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The map label for this gene is nhaA2

Identifier: 119944008

GI number: 119944008

Start: 279687

End: 281114

Strand: Direct

Name: nhaA2

Synonym: Ping_0222

Alternate gene names: 119944008

Gene position: 279687-281114 (Clockwise)

Preceding gene: 119944005

Following gene: 119944014

Centisome position: 6.13

GC content: 44.68

Gene sequence:

>1428_bases
ATGAACCTTCGAATCGTTTATCTGCGCAGCTTAAATATACAAGGGCTTACTAAAATGACCGATGACAAAAATAATTTACT
GCACCGTGGCCTTGATAATATTCAGCCTCCTTTTTCAAACTTTATCCGCGCGCAGGCAACGGCCAGTGTGTTTCTTCTGG
TTGCAACGATTACTGCTCTGTGGTGGGCAAATTCAGATTATTCATCTACCTATCAGATGCTAAAGCATATGCAGATTGGT
TTTTTTCTGGGCGATATCGAGCTGCAGGCTTCACTTAAACATATTATTAATGATGGTTTGATGGTTATCTTTTTCTTCTT
TATCGGGCTTGAGATAAAACGTGAAGTGCTCGCCGGGGATCTCGCTATCGCAGAAAATCGGCGCATGTTGATTCTTTGTG
CTCTCGGTGGGATGATTTGTCCGGCTGTTATCTATTCATTATTTAACTGGTCACTTGATTCTCAGATTGGCTGGGGGATC
CCGATGGCAACCGATACGGCATTTGCATTAGGTGCGCTGACCCTAGTTAGAAAACATATTCCCATCAGTTTGTTAGCTTT
TCTCGTTGGCCTTGCCATTGTAGACGATGTTGGTGCGATAGTCGTGATTGCATTATTCTATACACAGGAGATATCTGTCA
TTTTCCTGTCGATCTCATTTTCCCTGATCGCGTTTTTGGCCATCGCTAATTATGCTGGTGTCCTCCGGCCAATTTTCTAT
ATTCTGATCGGGATTGCCGCTTGGTGGACGATGCTTAAATCTGGCGTTCATCCCACTTTTGCCGGCGTCGCCATTGCTTT
AACCGTACCCGCACGACCCATGTTACCGCCAGATGAATTACTCTCTAAGGTGAAAGCTAAAATTACAGCAATGAAGAAGA
ACAAAGGTCATCAACTCGATGCGCTTGCTAATCGAGAGGATCATGAACAAGTGCTGGATGTTCGTGATTTTGCAGAACAT
GGCAGTGCTCCCCTGCGCCGCTGGGAAGATGCATTGGACTTACCCGTCTCATTATTTGTCCTGCCACTGTTTGCTCTGGT
TAATGCCGGTGTGGAAGTTAGCTTCAGTTCACTTATCGAAACCCTTCAGCATTCAGTTGGGTTGGGTATTGTGATTGGGT
TGGTTATTGGTAAATTTGTTGGCATTTCCGGTGCATGCTGGCTTGGTCTGCGCTACAAAATAGGGAGTTTACCGGAGGGC
ATTAATATGCAGCACGTGATAGGTATGTCACTGATAGCCGGTATTGGCTTTACCATGTCGACATTTATAGCCACACTGGG
CTTTGACTCGCAACCTGAATATCTTCAAAGCGCCAAAGGATCTATTTTGTTCGCCTCACTTCTTTCTGCCATTCTGGGTC
TGTTATATCTCCGGATTATTGCTGCAAAAAAAGCACCCAATTCTGCATCAAAAAACGGAGAGAATTAG

Upstream 100 bases:

>100_bases
TAGAGACTTTTGCCGCGCGGCGTTATAGTTGTGGTTATGGTTGGCTGGCGTTAAGTTTTTATAAGTAGCGAGCTCTTAGC
CGGACCTTAGGTTCGATATA

Downstream 100 bases:

>100_bases
CCGCAATCTTATGTTAAGGAGTCCAGTGACTTATATTAAGATTTTAAAGGCAGCAATAGGAGTGCAGATAAAATATCACT
ATAATCGAACCGTCAAAGGT

Product: Na+/H+ antiporter NhaA

Products: Na (I) [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: Sodium/proton antiporter nhaA 2

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MNLRIVYLRSLNIQGLTKMTDDKNNLLHRGLDNIQPPFSNFIRAQATASVFLLVATITALWWANSDYSSTYQMLKHMQIG
FFLGDIELQASLKHIINDGLMVIFFFFIGLEIKREVLAGDLAIAENRRMLILCALGGMICPAVIYSLFNWSLDSQIGWGI
PMATDTAFALGALTLVRKHIPISLLAFLVGLAIVDDVGAIVVIALFYTQEISVIFLSISFSLIAFLAIANYAGVLRPIFY
ILIGIAAWWTMLKSGVHPTFAGVAIALTVPARPMLPPDELLSKVKAKITAMKKNKGHQLDALANREDHEQVLDVRDFAEH
GSAPLRRWEDALDLPVSLFVLPLFALVNAGVEVSFSSLIETLQHSVGLGIVIGLVIGKFVGISGACWLGLRYKIGSLPEG
INMQHVIGMSLIAGIGFTMSTFIATLGFDSQPEYLQSAKGSILFASLLSAILGLLYLRIIAAKKAPNSASKNGEN

Sequences:

>Translated_475_residues
MNLRIVYLRSLNIQGLTKMTDDKNNLLHRGLDNIQPPFSNFIRAQATASVFLLVATITALWWANSDYSSTYQMLKHMQIG
FFLGDIELQASLKHIINDGLMVIFFFFIGLEIKREVLAGDLAIAENRRMLILCALGGMICPAVIYSLFNWSLDSQIGWGI
PMATDTAFALGALTLVRKHIPISLLAFLVGLAIVDDVGAIVVIALFYTQEISVIFLSISFSLIAFLAIANYAGVLRPIFY
ILIGIAAWWTMLKSGVHPTFAGVAIALTVPARPMLPPDELLSKVKAKITAMKKNKGHQLDALANREDHEQVLDVRDFAEH
GSAPLRRWEDALDLPVSLFVLPLFALVNAGVEVSFSSLIETLQHSVGLGIVIGLVIGKFVGISGACWLGLRYKIGSLPEG
INMQHVIGMSLIAGIGFTMSTFIATLGFDSQPEYLQSAKGSILFASLLSAILGLLYLRIIAAKKAPNSASKNGEN
>Mature_475_residues
MNLRIVYLRSLNIQGLTKMTDDKNNLLHRGLDNIQPPFSNFIRAQATASVFLLVATITALWWANSDYSSTYQMLKHMQIG
FFLGDIELQASLKHIINDGLMVIFFFFIGLEIKREVLAGDLAIAENRRMLILCALGGMICPAVIYSLFNWSLDSQIGWGI
PMATDTAFALGALTLVRKHIPISLLAFLVGLAIVDDVGAIVVIALFYTQEISVIFLSISFSLIAFLAIANYAGVLRPIFY
ILIGIAAWWTMLKSGVHPTFAGVAIALTVPARPMLPPDELLSKVKAKITAMKKNKGHQLDALANREDHEQVLDVRDFAEH
GSAPLRRWEDALDLPVSLFVLPLFALVNAGVEVSFSSLIETLQHSVGLGIVIGLVIGKFVGISGACWLGLRYKIGSLPEG
INMQHVIGMSLIAGIGFTMSTFIATLGFDSQPEYLQSAKGSILFASLLSAILGLLYLRIIAAKKAPNSASKNGEN

Specific function: Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons

COG id: COG3004

COG function: function code P; Na+/H+ antiporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaA Na(+)/H(+) (TC 2.A.33) antiporter family

Homologues:

Organism=Escherichia coli, GI1786201, Length=394, Percent_Identity=38.5786802030457, Blast_Score=243, Evalue=3e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NHAA2_PSYIN (A1SRH4)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_941688.1
- ProteinModelPortal:   A1SRH4
- SMR:   A1SRH4
- STRING:   A1SRH4
- GeneID:   4627163
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_0222
- eggNOG:   COG3004
- HOGENOM:   HBG665619
- OMA:   HIINDGL
- PhylomeDB:   A1SRH4
- ProtClustDB:   CLSK773938
- BioCyc:   PING357804:PING_0222-MONOMER
- HAMAP:   MF_01844
- InterPro:   IPR023171
- InterPro:   IPR004670
- TIGRFAMs:   TIGR00773

Pfam domain/function: PF06965 Na_H_antiport_1

EC number: NA

Molecular weight: Translated: 51776; Mature: 51776

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x15b475f8)-; HASH(0x1564073c)-; HASH(0x15be55e0)-; HASH(0x14625a00)-; HASH(0x15a66638)-; HASH(0x15963620)-; HASH(0x148c5918)-; HASH(0x1494f5fc)-; HASH(0x15951fdc)-; HASH(0x14ace95c)-; HASH(0xc0bea48)-; HASH(0x14feb304)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLRIVYLRSLNIQGLTKMTDDKNNLLHRGLDNIQPPFSNFIRAQATASVFLLVATITAL
CCEEEEEEEECCCCCCHHCCCCHHHHHHHCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
WWANSDYSSTYQMLKHMQIGFFLGDIELQASLKHIINDGLMVIFFFFIGLEIKREVLAGD
HCCCCCHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LAIAENRRMLILCALGGMICPAVIYSLFNWSLDSQIGWGIPMATDTAFALGALTLVRKHI
HHEECCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
PISLLAFLVGLAIVDDVGAIVVIALFYTQEISVIFLSISFSLIAFLAIANYAGVLRPIFY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILIGIAAWWTMLKSGVHPTFAGVAIALTVPARPMLPPDELLSKVKAKITAMKKNKGHQLD
HHHHHHHHHHHHHCCCCCCHHCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCHH
ALANREDHEQVLDVRDFAEHGSAPLRRWEDALDLPVSLFVLPLFALVNAGVEVSFSSLIE
HHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEHHHHHH
TLQHSVGLGIVIGLVIGKFVGISGACWLGLRYKIGSLPEGINMQHVIGMSLIAGIGFTMS
HHHHHCCHHHHHHHHHHHHHCCCCHHHEEEEEECCCCCCCCCHHHHHHHHHHHHCCHHHH
TFIATLGFDSQPEYLQSAKGSILFASLLSAILGLLYLRIIAAKKAPNSASKNGEN
HHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCH
>Mature Secondary Structure
MNLRIVYLRSLNIQGLTKMTDDKNNLLHRGLDNIQPPFSNFIRAQATASVFLLVATITAL
CCEEEEEEEECCCCCCHHCCCCHHHHHHHCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
WWANSDYSSTYQMLKHMQIGFFLGDIELQASLKHIINDGLMVIFFFFIGLEIKREVLAGD
HCCCCCHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LAIAENRRMLILCALGGMICPAVIYSLFNWSLDSQIGWGIPMATDTAFALGALTLVRKHI
HHEECCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
PISLLAFLVGLAIVDDVGAIVVIALFYTQEISVIFLSISFSLIAFLAIANYAGVLRPIFY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILIGIAAWWTMLKSGVHPTFAGVAIALTVPARPMLPPDELLSKVKAKITAMKKNKGHQLD
HHHHHHHHHHHHHCCCCCCHHCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCHH
ALANREDHEQVLDVRDFAEHGSAPLRRWEDALDLPVSLFVLPLFALVNAGVEVSFSSLIE
HHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEHHHHHH
TLQHSVGLGIVIGLVIGKFVGISGACWLGLRYKIGSLPEGINMQHVIGMSLIAGIGFTMS
HHHHHCCHHHHHHHHHHHHHCCCCHHHEEEEEECCCCCCCCCHHHHHHHHHHHHCCHHHH
TFIATLGFDSQPEYLQSAKGSILFASLLSAILGLLYLRIIAAKKAPNSASKNGEN
HHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: Na (I) [Cytoplasm] + 2 Proton [Periplasm] = Na (I) [Periplasm] + 2 Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA