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The map label for this gene is cca

Identifier: 119943963

GI number: 119943963

Start: 223829

End: 225058

Strand: Reverse

Name: cca

Synonym: Ping_0176

Alternate gene names: 119943963

Gene position: 225058-223829 (Counterclockwise)

Preceding gene: 119943966

Following gene: 119943962

Centisome position: 4.94

GC content: 43.9

Gene sequence:

>1230_bases
ATGCAAATATTTTTAGTTGGAGGCGCAGTTCGTGATCAATTATTACAACTTAAGGTTAAAGATCGAGATTATGTAGTGAT
CGCAGCCACCCCGGATAAACTATTAAAGTTAGGTTTTCAGCAAGTAGGTAAAGATTTTCCCGTTTTTATCCATCCTCAAA
CGGGTGATGAATATGCCCTCGCACGCACTGAGCGAAAAAAAGGTTCCGGTCATAATGGATTTGAATGTTACGCTGGCCTG
GATGTCACTCTCACGGAAGACTTAAAACGCCGAGATTTAACCATTAATGCCATTGCACAATCACCACAGGGAGAGTTGAT
TGATCCCTACCACGGGCTGCAGGATATACAGACCAAAACCCTGCGTCATATATCGACAGCATTTAGCGAAGATCCGTTGC
GCGTTTTACGCGTTGCACGCTTCGCTGCGCGTTTTTACGATTTAGGCTTCAAAATTGCCCCGGAAACGCTCGATCTGATG
CGCAGCTTAAGCAGTAGTGGTGAGTTAAATCATCTGACCGCAGAAAGGGTTTGGCAGGAAACCGCAAATGCGTTAAAAAC
CAATAACCCGCAGATCTATTTCCAGGTGTTACGCGACTGTGGTGCTCTGCCGCTTTTATTCCCCGAAATTGAAGCATTAT
TTGGTGTTCCTGCACCTAAAAAATGGCATCCTGAAATTGATACTGGCATACATACTTTAATGGTTGTAGAACAATCAGTA
AAATTATCCGATTCACTTGCGTTTCGTTTTGCCTGCCTGGTACACGACCTTGGTAAGGCGCTAACACCCAAGGAACTCTG
GCCCAGCCATAAAGGTCATGGGAAATTGGGTTTGCAGCTGATTAACAACTTATGTGAACGTCTTAAAATACCCAATGAAT
GCAGAGAATTGGCCTGTTTAGTCAGTGAGCACCATACGCTTATCCATAAGGGGTTGGAGTTAGAAGCAGACAGCCTAATC
ACATTAATGGATCAAAACGATGCATGGCGTAAGCCTGAACGGTTTTCGCAAATGTTACAGTGCTGCGTTGCCGACTCTAA
GGGCCGAACCGGTTTTGAAGAGAAAGACTACCCCAGTGCAGATTATATTTGGCGTGCTTTTCAAGTGGCACAGTTAGTTG
ATGTACAACCAATTATTAAGCAAGGATATCAAGGTGCAGAGATTAAAGCTCAGTTAAAACATGCACGTATTCGATCAGTC
GAACAGTATAAACAGAAGCACAGCGTATAA

Upstream 100 bases:

>100_bases
TAAAGAGAAAAAATCTTGATACATAACAAAATCCAAAGAAAATAGTGACAAGAGAAGTTATTGCATATAATAACTAACTA
AGACATAAAGAGGAAAAACA

Downstream 100 bases:

>100_bases
TCGACTGACTTATTACGATCGCAACTTAATTTAAAGAGGCGGAAAATGTCTAATTCACTGACTATTCTCCCATTGCATAA
CTTGGCTATTGCCTTTATTC

Product: fused tRNA nucleotidyl transferase and 2'3'-cyclic phosphodiesterase/2'nucleotidase/phosphatase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MQIFLVGGAVRDQLLQLKVKDRDYVVIAATPDKLLKLGFQQVGKDFPVFIHPQTGDEYALARTERKKGSGHNGFECYAGL
DVTLTEDLKRRDLTINAIAQSPQGELIDPYHGLQDIQTKTLRHISTAFSEDPLRVLRVARFAARFYDLGFKIAPETLDLM
RSLSSSGELNHLTAERVWQETANALKTNNPQIYFQVLRDCGALPLLFPEIEALFGVPAPKKWHPEIDTGIHTLMVVEQSV
KLSDSLAFRFACLVHDLGKALTPKELWPSHKGHGKLGLQLINNLCERLKIPNECRELACLVSEHHTLIHKGLELEADSLI
TLMDQNDAWRKPERFSQMLQCCVADSKGRTGFEEKDYPSADYIWRAFQVAQLVDVQPIIKQGYQGAEIKAQLKHARIRSV
EQYKQKHSV

Sequences:

>Translated_409_residues
MQIFLVGGAVRDQLLQLKVKDRDYVVIAATPDKLLKLGFQQVGKDFPVFIHPQTGDEYALARTERKKGSGHNGFECYAGL
DVTLTEDLKRRDLTINAIAQSPQGELIDPYHGLQDIQTKTLRHISTAFSEDPLRVLRVARFAARFYDLGFKIAPETLDLM
RSLSSSGELNHLTAERVWQETANALKTNNPQIYFQVLRDCGALPLLFPEIEALFGVPAPKKWHPEIDTGIHTLMVVEQSV
KLSDSLAFRFACLVHDLGKALTPKELWPSHKGHGKLGLQLINNLCERLKIPNECRELACLVSEHHTLIHKGLELEADSLI
TLMDQNDAWRKPERFSQMLQCCVADSKGRTGFEEKDYPSADYIWRAFQVAQLVDVQPIIKQGYQGAEIKAQLKHARIRSV
EQYKQKHSV
>Mature_409_residues
MQIFLVGGAVRDQLLQLKVKDRDYVVIAATPDKLLKLGFQQVGKDFPVFIHPQTGDEYALARTERKKGSGHNGFECYAGL
DVTLTEDLKRRDLTINAIAQSPQGELIDPYHGLQDIQTKTLRHISTAFSEDPLRVLRVARFAARFYDLGFKIAPETLDLM
RSLSSSGELNHLTAERVWQETANALKTNNPQIYFQVLRDCGALPLLFPEIEALFGVPAPKKWHPEIDTGIHTLMVVEQSV
KLSDSLAFRFACLVHDLGKALTPKELWPSHKGHGKLGLQLINNLCERLKIPNECRELACLVSEHHTLIHKGLELEADSLI
TLMDQNDAWRKPERFSQMLQCCVADSKGRTGFEEKDYPSADYIWRAFQVAQLVDVQPIIKQGYQGAEIKAQLKHARIRSV
EQYKQKHSV

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1789436, Length=406, Percent_Identity=60.5911330049261, Blast_Score=528, Evalue=1e-151,
Organism=Escherichia coli, GI87081691, Length=230, Percent_Identity=31.7391304347826, Blast_Score=82, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_PSYIN (A1SRC9)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_941643.1
- ProteinModelPortal:   A1SRC9
- SMR:   A1SRC9
- STRING:   A1SRC9
- GeneID:   4626821
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_0176
- eggNOG:   COG0617
- HOGENOM:   HBG635063
- OMA:   GHGQKGL
- PhylomeDB:   A1SRC9
- BioCyc:   PING357804:PING_0176-MONOMER
- HAMAP:   MF_01261
- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646
- PIRSF:   PIRSF000813
- SMART:   SM00471

Pfam domain/function: PF01743 PolyA_pol

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 46271; Mature: 46271

Theoretical pI: Translated: 7.62; Mature: 7.62

Prosite motif: NA

Important sites: BINDING 8-8 BINDING 11-11 BINDING 91-91 BINDING 137-137 BINDING 140-140

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIFLVGGAVRDQLLQLKVKDRDYVVIAATPDKLLKLGFQQVGKDFPVFIHPQTGDEYAL
CEEEEECHHHHHHHHHEEECCCCEEEEEECHHHHHHHHHHHHCCCCCEEEECCCCCCHHH
ARTERKKGSGHNGFECYAGLDVTLTEDLKRRDLTINAIAQSPQGELIDPYHGLQDIQTKT
HHHHHHCCCCCCCCEEEECCCEEEHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHH
LRHISTAFSEDPLRVLRVARFAARFYDLGFKIAPETLDLMRSLSSSGELNHLTAERVWQE
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHCCCCCCHHHHHHHHHH
TANALKTNNPQIYFQVLRDCGALPLLFPEIEALFGVPAPKKWHPEIDTGIHTLMVVEQSV
HHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
KLSDSLAFRFACLVHDLGKALTPKELWPSHKGHGKLGLQLINNLCERLKIPNECRELACL
HHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
VSEHHTLIHKGLELEADSLITLMDQNDAWRKPERFSQMLQCCVADSKGRTGFEEKDYPSA
HHHHHHHHHCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCH
DYIWRAFQVAQLVDVQPIIKQGYQGAEIKAQLKHARIRSVEQYKQKHSV
HHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQIFLVGGAVRDQLLQLKVKDRDYVVIAATPDKLLKLGFQQVGKDFPVFIHPQTGDEYAL
CEEEEECHHHHHHHHHEEECCCCEEEEEECHHHHHHHHHHHHCCCCCEEEECCCCCCHHH
ARTERKKGSGHNGFECYAGLDVTLTEDLKRRDLTINAIAQSPQGELIDPYHGLQDIQTKT
HHHHHHCCCCCCCCEEEECCCEEEHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHH
LRHISTAFSEDPLRVLRVARFAARFYDLGFKIAPETLDLMRSLSSSGELNHLTAERVWQE
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHCCCCCCHHHHHHHHHH
TANALKTNNPQIYFQVLRDCGALPLLFPEIEALFGVPAPKKWHPEIDTGIHTLMVVEQSV
HHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
KLSDSLAFRFACLVHDLGKALTPKELWPSHKGHGKLGLQLINNLCERLKIPNECRELACL
HHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
VSEHHTLIHKGLELEADSLITLMDQNDAWRKPERFSQMLQCCVADSKGRTGFEEKDYPSA
HHHHHHHHHCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCH
DYIWRAFQVAQLVDVQPIIKQGYQGAEIKAQLKHARIRSVEQYKQKHSV
HHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA