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The map label for this gene is paaF [C]

Identifier: 119867851

GI number: 119867851

Start: 1923322

End: 1924134

Strand: Reverse

Name: paaF [C]

Synonym: Mkms_1812

Alternate gene names: 119867851

Gene position: 1924134-1923322 (Counterclockwise)

Preceding gene: 119867852

Following gene: 119867850

Centisome position: 33.54

GC content: 66.91

Gene sequence:

>813_bases
ATGACCGACAGTTCGTACGACACGATCAAATACGAGGTCGACGGACACACCGCGACCATCACGCTGAACCGGCCCGATGC
GCTCAACGCGCTCAGCCCGCACATGATCACCGAACTGCGGGCGGCCTACGACGAAGCGGAGAACGACGACGACGTCTGGC
TGCTGATCGTGACGGCCACCGGGCGGGCCTTCTGCACCGGCGCCGACGTCAAGGAGATCCCCGAGGACGGCAAGGTGCTC
AACGAACGCGCCTTCCTGTCCACCTACGAACAGTGGGAGGCGCCGCAGGAGGGCACCCCGCCGTTCCGCCGGATGGCCAA
ACCGGTGCTCGCCGCGATCAACGGGATCTGCTGCGGCGCCGGGCTGGACTGGGTGACCACCGGTGACATCGTGATCGCCT
CCGAGCAGGCTACCTTTTTCGATCCACACGTCAGCATCGGGCTGGTCTCGGGGCGCGAGGTGGTGCGGCTGGCCCGTGTG
CTGCCCCGCTCGGTCGCGCTGCGCATGGCGATCGTGGGCAAACACGAGCGGATGAGCGCACAGCGGGCCTACGAACTCGG
CATGATCAGCGAGGTCGTCGAACACGACCGGCTCCTCGAACGCGCCCATGAGATCGCCGACCTGGTGACCCGCAATGCGC
CGTTGGCCGTCCGCGGCACCCGGTTGGCCATCCTCAAGGGTCTGGATCTGCCGTTGCACGAGGCCGAGATGCTCGGTGAG
GCGTTCCGTGAGCGGAACCTGCACACCGAGGACTCCCTGGAGGGGCCGAAGGCGTTCGTCGAGAAGCGCACCCCGGACTG
GAAGTGCCGATGA

Upstream 100 bases:

>100_bases
CGTACAGCGGATTTCAAAGAGGGTCTGGCCGCGTTCCGAGACAAGCGCGACCCGGACTTCCAAGGCCGATGACCCCCGAC
CACACCATCGAGGAGCGCGG

Downstream 100 bases:

>100_bases
CCTTCGAGACGATCACGCTCGACGTCGACGCCGCGGTCCACGTCGCGACCATCACGCTCAACCGCCCCGAGCGGCTCAAC
GCGTTCAACCGCACCATGTG

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MTDSSYDTIKYEVDGHTATITLNRPDALNALSPHMITELRAAYDEAENDDDVWLLIVTATGRAFCTGADVKEIPEDGKVL
NERAFLSTYEQWEAPQEGTPPFRRMAKPVLAAINGICCGAGLDWVTTGDIVIASEQATFFDPHVSIGLVSGREVVRLARV
LPRSVALRMAIVGKHERMSAQRAYELGMISEVVEHDRLLERAHEIADLVTRNAPLAVRGTRLAILKGLDLPLHEAEMLGE
AFRERNLHTEDSLEGPKAFVEKRTPDWKCR

Sequences:

>Translated_270_residues
MTDSSYDTIKYEVDGHTATITLNRPDALNALSPHMITELRAAYDEAENDDDVWLLIVTATGRAFCTGADVKEIPEDGKVL
NERAFLSTYEQWEAPQEGTPPFRRMAKPVLAAINGICCGAGLDWVTTGDIVIASEQATFFDPHVSIGLVSGREVVRLARV
LPRSVALRMAIVGKHERMSAQRAYELGMISEVVEHDRLLERAHEIADLVTRNAPLAVRGTRLAILKGLDLPLHEAEMLGE
AFRERNLHTEDSLEGPKAFVEKRTPDWKCR
>Mature_269_residues
TDSSYDTIKYEVDGHTATITLNRPDALNALSPHMITELRAAYDEAENDDDVWLLIVTATGRAFCTGADVKEIPEDGKVLN
ERAFLSTYEQWEAPQEGTPPFRRMAKPVLAAINGICCGAGLDWVTTGDIVIASEQATFFDPHVSIGLVSGREVVRLARVL
PRSVALRMAIVGKHERMSAQRAYELGMISEVVEHDRLLERAHEIADLVTRNAPLAVRGTRLAILKGLDLPLHEAEMLGEA
FRERNLHTEDSLEGPKAFVEKRTPDWKCR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=276, Percent_Identity=30.7971014492754, Blast_Score=127, Evalue=8e-30,
Organism=Homo sapiens, GI31542718, Length=261, Percent_Identity=31.4176245210728, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI70995211, Length=271, Percent_Identity=25.830258302583, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI37594469, Length=212, Percent_Identity=29.7169811320755, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI37594471, Length=201, Percent_Identity=29.8507462686567, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI116235487, Length=188, Percent_Identity=30.8510638297872, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI221307494, Length=195, Percent_Identity=28.2051282051282, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI4502327, Length=260, Percent_Identity=27.6923076923077, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI221136756, Length=195, Percent_Identity=28.2051282051282, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI221136753, Length=195, Percent_Identity=28.2051282051282, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI25453481, Length=235, Percent_Identity=24.6808510638298, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI51538573, Length=235, Percent_Identity=24.6808510638298, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI4757966, Length=235, Percent_Identity=24.6808510638298, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI51538581, Length=235, Percent_Identity=24.6808510638298, Blast_Score=68, Evalue=9e-12,
Organism=Escherichia coli, GI1787659, Length=259, Percent_Identity=33.2046332046332, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI221142681, Length=272, Percent_Identity=29.7794117647059, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI1788597, Length=272, Percent_Identity=27.2058823529412, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787660, Length=270, Percent_Identity=31.1111111111111, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87082183, Length=282, Percent_Identity=25.531914893617, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1788682, Length=211, Percent_Identity=29.8578199052133, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17554946, Length=273, Percent_Identity=33.6996336996337, Blast_Score=137, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI25145438, Length=254, Percent_Identity=32.2834645669291, Blast_Score=131, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17540306, Length=242, Percent_Identity=26.8595041322314, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17536985, Length=228, Percent_Identity=27.6315789473684, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17534483, Length=291, Percent_Identity=24.7422680412371, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17560910, Length=262, Percent_Identity=23.6641221374046, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17535521, Length=233, Percent_Identity=24.8927038626609, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17540714, Length=219, Percent_Identity=27.8538812785388, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI20129971, Length=270, Percent_Identity=31.8518518518519, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24653477, Length=270, Percent_Identity=31.8518518518519, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI19922422, Length=249, Percent_Identity=26.1044176706827, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19920382, Length=221, Percent_Identity=28.0542986425339, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24653139, Length=263, Percent_Identity=28.1368821292776, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI21357171, Length=263, Percent_Identity=25.4752851711027, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24650670, Length=283, Percent_Identity=24.3816254416961, Blast_Score=71, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 30002; Mature: 29871

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDSSYDTIKYEVDGHTATITLNRPDALNALSPHMITELRAAYDEAENDDDVWLLIVTAT
CCCCCCCEEEEEECCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEC
GRAFCTGADVKEIPEDGKVLNERAFLSTYEQWEAPQEGTPPFRRMAKPVLAAINGICCGA
CCEEECCCCHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCHHCCC
GLDWVTTGDIVIASEQATFFDPHVSIGLVSGREVVRLARVLPRSVALRMAIVGKHERMSA
CCCEEECCCEEEEECCCEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
QRAYELGMISEVVEHDRLLERAHEIADLVTRNAPLAVRGTRLAILKGLDLPLHEAEMLGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEEEEECCCCCHHHHHHHHH
AFRERNLHTEDSLEGPKAFVEKRTPDWKCR
HHHHCCCCCCCCCCCHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TDSSYDTIKYEVDGHTATITLNRPDALNALSPHMITELRAAYDEAENDDDVWLLIVTAT
CCCCCCEEEEEECCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEC
GRAFCTGADVKEIPEDGKVLNERAFLSTYEQWEAPQEGTPPFRRMAKPVLAAINGICCGA
CCEEECCCCHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCHHCCC
GLDWVTTGDIVIASEQATFFDPHVSIGLVSGREVVRLARVLPRSVALRMAIVGKHERMSA
CCCEEECCCEEEEECCCEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
QRAYELGMISEVVEHDRLLERAHEIADLVTRNAPLAVRGTRLAILKGLDLPLHEAEMLGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEEEEECCCCCHHHHHHHHH
AFRERNLHTEDSLEGPKAFVEKRTPDWKCR
HHHHCCCCCCCCCCCHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA