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The map label for this gene is echA8 [H]

Identifier: 119869645

GI number: 119869645

Start: 3801012

End: 3801833

Strand: Direct

Name: echA8 [H]

Synonym: Mkms_3613

Alternate gene names: 119869645

Gene position: 3801012-3801833 (Clockwise)

Preceding gene: 119869644

Following gene: 119869646

Centisome position: 66.25

GC content: 69.46

Gene sequence:

>822_bases
ATGTCAGAGCACGACGCCCCACGCCCACCGGACGGTGACTGGTTGGGCACGCCCTATCTGCGGTTCACCCGCGAGGGGGC
GTTCGGGGTGTGCACGCTCGACCGCCCCGAGGCGCGCAACGCGATGACCCCGGCGATGTACTTCGGCATCAAGTACGCGG
TCAACCATGTGGGCACCGATCCCGACCTCGCGGGTCTGTTGATCACCGGGACCGGCGACGTGTTCGCGCCTGGCGGCGAC
ATGGGCGGCGGCGGGGCGGACAACTGGTTGACCTTCGGATCGGCGCTGGGTATGGACGCGTTGCCGTTCGACACGCTGCG
CCAGTCCGCGAAACCCGTTGTGGCGGCGGTCAACGGGCTCTGTCAGGGCGGCGGCATGCAGATCGCGCTGTGCAGCGACA
TGGCCGTCGTCAGTGATCGCGCCACGTTCCGGGTGCCCGAGCTCTACCGCGGGATCGCCGACACCTACTACAGCCACATG
CTGGCGCGGCTGATCGGTCCCGTCCGCACCCGGGACCTGATGTTCACCGGCCGCACCCTGTCGGCGGCCGAGGCCCTGGA
CTGGGGGCTGGTCGCGCGGGTGGTGCCCCACGACGACCTCCACGACGCCGCCCGGGAGGTGCTCGCGCAGTGCTGCCGCA
CCGCACCGCATGCGCGCTCGGTCATCAAGTCGAGCCTCGACAACTACATCGGCCTCTACGACCGCATCGGGATGCAGTCG
AGCCTGGGCGCACCCGAGTCGATCGAGGGGTTCATGGCCTTCAAGGAGCGGCGCTCACCCGACTGGGTGCACCCTGAGCT
GCGGATCGACGGGCGGTTGTGA

Upstream 100 bases:

>100_bases
CGTGCGTCGCGGGCGCGCTGGCCACCCTGGTACCCGGCGCGGGTGACTGCGCTCCCACCGCCGAAGCGATCGACGACACA
TTGAGATGACAGGAGAAGCG

Downstream 100 bases:

>100_bases
CCGCGGCATAGCTCAACTGGTTGAACTCGGGACCAATGGCCCTGGTGTGCCGGCCGAACGGAATGCGACGTTCGGTGCGT
GGAGCACCTGAAGACCCTGG

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MSEHDAPRPPDGDWLGTPYLRFTREGAFGVCTLDRPEARNAMTPAMYFGIKYAVNHVGTDPDLAGLLITGTGDVFAPGGD
MGGGGADNWLTFGSALGMDALPFDTLRQSAKPVVAAVNGLCQGGGMQIALCSDMAVVSDRATFRVPELYRGIADTYYSHM
LARLIGPVRTRDLMFTGRTLSAAEALDWGLVARVVPHDDLHDAAREVLAQCCRTAPHARSVIKSSLDNYIGLYDRIGMQS
SLGAPESIEGFMAFKERRSPDWVHPELRIDGRL

Sequences:

>Translated_273_residues
MSEHDAPRPPDGDWLGTPYLRFTREGAFGVCTLDRPEARNAMTPAMYFGIKYAVNHVGTDPDLAGLLITGTGDVFAPGGD
MGGGGADNWLTFGSALGMDALPFDTLRQSAKPVVAAVNGLCQGGGMQIALCSDMAVVSDRATFRVPELYRGIADTYYSHM
LARLIGPVRTRDLMFTGRTLSAAEALDWGLVARVVPHDDLHDAAREVLAQCCRTAPHARSVIKSSLDNYIGLYDRIGMQS
SLGAPESIEGFMAFKERRSPDWVHPELRIDGRL
>Mature_272_residues
SEHDAPRPPDGDWLGTPYLRFTREGAFGVCTLDRPEARNAMTPAMYFGIKYAVNHVGTDPDLAGLLITGTGDVFAPGGDM
GGGGADNWLTFGSALGMDALPFDTLRQSAKPVVAAVNGLCQGGGMQIALCSDMAVVSDRATFRVPELYRGIADTYYSHML
ARLIGPVRTRDLMFTGRTLSAAEALDWGLVARVVPHDDLHDAAREVLAQCCRTAPHARSVIKSSLDNYIGLYDRIGMQSS
LGAPESIEGFMAFKERRSPDWVHPELRIDGRL

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=249, Percent_Identity=28.1124497991968, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI194097323, Length=248, Percent_Identity=25.8064516129032, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI70995211, Length=182, Percent_Identity=30.2197802197802, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI68989263, Length=186, Percent_Identity=30.6451612903226, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI45643119, Length=218, Percent_Identity=25.2293577981651, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI260275230, Length=218, Percent_Identity=25.2293577981651, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI260274832, Length=218, Percent_Identity=25.2293577981651, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI20127408, Length=188, Percent_Identity=26.5957446808511, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI221142681, Length=269, Percent_Identity=33.457249070632, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1787659, Length=253, Percent_Identity=28.8537549407115, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1788597, Length=249, Percent_Identity=30.1204819277108, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1788682, Length=194, Percent_Identity=28.3505154639175, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082183, Length=186, Percent_Identity=29.0322580645161, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787660, Length=258, Percent_Identity=29.0697674418605, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1790281, Length=206, Percent_Identity=26.2135922330097, Blast_Score=61, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI25145438, Length=255, Percent_Identity=28.2352941176471, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17554946, Length=242, Percent_Identity=26.0330578512397, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=215, Percent_Identity=24.6511627906977, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17536985, Length=216, Percent_Identity=27.7777777777778, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI25144276, Length=181, Percent_Identity=27.6243093922652, Blast_Score=64, Evalue=1e-10,
Organism=Drosophila melanogaster, GI20129971, Length=248, Percent_Identity=26.2096774193548, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24653477, Length=248, Percent_Identity=26.2096774193548, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19920382, Length=211, Percent_Identity=28.9099526066351, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21357171, Length=256, Percent_Identity=28.515625, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24653139, Length=285, Percent_Identity=25.6140350877193, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 29547; Mature: 29416

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEHDAPRPPDGDWLGTPYLRFTREGAFGVCTLDRPEARNAMTPAMYFGIKYAVNHVGTD
CCCCCCCCCCCCCCCCCCHHEEECCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCC
PDLAGLLITGTGDVFAPGGDMGGGGADNWLTFGSALGMDALPFDTLRQSAKPVVAAVNGL
CCCCEEEEECCCCEECCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHH
CQGGGMQIALCSDMAVVSDRATFRVPELYRGIADTYYSHMLARLIGPVRTRDLMFTGRTL
CCCCCEEEEEECCCHHHCCCCCEECHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEECCCH
SAAEALDWGLVARVVPHDDLHDAAREVLAQCCRTAPHARSVIKSSLDNYIGLYDRIGMQS
HHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHH
SLGAPESIEGFMAFKERRSPDWVHPELRIDGRL
CCCCCHHHHHHHHHHHCCCCCCCCCCEEECCCC
>Mature Secondary Structure 
SEHDAPRPPDGDWLGTPYLRFTREGAFGVCTLDRPEARNAMTPAMYFGIKYAVNHVGTD
CCCCCCCCCCCCCCCCCHHEEECCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCC
PDLAGLLITGTGDVFAPGGDMGGGGADNWLTFGSALGMDALPFDTLRQSAKPVVAAVNGL
CCCCEEEEECCCCEECCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHH
CQGGGMQIALCSDMAVVSDRATFRVPELYRGIADTYYSHMLARLIGPVRTRDLMFTGRTL
CCCCCEEEEEECCCHHHCCCCCEECHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEECCCH
SAAEALDWGLVARVVPHDDLHDAAREVLAQCCRTAPHARSVIKSSLDNYIGLYDRIGMQS
HHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHH
SLGAPESIEGFMAFKERRSPDWVHPELRIDGRL
CCCCCHHHHHHHHHHHCCCCCCCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]