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The map label for this gene is menE [H]

Identifier: 119867849

GI number: 119867849

Start: 1921077

End: 1922543

Strand: Reverse

Name: menE [H]

Synonym: Mkms_1810

Alternate gene names: 119867849

Gene position: 1922543-1921077 (Counterclockwise)

Preceding gene: 119867850

Following gene: 119867848

Centisome position: 33.51

GC content: 73.14

Gene sequence:

>1467_bases
ATGGCCCCCCACCGCCTGAGCCGGCGCATCGCCCACGTCCTGGATCTGTGTCCCGAGGGCAACGCCGTGGAATACGACGG
GCAGTGGGTGACGTGGGCGCAGATCGGCGAGGCGGCCCGGCGGATCGCCGAACTCGTCACCCGGTACGGCGGTGACACCC
CGCAGGTGGGCATCCTGCTCCGCAACCGGCCGCCACAGGTCGCCGCGGTGCTGGGGGTGTTGCTCGCCGGCGGCACCGTG
GTGGTGGTCAACCCGTCCCGCGGGGACGACCGCACCCGCGCCGACATCACCGCCCTCGCGCTCCCGGTCCTCATCGGCGA
GCCGGACGATCTGGCCGCCCTGGTGACCCCGGCGCCCGCCACCACGGTGCTGTCGCTCACGGCGCTGTCGCAGGAACCCG
TCGTCGACGCCGCCGCACGGCGCAGGGCCGAGACGCGGCCCGGGGTGGCGGTGCGGATGTTGACCAGCGGCACCACTGGG
CCCCCCAAGCGGATCGACCTCACCTACGACATGCTCGCGCACAGCGTCCTGGGGCCCGAGCCGGACGGACCGCGTCGGGG
TATCGCGATCGTCAACGCCCCGCTCGTGCACATCGGCGGGGTGTTCCGGGTGCTGCAGTGCGTGGGCGAGGCGCGGTCGT
TCGCGCTGCTGGAGCGGTTCGAACTGCAGCGGTGGGCCGATGCGGTGCGCCGGCACCGGCCCGCTGCGGTGTCGCTGGTC
CCGGCCGCCCTGCGGATGGTGCTGCACTCCGACCTCACCCGCGACGACCTGGCCGGCATCCGCGCCGTCACCTCGGGCAC
CGCACCGCTGTCGGCCGACGACGCCGACGCGTTCACCGAGATGTTCGGCATCCCGGTGCTGACGTCCTACGCCGCAACGG
AATTCGGCGGCGGGGTGGCCGGCTGGACGCTGGCCGACCATCAGCGCCACTGGCACACCAAACGTGGCAGCGTCGGACGC
GCCAGCGGCGGGGCGCACCTGCGTGTCGTCGATGAGGACGGAACGCCGCTCGGCCCGGACCAACCCGGGCTGCTCGAGGT
CAAACCCGGCCAGCTGGGCCCCGGTGCGGACTGGATCCGCACCACCGACCTCGCCCGCATCGACGCCGACGGGTTCCTGT
GGATACTGGGCCGCGCGGATCAGGCCATCATCCGCGGTGGCTTCAAGGTGATGCCCGACGACGTGCGCACCGCGCTCGAA
GGGCATCCCGCGGTGCGGGGCGCCACCGTCGTCGGATGCCCGGACGACCGCCTCGGCGAAACACCCGTCGCAATGGTCGA
ATTGCGCGACGGGGCGACCACGAGCGTCGACGAGCTCACCGCATACCTGCGCGGCCGCCTCGCGCACTACGAGGTGCCGA
GCGAGATCGCGGTCGTCGACACGATCCCGCGGACCCCCTCGGGCAAGGCCGATCTGGGCGCGGTCCGGCGGTACTTCTCG
CCGGCGGTCACCGTCGACCATGGCTGA

Upstream 100 bases:

>100_bases
TGGAGCAGGGGTTGATCTACACCCGGTTGGGCAATCCCATCGGCCAGGCCGAGCTCGCCGCGCATCCGCCTGCGGCCCGC
CCGGACCCGAGGGTGCGGTG

Downstream 100 bases:

>100_bases
GCCGCACACCGTCGCCGCCGTGCTGGGGCGCTGGGCGGTCGACCGCCCAGCGCACCCGCTGCTGATCTGCGACGCCGACC
GCCTCGACTACGGGCAGGCC

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MAPHRLSRRIAHVLDLCPEGNAVEYDGQWVTWAQIGEAARRIAELVTRYGGDTPQVGILLRNRPPQVAAVLGVLLAGGTV
VVVNPSRGDDRTRADITALALPVLIGEPDDLAALVTPAPATTVLSLTALSQEPVVDAAARRRAETRPGVAVRMLTSGTTG
PPKRIDLTYDMLAHSVLGPEPDGPRRGIAIVNAPLVHIGGVFRVLQCVGEARSFALLERFELQRWADAVRRHRPAAVSLV
PAALRMVLHSDLTRDDLAGIRAVTSGTAPLSADDADAFTEMFGIPVLTSYAATEFGGGVAGWTLADHQRHWHTKRGSVGR
ASGGAHLRVVDEDGTPLGPDQPGLLEVKPGQLGPGADWIRTTDLARIDADGFLWILGRADQAIIRGGFKVMPDDVRTALE
GHPAVRGATVVGCPDDRLGETPVAMVELRDGATTSVDELTAYLRGRLAHYEVPSEIAVVDTIPRTPSGKADLGAVRRYFS
PAVTVDHG

Sequences:

>Translated_488_residues
MAPHRLSRRIAHVLDLCPEGNAVEYDGQWVTWAQIGEAARRIAELVTRYGGDTPQVGILLRNRPPQVAAVLGVLLAGGTV
VVVNPSRGDDRTRADITALALPVLIGEPDDLAALVTPAPATTVLSLTALSQEPVVDAAARRRAETRPGVAVRMLTSGTTG
PPKRIDLTYDMLAHSVLGPEPDGPRRGIAIVNAPLVHIGGVFRVLQCVGEARSFALLERFELQRWADAVRRHRPAAVSLV
PAALRMVLHSDLTRDDLAGIRAVTSGTAPLSADDADAFTEMFGIPVLTSYAATEFGGGVAGWTLADHQRHWHTKRGSVGR
ASGGAHLRVVDEDGTPLGPDQPGLLEVKPGQLGPGADWIRTTDLARIDADGFLWILGRADQAIIRGGFKVMPDDVRTALE
GHPAVRGATVVGCPDDRLGETPVAMVELRDGATTSVDELTAYLRGRLAHYEVPSEIAVVDTIPRTPSGKADLGAVRRYFS
PAVTVDHG
>Mature_487_residues
APHRLSRRIAHVLDLCPEGNAVEYDGQWVTWAQIGEAARRIAELVTRYGGDTPQVGILLRNRPPQVAAVLGVLLAGGTVV
VVNPSRGDDRTRADITALALPVLIGEPDDLAALVTPAPATTVLSLTALSQEPVVDAAARRRAETRPGVAVRMLTSGTTGP
PKRIDLTYDMLAHSVLGPEPDGPRRGIAIVNAPLVHIGGVFRVLQCVGEARSFALLERFELQRWADAVRRHRPAAVSLVP
AALRMVLHSDLTRDDLAGIRAVTSGTAPLSADDADAFTEMFGIPVLTSYAATEFGGGVAGWTLADHQRHWHTKRGSVGRA
SGGAHLRVVDEDGTPLGPDQPGLLEVKPGQLGPGADWIRTTDLARIDADGFLWILGRADQAIIRGGFKVMPDDVRTALEG
HPAVRGATVVGCPDDRLGETPVAMVELRDGATTSVDELTAYLRGRLAHYEVPSEIAVVDTIPRTPSGKADLGAVRRYFSP
AVTVDHG

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=469, Percent_Identity=26.4392324093817, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI187761345, Length=511, Percent_Identity=27.0058708414873, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI187761343, Length=511, Percent_Identity=27.0058708414873, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI38505220, Length=367, Percent_Identity=24.2506811989101, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI122937307, Length=259, Percent_Identity=26.6409266409266, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI42544132, Length=287, Percent_Identity=24.7386759581882, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI115511026, Length=508, Percent_Identity=22.0472440944882, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI157311624, Length=354, Percent_Identity=24.0112994350282, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI157311622, Length=354, Percent_Identity=24.0112994350282, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI58082049, Length=355, Percent_Identity=23.3802816901408, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI221142682, Length=342, Percent_Identity=26.0233918128655, Blast_Score=105, Evalue=9e-24,
Organism=Escherichia coli, GI145693145, Length=399, Percent_Identity=28.3208020050125, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788107, Length=345, Percent_Identity=24.6376811594203, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1786810, Length=421, Percent_Identity=23.9904988123515, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790505, Length=356, Percent_Identity=25, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1786529, Length=281, Percent_Identity=25.2669039145907, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17558820, Length=359, Percent_Identity=25.6267409470752, Blast_Score=111, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI32563687, Length=344, Percent_Identity=26.1627906976744, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17531443, Length=355, Percent_Identity=26.4788732394366, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17559526, Length=502, Percent_Identity=22.5099601593625, Blast_Score=102, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17560140, Length=266, Percent_Identity=24.0601503759398, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17560308, Length=381, Percent_Identity=28.8713910761155, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI71994694, Length=526, Percent_Identity=23.384030418251, Blast_Score=78, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI71994703, Length=526, Percent_Identity=23.384030418251, Blast_Score=78, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI71994690, Length=526, Percent_Identity=23.384030418251, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17538037, Length=366, Percent_Identity=21.5846994535519, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17557194, Length=463, Percent_Identity=21.3822894168467, Blast_Score=76, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI71985884, Length=410, Percent_Identity=22.9268292682927, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=339, Percent_Identity=25.9587020648968, Blast_Score=113, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6323182, Length=287, Percent_Identity=27.1777003484321, Blast_Score=73, Evalue=8e-14,
Organism=Drosophila melanogaster, GI18859661, Length=511, Percent_Identity=27.3972602739726, Blast_Score=138, Evalue=7e-33,
Organism=Drosophila melanogaster, GI24581924, Length=373, Percent_Identity=26.2734584450402, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI21355181, Length=347, Percent_Identity=27.0893371757925, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21356441, Length=349, Percent_Identity=23.2091690544413, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24653035, Length=489, Percent_Identity=23.9263803680982, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648253, Length=466, Percent_Identity=21.244635193133, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI161076582, Length=486, Percent_Identity=23.0452674897119, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24648255, Length=357, Percent_Identity=22.6890756302521, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21356947, Length=506, Percent_Identity=22.9249011857708, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI19922652, Length=491, Percent_Identity=21.5885947046843, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24656500, Length=513, Percent_Identity=23.1968810916179, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648260, Length=487, Percent_Identity=22.9979466119097, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 51877; Mature: 51746

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPHRLSRRIAHVLDLCPEGNAVEYDGQWVTWAQIGEAARRIAELVTRYGGDTPQVGILL
CCCHHHHHHHHHHHHHCCCCCEEEECCCEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
RNRPPQVAAVLGVLLAGGTVVVVNPSRGDDRTRADITALALPVLIGEPDDLAALVTPAPA
CCCCCHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHEECCCCCCEEEECCCCH
TTVLSLTALSQEPVVDAAARRRAETRPGVAVRMLTSGTTGPPKRIDLTYDMLAHSVLGPE
HHHEEEECCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEEHHHHHHHHHCCCC
PDGPRRGIAIVNAPLVHIGGVFRVLQCVGEARSFALLERFELQRWADAVRRHRPAAVSLV
CCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
PAALRMVLHSDLTRDDLAGIRAVTSGTAPLSADDADAFTEMFGIPVLTSYAATEFGGGVA
HHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCEE
GWTLADHQRHWHTKRGSVGRASGGAHLRVVDEDGTPLGPDQPGLLEVKPGQLGPGADWIR
CCEECCCHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEECCCCCCCCCCHHC
TTDLARIDADGFLWILGRADQAIIRGGFKVMPDDVRTALEGHPAVRGATVVGCPDDRLGE
CCCCEEECCCCEEEEEECCCHHHHHCCCEECCHHHHHHHCCCCCCCCCEEEECCCCCCCC
TPVAMVELRDGATTSVDELTAYLRGRLAHYEVPSEIAVVDTIPRTPSGKADLGAVRRYFS
CCEEEEEECCCCCCCHHHHHHHHHHHHHEECCCCCEEEEECCCCCCCCCCHHHHHHHHCC
PAVTVDHG
CCEEECCC
>Mature Secondary Structure 
APHRLSRRIAHVLDLCPEGNAVEYDGQWVTWAQIGEAARRIAELVTRYGGDTPQVGILL
CCHHHHHHHHHHHHHCCCCCEEEECCCEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
RNRPPQVAAVLGVLLAGGTVVVVNPSRGDDRTRADITALALPVLIGEPDDLAALVTPAPA
CCCCCHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHEECCCCCCEEEECCCCH
TTVLSLTALSQEPVVDAAARRRAETRPGVAVRMLTSGTTGPPKRIDLTYDMLAHSVLGPE
HHHEEEECCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEEHHHHHHHHHCCCC
PDGPRRGIAIVNAPLVHIGGVFRVLQCVGEARSFALLERFELQRWADAVRRHRPAAVSLV
CCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
PAALRMVLHSDLTRDDLAGIRAVTSGTAPLSADDADAFTEMFGIPVLTSYAATEFGGGVA
HHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCEE
GWTLADHQRHWHTKRGSVGRASGGAHLRVVDEDGTPLGPDQPGLLEVKPGQLGPGADWIR
CCEECCCHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEECCCCCCCCCCHHC
TTDLARIDADGFLWILGRADQAIIRGGFKVMPDDVRTALEGHPAVRGATVVGCPDDRLGE
CCCCEEECCCCEEEEEECCCHHHHHCCCEECCHHHHHHHCCCCCCCCCEEEECCCCCCCC
TPVAMVELRDGATTSVDELTAYLRGRLAHYEVPSEIAVVDTIPRTPSGKADLGAVRRYFS
CCEEEEEECCCCCCCHHHHHHHHHHHHHEECCCCCEEEEECCCCCCCCCCHHHHHHHHCC
PAVTVDHG
CCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA