Click here to switch to the map view.

The map label for this gene is dagK [H]

Identifier: 119869469

GI number: 119869469

Start: 3605930

End: 3606841

Strand: Direct

Name: dagK [H]

Synonym: Mkms_3437

Alternate gene names: 119869469

Gene position: 3605930-3606841 (Clockwise)

Preceding gene: 119869468

Following gene: 119869474

Centisome position: 62.85

GC content: 71.71

Gene sequence:

>912_bases
ATGAGCCCGATGACCATCGGCCACGTCACGGTGCTGACGAACCCGCTGTCGGGCCACGGCAACGCCCCGCACGCCACCGA
ACGCGCCGTCGCCCGGTTCCAGCGGCGTGGTGTCGACGTGACGGCGATCGTCGGGACCGACGCCGCTCACGCGCGCCGTC
TGGTAGACGAGGCGCTGGCCGGCGGGACCGACGCGCTCGTCGTGGTCGGCGGGGACGGCGTCATCTCACTGGCCCTGCAG
GCGCTGGCACACGGCGACGTCCCGCTGGGCATCGTGCCGGCGGGCACCGGCAACGACCATGCCCGCGAATACCGTCTGCC
GACCGGAGACCCGGAGGCGGCGGCCGACGTGATCGCCGACGGCCACACCGAGACCGTCGACCTCGGCCGGATCGACGACG
CGTCCGGCGGGCACAAGTGGTTCGGCACGGTGATGGCGGCCGGTTTCGACTCCCTGGTCAGCGACCGCACCAACCGGATG
CGCTGGCCGCACGGCCGGATGCGCTACAACGTGGCGATGGTCGCCGAACTGTCGAAGTTGCGACTGCTGCCGTTCCGGCT
GCGCTTCGACGGCGGCGAGGAGGTGCGTACCGATCTGACGCTGGCCGCGTTCGGCAACACCCGAAGCTACGGCGGCGGCA
TGCTGATCTGTCCCGACGCCGACCACACCGACGGGCTGCTCGACGTCACGATGGTGCATTCGGCCTCGCGGACCCGGCTG
ATCCGGTTGTTCCCGACGGTGTTCAAGGGGACCCACGTCAACCTCGACGACGTGACCACCGCCCGCGCGCGGGTCGTCGA
GGTGGACTGCCCGGGCATCAACGCCTACGCCGACGGCGACTACGCCTGTCCGCTCCCGGTCACGGTGTCCGCGGTGCCGG
GCGCGCTGCGCATCCTGGTGCCCGCCCCCTAG

Upstream 100 bases:

>100_bases
GACGAGGTCGGCGACCTGGGGGTGGAGATCCTGCGTGCGGTCAAGGCGACCCTCGACCCGGCCGGAATCCTCAACCCGGG
CAAGCTGATTCCATGACACG

Downstream 100 bases:

>100_bases
CGCGAGCAGACACAGAATCGCCCTTTCGCGACCGGGAAAGGGCGATTCTGTGTCTGCTCGCGGGGGAAGTCTGGCTACCC
GACTCCGCGCGACAGCCAGC

Product: diacylglycerol kinase

Products: NA

Alternate protein names: DAG kinase; DAGK [H]

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MSPMTIGHVTVLTNPLSGHGNAPHATERAVARFQRRGVDVTAIVGTDAAHARRLVDEALAGGTDALVVVGGDGVISLALQ
ALAHGDVPLGIVPAGTGNDHAREYRLPTGDPEAAADVIADGHTETVDLGRIDDASGGHKWFGTVMAAGFDSLVSDRTNRM
RWPHGRMRYNVAMVAELSKLRLLPFRLRFDGGEEVRTDLTLAAFGNTRSYGGGMLICPDADHTDGLLDVTMVHSASRTRL
IRLFPTVFKGTHVNLDDVTTARARVVEVDCPGINAYADGDYACPLPVTVSAVPGALRILVPAP

Sequences:

>Translated_303_residues
MSPMTIGHVTVLTNPLSGHGNAPHATERAVARFQRRGVDVTAIVGTDAAHARRLVDEALAGGTDALVVVGGDGVISLALQ
ALAHGDVPLGIVPAGTGNDHAREYRLPTGDPEAAADVIADGHTETVDLGRIDDASGGHKWFGTVMAAGFDSLVSDRTNRM
RWPHGRMRYNVAMVAELSKLRLLPFRLRFDGGEEVRTDLTLAAFGNTRSYGGGMLICPDADHTDGLLDVTMVHSASRTRL
IRLFPTVFKGTHVNLDDVTTARARVVEVDCPGINAYADGDYACPLPVTVSAVPGALRILVPAP
>Mature_302_residues
SPMTIGHVTVLTNPLSGHGNAPHATERAVARFQRRGVDVTAIVGTDAAHARRLVDEALAGGTDALVVVGGDGVISLALQA
LAHGDVPLGIVPAGTGNDHAREYRLPTGDPEAAADVIADGHTETVDLGRIDDASGGHKWFGTVMAAGFDSLVSDRTNRMR
WPHGRMRYNVAMVAELSKLRLLPFRLRFDGGEEVRTDLTLAAFGNTRSYGGGMLICPDADHTDGLLDVTMVHSASRTRLI
RLFPTVFKGTHVNLDDVTTARARVVEVDCPGINAYADGDYACPLPVTVSAVPGALRILVPAP

Specific function: Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is involved in the biosynthesis of phosphatidylinositol mannosides (PIMs), probably via a role in the biosynthesis of phosphatidylinositol (PI), a PIM precursor, which is derive

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Secreted, cell wall (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain [H]

Homologues:

Organism=Escherichia coli, GI1788402, Length=220, Percent_Identity=27.2727272727273, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005218
- InterPro:   IPR001206 [H]

Pfam domain/function: PF00781 DAGK_cat [H]

EC number: =2.7.1.107 [H]

Molecular weight: Translated: 32022; Mature: 31891

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPMTIGHVTVLTNPLSGHGNAPHATERAVARFQRRGVDVTAIVGTDAAHARRLVDEALA
CCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHC
GGTDALVVVGGDGVISLALQALAHGDVPLGIVPAGTGNDHAREYRLPTGDPEAAADVIAD
CCCCEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEECCCCCCHHHHHHHHCC
GHTETVDLGRIDDASGGHKWFGTVMAAGFDSLVSDRTNRMRWPHGRMRYNVAMVAELSKL
CCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHEEHHHHH
RLLPFRLRFDGGEEVRTDLTLAAFGNTRSYGGGMLICPDADHTDGLLDVTMVHSASRTRL
EEEEEEEEECCCCHHHHEEEEEEECCCCCCCCCEEEECCCCCCCCEEEEEEECCCCHHEE
IRLFPTVFKGTHVNLDDVTTARARVVEVDCPGINAYADGDYACPLPVTVSAVPGALRILV
EEEEHHHHCCCCCCHHCCCCCEEEEEEEECCCCCEECCCCEECCCCEEEECCCCEEEEEE
PAP
ECC
>Mature Secondary Structure 
SPMTIGHVTVLTNPLSGHGNAPHATERAVARFQRRGVDVTAIVGTDAAHARRLVDEALA
CCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHC
GGTDALVVVGGDGVISLALQALAHGDVPLGIVPAGTGNDHAREYRLPTGDPEAAADVIAD
CCCCEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEECCCCCCHHHHHHHHCC
GHTETVDLGRIDDASGGHKWFGTVMAAGFDSLVSDRTNRMRWPHGRMRYNVAMVAELSKL
CCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHEEHHHHH
RLLPFRLRFDGGEEVRTDLTLAAFGNTRSYGGGMLICPDADHTDGLLDVTMVHSASRTRL
EEEEEEEEECCCCHHHHEEEEEEECCCCCCCCCEEEECCCCCCCCEEEEEEECCCCHHEE
IRLFPTVFKGTHVNLDDVTTARARVVEVDCPGINAYADGDYACPLPVTVSAVPGALRILV
EEEEHHHHCCCCCCHHCCCCCEEEEEEEECCCCCEECCCCEECCCCEEEECCCCEEEEEE
PAP
ECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]