPDSTSLILDEPAQKKAYEQALNCWRQKRAKLVEIDF
NPFYEVPKLLYEGLWVAERLAALEHFIAKHQSALYSITHVIIESAHKFSASDAFNARYHLQALPWKTTSILTKIDVIGVP
PMPGLVYKSDIGSNAIGPNSRLGTYTNLLDDDHKKYH
>Mature_437_residues
MHHVTAKPTAYSIAINWLTSHIVYYRIVIMLTNLPFTIDALHEVCRQGVSPDEVIQESLARLTNIADEGIYFYVASSSQL
SKAITDLGPFDLELKPLWGIPFAVKDNIDAANMPTTAACYEFHYITKQDAYVVSLLKLAGAIVIGKTNLDQFATGLVGLR
TPYPAPKNAIDSKLVPDRSSLRSALAVAHGEVYFSLGTDTAGSGRITSALNNIVGLKPTLGSISSRGVIPTCRSLDTVSI
LALTVEDASLVYACCRSYDKKDPFSKPYHGDAKQNMPTDFTVAVPDSTSLILDEPAQKKAYEQALNCWRQKRAKLVEIDF
NPFYEVPKLLYEGLWVAERLAALEHFIAKHQSALYSITHVIIESAHKFSASDAFNARYHLQALPWKTTSILTKIDVIGVP
PMPGLVYKSDIGSNAIGPNSRLGTYTNLLDDDHKKYH

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=338, Percent_Identity=28.4023668639053, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17543272, Length=222, Percent_Identity=31.5315315315315, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6319685, Length=341, Percent_Identity=42.5219941348974, Blast_Score=250, Evalue=3e-67,
Organism=Saccharomyces cerevisiae, GI6323950, Length=173, Percent_Identity=39.8843930635838, Blast_Score=100, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24648113, Length=200, Percent_Identity=31.5, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 48093; Mature: 48093

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHHVTAKPTAYSIAINWLTSHIVYYRIVIMLTNLPFTIDALHEVCRQGVSPDEVIQESLA
CCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHH
RLTNIADEGIYFYVASSSQLSKAITDLGPFDLELKPLWGIPFAVKDNIDAANMPTTAACY
HHHHHCCCCEEEEEECCHHHHHHHHHCCCCCEEEEECCCCCEEEECCCCCCCCCCHHHHE
EFHYITKQDAYVVSLLKLAGAIVIGKTNLDQFATGLVGLRTPYPAPKNAIDSKLVPDRSS
EEEEECCCCHHHHHHHHHHCEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHH
LRSALAVAHGEVYFSLGTDTAGSGRITSALNNIVGLKPTLGSISSRGVIPTCRSLDTVSI
HHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEE
LALTVEDASLVYACCRSYDKKDPFSKPYHGDAKQNMPTDFTVAVPDSTSLILDEPAQKKA
EEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCEEECCCHHHHH
YEQALNCWRQKRAKLVEIDFNPFYEVPKLLYEGLWVAERLAALEHFIAKHQSALYSITHV
HHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIESAHKFSASDAFNARYHLQALPWKTTSILTKIDVIGVPPMPGLVYKSDIGSNAIGPNS
HHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHEEEEECCCCCCCEEEECCCCCCCCCCCC
RLGTYTNLLDDDHKKYH
CCCCHHHHHCCCHHCCC
>Mature Secondary Structure
MHHVTAKPTAYSIAINWLTSHIVYYRIVIMLTNLPFTIDALHEVCRQGVSPDEVIQESLA
CCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHH
RLTNIADEGIYFYVASSSQLSKAITDLGPFDLELKPLWGIPFAVKDNIDAANMPTTAACY
HHHHHCCCCEEEEEECCHHHHHHHHHCCCCCEEEEECCCCCEEEECCCCCCCCCCHHHHE
EFHYITKQDAYVVSLLKLAGAIVIGKTNLDQFATGLVGLRTPYPAPKNAIDSKLVPDRSS
EEEEECCCCHHHHHHHHHHCEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHH
LRSALAVAHGEVYFSLGTDTAGSGRITSALNNIVGLKPTLGSISSRGVIPTCRSLDTVSI
HHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEE
LALTVEDASLVYACCRSYDKKDPFSKPYHGDAKQNMPTDFTVAVPDSTSLILDEPAQKKA
EEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCEEECCCHHHHH
YEQALNCWRQKRAKLVEIDFNPFYEVPKLLYEGLWVAERLAALEHFIAKHQSALYSITHV
HHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIESAHKFSASDAFNARYHLQALPWKTTSILTKIDVIGVPPMPGLVYKSDIGSNAIGPNS
HHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHEEEEECCCCCCCEEEECCCCCCCCCCCC
RLGTYTNLLDDDHKKYH
CCCCHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA