Click here to switch to the map view.

The map label for this gene is murB [H]

Identifier: 119944004

GI number: 119944004

Start: 273270

End: 274334

Strand: Direct

Name: murB [H]

Synonym: Ping_0218

Alternate gene names: 119944004

Gene position: 273270-274334 (Clockwise)

Preceding gene: 119944003

Following gene: 119944005

Centisome position: 5.99

GC content: 41.78

Gene sequence:

>1065_bases
ATGATCGAAACTAATACTTCTCTCAAGGCTTTTAATACTTTTTCTATTGATGTTAACGCACAAATATTATTTCATTTTAA
CAATCTTAAACAGATTCCCGAACTGTTAGACTTGGTGAAAACTACCCGCGCGGACAATAAGCCTGTCTTAATTTTAGGCG
GCGGCAGTAATATATTGTTCTGTGAAGATTTTACAGGCTTAGTGATTAAGGTTGATTTGTCGGGCGTTGATATAACTGAA
TCTGCTGATAGTTATTTATTAAAAGTCGCAGCGGGGGAGAACTGGCATAAGTTAGTCGCCGACTGTATTGATAAAGGCAT
CAATGGATTGGAAAACTTAGCCTTGATCCCAGGGGTTGTTGGCGCCGCACCGGTGCAAAATATCGGTGCCTATGGCACAG
AATTTAAAGATTTTTGTGAGTCGGTTGAATATCTTGATTTGAATTCGGGCGCATTACATTGTTTGAGCGCCAAAGAGTGT
TTATTTGCCTACCGTGACAGTATTTTTAAAACACTAAAAATGCAGGATGCATTAATCACCAGAGTAACCCTTAAATTGAC
TAAGGACTGGCAAGCGCAGAATCAATATGGCCGTTTGAAAAATGCAGCCGAATCGGCTCAAAAACTGAGTGCAAAACAGA
TTTTTACCTCTGTCTGTCAAATTCGCAGTGAAAAATTACCCGATCCCGCCAAGTTGGGTAATGCAGGCAGTTTTTTTAAA
AATCCATTAGTAACAGCGCCGCAAGCAGAGCAGTTACTGGCTTTATATCCGCATATGCCCCATTACCCGCAGCCGGATGG
GCAAGTAAAATTAGCGGCAGGGTGGCTTATCGAGCAGGCCAATCTAAAAGGCGCTCATATTGGTGGTGCGGCTGTGCATC
AGCAGCAAGCCTTGGTATTAATTAACCAAAACAATGCCTGCGCTGCAGATGTTATTCAATTGGCAAATTTAGTGCGGACA
ACAGTAAAAGAAAAATTTAATATAAACTTAGAACACGAAGTGCGCTTTATCTGCGCGATGGGCGAGAGCAACTTAACAGA
GGCGGTTAACAAATGTCGGAACTGA

Upstream 100 bases:

>100_bases
GTAAACTCAGTGGAGATACCTAAACGACTTGAAGATGCCGATGCCTGCTTCTTTCATAATTTGTATCTTCAGGGTTTACA
CTTCACATAGCGGGATAATG

Downstream 100 bases:

>100_bases
ATGAACAGGGTAATATTCTTTTAACGTTACTTGCCGATGGAGAGTTTCATTCGGGTGAAAAAATAGGCGAACTTCTAGGT
GTTTCGCGTACCTCGGTTAA

Product: UDP-N-acetylenolpyruvoylglucosamine reductase

Products: NA

Alternate protein names: UDP-N-acetylmuramate dehydrogenase [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MIETNTSLKAFNTFSIDVNAQILFHFNNLKQIPELLDLVKTTRADNKPVLILGGGSNILFCEDFTGLVIKVDLSGVDITE
SADSYLLKVAAGENWHKLVADCIDKGINGLENLALIPGVVGAAPVQNIGAYGTEFKDFCESVEYLDLNSGALHCLSAKEC
LFAYRDSIFKTLKMQDALITRVTLKLTKDWQAQNQYGRLKNAAESAQKLSAKQIFTSVCQIRSEKLPDPAKLGNAGSFFK
NPLVTAPQAEQLLALYPHMPHYPQPDGQVKLAAGWLIEQANLKGAHIGGAAVHQQQALVLINQNNACAADVIQLANLVRT
TVKEKFNINLEHEVRFICAMGESNLTEAVNKCRN

Sequences:

>Translated_354_residues
MIETNTSLKAFNTFSIDVNAQILFHFNNLKQIPELLDLVKTTRADNKPVLILGGGSNILFCEDFTGLVIKVDLSGVDITE
SADSYLLKVAAGENWHKLVADCIDKGINGLENLALIPGVVGAAPVQNIGAYGTEFKDFCESVEYLDLNSGALHCLSAKEC
LFAYRDSIFKTLKMQDALITRVTLKLTKDWQAQNQYGRLKNAAESAQKLSAKQIFTSVCQIRSEKLPDPAKLGNAGSFFK
NPLVTAPQAEQLLALYPHMPHYPQPDGQVKLAAGWLIEQANLKGAHIGGAAVHQQQALVLINQNNACAADVIQLANLVRT
TVKEKFNINLEHEVRFICAMGESNLTEAVNKCRN
>Mature_354_residues
MIETNTSLKAFNTFSIDVNAQILFHFNNLKQIPELLDLVKTTRADNKPVLILGGGSNILFCEDFTGLVIKVDLSGVDITE
SADSYLLKVAAGENWHKLVADCIDKGINGLENLALIPGVVGAAPVQNIGAYGTEFKDFCESVEYLDLNSGALHCLSAKEC
LFAYRDSIFKTLKMQDALITRVTLKLTKDWQAQNQYGRLKNAAESAQKLSAKQIFTSVCQIRSEKLPDPAKLGNAGSFFK
NPLVTAPQAEQLLALYPHMPHYPQPDGQVKLAAGWLIEQANLKGAHIGGAAVHQQQALVLINQNNACAADVIQLANLVRT
TVKEKFNINLEHEVRFICAMGESNLTEAVNKCRN

Specific function: Cell wall formation [H]

COG id: COG0812

COG function: function code M; UDP-N-acetylmuramate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Escherichia coli, GI1790407, Length=346, Percent_Identity=51.7341040462428, Blast_Score=347, Evalue=9e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016169
- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR003170
- InterPro:   IPR011601
- InterPro:   IPR006094 [H]

Pfam domain/function: PF01565 FAD_binding_4; PF02873 MurB_C [H]

EC number: =1.1.1.158 [H]

Molecular weight: Translated: 38739; Mature: 38739

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIETNTSLKAFNTFSIDVNAQILFHFNNLKQIPELLDLVKTTRADNKPVLILGGGSNILF
CCCCCCCCEEEEEEEEEECEEEEEEECCHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEE
CEDFTGLVIKVDLSGVDITESADSYLLKVAAGENWHKLVADCIDKGINGLENLALIPGVV
EECCCCEEEEEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHCCCC
GAAPVQNIGAYGTEFKDFCESVEYLDLNSGALHCLSAKECLFAYRDSIFKTLKMQDALIT
CCCCHHHHCCCCCHHHHHHCCCCEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
RVTLKLTKDWQAQNQYGRLKNAAESAQKLSAKQIFTSVCQIRSEKLPDPAKLGNAGSFFK
HEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHC
NPLVTAPQAEQLLALYPHMPHYPQPDGQVKLAAGWLIEQANLKGAHIGGAAVHQQQALVL
CCCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCHHHCCCCEEEE
INQNNACAADVIQLANLVRTTVKEKFNINLEHEVRFICAMGESNLTEAVNKCRN
EECCCCHHHHHHHHHHHHHHHHHHHHCCCEEHEEEEEEECCCCHHHHHHHHHCC
>Mature Secondary Structure
MIETNTSLKAFNTFSIDVNAQILFHFNNLKQIPELLDLVKTTRADNKPVLILGGGSNILF
CCCCCCCCEEEEEEEEEECEEEEEEECCHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEE
CEDFTGLVIKVDLSGVDITESADSYLLKVAAGENWHKLVADCIDKGINGLENLALIPGVV
EECCCCEEEEEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHCCCC
GAAPVQNIGAYGTEFKDFCESVEYLDLNSGALHCLSAKECLFAYRDSIFKTLKMQDALIT
CCCCHHHHCCCCCHHHHHHCCCCEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
RVTLKLTKDWQAQNQYGRLKNAAESAQKLSAKQIFTSVCQIRSEKLPDPAKLGNAGSFFK
HEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHC
NPLVTAPQAEQLLALYPHMPHYPQPDGQVKLAAGWLIEQANLKGAHIGGAAVHQQQALVL
CCCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCHHHCCCCEEEE
INQNNACAADVIQLANLVRTTVKEKFNINLEHEVRFICAMGESNLTEAVNKCRN
EECCCCHHHHHHHHHHHHHHHHHHHHCCCEEHEEEEEEECCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]