Click here to switch to the map view.

The map label for this gene is glgE [H]

Identifier: 119869964

GI number: 119869964

Start: 4140473

End: 4142560

Strand: Direct

Name: glgE [H]

Synonym: Mkms_3934

Alternate gene names: 119869964

Gene position: 4140473-4142560 (Clockwise)

Preceding gene: 119869960

Following gene: 119869965

Centisome position: 72.17

GC content: 67.67

Gene sequence:

>2088_bases
GTGGCCGGCCGTATCGGAATCGATGACGTCGCGCCCGTGGTATCCGGCGGCAGGTATCCGGCCAAAGCGGTGGTGGGTGA
GGTGGTGCCGGTGAGCGCCACGGTGTGGCGCGAGGGCCACGAAGCCGTCTCCGCGACGCTGGTGGTGCGCTATCACGGCA
CGTCCTATCCCGAACTCGCCGACGCTCCGGTCGGCCGGGTGAGCACCACCGAGGCCGTGCCGATCGACGAGGTCGTCAGC
CCTTCGCCGCGGAAGAAGCCGCAGGCGCTGCCGATGAGCCTGGGCCGCACCCCCGATGTCTTCCACGGGCACTTCGTACC
GGACGCGGTCGGGTTGTGGACCTACCGTGTCGACGGGTGGGGCGATCCGATCGCCAGCTGGCGCCACAACGTGATCGCCA
AACTCGACGCCGGCCAGAGCGAGTCCGAACTGGACAACGATCTGCTCGTGGGCGCGCGGCTGCTGGAGCGGGCCGCCACC
GGTGTCCCCCGTCAGGACCGCTACCCGCTGGCGCAGGCCGTCGAACGACTGCGCGAACCCGGCGATCCGTTCTACCGGGC
GGGGGCGGCGCTGGCGGCCGACGTCACCGCACTGCTCGACCAGTATCCGCTGCGCGAATTGGTCACCCGCGGTGAGCAAT
ACGGCGTCTGGGTGGACCGCCCGCTGGCCCGCTTCGGCTCCTGGTACGAGTTCTTCCCCCGCTCCACCGGCGGCTGGGAC
AGCCAGGGCCACCCCGTGCACGGCACGTTCGCCACCGCCACCAAGGCGCTGCCCCGGGTCGCCCGGATGGGCTTCGACGT
GGTCTACCTCCCGCCGATCCACCCGATCGGCAAGGTGCACCGCAAGGGCCCGAACAACAGCGTGACCGCGGGCCCGAACG
ACGTGGGGTCACCGTGGGCGATCGGCAGCGACGAGGGCGGCCACGACGCCGTGCACCCGGAGTTGGGCACCATCGAGGAC
TTCGACGACTTCGTCGCCGCGGCCCGTGACGAAGGCCTCGAGGTGGCGCTGGACCTGGCGCTGCAGTGTGCGCCGGACCA
TCCGTGGGCGCGCGAGCATCCGGAGTGGTTCACCGTGCTGCCCGACGGCACCATCGCCTACGCGGAGAACCCGCCGAAGA
AGTACCAGGACATCTATCCACTGAACTTCGACAACGACCCGACGGGGTTGTACATGGAGGTGCTGCGGGTGGTCCGGTTC
TGGATCTCCCACGGCGTCAAGGTCTTTCGCGTGGACAACCCACACACCAAACCGCCCAACTTCTGGGCGTGGCTGATCGG
GGAGATCAAGAACGAGGACCCCGACGTGCTGTTCCTGGCCGAGGCGTTCACCCGGCCGGCCCGCCTGTACGGGCTGGCGA
AACTCGGCTACACGCAGTCGTACACGTACTTCACCTGGCGGACGTCGAAACACGAGTTGACCGAGTTCGGCAGGCAGATC
GCCGAACACGCCGACTATGCGCGGCCCAACCTGTTCGTCAACACCCCCGACATCCTGCACGAGAGCCTCCAGCACGGCGG
CCCCGGCATGTTCGCGATCCGCGCGGTGCTGGCGTCCACGATGAGTTCGGTGTGGGGGGTCTACTCCGGTTACGAACTGT
TCGAACACCGACCGGTGCGGGAAGGCAGCGAGGAGTACCTCAACTCCGAGAAGTACGAACTGCGTCCGCGCGACTTCGAC
GCCGCCCTGGCCGACGGGGAGTCACTGGAACCGTTCATCACCCGGCTCAACGAGATTCGGCGGGTGCATCCGGCGCTGCA
CGAACTGCGGACCATCACGTTCCACTACCCCGACAACGACGCCGTCCTGGCCTACAGCAAGTTCGACCCGGCCACCGGTG
ACCAGGTCCTCGTCGTAGTCACGCTCAACCCGTTCGGGCCCGAAGAGGCCACAGTGTGGTTGGACATGCCCGCATTGGGA
ATGGAGCCATACGACAGGTTCTGGGTCCGCGACGAGATCACCGGAGAGGAATACCAGTGGGGGCAGTCGAACTACGTGCG
CATCGAACCGGCGAAGGCGGTCGCGCACGTGCTCAACATGCCCCAGGTGCCCGCCGACCAACGCCTCAACCTACTGCGTA
GGGAGTGA

Upstream 100 bases:

>100_bases
GCGCGGAGAAGGTCTACGACAACCGACCGAGACAGAACCAACCGACAGAGTGGCCACATCGACGATGCTGGTGGTCGCGC
CGATTCGAAGAGGAGTGGTG

Downstream 100 bases:

>100_bases
ACACACGCATGGCCAAGACAAAAGGACTGCCGAAAGACACCGCGGTGACCCCCAGCCCGCATCTGCGGCCGCACACCGCC
GACCTGAACCGGTTGCTCGC

Product: alpha amylase catalytic subunit

Products: D-Glucose 6-phosphate; D-Glucose [C]

Alternate protein names: NA

Number of amino acids: Translated: 695; Mature: 694

Protein sequence:

>695_residues
MAGRIGIDDVAPVVSGGRYPAKAVVGEVVPVSATVWREGHEAVSATLVVRYHGTSYPELADAPVGRVSTTEAVPIDEVVS
PSPRKKPQALPMSLGRTPDVFHGHFVPDAVGLWTYRVDGWGDPIASWRHNVIAKLDAGQSESELDNDLLVGARLLERAAT
GVPRQDRYPLAQAVERLREPGDPFYRAGAALAADVTALLDQYPLRELVTRGEQYGVWVDRPLARFGSWYEFFPRSTGGWD
SQGHPVHGTFATATKALPRVARMGFDVVYLPPIHPIGKVHRKGPNNSVTAGPNDVGSPWAIGSDEGGHDAVHPELGTIED
FDDFVAAARDEGLEVALDLALQCAPDHPWAREHPEWFTVLPDGTIAYAENPPKKYQDIYPLNFDNDPTGLYMEVLRVVRF
WISHGVKVFRVDNPHTKPPNFWAWLIGEIKNEDPDVLFLAEAFTRPARLYGLAKLGYTQSYTYFTWRTSKHELTEFGRQI
AEHADYARPNLFVNTPDILHESLQHGGPGMFAIRAVLASTMSSVWGVYSGYELFEHRPVREGSEEYLNSEKYELRPRDFD
AALADGESLEPFITRLNEIRRVHPALHELRTITFHYPDNDAVLAYSKFDPATGDQVLVVVTLNPFGPEEATVWLDMPALG
MEPYDRFWVRDEITGEEYQWGQSNYVRIEPAKAVAHVLNMPQVPADQRLNLLRRE

Sequences:

>Translated_695_residues
MAGRIGIDDVAPVVSGGRYPAKAVVGEVVPVSATVWREGHEAVSATLVVRYHGTSYPELADAPVGRVSTTEAVPIDEVVS
PSPRKKPQALPMSLGRTPDVFHGHFVPDAVGLWTYRVDGWGDPIASWRHNVIAKLDAGQSESELDNDLLVGARLLERAAT
GVPRQDRYPLAQAVERLREPGDPFYRAGAALAADVTALLDQYPLRELVTRGEQYGVWVDRPLARFGSWYEFFPRSTGGWD
SQGHPVHGTFATATKALPRVARMGFDVVYLPPIHPIGKVHRKGPNNSVTAGPNDVGSPWAIGSDEGGHDAVHPELGTIED
FDDFVAAARDEGLEVALDLALQCAPDHPWAREHPEWFTVLPDGTIAYAENPPKKYQDIYPLNFDNDPTGLYMEVLRVVRF
WISHGVKVFRVDNPHTKPPNFWAWLIGEIKNEDPDVLFLAEAFTRPARLYGLAKLGYTQSYTYFTWRTSKHELTEFGRQI
AEHADYARPNLFVNTPDILHESLQHGGPGMFAIRAVLASTMSSVWGVYSGYELFEHRPVREGSEEYLNSEKYELRPRDFD
AALADGESLEPFITRLNEIRRVHPALHELRTITFHYPDNDAVLAYSKFDPATGDQVLVVVTLNPFGPEEATVWLDMPALG
MEPYDRFWVRDEITGEEYQWGQSNYVRIEPAKAVAHVLNMPQVPADQRLNLLRRE
>Mature_694_residues
AGRIGIDDVAPVVSGGRYPAKAVVGEVVPVSATVWREGHEAVSATLVVRYHGTSYPELADAPVGRVSTTEAVPIDEVVSP
SPRKKPQALPMSLGRTPDVFHGHFVPDAVGLWTYRVDGWGDPIASWRHNVIAKLDAGQSESELDNDLLVGARLLERAATG
VPRQDRYPLAQAVERLREPGDPFYRAGAALAADVTALLDQYPLRELVTRGEQYGVWVDRPLARFGSWYEFFPRSTGGWDS
QGHPVHGTFATATKALPRVARMGFDVVYLPPIHPIGKVHRKGPNNSVTAGPNDVGSPWAIGSDEGGHDAVHPELGTIEDF
DDFVAAARDEGLEVALDLALQCAPDHPWAREHPEWFTVLPDGTIAYAENPPKKYQDIYPLNFDNDPTGLYMEVLRVVRFW
ISHGVKVFRVDNPHTKPPNFWAWLIGEIKNEDPDVLFLAEAFTRPARLYGLAKLGYTQSYTYFTWRTSKHELTEFGRQIA
EHADYARPNLFVNTPDILHESLQHGGPGMFAIRAVLASTMSSVWGVYSGYELFEHRPVREGSEEYLNSEKYELRPRDFDA
ALADGESLEPFITRLNEIRRVHPALHELRTITFHYPDNDAVLAYSKFDPATGDQVLVVVTLNPFGPEEATVWLDMPALGM
EPYDRFWVRDEITGEEYQWGQSNYVRIEPAKAVAHVLNMPQVPADQRLNLLRRE

Specific function: Could be involved in glycogen catabolism [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family. GlgE subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021828
- InterPro:   IPR013780
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF11896 DUF3416 [H]

EC number: 3.2.1.93 [C]

Molecular weight: Translated: 77688; Mature: 77557

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGRIGIDDVAPVVSGGRYPAKAVVGEVVPVSATVWREGHEAVSATLVVRYHGTSYPELA
CCCCCCCHHHHHHHCCCCCCHHHHHCCCCCCCHHHHHCCHHHEEEEEEEEECCCCCCCHH
DAPVGRVSTTEAVPIDEVVSPSPRKKPQALPMSLGRTPDVFHGHFVPDAVGLWTYRVDGW
CCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCC
GDPIASWRHNVIAKLDAGQSESELDNDLLVGARLLERAATGVPRQDRYPLAQAVERLREP
CCHHHHHHCCEEEEECCCCCHHHHCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCC
GDPFYRAGAALAADVTALLDQYPLRELVTRGEQYGVWVDRPLARFGSWYEFFPRSTGGWD
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCEEECCHHHHHCCHHHHCCCCCCCCC
SQGHPVHGTFATATKALPRVARMGFDVVYLPPIHPIGKVHRKGPNNSVTAGPNDVGSPWA
CCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCCC
IGSDEGGHDAVHPELGTIEDFDDFVAAARDEGLEVALDLALQCAPDHPWAREHPEWFTVL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEHEEECCCCCCCCCCCCEEEEC
PDGTIAYAENPPKKYQDIYPLNFDNDPTGLYMEVLRVVRFWISHGVKVFRVDNPHTKPPN
CCCEEEECCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCC
FWAWLIGEIKNEDPDVLFLAEAFTRPARLYGLAKLGYTQSYTYFTWRTSKHELTEFGRQI
CCEEEEHHHCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHH
AEHADYARPNLFVNTPDILHESLQHGGPGMFAIRAVLASTMSSVWGVYSGYELFEHRPVR
HHHCCCCCCCEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCC
EGSEEYLNSEKYELRPRDFDAALADGESLEPFITRLNEIRRVHPALHELRTITFHYPDND
CCHHHHHCCCCEECCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHCEEEEEEECCCC
AVLAYSKFDPATGDQVLVVVTLNPFGPEEATVWLDMPALGMEPYDRFWVRDEITGEEYQW
CEEEEECCCCCCCCCEEEEEEECCCCCCCCEEEEECCCCCCCHHHHEEEEECCCCCCCCC
GQSNYVRIEPAKAVAHVLNMPQVPADQRLNLLRRE
CCCCEEEEEHHHHHHHHHCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
AGRIGIDDVAPVVSGGRYPAKAVVGEVVPVSATVWREGHEAVSATLVVRYHGTSYPELA
CCCCCCHHHHHHHCCCCCCHHHHHCCCCCCCHHHHHCCHHHEEEEEEEEECCCCCCCHH
DAPVGRVSTTEAVPIDEVVSPSPRKKPQALPMSLGRTPDVFHGHFVPDAVGLWTYRVDGW
CCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCC
GDPIASWRHNVIAKLDAGQSESELDNDLLVGARLLERAATGVPRQDRYPLAQAVERLREP
CCHHHHHHCCEEEEECCCCCHHHHCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCC
GDPFYRAGAALAADVTALLDQYPLRELVTRGEQYGVWVDRPLARFGSWYEFFPRSTGGWD
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCEEECCHHHHHCCHHHHCCCCCCCCC
SQGHPVHGTFATATKALPRVARMGFDVVYLPPIHPIGKVHRKGPNNSVTAGPNDVGSPWA
CCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCCC
IGSDEGGHDAVHPELGTIEDFDDFVAAARDEGLEVALDLALQCAPDHPWAREHPEWFTVL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEHEEECCCCCCCCCCCCEEEEC
PDGTIAYAENPPKKYQDIYPLNFDNDPTGLYMEVLRVVRFWISHGVKVFRVDNPHTKPPN
CCCEEEECCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCC
FWAWLIGEIKNEDPDVLFLAEAFTRPARLYGLAKLGYTQSYTYFTWRTSKHELTEFGRQI
CCEEEEHHHCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHH
AEHADYARPNLFVNTPDILHESLQHGGPGMFAIRAVLASTMSSVWGVYSGYELFEHRPVR
HHHCCCCCCCEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCC
EGSEEYLNSEKYELRPRDFDAALADGESLEPFITRLNEIRRVHPALHELRTITFHYPDND
CCHHHHHCCCCEECCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHCEEEEEEECCCC
AVLAYSKFDPATGDQVLVVVTLNPFGPEEATVWLDMPALGMEPYDRFWVRDEITGEEYQW
CEEEEECCCCCCCCCEEEEEEECCCCCCCCEEEEECCCCCCCHHHHEEEEECCCCCCCCC
GQSNYVRIEPAKAVAHVLNMPQVPADQRLNLLRRE
CCCCEEEEEHHHHHHHHHCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; alpha,alpha'-Trehalose 6-phosphate [C]

Specific reaction: H2O + alpha,alpha'-Trehalose 6-phosphate --> D-Glucose 6-phosphate + D-Glucose [C]

General reaction: O-Glycosyl bond hydrolysis [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10542168 [H]