Click here to switch to the map view.

The map label for this gene is birA [H]

Identifier: 119944005

GI number: 119944005

Start: 274322

End: 275290

Strand: Direct

Name: birA [H]

Synonym: Ping_0219

Alternate gene names: 119944005

Gene position: 274322-275290 (Clockwise)

Preceding gene: 119944004

Following gene: 119944008

Centisome position: 6.02

GC content: 39.42

Gene sequence:

>969_bases
ATGTCGGAACTGAATGAACAGGGTAATATTCTTTTAACGTTACTTGCCGATGGAGAGTTTCATTCGGGTGAAAAAATAGG
CGAACTTCTAGGTGTTTCGCGTACCTCGGTTAATAACTATATCAAAGGGTTACAAGGGATTGGCGTTGATATTTATAAAG
TCCCCGGGCGTGGTTATCAAAGTGCTACGCTGATTAAATTATTAAATGAGCAGAAAATTCGCCAACTTTGTGGGTATGAC
AATATAAAAGTTGAGCAAATTGTTGATTCGACAAATCAAATTTTATTAGATCAAATCCCTCATTTAGAAAATGGGCAAAC
ATGTATTGCTGAATATCAGTTAGCAGGAAGGGGAAGGCGAGGCCGTACTTGGGTTTCACCCTTTGCTTCTCATCTGTATT
TTTCATTTTATTGGTATTTCGATTCGGGTATAGATAAAATCTCGGGGCTGAGTTTACTGGTGGGTATCGCAGTAGTTAAT
ACGCTGGAAAAAATGGGCATACAGGGCGTTTCATTAAAGTGGCCAAACGATATTTATTACCAGGGAAAAAAATTGGCGGG
TATTTTAATTGAGTTAAATGCCCAGGCAACAGCCGCTTGCCACTGCGTGATAGGGATCGGTATTAATATCAAAATGCCGC
CTGAGCAGGCGAAATTAATAAACCAACCCTGGGCTGATTTAAATGCATTAATACCAACGGGTGTGGATCGCAACGAATTA
AGTGCCATTTTAATTAAAGAGCTGCAATATTTATTACCCCGTTATGAAAAATCTGGTTTAACGCCCTATTTATCCCGCTG
GTTTGAATTAGATTGTTTTTTAGATAAAAAAGTCAACTTACTGATTGCAGACACCATCACTATGGGCATCTGCCGCGGTG
TTAATAGACATGGCGCGCTGTTATTGGAAATAGAGGGTCAAGTTAAAGAGTTCATCGGTGGAGAGATTTCTTTACGTTTG
GCAAATTAA

Upstream 100 bases:

>100_bases
TGCGGACAACAGTAAAAGAAAAATTTAATATAAACTTAGAACACGAAGTGCGCTTTATCTGCGCGATGGGCGAGAGCAAC
TTAACAGAGGCGGTTAACAA

Downstream 100 bases:

>100_bases
CCGAGTTGGTTTTGATCCCCTTGAAAAGTCAGTATGTACAATGATCAGGGATTTTTATCACTTGCTGTTTTGCCCTGCTG
CCTGAATATAATTATTTTAT

Product: biotin--acetyl-CoA-carboxylase ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MSELNEQGNILLTLLADGEFHSGEKIGELLGVSRTSVNNYIKGLQGIGVDIYKVPGRGYQSATLIKLLNEQKIRQLCGYD
NIKVEQIVDSTNQILLDQIPHLENGQTCIAEYQLAGRGRRGRTWVSPFASHLYFSFYWYFDSGIDKISGLSLLVGIAVVN
TLEKMGIQGVSLKWPNDIYYQGKKLAGILIELNAQATAACHCVIGIGINIKMPPEQAKLINQPWADLNALIPTGVDRNEL
SAILIKELQYLLPRYEKSGLTPYLSRWFELDCFLDKKVNLLIADTITMGICRGVNRHGALLLEIEGQVKEFIGGEISLRL
AN

Sequences:

>Translated_322_residues
MSELNEQGNILLTLLADGEFHSGEKIGELLGVSRTSVNNYIKGLQGIGVDIYKVPGRGYQSATLIKLLNEQKIRQLCGYD
NIKVEQIVDSTNQILLDQIPHLENGQTCIAEYQLAGRGRRGRTWVSPFASHLYFSFYWYFDSGIDKISGLSLLVGIAVVN
TLEKMGIQGVSLKWPNDIYYQGKKLAGILIELNAQATAACHCVIGIGINIKMPPEQAKLINQPWADLNALIPTGVDRNEL
SAILIKELQYLLPRYEKSGLTPYLSRWFELDCFLDKKVNLLIADTITMGICRGVNRHGALLLEIEGQVKEFIGGEISLRL
AN
>Mature_321_residues
SELNEQGNILLTLLADGEFHSGEKIGELLGVSRTSVNNYIKGLQGIGVDIYKVPGRGYQSATLIKLLNEQKIRQLCGYDN
IKVEQIVDSTNQILLDQIPHLENGQTCIAEYQLAGRGRRGRTWVSPFASHLYFSFYWYFDSGIDKISGLSLLVGIAVVNT
LEKMGIQGVSLKWPNDIYYQGKKLAGILIELNAQATAACHCVIGIGINIKMPPEQAKLINQPWADLNALIPTGVDRNELS
AILIKELQYLLPRYEKSGLTPYLSRWFELDCFLDKKVNLLIADTITMGICRGVNRHGALLLEIEGQVKEFIGGEISLRLA
N

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=311, Percent_Identity=48.5530546623794, Blast_Score=305, Evalue=3e-84,
Organism=Drosophila melanogaster, GI24644246, Length=146, Percent_Identity=33.5616438356164, Blast_Score=68, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR004409
- InterPro:   IPR003142
- InterPro:   IPR004143
- InterPro:   IPR013196
- InterPro:   IPR008988
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB; PF08279 HTH_11 [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 35795; Mature: 35664

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELNEQGNILLTLLADGEFHSGEKIGELLGVSRTSVNNYIKGLQGIGVDIYKVPGRGYQ
CCCCCCCCCEEEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCEEEEECCCCCCC
SATLIKLLNEQKIRQLCGYDNIKVEQIVDSTNQILLDQIPHLENGQTCIAEYQLAGRGRR
HHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHCCCCCCCCEEEEEHHHHCCCCC
GRTWVSPFASHLYFSFYWYFDSGIDKISGLSLLVGIAVVNTLEKMGIQGVSLKWPNDIYY
CCCHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEE
QGKKLAGILIELNAQATAACHCVIGIGINIKMPPEQAKLINQPWADLNALIPTGVDRNEL
CCCEEEEEEEEECCCCCEEEEEEEEECEEEECCHHHHHHHCCCCHHHHHHCCCCCCHHHH
SAILIKELQYLLPRYEKSGLTPYLSRWFELDCFLDKKVNLLIADTITMGICRGVNRHGAL
HHHHHHHHHHHHHHHHCCCCCHHHHHHEEEEEEECCCCCEEEEHHHHHHHHCCCCCCCEE
LLEIEGQVKEFIGGEISLRLAN
EEEECCHHHHHCCCEEEEEECC
>Mature Secondary Structure 
SELNEQGNILLTLLADGEFHSGEKIGELLGVSRTSVNNYIKGLQGIGVDIYKVPGRGYQ
CCCCCCCCEEEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCEEEEECCCCCCC
SATLIKLLNEQKIRQLCGYDNIKVEQIVDSTNQILLDQIPHLENGQTCIAEYQLAGRGRR
HHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHCCCCCCCCEEEEEHHHHCCCCC
GRTWVSPFASHLYFSFYWYFDSGIDKISGLSLLVGIAVVNTLEKMGIQGVSLKWPNDIYY
CCCHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEE
QGKKLAGILIELNAQATAACHCVIGIGINIKMPPEQAKLINQPWADLNALIPTGVDRNEL
CCCEEEEEEEEECCCCCEEEEEEEEECEEEECCHHHHHHHCCCCHHHHHHCCCCCCHHHH
SAILIKELQYLLPRYEKSGLTPYLSRWFELDCFLDKKVNLLIADTITMGICRGVNRHGAL
HHHHHHHHHHHHHHHHCCCCCHHHHHHEEEEEEECCCCCEEEEHHHHHHHHCCCCCCCEE
LLEIEGQVKEFIGGEISLRLAN
EEEECCHHHHHCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3899863; 3536662; 8265357; 9278503; 1311302; 1328157; 1409631; 11353844 [H]