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The map label for this gene is tgs1 [H]

Identifier: 119869646

GI number: 119869646

Start: 3801912

End: 3803300

Strand: Direct

Name: tgs1 [H]

Synonym: Mkms_3614

Alternate gene names: 119869646

Gene position: 3801912-3803300 (Clockwise)

Preceding gene: 119869645

Following gene: 119869647

Centisome position: 66.27

GC content: 70.7

Gene sequence:

>1389_bases
GTGGAGCACCTGAAGACCCTGGACGCGGGCTTCCTCGAGGCCGAGGACGCGGACCCGCACGTGAGCCTCGCAATCGGCGG
CGTCGCCGTCCTCGACGGCCCGATGCCCGACTTCGCCGCACTGACGGCCACCCTCACCGAACGGCTGACGGCCGTGCCGC
GGCTCACGCAGCTCGTCCACACCCGTCCGCTGGACATTGGTGCGCCGGAGTGGGTGCCCGACGAACACTTCGACATCTCC
CATCACATCCGGCGTGCGGCCCTGCCGCGACCGGGTGGCGATGCGGATCTCGACCGCCTGGTCGCCGACATCATGGAGCG
CCGGCTCGACCGGGCCCGGCCGCTGTGGGAGTGCTGGGTGATCGAGGGGTTGTCGCGCCACCGGTGGGCGGTGCTGATGA
AGGTGCACCACTGCCTCGCCGACGGTATCGCCGCGACGCAGATACTGACCGCACTGTGTGACGACGGGCCCACCGCGGCG
CTGCGCCCGTATCCGCGGGCGGTGCCGTCGCCGGGCTGGTCGCTGCGCATCGATCCCGTCGGCTGGATGTCGAGCGCGGT
GCGCACATCGTTGGGACTGACCACCGCGGCGGTCCGCGCGGTCCGTGGCGCGATCGACCTCGCGGGCGGCGTGCTGCGGC
CGGCGCCCTCGTCGGTCCTGAACGGCCCGGTGGGCAGCATGCGCCGATACGCCGCCGTCGACGTGTCGCTGCCCGATGTG
CGCCGCGTCTGCGACGAGTTCGGGGTCACGCTCAACGACGTGGCACTGGCCGCCATCACCCACAGCTACCGCGAGCTGCT
GATCCGACGCGGCGAGAACCCCGGTCGTGACTCGCTGCGGACGCTGGTGCCCGTCTCGGTTCGTCCGGCAGACGCCATGG
ACGAACCGGACAACCGGGTGTCGTTGTTGCTGCCCCGCCTGCCGGTCGACAAGGCCGATCCGCTCCACCAGCTGCAGGCG
GTGCACCGCAGGCTCACGTACGTGAAGGGCAGCGGACAGCGGCAGGCCGGAAGTGCCGTCATGTCGGCGGCGAATGCGAT
CCCCTTCGCGGTGACGGCATGGGCGGTCCGGAACCTGACGCGGTTGCCGCAGCGCGGTGTCGTCGCGCTGGCGACCAATG
TTCCCGGCCCGCAGGAGGAACTGACGATCATGGGCCGCAAAGTCGGGCGGCTGTTGCCGATCCCGCCGATCGCCCTCCAA
TTACGCACGGGCATAGCGATTCTCAGCTATGCCGACAAACTGTTCTTCGGGATCATCAGCGACTACGACGCGGTGCCCGA
CGTCGACGAGCTGGCCCGCGGGATCGCGCAGGGAGTCGGGCGCCTCGCCGACCTCGCCTCCGTCCCGCATCGGTCGACAC
CGCTTGGCACGCTGGCGCTGGTGACCTGA

Upstream 100 bases:

>100_bases
GCGGATCGACGGGCGGTTGTGACCGCGGCATAGCTCAACTGGTTGAACTCGGGACCAATGGCCCTGGTGTGCCGGCCGAA
CGGAATGCGACGTTCGGTGC

Downstream 100 bases:

>100_bases
CACCTCCTTGTTGGCGCGAGGGTGTGCGCACGCGCGCTGACTCTCGAAACCTCTTTCGTCACAGTGGTTTCCACAGTCAC
ATCGCCGGCGGTTTTGTCAG

Product: diacylglycerol O-acyltransferase

Products: NA

Alternate protein names: TGS1; Probable triacylglycerol synthase tgs1 [H]

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MEHLKTLDAGFLEAEDADPHVSLAIGGVAVLDGPMPDFAALTATLTERLTAVPRLTQLVHTRPLDIGAPEWVPDEHFDIS
HHIRRAALPRPGGDADLDRLVADIMERRLDRARPLWECWVIEGLSRHRWAVLMKVHHCLADGIAATQILTALCDDGPTAA
LRPYPRAVPSPGWSLRIDPVGWMSSAVRTSLGLTTAAVRAVRGAIDLAGGVLRPAPSSVLNGPVGSMRRYAAVDVSLPDV
RRVCDEFGVTLNDVALAAITHSYRELLIRRGENPGRDSLRTLVPVSVRPADAMDEPDNRVSLLLPRLPVDKADPLHQLQA
VHRRLTYVKGSGQRQAGSAVMSAANAIPFAVTAWAVRNLTRLPQRGVVALATNVPGPQEELTIMGRKVGRLLPIPPIALQ
LRTGIAILSYADKLFFGIISDYDAVPDVDELARGIAQGVGRLADLASVPHRSTPLGTLALVT

Sequences:

>Translated_462_residues
MEHLKTLDAGFLEAEDADPHVSLAIGGVAVLDGPMPDFAALTATLTERLTAVPRLTQLVHTRPLDIGAPEWVPDEHFDIS
HHIRRAALPRPGGDADLDRLVADIMERRLDRARPLWECWVIEGLSRHRWAVLMKVHHCLADGIAATQILTALCDDGPTAA
LRPYPRAVPSPGWSLRIDPVGWMSSAVRTSLGLTTAAVRAVRGAIDLAGGVLRPAPSSVLNGPVGSMRRYAAVDVSLPDV
RRVCDEFGVTLNDVALAAITHSYRELLIRRGENPGRDSLRTLVPVSVRPADAMDEPDNRVSLLLPRLPVDKADPLHQLQA
VHRRLTYVKGSGQRQAGSAVMSAANAIPFAVTAWAVRNLTRLPQRGVVALATNVPGPQEELTIMGRKVGRLLPIPPIALQ
LRTGIAILSYADKLFFGIISDYDAVPDVDELARGIAQGVGRLADLASVPHRSTPLGTLALVT
>Mature_462_residues
MEHLKTLDAGFLEAEDADPHVSLAIGGVAVLDGPMPDFAALTATLTERLTAVPRLTQLVHTRPLDIGAPEWVPDEHFDIS
HHIRRAALPRPGGDADLDRLVADIMERRLDRARPLWECWVIEGLSRHRWAVLMKVHHCLADGIAATQILTALCDDGPTAA
LRPYPRAVPSPGWSLRIDPVGWMSSAVRTSLGLTTAAVRAVRGAIDLAGGVLRPAPSSVLNGPVGSMRRYAAVDVSLPDV
RRVCDEFGVTLNDVALAAITHSYRELLIRRGENPGRDSLRTLVPVSVRPADAMDEPDNRVSLLLPRLPVDKADPLHQLQA
VHRRLTYVKGSGQRQAGSAVMSAANAIPFAVTAWAVRNLTRLPQRGVVALATNVPGPQEELTIMGRKVGRLLPIPPIALQ
LRTGIAILSYADKLFFGIISDYDAVPDVDELARGIAQGVGRLADLASVPHRSTPLGTLALVT

Specific function: Upon expression in E.coli functions as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Prefers C(26:0)-CoA over C(18:1)-CoA. TG synthesis activity increases in M.tuberculosis upon oxygen depletion and NO

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014292
- InterPro:   IPR004255 [H]

Pfam domain/function: PF03007 UPF0089 [H]

EC number: =2.3.1.20 [H]

Molecular weight: Translated: 49796; Mature: 49796

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS00041 HTH_ARAC_FAMILY_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHLKTLDAGFLEAEDADPHVSLAIGGVAVLDGPMPDFAALTATLTERLTAVPRLTQLVH
CCCHHHHCCCCCCCCCCCCCEEEEECCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TRPLDIGAPEWVPDEHFDISHHIRRAALPRPGGDADLDRLVADIMERRLDRARPLWECWV
CCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHH
IEGLSRHRWAVLMKVHHCLADGIAATQILTALCDDGPTAALRPYPRAVPSPGWSLRIDPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCH
GWMSSAVRTSLGLTTAAVRAVRGAIDLAGGVLRPAPSSVLNGPVGSMRRYAAVDVSLPDV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHEECCCCHHH
RRVCDEFGVTLNDVALAAITHSYRELLIRRGENPGRDSLRTLVPVSVRPADAMDEPDNRV
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCE
SLLLPRLPVDKADPLHQLQAVHRRLTYVKGSGQRQAGSAVMSAANAIPFAVTAWAVRNLT
EEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHH
RLPQRGVVALATNVPGPQEELTIMGRKVGRLLPIPPIALQLRTGIAILSYADKLFFGIIS
HCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DYDAVPDVDELARGIAQGVGRLADLASVPHRSTPLGTLALVT
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEC
>Mature Secondary Structure
MEHLKTLDAGFLEAEDADPHVSLAIGGVAVLDGPMPDFAALTATLTERLTAVPRLTQLVH
CCCHHHHCCCCCCCCCCCCCEEEEECCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TRPLDIGAPEWVPDEHFDISHHIRRAALPRPGGDADLDRLVADIMERRLDRARPLWECWV
CCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHH
IEGLSRHRWAVLMKVHHCLADGIAATQILTALCDDGPTAALRPYPRAVPSPGWSLRIDPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCH
GWMSSAVRTSLGLTTAAVRAVRGAIDLAGGVLRPAPSSVLNGPVGSMRRYAAVDVSLPDV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHEECCCCHHH
RRVCDEFGVTLNDVALAAITHSYRELLIRRGENPGRDSLRTLVPVSVRPADAMDEPDNRV
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCE
SLLLPRLPVDKADPLHQLQAVHRRLTYVKGSGQRQAGSAVMSAANAIPFAVTAWAVRNLT
EEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHH
RLPQRGVVALATNVPGPQEELTIMGRKVGRLLPIPPIALQLRTGIAILSYADKLFFGIIS
HCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DYDAVPDVDELARGIAQGVGRLADLASVPHRSTPLGTLALVT
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]